Radić, Danka

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  • Radić, Danka (13)

Author's Bibliography

Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens

Vunduk, Jovana; Klaus, Anita; Lazić, Vesna; Kozarski, Maja; Radić, Danka; Šovljanski, Olja; Pezo, Lato

(2023)

TY  - JOUR
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Lazić, Vesna
AU  - Kozarski, Maja
AU  - Radić, Danka
AU  - Šovljanski, Olja
AU  - Pezo, Lato
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6323
AB  - The problem of microbial biofilms has come to the fore alongside food, pharmaceutical, and healthcare industrialization. The development of new antibiofilm products has become urgent, but it includes bioprospecting and is time and money-consuming. Contemporary efforts are directed at the pursuit of effective compounds of natural origin, also known as “green” agents. Mushrooms appear to be a possible new source of antibiofilm compounds, as has been demonstrated recently. The existing modeling methods are directed toward predicting bacterial biofilm formation, not in the presence of antibiofilm materials. Moreover, the modeling is almost exclusively targeted at biofilms in healthcare, while modeling related to the food industry remains under-researched. The present study applied an Artificial Neural Network (ANN) model to analyze the anti-adhesion and anti-biofilm-forming effects of 40 extracts from 20 mushroom species against two very important food-borne bacterial species for food and food-related industries—Listeria monocytogenes and Salmonella enteritidis. The models developed in this study exhibited high prediction quality, as indicated by high r2 values during the training cycle. The best fit between the modeled and measured values was observed for the inhibition of adhesion. This study provides a valuable contribution to the field, supporting industrial settings during the initial stage of biofilm formation, when these communities are the most vulnerable, and promoting innovative and improved safety management. © 2023 by the authors.
T2  - Antibiotics
T2  - Antibiotics
T1  - Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens
IS  - 3
VL  - 12
DO  - 10.3390/antibiotics12030627
ER  - 
@article{
author = "Vunduk, Jovana and Klaus, Anita and Lazić, Vesna and Kozarski, Maja and Radić, Danka and Šovljanski, Olja and Pezo, Lato",
year = "2023",
abstract = "The problem of microbial biofilms has come to the fore alongside food, pharmaceutical, and healthcare industrialization. The development of new antibiofilm products has become urgent, but it includes bioprospecting and is time and money-consuming. Contemporary efforts are directed at the pursuit of effective compounds of natural origin, also known as “green” agents. Mushrooms appear to be a possible new source of antibiofilm compounds, as has been demonstrated recently. The existing modeling methods are directed toward predicting bacterial biofilm formation, not in the presence of antibiofilm materials. Moreover, the modeling is almost exclusively targeted at biofilms in healthcare, while modeling related to the food industry remains under-researched. The present study applied an Artificial Neural Network (ANN) model to analyze the anti-adhesion and anti-biofilm-forming effects of 40 extracts from 20 mushroom species against two very important food-borne bacterial species for food and food-related industries—Listeria monocytogenes and Salmonella enteritidis. The models developed in this study exhibited high prediction quality, as indicated by high r2 values during the training cycle. The best fit between the modeled and measured values was observed for the inhibition of adhesion. This study provides a valuable contribution to the field, supporting industrial settings during the initial stage of biofilm formation, when these communities are the most vulnerable, and promoting innovative and improved safety management. © 2023 by the authors.",
journal = "Antibiotics, Antibiotics",
title = "Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens",
number = "3",
volume = "12",
doi = "10.3390/antibiotics12030627"
}
Vunduk, J., Klaus, A., Lazić, V., Kozarski, M., Radić, D., Šovljanski, O.,& Pezo, L.. (2023). Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens. in Antibiotics, 12(3).
https://doi.org/10.3390/antibiotics12030627
Vunduk J, Klaus A, Lazić V, Kozarski M, Radić D, Šovljanski O, Pezo L. Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens. in Antibiotics. 2023;12(3).
doi:10.3390/antibiotics12030627 .
Vunduk, Jovana, Klaus, Anita, Lazić, Vesna, Kozarski, Maja, Radić, Danka, Šovljanski, Olja, Pezo, Lato, "Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens" in Antibiotics, 12, no. 3 (2023),
https://doi.org/10.3390/antibiotics12030627 . .
3

Bacterial inoculation: A tool for red clover growth promotion in polluted soil

Karličić, Vera; Radić, Danka; Jovičić-Petrović, Jelena; Raičević, Vera

(Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd, 2020)

