TY  - JOUR
AU  - Vitorović, Duško
AU  - Božičković, Ivana
AU  - Lukić, Miloš
AU  - Relić, Renata
AU  - Škrbić, Zdenka
AU  - Petričević, Veselin
AU  - Lazarević Macanović, Mirjana
AU  - Krstić, Nikola
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3001
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6399
AB  - The first few weeks after broilers hatch are the period of most intense bone growthand development, and the time when they are most susceptible to the in fluence ofvarious external and internal factors. Research in the recent decades has focused on theinvolvement of melatonin in bone development during early life in chickens. Melatoninrelease from the pineal gland has a circadian rhythm, with the highest levels circulatingduring the night and decreasing during the light phase of the day. Various types oflighting are used in intensive broiler production. In this study, the effects of melatoninon the tibial structure and growth of broilers were investigated. During the first twoweeks of life, two groups of chickens were kept under continuous light and fed thesame diet, with the experimental group receiving melatonin in the amount of 0.03 g/kg of feed. The results obtained showed that the addition of melatonin in the diet hadpositive effects on the development and growth of the tibia, which was expressed ina signi ficantly greater thickness of the diaphysis and cortical bone of the diaphysis,higher breaking force and higher values of alkaline phosphatase activity. The corticalbone mineral density of the tibia did not differ signi ficantly between the groups ofchicken.
T2  - Acta Veterinaria
T2  - Acta Veterinaria
T1  - Tibia growth and development in broiler chicks reared under continuous light and melatonin dietary supplementation during the first two weeks of life
EP  - 270
IS  - 2
SP  - 262
VL  - 73
DO  - 10.2478/acve-2023-0020
ER  - 

@article{
author = "Vitorović, Duško and Božičković, Ivana and Lukić, Miloš and Relić, Renata and Škrbić, Zdenka and Petričević, Veselin and Lazarević Macanović, Mirjana and Krstić, Nikola",
year = "2023",
abstract = "The first few weeks after broilers hatch are the period of most intense bone growthand development, and the time when they are most susceptible to the in fluence ofvarious external and internal factors. Research in the recent decades has focused on theinvolvement of melatonin in bone development during early life in chickens. Melatoninrelease from the pineal gland has a circadian rhythm, with the highest levels circulatingduring the night and decreasing during the light phase of the day. Various types oflighting are used in intensive broiler production. In this study, the effects of melatoninon the tibial structure and growth of broilers were investigated. During the first twoweeks of life, two groups of chickens were kept under continuous light and fed thesame diet, with the experimental group receiving melatonin in the amount of 0.03 g/kg of feed. The results obtained showed that the addition of melatonin in the diet hadpositive effects on the development and growth of the tibia, which was expressed ina signi ficantly greater thickness of the diaphysis and cortical bone of the diaphysis,higher breaking force and higher values of alkaline phosphatase activity. The corticalbone mineral density of the tibia did not differ signi ficantly between the groups ofchicken.",
journal = "Acta Veterinaria, Acta Veterinaria",
title = "Tibia growth and development in broiler chicks reared under continuous light and melatonin dietary supplementation during the first two weeks of life",
pages = "270-262",
number = "2",
volume = "73",
doi = "10.2478/acve-2023-0020"
}

Vitorović, D., Božičković, I., Lukić, M., Relić, R., Škrbić, Z., Petričević, V., Lazarević Macanović, M.,& Krstić, N.. (2023). Tibia growth and development in broiler chicks reared under continuous light and melatonin dietary supplementation during the first two weeks of life. in Acta Veterinaria, 73(2), 262-270.
https://doi.org/10.2478/acve-2023-0020

Vitorović D, Božičković I, Lukić M, Relić R, Škrbić Z, Petričević V, Lazarević Macanović M, Krstić N. Tibia growth and development in broiler chicks reared under continuous light and melatonin dietary supplementation during the first two weeks of life. in Acta Veterinaria. 2023;73(2):262-270.
doi:10.2478/acve-2023-0020 .

