TY  - JOUR
AU  - Jovanović, Gordana D.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4021
AB  - The antibacterial activity of chitosan coatings prepared with acetic or lactic acid, as well as of composite chitosan-gelatin films prepared with essential oils, was evaluated in fresh shredded black radish samples inoculated with Listeria monocytogenes ATCC 19115 and L. monocytogenes ATCC 19112 during seven days of storage at 4 degrees C. The chitosan coating prepared with acetic acid showed the most effective antibacterial activity. All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of L. monocytogenes on black radish, although a higher inhibition of pathogens was achieved at higher concentrations of chitosan. The antimicrobial effect of chitosan films was even more pronounced with the addition of essential oils. Chitosan-gelatin films with thyme essential oils showed the most effective antimicrobial activity. A reduction of 2.4 log(10) CFU/g for L. monocytogenes ATCC 19115 and 2.1 log(10) CFU/g for L. monocytogenes ATCC 19112 was achieved in the presence of 1% chitosan film containing 0.2% of thyme essential oil after 24 h of storage.
PB  - Asociacion Argentina Microbiologia, Buenos Aires
T2  - Revista Argentina de Microbiologia
T1  - Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish
EP  - 136
IS  - 2
SP  - 128
VL  - 48
DO  - 10.1016/j.ram.2016.02.003
ER  - 

@article{
author = "Jovanović, Gordana D. and Klaus, Anita and Nikšić, Miomir",
year = "2016",
abstract = "The antibacterial activity of chitosan coatings prepared with acetic or lactic acid, as well as of composite chitosan-gelatin films prepared with essential oils, was evaluated in fresh shredded black radish samples inoculated with Listeria monocytogenes ATCC 19115 and L. monocytogenes ATCC 19112 during seven days of storage at 4 degrees C. The chitosan coating prepared with acetic acid showed the most effective antibacterial activity. All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of L. monocytogenes on black radish, although a higher inhibition of pathogens was achieved at higher concentrations of chitosan. The antimicrobial effect of chitosan films was even more pronounced with the addition of essential oils. Chitosan-gelatin films with thyme essential oils showed the most effective antimicrobial activity. A reduction of 2.4 log(10) CFU/g for L. monocytogenes ATCC 19115 and 2.1 log(10) CFU/g for L. monocytogenes ATCC 19112 was achieved in the presence of 1% chitosan film containing 0.2% of thyme essential oil after 24 h of storage.",
publisher = "Asociacion Argentina Microbiologia, Buenos Aires",
journal = "Revista Argentina de Microbiologia",
title = "Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish",
pages = "136-128",
number = "2",
volume = "48",
doi = "10.1016/j.ram.2016.02.003"
}

Jovanović, G. D., Klaus, A.,& Nikšić, M.. (2016). Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish. in Revista Argentina de Microbiologia
Asociacion Argentina Microbiologia, Buenos Aires., 48(2), 128-136.
https://doi.org/10.1016/j.ram.2016.02.003

Jovanović GD, Klaus A, Nikšić M. Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish. in Revista Argentina de Microbiologia. 2016;48(2):128-136.
doi:10.1016/j.ram.2016.02.003 .

Jovanović, Gordana D., Klaus, Anita, Nikšić, Miomir, "Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish" in Revista Argentina de Microbiologia, 48, no. 2 (2016):128-136,
https://doi.org/10.1016/j.ram.2016.02.003 . .