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5474
AB  - Red clover (Trifolium pratense L.) seeds were inoculated with several plant growth-promoting bacteria (PGPB) and sown in the substrate contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organometallic derivatives of tin (OT). The aim was to determine if selected PGPB strains can promote the growth of red clover in the substrate contaminated with several organic pollutants. The influence of bacteria on red clover growth (height, root length and biomass) was monitored during the three-month experimental period. The most significant improvements of seedling height were noted in the treatment with Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. Root growth was positively affected by Serratia liquefaciens Z-I ARV. The same isolates significantly affected biomass production. Those isolates caused total biomass increases of 70%, 48% and 33% compared to control. Bacterial strains used in this study were already confirmed as PGPB by biochemical testing, as well as by an in vivo test of mixed inoculums on several woody plants grown in the coal-mine overburden site. This work is the first-time record on their individual effects on one plant species. Obtained results confirmed that inoculation with several PGPB strains can enhance red clover growth in polluted soil.
AB  - Seme crvene deteline (Trifolium pratense L.), inokulisno sa nekoliko bakterija stimulatora biljnog rasta (PGPB), posejano je u supstrat kontaminiran policikličnim aromatičnim ugljovodonicima (PAHs), polihlorovanim bifenilima (PCBs) i organometalnim derivatima kalaja (OT). Cilj je bio da se utvrdi da li selektovane PGPB mogu promovisati rast crvene deteline u supstratu kontaminiranom sa nekoliko organskih zagađujućih materija. Uticaj bakterija na rast crvene deteline (visina, dužina korena i biomasa) praćen je tri meseca. Najveća visina je zabeležena kod biljaka inokulisanih sa Bacillus amyloliquefaciens D5 ARV i Pseudomonas putida P1 ARV. Rast korena je stimulisan od strane Serratia liquefaciens Z-I ARV. Ovi izolati su značajno uticali i na produkciju biomase. Ukupna biomasa dobijena tokom celog ogleda je za 70%, 48% i 33% veća u odnosu na kontrolu. Bakterijski sojevi korišćeni u ovoj studiji su prethodno potvrđeni kao PGPB kroz biohemijske i in vivo testove mešanog inokuluma na nekoliko drvenastih vrsta gajenih u jalovini. Ovaj rad prvi put beleži njihove pojedinačne efekte na jednu biljnu vrstu. Dobijeni rezultati potvrđuju da inokulacija sa nekoliko PGPB sojeva može ubrzati rast crvene deteline u zagađenom zemljištu.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Bacterial inoculation: A tool for red clover growth promotion in polluted soil
T1  - Bakterijska inokulacija - postupak za stimulaciju rasta crvene deteline gajene u zagađenom zemljištu
EP  - 174
IS  - 2
SP  - 163
VL  - 65
DO  - 10.2298/JAS2002163K
ER  - 
@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Raičević, Vera",
year = "2020",
abstract = "Red clover (Trifolium pratense L.) seeds were inoculated with several plant growth-promoting bacteria (PGPB) and sown in the substrate contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organometallic derivatives of tin (OT). The aim was to determine if selected PGPB strains can promote the growth of red clover in the substrate contaminated with several organic pollutants. The influence of bacteria on red clover growth (height, root length and biomass) was monitored during the three-month experimental period. The most significant improvements of seedling height were noted in the treatment with Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. Root growth was positively affected by Serratia liquefaciens Z-I ARV. The same isolates significantly affected biomass production. Those isolates caused total biomass increases of 70%, 48% and 33% compared to control. Bacterial strains used in this study were already confirmed as PGPB by biochemical testing, as well as by an in vivo test of mixed inoculums on several woody plants grown in the coal-mine overburden site. This work is the first-time record on their individual effects on one plant species. Obtained results confirmed that inoculation with several PGPB strains can enhance red clover growth in polluted soil., Seme crvene deteline (Trifolium pratense L.), inokulisno sa nekoliko bakterija stimulatora biljnog rasta (PGPB), posejano je u supstrat kontaminiran policikličnim aromatičnim ugljovodonicima (PAHs), polihlorovanim bifenilima (PCBs) i organometalnim derivatima kalaja (OT). Cilj je bio da se utvrdi da li selektovane PGPB mogu promovisati rast crvene deteline u supstratu kontaminiranom sa nekoliko organskih zagađujućih materija. Uticaj bakterija na rast crvene deteline (visina, dužina korena i biomasa) praćen je tri meseca. Najveća visina je zabeležena kod biljaka inokulisanih sa Bacillus amyloliquefaciens D5 ARV i Pseudomonas putida P1 ARV. Rast korena je stimulisan od strane Serratia liquefaciens Z-I ARV. Ovi izolati su značajno uticali i na produkciju biomase. Ukupna biomasa dobijena tokom celog ogleda je za 70%, 48% i 33% veća u odnosu na kontrolu. Bakterijski sojevi korišćeni u ovoj studiji su prethodno potvrđeni kao PGPB kroz biohemijske i in vivo testove mešanog inokuluma na nekoliko drvenastih vrsta gajenih u jalovini. Ovaj rad prvi put beleži njihove pojedinačne efekte na jednu biljnu vrstu. Dobijeni rezultati potvrđuju da inokulacija sa nekoliko PGPB sojeva može ubrzati rast crvene deteline u zagađenom zemljištu.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Bacterial inoculation: A tool for red clover growth promotion in polluted soil, Bakterijska inokulacija - postupak za stimulaciju rasta crvene deteline gajene u zagađenom zemljištu",
pages = "174-163",
number = "2",
volume = "65",
doi = "10.2298/JAS2002163K"
}
Karličić, V., Radić, D., Jovičić-Petrović, J.,& Raičević, V.. (2020). Bacterial inoculation: A tool for red clover growth promotion in polluted soil. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 65(2), 163-174.
https://doi.org/10.2298/JAS2002163K
Karličić V, Radić D, Jovičić-Petrović J, Raičević V. Bacterial inoculation: A tool for red clover growth promotion in polluted soil. in Journal of Agricultural Sciences (Belgrade). 2020;65(2):163-174.
doi:10.2298/JAS2002163K .
Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Raičević, Vera, "Bacterial inoculation: A tool for red clover growth promotion in polluted soil" in Journal of Agricultural Sciences (Belgrade), 65, no. 2 (2020):163-174,
https://doi.org/10.2298/JAS2002163K . .