Vitorović, Duško, Božičković, Ivana, Lukić, Miloš, Relić, Renata, Škrbić, Zdenka, Petričević, Veselin, Lazarević Macanović, Mirjana, Krstić, Nikola, "Tibia growth and development in broiler chicks reared under continuous light and melatonin dietary supplementation during the first two weeks of life" in Acta Veterinaria, 73, no. 2 (2023):262-270,
https://doi.org/10.2478/acve-2023-0020 . .

TY  - JOUR
AU  - Relić, Renata
AU  - Škrbić, Zdenka
AU  - Božičković, Ivana
AU  - Lukić, Miloš
AU  - Petričević, Veselin
AU  - Delić, Nikola
AU  - Bondžić, Aleksandra
AU  - Vitorović, Duško
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6236
AB  - Intensive broiler chicken production involves different lighting regimes, which affects a natural cycle of secretion of melatonin, a hormone included in multiple physiological processes in a bird's body. This research aimed to determine the effects of dietary melatonin supplementation during the first two weeks of broiler chickens' life, bred under constant 24 h lighting, on their health and some hematological, biochemical, and production parameters. The study lasted 6 weeks. Three hundred and twenty 1-day chickens were divided into two groups (control-C and experimental-M), with four replications and 40 chickens in each replica. In the first two weeks (the first phase of the study), a continuous lighting mode 24L: 0D was applied. Group M was receiving a diet supplemented with 30 mg/kg synthetic melatonin only during the first phase. Group C had a diet without melatonin supplementation throughout the whole study. Melatonin addition had a statistically significant effect (P<0.01) on the mean values of body weight and daily weight gain at the end of the 1st, 2nd, 3rd, 4th, 5th, and 6th week. The chickens of the experimental group have had a significantly higher value of the European Production Efficiency Factor (EPEF) (P<0.05). The results presented in this paper indicate a direct benefit in stress relief in broilers and a value of the organism's antioxidant system promotion, manifested by improved production performance and fattening efficiency. © 2022, Ankara University. All rights reserved.
T2  - Ankara Universitesi Veteriner Fakultesi Dergisi
T2  - Ankara Universitesi Veteriner Fakultesi Dergisi
T1  - Effects of dietary melatonin on broiler chicken exposed to continuous lighting during the first two weeks of life
EP  - 366
IS  - 4
SP  - 361
VL  - 69
DO  - 10.33988/auvfd.866702
ER  - 

@article{
author = "Relić, Renata and Škrbić, Zdenka and Božičković, Ivana and Lukić, Miloš and Petričević, Veselin and Delić, Nikola and Bondžić, Aleksandra and Vitorović, Duško",
year = "2022",
abstract = "Intensive broiler chicken production involves different lighting regimes, which affects a natural cycle of secretion of melatonin, a hormone included in multiple physiological processes in a bird's body. This research aimed to determine the effects of dietary melatonin supplementation during the first two weeks of broiler chickens' life, bred under constant 24 h lighting, on their health and some hematological, biochemical, and production parameters. The study lasted 6 weeks. Three hundred and twenty 1-day chickens were divided into two groups (control-C and experimental-M), with four replications and 40 chickens in each replica. In the first two weeks (the first phase of the study), a continuous lighting mode 24L: 0D was applied. Group M was receiving a diet supplemented with 30 mg/kg synthetic melatonin only during the first phase. Group C had a diet without melatonin supplementation throughout the whole study. Melatonin addition had a statistically significant effect (P<0.01) on the mean values of body weight and daily weight gain at the end of the 1st, 2nd, 3rd, 4th, 5th, and 6th week. The chickens of the experimental group have had a significantly higher value of the European Production Efficiency Factor (EPEF) (P<0.05). The results presented in this paper indicate a direct benefit in stress relief in broilers and a value of the organism's antioxidant system promotion, manifested by improved production performance and fattening efficiency. © 2022, Ankara University. All rights reserved.",
journal = "Ankara Universitesi Veteriner Fakultesi Dergisi, Ankara Universitesi Veteriner Fakultesi Dergisi",
title = "Effects of dietary melatonin on broiler chicken exposed to continuous lighting during the first two weeks of life",
pages = "366-361",
number = "4",
volume = "69",
doi = "10.33988/auvfd.866702"
}