TY  - JOUR
AU  - Jovanović, Gordana D.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4085
AB  - BACKGROUND: The highest incidence of listeriosis, due to consumption of ready-to-eat foods and fresh, shredded, minimally processed vegetables, occurs among pregnant women and the elderly. In order to reduce the prevalence of listeriosis among consumers, better protective measures are recommended. Chitosan films, with or without added essential oils, represent a modern, safe method of preserving the quality of such vegetables and significantly reducing the incidence of Listeria monocytogenes in these foods. Objectives: The present study was conducted to evaluate the antimicrobial properties of composite chitosan-gelatin films with and without essential oils against two strains of L. monocytogenes, ATCC 19115 and ATCC 19112, in fresh shredded cabbage. Methods: Shredded cabbage was inoculated with L. monocytogenes and packed between two layers of the chitosan composite film, then placed in Petri dishes. The prepared samples were stored at 4 degrees C then analyzed for total viable count on PALCAM agar while incubated at 37 degrees C, every 24 hours for 7 days. Results: Average L. monocytogenes content ranged from 4.2 - 5.4 log CFU/g, reaching values of 7.2 - 8.6 log CFU/g in samples of untreated cabbage. A complete reduction of L. monocytogenes ATCC 19115 on cabbage was achieved after 120 hours in the presence of 0.5% chitosan film, whereas reduction of L. monocytogenes ATCC 19112 was achieved after 144 hours. In the presence of 1% chitosan film, the bacteria withered more quickly and complete reduction of both species of L. monocytogenes was achieved after 96 hours. Conclusions: All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of both strains of L. monocytogenes on cabbage. The best effect was achieved with a 1% chitosan concentration. The addition of essential oils increased the antimicrobial activity of all tested films.
PB  - Ahvaz Jundishapur Univ Med Sci, Ahvaz
T2  - Jundishapur Journal of Microbiology
T1  - Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage
IS  - 9
VL  - 9
DO  - 10.5812/jjm.34804
ER  - 

@article{
author = "Jovanović, Gordana D. and Klaus, Anita and Nikšić, Miomir",
year = "2016",
abstract = "BACKGROUND: The highest incidence of listeriosis, due to consumption of ready-to-eat foods and fresh, shredded, minimally processed vegetables, occurs among pregnant women and the elderly. In order to reduce the prevalence of listeriosis among consumers, better protective measures are recommended. Chitosan films, with or without added essential oils, represent a modern, safe method of preserving the quality of such vegetables and significantly reducing the incidence of Listeria monocytogenes in these foods. Objectives: The present study was conducted to evaluate the antimicrobial properties of composite chitosan-gelatin films with and without essential oils against two strains of L. monocytogenes, ATCC 19115 and ATCC 19112, in fresh shredded cabbage. Methods: Shredded cabbage was inoculated with L. monocytogenes and packed between two layers of the chitosan composite film, then placed in Petri dishes. The prepared samples were stored at 4 degrees C then analyzed for total viable count on PALCAM agar while incubated at 37 degrees C, every 24 hours for 7 days. Results: Average L. monocytogenes content ranged from 4.2 - 5.4 log CFU/g, reaching values of 7.2 - 8.6 log CFU/g in samples of untreated cabbage. A complete reduction of L. monocytogenes ATCC 19115 on cabbage was achieved after 120 hours in the presence of 0.5% chitosan film, whereas reduction of L. monocytogenes ATCC 19112 was achieved after 144 hours. In the presence of 1% chitosan film, the bacteria withered more quickly and complete reduction of both species of L. monocytogenes was achieved after 96 hours. Conclusions: All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of both strains of L. monocytogenes on cabbage. The best effect was achieved with a 1% chitosan concentration. The addition of essential oils increased the antimicrobial activity of all tested films.",
publisher = "Ahvaz Jundishapur Univ Med Sci, Ahvaz",
journal = "Jundishapur Journal of Microbiology",
title = "Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage",
number = "9",
volume = "9",
doi = "10.5812/jjm.34804"
}

Jovanović, G. D., Klaus, A.,& Nikšić, M.. (2016). Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage. in Jundishapur Journal of Microbiology
Ahvaz Jundishapur Univ Med Sci, Ahvaz., 9(9).
https://doi.org/10.5812/jjm.34804

Jovanović GD, Klaus A, Nikšić M. Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage. in Jundishapur Journal of Microbiology. 2016;9(9).
doi:10.5812/jjm.34804 .

Jovanović, Gordana D., Klaus, Anita, Nikšić, Miomir, "Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage" in Jundishapur Journal of Microbiology, 9, no. 9 (2016),
https://doi.org/10.5812/jjm.34804 . .