Porous cordierite-supported polyethyleneimine composites for nickel(II) and cadmium(II) ions removal

Obradović, Nina; Rusmirović, Jelena D.; Filipović, Suzana; Kosanović, Darko; Marinković, Aleksandar; Radić, Danka; Pavlović, Vladimir

(Desalination Publ, Hopkinton, 2020)

TY  - JOUR
AU  - Obradović, Nina
AU  - Rusmirović, Jelena D.
AU  - Filipović, Suzana
AU  - Kosanović, Darko
AU  - Marinković, Aleksandar
AU  - Radić, Danka
AU  - Pavlović, Vladimir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5248
AB  - Industrial/technological growth is directly connected with environmental pollution, but its influence can be minimized through pollution abatement approaches such as the treatment of industrial wastewater. In this study, novel porous amine-functionalized silicate minerals, specifically, cordierite was investigated for the removal of toxic heavy metals from industrial wastewaters. Cordierite supports were synthesized by mixing MgO, Al2O3, and SiO2 powders in 2:2:5 molar ratios, and mechanically activated via ball milling in ethanol for 10, 40, or 80 min. Pellets were sintered by heating in air at 20 degrees C min(-1) to 1,350 degrees C, for 2 h. Porous supports were produced by coarsely crushing the sintered pellets and mixing the crushed and sieved cordierite powder with 20 wt.% of a pore-forming agent, either nanocellulose or yeast. The resulting pellets were sintered by heating at 5 degrees C min(-1) to 700 degrees C in air. The synthetic cordierite support was modified by treatment in poly-ethylenimine. Activated supports were then tested for the removal of Ni2+ and Cd2+ ions. The phase composition of the cordierite supports was analyzed by the X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy. Analysis of adsorption isotherms, kinetics, and thermodynamic parameters indicated that adsorption was a spontaneous, endothermic process with a maximum adsorption capacity of 36 mg g(-1) for Cd2+ and 43 mg g(-1) for Ni2+. This work has shed light on the mechanism of heavy metal removal from the aquatic medium using the novel hybrid functionalized cordierite-based ceramic.
PB  - Desalination Publ, Hopkinton
T2  - Desalination and Water Treatment
T1  - Porous cordierite-supported polyethyleneimine composites for nickel(II) and cadmium(II) ions removal
EP  - 296
SP  - 283
VL  - 192
DO  - 10.5004/dwt.2020.25736
ER  - 
@article{
author = "Obradović, Nina and Rusmirović, Jelena D. and Filipović, Suzana and Kosanović, Darko and Marinković, Aleksandar and Radić, Danka and Pavlović, Vladimir",
year = "2020",
abstract = "Industrial/technological growth is directly connected with environmental pollution, but its influence can be minimized through pollution abatement approaches such as the treatment of industrial wastewater. In this study, novel porous amine-functionalized silicate minerals, specifically, cordierite was investigated for the removal of toxic heavy metals from industrial wastewaters. Cordierite supports were synthesized by mixing MgO, Al2O3, and SiO2 powders in 2:2:5 molar ratios, and mechanically activated via ball milling in ethanol for 10, 40, or 80 min. Pellets were sintered by heating in air at 20 degrees C min(-1) to 1,350 degrees C, for 2 h. Porous supports were produced by coarsely crushing the sintered pellets and mixing the crushed and sieved cordierite powder with 20 wt.% of a pore-forming agent, either nanocellulose or yeast. The resulting pellets were sintered by heating at 5 degrees C min(-1) to 700 degrees C in air. The synthetic cordierite support was modified by treatment in poly-ethylenimine. Activated supports were then tested for the removal of Ni2+ and Cd2+ ions. The phase composition of the cordierite supports was analyzed by the X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy. Analysis of adsorption isotherms, kinetics, and thermodynamic parameters indicated that adsorption was a spontaneous, endothermic process with a maximum adsorption capacity of 36 mg g(-1) for Cd2+ and 43 mg g(-1) for Ni2+. This work has shed light on the mechanism of heavy metal removal from the aquatic medium using the novel hybrid functionalized cordierite-based ceramic.",
publisher = "Desalination Publ, Hopkinton",
journal = "Desalination and Water Treatment",
title = "Porous cordierite-supported polyethyleneimine composites for nickel(II) and cadmium(II) ions removal",
pages = "296-283",
volume = "192",
doi = "10.5004/dwt.2020.25736"
}
Obradović, N., Rusmirović, J. D., Filipović, S., Kosanović, D., Marinković, A., Radić, D.,& Pavlović, V.. (2020). Porous cordierite-supported polyethyleneimine composites for nickel(II) and cadmium(II) ions removal. in Desalination and Water Treatment
Desalination Publ, Hopkinton., 192, 283-296.
https://doi.org/10.5004/dwt.2020.25736
Obradović N, Rusmirović JD, Filipović S, Kosanović D, Marinković A, Radić D, Pavlović V. Porous cordierite-supported polyethyleneimine composites for nickel(II) and cadmium(II) ions removal. in Desalination and Water Treatment. 2020;192:283-296.
doi:10.5004/dwt.2020.25736 .
Obradović, Nina, Rusmirović, Jelena D., Filipović, Suzana, Kosanović, Darko, Marinković, Aleksandar, Radić, Danka, Pavlović, Vladimir, "Porous cordierite-supported polyethyleneimine composites for nickel(II) and cadmium(II) ions removal" in Desalination and Water Treatment, 192 (2020):283-296,
https://doi.org/10.5004/dwt.2020.25736 . .
1
1
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Raman Spectroscopy as a Tool to Study Genotype-Species Dependence of Tomato-Trichoderma Interaction Effects on Seed Germination