Relić, R., Škrbić, Z., Božičković, I., Lukić, M., Petričević, V., Delić, N., Bondžić, A.,& Vitorović, D.. (2022). Effects of dietary melatonin on broiler chicken exposed to continuous lighting during the first two weeks of life. in Ankara Universitesi Veteriner Fakultesi Dergisi, 69(4), 361-366.
https://doi.org/10.33988/auvfd.866702

Relić R, Škrbić Z, Božičković I, Lukić M, Petričević V, Delić N, Bondžić A, Vitorović D. Effects of dietary melatonin on broiler chicken exposed to continuous lighting during the first two weeks of life. in Ankara Universitesi Veteriner Fakultesi Dergisi. 2022;69(4):361-366.
doi:10.33988/auvfd.866702 .

Relić, Renata, Škrbić, Zdenka, Božičković, Ivana, Lukić, Miloš, Petričević, Veselin, Delić, Nikola, Bondžić, Aleksandra, Vitorović, Duško, "Effects of dietary melatonin on broiler chicken exposed to continuous lighting during the first two weeks of life" in Ankara Universitesi Veteriner Fakultesi Dergisi, 69, no. 4 (2022):361-366,
https://doi.org/10.33988/auvfd.866702 . .

TY  - JOUR
AU  - Núñez, Yolanda
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - García-Casco, Juan M.
AU  - Čandek-Potokar, Marjeta
AU  - Benítez, Rita
AU  - Radojković, Dragan
AU  - Lukić, Miloš
AU  - Gogić, Marija
AU  - Muñoz, María
AU  - Fontanesi, Luca
AU  - Óvilo, Cristina
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5827
AB  - This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.
PB  - MDPI AG
T2  - Animals
T1  - Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
EP  - 24
IS  - 3
SP  - 1
VL  - 11
DO  - 10.3390/ani11030844
ER  - 

@article{
author = "Núñez, Yolanda and Radović, Čedomir and Savić, Radomir and García-Casco, Juan M. and Čandek-Potokar, Marjeta and Benítez, Rita and Radojković, Dragan and Lukić, Miloš and Gogić, Marija and Muñoz, María and Fontanesi, Luca and Óvilo, Cristina",
year = "2021",
abstract = "This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.",
publisher = "MDPI AG",
journal = "Animals",
title = "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs",
pages = "24-1",
number = "3",
volume = "11",
doi = "10.3390/ani11030844"
}

Núñez, Y., Radović, Č., Savić, R., García-Casco, J. M., Čandek-Potokar, M., Benítez, R., Radojković, D., Lukić, M., Gogić, M., Muñoz, M., Fontanesi, L.,& Óvilo, C.. (2021). Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals
MDPI AG., 11(3), 1-24.
https://doi.org/10.3390/ani11030844

Núñez Y, Radović Č, Savić R, García-Casco JM, Čandek-Potokar M, Benítez R, Radojković D, Lukić M, Gogić M, Muñoz M, Fontanesi L, Óvilo C. Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals. 2021;11(3):1-24.
doi:10.3390/ani11030844 .