TY  - JOUR
AU  - Jovanović, Gordana D.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4286
AB  - The objective of this study was to investigate the antimicrobial effects of chitosan gelatin films with or without added essential oils against Listeria monocytogenes on fresh shredded cabbage, carrot and black radish. Samples of fresh shredded vegetables were inoculated with L. monocytogenes and then stored between two layers of edible chitosan films at various concentrations, with or without added essential oils. The inoculated, film treated samples were stored at 4 °C. Samples were taken every day,during six days, for enumeration of surviving L. monocytogenes by plating on appropriate media. Compared to control,1% chitosan film and 0.5% film-induced to 4.7 and 2.9 log CFU/g reductions on shredded cabbage 3rd day respectively. After 3 days, films with 1% chitosan reduced L. monocytogenes population by 3 log CFU/g on carrots. Antimicrobial activity of chitosan film increases with increasing concentrations of applied chitosan, where 1% chitosan film has the highest antimicrobial activity compared to 0.5% chitosan film. Nevertheless, chitosan films were more effective on black radish than on cabbage and carrot. Addition of thyme essential oils, significantly improves the activity of the antimicrobial film and reduce the time required for the inhibition of bacteria in vegetables. Also, changes of color, taste, aroma and chemical composition of non-treated and treated samples were determined. The application of chitosan films exerts a significant influence on the pH changes and content of soluble solids of stored vegetables. Chitosan films improved sample appearance, since they diminished the occurrence of the color changes during storage. The results of this experiment showed that the use of chitosan films is a viable alternative in controlling the microorganisms present in minimally processed vegetables, improving its sensory quality. The advantages of edible films were the simple manufacturing process, biodegradability and usage of natural and safe compounds.
PB  - Društvo za ishranu Srbije, Beograd
T2  - Hrana i ishrana
T1  - Application of chitosan films in the quality control of fresh shredded vegetables
EP  - 36
IS  - 1
SP  - 29
VL  - 57
DO  - 10.5937/HraIsh1601029J
ER  - 

@article{
author = "Jovanović, Gordana D. and Klaus, Anita and Nikšić, Miomir",
year = "2016",
abstract = "The objective of this study was to investigate the antimicrobial effects of chitosan gelatin films with or without added essential oils against Listeria monocytogenes on fresh shredded cabbage, carrot and black radish. Samples of fresh shredded vegetables were inoculated with L. monocytogenes and then stored between two layers of edible chitosan films at various concentrations, with or without added essential oils. The inoculated, film treated samples were stored at 4 °C. Samples were taken every day,during six days, for enumeration of surviving L. monocytogenes by plating on appropriate media. Compared to control,1% chitosan film and 0.5% film-induced to 4.7 and 2.9 log CFU/g reductions on shredded cabbage 3rd day respectively. After 3 days, films with 1% chitosan reduced L. monocytogenes population by 3 log CFU/g on carrots. Antimicrobial activity of chitosan film increases with increasing concentrations of applied chitosan, where 1% chitosan film has the highest antimicrobial activity compared to 0.5% chitosan film. Nevertheless, chitosan films were more effective on black radish than on cabbage and carrot. Addition of thyme essential oils, significantly improves the activity of the antimicrobial film and reduce the time required for the inhibition of bacteria in vegetables. Also, changes of color, taste, aroma and chemical composition of non-treated and treated samples were determined. The application of chitosan films exerts a significant influence on the pH changes and content of soluble solids of stored vegetables. Chitosan films improved sample appearance, since they diminished the occurrence of the color changes during storage. The results of this experiment showed that the use of chitosan films is a viable alternative in controlling the microorganisms present in minimally processed vegetables, improving its sensory quality. The advantages of edible films were the simple manufacturing process, biodegradability and usage of natural and safe compounds.",
publisher = "Društvo za ishranu Srbije, Beograd",
journal = "Hrana i ishrana",
title = "Application of chitosan films in the quality control of fresh shredded vegetables",
pages = "36-29",
number = "1",
volume = "57",
doi = "10.5937/HraIsh1601029J"
}

Jovanović, G. D., Klaus, A.,& Nikšić, M.. (2016). Application of chitosan films in the quality control of fresh shredded vegetables. in Hrana i ishrana
Društvo za ishranu Srbije, Beograd., 57(1), 29-36.
https://doi.org/10.5937/HraIsh1601029J

Jovanović GD, Klaus A, Nikšić M. Application of chitosan films in the quality control of fresh shredded vegetables. in Hrana i ishrana. 2016;57(1):29-36.
doi:10.5937/HraIsh1601029J .

Jovanović, Gordana D., Klaus, Anita, Nikšić, Miomir, "Application of chitosan films in the quality control of fresh shredded vegetables" in Hrana i ishrana, 57, no. 1 (2016):29-36,
https://doi.org/10.5937/HraIsh1601029J . .