Vukelić, I.; Radić, Danka; Pećinar, Ilinka; Lević, Steva; Racić, Gordana; Panković, D.

(Amer Phytopathological Soc, St Paul, 2019)

TY  - CONF
AU  - Vukelić, I.
AU  - Radić, Danka
AU  - Pećinar, Ilinka
AU  - Lević, Steva
AU  - Racić, Gordana
AU  - Panković, D.
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4920
PB  - Amer Phytopathological Soc, St Paul
C3  - Molecular Plant-Microbe Interactions
T1  - Raman Spectroscopy as a Tool to Study Genotype-Species Dependence of Tomato-Trichoderma Interaction Effects on Seed Germination
EP  - 189
IS  - 10
SP  - 189
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4920
ER  - 
@conference{
author = "Vukelić, I. and Radić, Danka and Pećinar, Ilinka and Lević, Steva and Racić, Gordana and Panković, D.",
year = "2019",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Molecular Plant-Microbe Interactions",
title = "Raman Spectroscopy as a Tool to Study Genotype-Species Dependence of Tomato-Trichoderma Interaction Effects on Seed Germination",
pages = "189-189",
number = "10",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4920"
}
Vukelić, I., Radić, D., Pećinar, I., Lević, S., Racić, G.,& Panković, D.. (2019). Raman Spectroscopy as a Tool to Study Genotype-Species Dependence of Tomato-Trichoderma Interaction Effects on Seed Germination. in Molecular Plant-Microbe Interactions
Amer Phytopathological Soc, St Paul., 32(10), 189-189.
https://hdl.handle.net/21.15107/rcub_agrospace_4920
Vukelić I, Radić D, Pećinar I, Lević S, Racić G, Panković D. Raman Spectroscopy as a Tool to Study Genotype-Species Dependence of Tomato-Trichoderma Interaction Effects on Seed Germination. in Molecular Plant-Microbe Interactions. 2019;32(10):189-189.
https://hdl.handle.net/21.15107/rcub_agrospace_4920 .
Vukelić, I., Radić, Danka, Pećinar, Ilinka, Lević, Steva, Racić, Gordana, Panković, D., "Raman Spectroscopy as a Tool to Study Genotype-Species Dependence of Tomato-Trichoderma Interaction Effects on Seed Germination" in Molecular Plant-Microbe Interactions, 32, no. 10 (2019):189-189,
https://hdl.handle.net/21.15107/rcub_agrospace_4920 .

Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia

Karličić, Vera; Radić, Danka; Jovičić-Petrović, Jelena; Lalević, Blažo; Morina, Filis; Golubović-Ćurguz, Vesna; Raičević, Vera

(Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza, 2017)