Núñez, Yolanda, Radović, Čedomir, Savić, Radomir, García-Casco, Juan M., Čandek-Potokar, Marjeta, Benítez, Rita, Radojković, Dragan, Lukić, Miloš, Gogić, Marija, Muñoz, María, Fontanesi, Luca, Óvilo, Cristina, "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs" in Animals, 11, no. 3 (2021):1-24,
https://doi.org/10.3390/ani11030844 . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Lukić, Miloš
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Nikolić, Milica
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5484
AB  - The objective of the present study was to investigate the susceptibility of maize hybrids to the natural occurrence of toxigenic fungal species, in particular toxigenic Aspergillus and Fusarium species, and mycotoxins (aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins B1, B2 and B3 (FBs)). Grain samples of six commercial maize hybrids (MAS 34.B - FAO 300, MAS 40.F, MAS 48.L, KWS Konfites and ZP 427 - FAO 400, and MAS 56.A - FAO 500) were collected at harvest in 2018. A total of seven fungal genera, Acremonium, Alternaria, Epicoccum, Fusarium, Nigrospora, Penicillium and Rhizopus, were identified of which only species from the genus Fusarium were present on maize grains of all hybrids tested. The incidence of Fusarium spp. was higher in the hybrids MAS 48.L (54.6%), and MAS 56.A (53.3%), compared to MAS 40.F (37.3%), KWS Konfites (28%), MAS 34.B (22.6%) and ZP 427 (12%) hybrids. Among the identified Fusarium species (F. graminearum, F. proliferatum, F. subglutinans and F. verticillioides), F. proliferatum was present in all hybrids, ranging from 9.3% (ZP 427) to 30.7% (MAS 48.L ), whereas F. subglutinans was present in two hybrids, MAS 40.F (16%) and MAS 56.A (9.3%). The incidence of F. graminearum ranged from 0% (KWS Konfites) to 9.3% (MAS 34.B), while the incidence of F. verticillioides ranged from 0% (MAS 34.B and ZP 427) to 21.3% (MAS 48.L). In the samples, Aspergillus species were not identified. The effect of maize hybrids was significant on the level of mycotoxins. MAS 34.B hybrid had a statistically significantly higher levels of AFB1 and DON than other hybrids. The FBs level was the highest in the hybrid MAS 34.B (1202 mg kg-1 ) and the lowest in the hybrid KWS Konfites (88.33 mg kg-1 ). However, the FBs level did not differ between hybrids MAS 34.B, MAS 40.F, and MAS 56.L, MAS 40.F, MAS 48.L, and MAS 56.A, and KWS Konfites and ZP 427. In all hybrids, AFB1, DON, and FBs levels were below the maximum permissible levels stipulated by the legislation of the European Union and the Republic of Serbia in unprocessed maize. The agro-ecological conditions in 2018 favored the development of Fusarium species on maize grains of the hybrids tested, especially fumonisin producing species.
AB  - Cilj rada je bio da se ispita osetljivost razliĉitih hibrida kukuruza na prirodnu pojavu toksigenih vrsta gljiva, posebno toksigenih Aspergillus i Fusarium vrsta, i mikotoksina (aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina B1, B2 i B3 (FBs)). Uzorci zrna šest komercijalnih hibrida kukuruza (MAS 34.B - FAO 300, MAS 40.F, MAS 48.L, KWS Konfites i ZP 427 - FAO 400 i i MAS 56.A - FAO 500) sakupljeni su u vreme berbe 2018. godine. Analizom mikobiota identifikovane su vrste iz sedam rodova, Acremonium, Alternaria, Epicoccum, Fusarium, Nigrospora, Penicillium i Rhizopus, od kojih su samo vrste iz roda Fusarium bile prisutne na zrnu kukuruza svih ispitivanih hibrida. Veća uĉestalost Fusarium spp. na zrnu kukuruza bila je kod hibrida MAS 48.L (54,6%) i MAS 56.A (53,3%) u poreĊenju sa hibridima MAS 40.F (37,3%), KWS Konfites (28%), MAS 34.B (22,6%) i ZP 427 (12%). MeĊu identifikovanim Fusarium vrstama (F. graminearum, F. proliferatum, F. subglutinans i F. verticillioides), vrsta F. proliferatum bila je prisutna kod svih ispitivanih hibrida u rangu od 9,3% (ZP 427) do 30,7% (MAS 48.L), dok je vrsta F. subglutinans bila prisutna kod dva hibrida MAS 40.F (16%) i MAS 56.A (9,3%). Uĉestalost F. graminearum je bila od 0% (KWS Konfites) do 9,3% (MAS 34.B), dok je uĉestalost F. verticillioides bila od 0% (MAS 34.B i ZP427) do 21,3% (MAS 48.L). U uzorcima zrna, Aspergillus vrste nisu bile identifikovane. Hibridi kukuruza statistiĉki su znaĉajno uticali na sadrţaj ispitivanih mikotoksina. Hibrid MAS 34.B imao je statistiĉki znaĉajno viši sadrţaj AFB1 i DON u odnosu na druge hibride. Sadrţaj FBs bio je najviši kod hibrida MAS 34.B (1202 mg kg-1 ), a najmanji kod hibrida KWS Konfites (88,33 mg kg-1 ). MeĊutim, sadrţaj FBs nije se razlikovao izmeĊu hibrida MAS 34.B, MAS 40.F i MAS 56.A, MAS 40.F, MAS 48.L i MAS 56.A i KWS Konfites i ZP 427. Kod svih ispitivanih hibrida, sadrţaji AFB1, DON i FBs bili su ispod maksimalno dozvoljenih koliĉina propisanih zakonskom regulativom Evropske Unije i Republike Srbije u nepreraĊenom kukuruzu. Agroekološki uslovi u 2018. godini pogodovali su razvoju Fusarium vrsta na zrnu kukuruza ispitivanih hibrida, i to posebno fumonizin producenata.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Natural toxigenic fungal and mycotoxin occurrence in maize hybrids
T1  - Prirodna pojava toksigenih gljiva i mikotoksina u hibridima kukuruza
EP  - 85
IS  - 1
SP  - 75
VL  - 36
DO  - 10.2298/BAH2001075K
ER  - 