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Morina, Filis
AU  - Golubović-Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4466
AB  - Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus x acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara's overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.
PB  - Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza
T2  - Iforest-Biogeosciences and Forestry
T1  - Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia
EP  - 699
SP  - 692
VL  - 10
DO  - 10.3832/ifor2135-010
ER  - 
@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Lalević, Blažo and Morina, Filis and Golubović-Ćurguz, Vesna and Raičević, Vera",
year = "2017",
abstract = "Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus x acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara's overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.",
publisher = "Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza",
journal = "Iforest-Biogeosciences and Forestry",
title = "Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia",
pages = "699-692",
volume = "10",
doi = "10.3832/ifor2135-010"
}
Karličić, V., Radić, D., Jovičić-Petrović, J., Lalević, B., Morina, F., Golubović-Ćurguz, V.,& Raičević, V.. (2017). Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia. in Iforest-Biogeosciences and Forestry
Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza., 10, 692-699.
https://doi.org/10.3832/ifor2135-010
Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Morina F, Golubović-Ćurguz V, Raičević V. Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia. in Iforest-Biogeosciences and Forestry. 2017;10:692-699.
doi:10.3832/ifor2135-010 .
Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Lalević, Blažo, Morina, Filis, Golubović-Ćurguz, Vesna, Raičević, Vera, "Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia" in Iforest-Biogeosciences and Forestry, 10 (2017):692-699,
https://doi.org/10.3832/ifor2135-010 . .
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4

Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism

Radić, Danka; Pavlović, Vera P.; Lazović, Milana; Jovičić-Petrović, Jelena; Karličić, Vera; Lalević, Blažo; Raičević, Vera

(Springer Heidelberg, Heidelberg, 2017)

TY  - JOUR
AU  - Radić, Danka
AU  - Pavlović, Vera P.
AU  - Lazović, Milana
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4484
AB  - Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism
EP  - 21893
IS  - 27
SP  - 21885
VL  - 24
DO  - 10.1007/s11356-017-9817-4
ER  - 
@article{
author = "Radić, Danka and Pavlović, Vera P. and Lazović, Milana and Jovičić-Petrović, Jelena and Karličić, Vera and Lalević, Blažo and Raičević, Vera",
year = "2017",
abstract = "Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism",
pages = "21893-21885",
number = "27",
volume = "24",
doi = "10.1007/s11356-017-9817-4"
}
Radić, D., Pavlović, V. P., Lazović, M., Jovičić-Petrović, J., Karličić, V., Lalević, B.,& Raičević, V.. (2017). Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(27), 21885-21893.
https://doi.org/10.1007/s11356-017-9817-4
Radić D, Pavlović VP, Lazović M, Jovičić-Petrović J, Karličić V, Lalević B, Raičević V. Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research. 2017;24(27):21885-21893.
doi:10.1007/s11356-017-9817-4 .
Radić, Danka, Pavlović, Vera P., Lazović, Milana, Jovičić-Petrović, Jelena, Karličić, Vera, Lalević, Blažo, Raičević, Vera, "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism" in Environmental Science and Pollution Research, 24, no. 27 (2017):21885-21893,
https://doi.org/10.1007/s11356-017-9817-4 . .
1
12
7
11

Formation of Porous Wollastonite-based Ceramics after Sintering With Yeast as the Pore-forming Agent

Obradović, Nina; Filipović, Suzana; Rusmirović, Jelena D.; Postole, Georgeta; Marinković, Aleksandar; Radić, Danka; Rakić, Vesna; Pavlović, Vladimir; Auroux, Aline