@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Lukić, Miloš and Petrović, Tanja and Stanković, Slavica and Nikolić, Milica",
year = "2020",
abstract = "The objective of the present study was to investigate the susceptibility of maize hybrids to the natural occurrence of toxigenic fungal species, in particular toxigenic Aspergillus and Fusarium species, and mycotoxins (aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins B1, B2 and B3 (FBs)). Grain samples of six commercial maize hybrids (MAS 34.B - FAO 300, MAS 40.F, MAS 48.L, KWS Konfites and ZP 427 - FAO 400, and MAS 56.A - FAO 500) were collected at harvest in 2018. A total of seven fungal genera, Acremonium, Alternaria, Epicoccum, Fusarium, Nigrospora, Penicillium and Rhizopus, were identified of which only species from the genus Fusarium were present on maize grains of all hybrids tested. The incidence of Fusarium spp. was higher in the hybrids MAS 48.L (54.6%), and MAS 56.A (53.3%), compared to MAS 40.F (37.3%), KWS Konfites (28%), MAS 34.B (22.6%) and ZP 427 (12%) hybrids. Among the identified Fusarium species (F. graminearum, F. proliferatum, F. subglutinans and F. verticillioides), F. proliferatum was present in all hybrids, ranging from 9.3% (ZP 427) to 30.7% (MAS 48.L ), whereas F. subglutinans was present in two hybrids, MAS 40.F (16%) and MAS 56.A (9.3%). The incidence of F. graminearum ranged from 0% (KWS Konfites) to 9.3% (MAS 34.B), while the incidence of F. verticillioides ranged from 0% (MAS 34.B and ZP 427) to 21.3% (MAS 48.L). In the samples, Aspergillus species were not identified. The effect of maize hybrids was significant on the level of mycotoxins. MAS 34.B hybrid had a statistically significantly higher levels of AFB1 and DON than other hybrids. The FBs level was the highest in the hybrid MAS 34.B (1202 mg kg-1 ) and the lowest in the hybrid KWS Konfites (88.33 mg kg-1 ). However, the FBs level did not differ between hybrids MAS 34.B, MAS 40.F, and MAS 56.L, MAS 40.F, MAS 48.L, and MAS 56.A, and KWS Konfites and ZP 427. In all hybrids, AFB1, DON, and FBs levels were below the maximum permissible levels stipulated by the legislation of the European Union and the Republic of Serbia in unprocessed maize. The agro-ecological conditions in 2018 favored the development of Fusarium species on maize grains of the hybrids tested, especially fumonisin producing species., Cilj rada je bio da se ispita osetljivost razliĉitih hibrida kukuruza na prirodnu pojavu toksigenih vrsta gljiva, posebno toksigenih Aspergillus i Fusarium vrsta, i mikotoksina (aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina B1, B2 i B3 (FBs)). Uzorci zrna šest komercijalnih hibrida kukuruza (MAS 34.B - FAO 300, MAS 40.F, MAS 48.L, KWS Konfites i ZP 427 - FAO 400 i i MAS 56.A - FAO 500) sakupljeni su u vreme berbe 2018. godine. Analizom mikobiota identifikovane su vrste iz sedam rodova, Acremonium, Alternaria, Epicoccum, Fusarium, Nigrospora, Penicillium i Rhizopus, od kojih su samo vrste iz roda Fusarium bile prisutne na zrnu kukuruza svih ispitivanih hibrida. Veća uĉestalost Fusarium spp. na zrnu kukuruza bila je kod hibrida MAS 48.L (54,6%) i MAS 56.A (53,3%) u poreĊenju sa hibridima MAS 40.F (37,3%), KWS Konfites (28%), MAS 34.B (22,6%) i ZP 427 (12%). MeĊu identifikovanim Fusarium vrstama (F. graminearum, F. proliferatum, F. subglutinans i F. verticillioides), vrsta F. proliferatum bila je prisutna kod svih ispitivanih hibrida u rangu od 9,3% (ZP 427) do 30,7% (MAS 48.L), dok je vrsta F. subglutinans bila prisutna kod dva hibrida MAS 40.F (16%) i MAS 56.A (9,3%). Uĉestalost F. graminearum je bila od 0% (KWS Konfites) do 9,3% (MAS 34.B), dok je uĉestalost F. verticillioides bila od 0% (MAS 34.B i ZP427) do 21,3% (MAS 48.L). U uzorcima zrna, Aspergillus vrste nisu bile identifikovane. Hibridi kukuruza statistiĉki su znaĉajno uticali na sadrţaj ispitivanih mikotoksina. Hibrid MAS 34.B imao je statistiĉki znaĉajno viši sadrţaj AFB1 i DON u odnosu na druge hibride. Sadrţaj FBs bio je najviši kod hibrida MAS 34.B (1202 mg kg-1 ), a najmanji kod hibrida KWS Konfites (88,33 mg kg-1 ). MeĊutim, sadrţaj FBs nije se razlikovao izmeĊu hibrida MAS 34.B, MAS 40.F i MAS 56.A, MAS 40.F, MAS 48.L i MAS 56.A i KWS Konfites i ZP 427. Kod svih ispitivanih hibrida, sadrţaji AFB1, DON i FBs bili su ispod maksimalno dozvoljenih koliĉina propisanih zakonskom regulativom Evropske Unije i Republike Srbije u nepreraĊenom kukuruzu. Agroekološki uslovi u 2018. godini pogodovali su razvoju Fusarium vrsta na zrnu kukuruza ispitivanih hibrida, i to posebno fumonizin producenata.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Natural toxigenic fungal and mycotoxin occurrence in maize hybrids, Prirodna pojava toksigenih gljiva i mikotoksina u hibridima kukuruza",
pages = "85-75",
number = "1",
volume = "36",
doi = "10.2298/BAH2001075K"
}

Krnjaja, V., Mandić, V., Bijelić, Z., Lukić, M., Petrović, T., Stanković, S.,& Nikolić, M.. (2020). Natural toxigenic fungal and mycotoxin occurrence in maize hybrids. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 36(1), 75-85.
https://doi.org/10.2298/BAH2001075K

Krnjaja V, Mandić V, Bijelić Z, Lukić M, Petrović T, Stanković S, Nikolić M. Natural toxigenic fungal and mycotoxin occurrence in maize hybrids. in Biotechnology in Animal Husbandry. 2020;36(1):75-85.
doi:10.2298/BAH2001075K .

Krnjaja, Vesna, Mandić, Violeta, Bijelić, Zorica, Lukić, Miloš, Petrović, Tanja, Stanković, Slavica, Nikolić, Milica, "Natural toxigenic fungal and mycotoxin occurrence in maize hybrids" in Biotechnology in Animal Husbandry, 36, no. 1 (2020):75-85,
https://doi.org/10.2298/BAH2001075K . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5212
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
T1  - Mikobiota i aflatoksin B1 u hrani za živinu
EP  - 69
IS  - 1
SP  - 61
VL  - 35
DO  - 10.2298/BAH1901061K
ER  - 

@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
pages = "69-61",
number = "1",
volume = "35",
doi = "10.2298/BAH1901061K"
}

Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z.. (2019). Mycobiota and aflatoxin B1 in poultry feeds. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K

Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mycobiota and aflatoxin B1 in poultry feeds. in Biotechnology in Animal Husbandry. 2019;35(1):61-69.
doi:10.2298/BAH1901061K .

Krnjaja, Vesna, Petrović, Tanja, Stanković, Slavica, Lukić, Miloš, Škrbić, Zdenka, Mandić, Violeta, Bijelić, Zorica, "Mycobiota and aflatoxin B1 in poultry feeds" in Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K . .

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4862
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
EP  - 249
IS  - 2
SP  - 239
VL  - 34
DO  - 10.2298/bah1802239K
ER  - 

@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
pages = "249-239",
number = "2",
volume = "34",
doi = "10.2298/bah1802239K"
}

Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V.. (2018). Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K

Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia. in Biotechnology in Animal Husbandry. 2018;34(2):239-249.
doi:10.2298/bah1802239K .

Krnjaja, Vesna, Stanković, Slavica, Lukić, Miloš, Mićić, Nenad, Petrović, Tanja, Bijelić, Zorica, Mandić, Violeta, "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia" in Biotechnology in Animal Husbandry, 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K . .

TY  - JOUR
AU  - Žujović, Miroslav
AU  - Tomić, Zorica P.
AU  - Cmiljanić, Ratimir
AU  - Vlahović, Milica
AU  - Josipović, Slavko
AU  - Lukić, Miloš
AU  - Strsoglavec, Stela
AU  - Trenkovski, Snežana
AU  - Nešić, Zorica
AU  - Jakić-Dimić, Dobrila
AU  - Pavlović, Ivan
AU  - Ivanović, Snežana
AU  - Riznić, Ljiljana
AU  - Mekić, Cvijan
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/564
AB  - Project research includes investigation of environmental conditions in which sheep are raised, also breed structure in region of Stara planina mountain and genetic potential of sheep population for production of high quality lamb meat. Heterosis effect is also investigated, and new created genotypes, beside improved performance in regard to gain, feed conversion and dressing percentage, will have as consequence improvement of the quality and quantity of carcass and meat of lambs. New created genotypes can be successfully raised in hilly, hilly-mountainous and plain regions. Existing technological solutions in regard to housing of sheep and lambs will be innovated by introduction of several variants and systems of nutrition which would include maximum use of roughage - pasture, hey and silage for grown heads of livestock, with addition of adequate quantities of concentrated feeds, also solutions introducing the highest degree of rationalization in production of high quality lamb meat will be selected. Floral composition and nutritive value of grass mass at our disposal as well as grain feeds are to be analyzed. Based on forage plant base at our disposal and quality of feeds the concrete programme of nutrition of lambs according to fattening phase will be applied. During fattening, effect on nutrition on production performance (gain of body mass and feed conversion) will be monitored. Reproductive traits of sheep - dams and ram sires will be analyzed, also planned health protection of animals carried out. Also economical efficiency of organized lamb breeding for production of high quality lamb meat analyzed. Most favorable anatomical categories for packaging will be determined as well as physical, chemical and sensory parameters of the quality of lamb meat defined. Special attention will be directed towards the quantity and quality of obtained lamb carcasses and compared with EU standards.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Study of the project 'production of Lamb meat'
T1  - Studija projekta 'proizvodnja jagnjećeg mesa'
EP  - 90
IS  - 1-2
SP  - 83
VL  - 19
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_564
ER  - 