(Međunarodni Institut za nauku o sinterovanju, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Nina
AU  - Filipović, Suzana
AU  - Rusmirović, Jelena D.
AU  - Postole, Georgeta
AU  - Marinković, Aleksandar
AU  - Radić, Danka
AU  - Rakić, Vesna
AU  - Pavlović, Vladimir
AU  - Auroux, Aline
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4477
AB  - In this paper, synthesis of porous wollastonite-based ceramics was reported. Ceramic precursor, methylhydrocyclosiloxane, together with micro-sized CaCO3, was used as starting material. After 20 min of ultrasound treatment, and calcination at 250 degrees C for 30 min, yeast as a pore-forming agent was added to the as-obtained powders. Sintering regime was set up based on the results obtained by differential thermal analysis. Prepared mixture was pressed into pallets and sintered at 900 degrees C for 1 h. After the sintering regime, porous wollastonite-based ceramics was obtained. The phase composition of the sintered samples as well as microstructures was analyzed by X-ray diffraction method and SEM. In a batch test, the influence of pH, contact time and initial ion concentration on adsorption efficiency of As+5, Cr+6, and phosphate ions on synthesized wollastonite-based ceramics were studied. Time-dependent adsorption was best described by pseudo-second-order kinetic model and Weber-Morris model that predicted intra-particle diffusion as a rate-controlling step of overall process. High adsorption capacities 39.97, 21.87, and 15.29 mgg(-1) were obtained for As+5, Cr+6, and phosphate ions, respectively.
PB  - Međunarodni Institut za nauku o sinterovanju, Beograd
T2  - Science of Sintering
T1  - Formation of Porous Wollastonite-based Ceramics after Sintering With Yeast as the Pore-forming Agent
EP  - 246
IS  - 3
SP  - 235
VL  - 49
DO  - 10.2298/SOS1703235O
ER  - 
@article{
author = "Obradović, Nina and Filipović, Suzana and Rusmirović, Jelena D. and Postole, Georgeta and Marinković, Aleksandar and Radić, Danka and Rakić, Vesna and Pavlović, Vladimir and Auroux, Aline",
year = "2017",
abstract = "In this paper, synthesis of porous wollastonite-based ceramics was reported. Ceramic precursor, methylhydrocyclosiloxane, together with micro-sized CaCO3, was used as starting material. After 20 min of ultrasound treatment, and calcination at 250 degrees C for 30 min, yeast as a pore-forming agent was added to the as-obtained powders. Sintering regime was set up based on the results obtained by differential thermal analysis. Prepared mixture was pressed into pallets and sintered at 900 degrees C for 1 h. After the sintering regime, porous wollastonite-based ceramics was obtained. The phase composition of the sintered samples as well as microstructures was analyzed by X-ray diffraction method and SEM. In a batch test, the influence of pH, contact time and initial ion concentration on adsorption efficiency of As+5, Cr+6, and phosphate ions on synthesized wollastonite-based ceramics were studied. Time-dependent adsorption was best described by pseudo-second-order kinetic model and Weber-Morris model that predicted intra-particle diffusion as a rate-controlling step of overall process. High adsorption capacities 39.97, 21.87, and 15.29 mgg(-1) were obtained for As+5, Cr+6, and phosphate ions, respectively.",
publisher = "Međunarodni Institut za nauku o sinterovanju, Beograd",
journal = "Science of Sintering",
title = "Formation of Porous Wollastonite-based Ceramics after Sintering With Yeast as the Pore-forming Agent",
pages = "246-235",
number = "3",
volume = "49",
doi = "10.2298/SOS1703235O"
}
Obradović, N., Filipović, S., Rusmirović, J. D., Postole, G., Marinković, A., Radić, D., Rakić, V., Pavlović, V.,& Auroux, A.. (2017). Formation of Porous Wollastonite-based Ceramics after Sintering With Yeast as the Pore-forming Agent. in Science of Sintering
Međunarodni Institut za nauku o sinterovanju, Beograd., 49(3), 235-246.
https://doi.org/10.2298/SOS1703235O
Obradović N, Filipović S, Rusmirović JD, Postole G, Marinković A, Radić D, Rakić V, Pavlović V, Auroux A. Formation of Porous Wollastonite-based Ceramics after Sintering With Yeast as the Pore-forming Agent. in Science of Sintering. 2017;49(3):235-246.
doi:10.2298/SOS1703235O .
Obradović, Nina, Filipović, Suzana, Rusmirović, Jelena D., Postole, Georgeta, Marinković, Aleksandar, Radić, Danka, Rakić, Vesna, Pavlović, Vladimir, Auroux, Aline, "Formation of Porous Wollastonite-based Ceramics after Sintering With Yeast as the Pore-forming Agent" in Science of Sintering, 49, no. 3 (2017):235-246,
https://doi.org/10.2298/SOS1703235O . .
4
2
6

Preparation of cordierite-based adsorbents for water purification

Obradović, Nina; Kosanović, Darko; Filipović, Suzana; Rusmirović, Jelena; Marinković, Aleksandar D.; Radić, Danka; Pavlović, Vladimir B.

(Belgrade : Serbian Ceramic Society, 2017)

TY  - CONF
AU  - Obradović, Nina
AU  - Kosanović, Darko
AU  - Filipović, Suzana
AU  - Rusmirović, Jelena
AU  - Marinković, Aleksandar D.
AU  - Radić, Danka
AU  - Pavlović, Vladimir B.
PY  - 2017
UR  - http://dais.sanu.ac.rs/123456789/4776
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5737
AB  - In this study, porous cordierite-based ceramics was synthesized. In the first step, MgO, Al2O3, and SiO2 (all Aldrich, p.a.) were used. MgO and Al2O3 powders were calcined at 1000 oC for 2 h, in order to avoid hydroxides. They were mixed in 2:2:5 molar ratio, in order to form cordierite, Mg2Al4Si5O18. Ethanol was added to the mixture, and then milled in a Fritsch Pulverisette planetary mill, with 300 rpm, in air atmosfere. Times of activation were 0, 10, 40, and 80 min, while balls and vessels were made from ZrO2, and powder to balls mass ratio was 40:1. After milling, powders were dried, and then pressed under 3 t/cm2. Pallets with 8 mm radius were sintered in air at 1350 oC, for 2 h, heating rate was 20 oC/min. Sintered samples were crashed and sieved. In the secons step, the as-prepared cordierite was mixed with 20 wt.% yeast (0.1 g yeast + 0.4 g cordierite per sample). The other mixture was with 20 wt.% nanocellulose (0.1 g NC + 0.4 g cordierite per sample). Both mixtures were pressed into pallets under 5 t/cm2 and sintered at 700 oC, with 5 oC/min heating rate, in air atmosfere. During the second sintering regime, porous cordierite-based ceramics was obtained. The phase composition of the sintered samples as well as microstructures was analyzed by the means of X-ray diffraction method and SEM. Cordierite was the most abundant phase in all sintered samples. It was observed that addition of different pore-forming agent resulted in significantly different microstructures.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017
T1  - Preparation of cordierite-based adsorbents for water purification
EP  - 71
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_dais_4776
ER  - 
@conference{
author = "Obradović, Nina and Kosanović, Darko and Filipović, Suzana and Rusmirović, Jelena and Marinković, Aleksandar D. and Radić, Danka and Pavlović, Vladimir B.",
year = "2017",
abstract = "In this study, porous cordierite-based ceramics was synthesized. In the first step, MgO, Al2O3, and SiO2 (all Aldrich, p.a.) were used. MgO and Al2O3 powders were calcined at 1000 oC for 2 h, in order to avoid hydroxides. They were mixed in 2:2:5 molar ratio, in order to form cordierite, Mg2Al4Si5O18. Ethanol was added to the mixture, and then milled in a Fritsch Pulverisette planetary mill, with 300 rpm, in air atmosfere. Times of activation were 0, 10, 40, and 80 min, while balls and vessels were made from ZrO2, and powder to balls mass ratio was 40:1. After milling, powders were dried, and then pressed under 3 t/cm2. Pallets with 8 mm radius were sintered in air at 1350 oC, for 2 h, heating rate was 20 oC/min. Sintered samples were crashed and sieved. In the secons step, the as-prepared cordierite was mixed with 20 wt.% yeast (0.1 g yeast + 0.4 g cordierite per sample). The other mixture was with 20 wt.% nanocellulose (0.1 g NC + 0.4 g cordierite per sample). Both mixtures were pressed into pallets under 5 t/cm2 and sintered at 700 oC, with 5 oC/min heating rate, in air atmosfere. During the second sintering regime, porous cordierite-based ceramics was obtained. The phase composition of the sintered samples as well as microstructures was analyzed by the means of X-ray diffraction method and SEM. Cordierite was the most abundant phase in all sintered samples. It was observed that addition of different pore-forming agent resulted in significantly different microstructures.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017",
title = "Preparation of cordierite-based adsorbents for water purification",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_dais_4776"
}
Obradović, N., Kosanović, D., Filipović, S., Rusmirović, J., Marinković, A. D., Radić, D.,& Pavlović, V. B.. (2017). Preparation of cordierite-based adsorbents for water purification. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017
Belgrade : Serbian Ceramic Society., 71-71.
https://hdl.handle.net/21.15107/rcub_dais_4776
Obradović N, Kosanović D, Filipović S, Rusmirović J, Marinković AD, Radić D, Pavlović VB. Preparation of cordierite-based adsorbents for water purification. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017. 2017;:71-71.
https://hdl.handle.net/21.15107/rcub_dais_4776 .
Obradović, Nina, Kosanović, Darko, Filipović, Suzana, Rusmirović, Jelena, Marinković, Aleksandar D., Radić, Danka, Pavlović, Vladimir B., "Preparation of cordierite-based adsorbents for water purification" in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017 (2017):71-71,
https://hdl.handle.net/21.15107/rcub_dais_4776 .

Isolation and characterization of bacteria and yeasts from contaminated soil

Karličić, Vera; Radić, Danka; Jovičić-Petrović, Jelena; Lalević, Blažo; Jovanović, Ljubinko; Kiković, Dragan; Raičević, Vera

(Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd, 2016)

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Jovanović, Ljubinko
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4247
AB  - Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeast isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indoleacetic acid, siderophores and solubilization of inorganic phosphate). As a result, three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeast isolates (Candida utilis and Candida tropicalis) were recognized as PGP strains. Among them, Serratia sp. showed the highest indole production (25.5 μg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a result of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements.
AB  - Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Isolation and characterization of bacteria and yeasts from contaminated soil
T1  - Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta
EP  - 256
IS  - 3
SP  - 247
VL  - 61
DO  - 10.2298/JAS1603247K
ER  - 
@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Lalević, Blažo and Jovanović, Ljubinko and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeast isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indoleacetic acid, siderophores and solubilization of inorganic phosphate). As a result, three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeast isolates (Candida utilis and Candida tropicalis) were recognized as PGP strains. Among them, Serratia sp. showed the highest indole production (25.5 μg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a result of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements., Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Isolation and characterization of bacteria and yeasts from contaminated soil, Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta",
pages = "256-247",
number = "3",
volume = "61",
doi = "10.2298/JAS1603247K"
}
Karličić, V., Radić, D., Jovičić-Petrović, J., Lalević, B., Jovanović, L., Kiković, D.,& Raičević, V.. (2016). Isolation and characterization of bacteria and yeasts from contaminated soil. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 61(3), 247-256.
https://doi.org/10.2298/JAS1603247K
Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Jovanović L, Kiković D, Raičević V. Isolation and characterization of bacteria and yeasts from contaminated soil. in Journal of Agricultural Sciences (Belgrade). 2016;61(3):247-256.
doi:10.2298/JAS1603247K .
Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Lalević, Blažo, Jovanović, Ljubinko, Kiković, Dragan, Raičević, Vera, "Isolation and characterization of bacteria and yeasts from contaminated soil" in Journal of Agricultural Sciences (Belgrade), 61, no. 3 (2016):247-256,
https://doi.org/10.2298/JAS1603247K . .
5