@article{
author = "Žujović, Miroslav and Tomić, Zorica P. and Cmiljanić, Ratimir and Vlahović, Milica and Josipović, Slavko and Lukić, Miloš and Strsoglavec, Stela and Trenkovski, Snežana and Nešić, Zorica and Jakić-Dimić, Dobrila and Pavlović, Ivan and Ivanović, Snežana and Riznić, Ljiljana and Mekić, Cvijan",
year = "2003",
abstract = "Project research includes investigation of environmental conditions in which sheep are raised, also breed structure in region of Stara planina mountain and genetic potential of sheep population for production of high quality lamb meat. Heterosis effect is also investigated, and new created genotypes, beside improved performance in regard to gain, feed conversion and dressing percentage, will have as consequence improvement of the quality and quantity of carcass and meat of lambs. New created genotypes can be successfully raised in hilly, hilly-mountainous and plain regions. Existing technological solutions in regard to housing of sheep and lambs will be innovated by introduction of several variants and systems of nutrition which would include maximum use of roughage - pasture, hey and silage for grown heads of livestock, with addition of adequate quantities of concentrated feeds, also solutions introducing the highest degree of rationalization in production of high quality lamb meat will be selected. Floral composition and nutritive value of grass mass at our disposal as well as grain feeds are to be analyzed. Based on forage plant base at our disposal and quality of feeds the concrete programme of nutrition of lambs according to fattening phase will be applied. During fattening, effect on nutrition on production performance (gain of body mass and feed conversion) will be monitored. Reproductive traits of sheep - dams and ram sires will be analyzed, also planned health protection of animals carried out. Also economical efficiency of organized lamb breeding for production of high quality lamb meat analyzed. Most favorable anatomical categories for packaging will be determined as well as physical, chemical and sensory parameters of the quality of lamb meat defined. Special attention will be directed towards the quantity and quality of obtained lamb carcasses and compared with EU standards.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Study of the project 'production of Lamb meat', Studija projekta 'proizvodnja jagnjećeg mesa'",
pages = "90-83",
number = "1-2",
volume = "19",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_564"
}

Žujović, M., Tomić, Z. P., Cmiljanić, R., Vlahović, M., Josipović, S., Lukić, M., Strsoglavec, S., Trenkovski, S., Nešić, Z., Jakić-Dimić, D., Pavlović, I., Ivanović, S., Riznić, L.,& Mekić, C.. (2003). Study of the project 'production of Lamb meat'. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 19(1-2), 83-90.
https://hdl.handle.net/21.15107/rcub_agrospace_564

Žujović M, Tomić ZP, Cmiljanić R, Vlahović M, Josipović S, Lukić M, Strsoglavec S, Trenkovski S, Nešić Z, Jakić-Dimić D, Pavlović I, Ivanović S, Riznić L, Mekić C. Study of the project 'production of Lamb meat'. in Biotechnology in Animal Husbandry. 2003;19(1-2):83-90.
https://hdl.handle.net/21.15107/rcub_agrospace_564 .

Žujović, Miroslav, Tomić, Zorica P., Cmiljanić, Ratimir, Vlahović, Milica, Josipović, Slavko, Lukić, Miloš, Strsoglavec, Stela, Trenkovski, Snežana, Nešić, Zorica, Jakić-Dimić, Dobrila, Pavlović, Ivan, Ivanović, Snežana, Riznić, Ljiljana, Mekić, Cvijan, "Study of the project 'production of Lamb meat'" in Biotechnology in Animal Husbandry, 19, no. 1-2 (2003):83-90,
https://hdl.handle.net/21.15107/rcub_agrospace_564 .