Raman spectroscopy and determination of soil yeasts

Pavlović, Vera P.; Radić, Danka; Karličić, Vera; Lalević, Blažo; Lević, Steva; Raičević, Vera

(Inženjersko društvo za koroziju, Beograd, 2016)

TY  - JOUR
AU  - Pavlović, Vera P.
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Lalević, Blažo
AU  - Lević, Steva
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4232
AB  - In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions.
AB  - U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Raman spectroscopy and determination of soil yeasts
T1  - Raman spektroskopija i determinacija zemljišnih kvasaca
EP  - 459
IS  - 3
SP  - 455
VL  - 57
DO  - 10.5937/ZasMat1603455P
ER  - 
@article{
author = "Pavlović, Vera P. and Radić, Danka and Karličić, Vera and Lalević, Blažo and Lević, Steva and Raičević, Vera",
year = "2016",
abstract = "In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions., U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Raman spectroscopy and determination of soil yeasts, Raman spektroskopija i determinacija zemljišnih kvasaca",
pages = "459-455",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603455P"
}
Pavlović, V. P., Radić, D., Karličić, V., Lalević, B., Lević, S.,& Raičević, V.. (2016). Raman spectroscopy and determination of soil yeasts. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 455-459.
https://doi.org/10.5937/ZasMat1603455P
Pavlović VP, Radić D, Karličić V, Lalević B, Lević S, Raičević V. Raman spectroscopy and determination of soil yeasts. in Zaštita materijala. 2016;57(3):455-459.
doi:10.5937/ZasMat1603455P .
Pavlović, Vera P., Radić, Danka, Karličić, Vera, Lalević, Blažo, Lević, Steva, Raičević, Vera, "Raman spectroscopy and determination of soil yeasts" in Zaštita materijala, 57, no. 3 (2016):455-459,
https://doi.org/10.5937/ZasMat1603455P . .

Microbial diversity of soil contaminated with high content of heavy metals

Ilić, Dora; Radić, Danka; Karličić, Vera; Jovičić-Petrović, Jelena; Kiković, Dragan; Lalević, Blažo; Raičević, Vera

(Inženjersko društvo za koroziju, Beograd, 2016)

TY  - JOUR
AU  - Ilić, Dora
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Kiković, Dragan
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4233
AB  - Except of benefits for human population, several industrial activities have a negative consequences i.e. increasing of heavy metal content and changes in diversity of living organisms in environment. The aim of this work was to examinated the influence of high content of heavy metals on microbial diversity of soil. Researches was conducted at locations of former factory 'Rog' (Ljubljana, Republic of Slovenia), by taking of four samples of contaminated soil, while the control was uncontaminated soil sample. Determination of microbial diversity (abundance of bacteria, fungi and actinomycetes) was performed using the standard methodology. The results of research showed that abundance of microorganisms depends on location and presence/absence of contamination. In control treatment abundance of all examinated groups of microorganisms was higher compared with other samples. The bacterial number in contaminated samples was from 1.21 to 6.88x106 CFU/g, the number of fungi was from 1,08 to 30,30x103 CFU/g, while actinomycetes number from 302,15 to 381,82x103 CFU/g. In control treatment, bacterial number was 17,45x106 CFU/g, fungi number 54,02x103 CFU/g and actinomycetes number 481,91x103 CFU/g. These results confirms the importance of microbial diversity research on contaminated locations and potential applications of bioremediation technologies on locations of former factory 'Rog'.
AB  - Mnogobrojne industrijske aktivnosti, osim koristi za ljudsku populaciju, imale su za posledicu povećanje sadržaja teških metala i promene diverziteta živih organizama u životnoj sredini. Cilj ovog rada bio je ispitivanje uticaja visokih koncentracija teških metala na mikrobni diverzitet zemljišta. Istraživanja su obavljena na području bivše fabrike 'Rog' (Ljubljana, Republika Slovenija), gde su uzeta četiri uzorka kontaminiranog zemljišta, dok je kontrolni uzorak predstavljao nekontaminirano zemljište. Ispitivanje mikrobnog diverziteta (brojnost bakterija, gljiva i aktinomiceta) obavljeno je primenom standardnih metoda. Rezultati istraživanja ukazuju da je brojnost mikroorganizama zavisila od lokacije i prusustva/odsustva kontaminacije. U kontrolnoj varijanti brojnost svih ispitivanih grupa mikroorganizama bila je veća u odnosu na ostale uzorke. Brojnost bakterija u kontaminiranim uzorcima iznosila je od 1,21-6,88x106 CFU/g, gljiva 1,08-30,30x103 CFU/g i aktinomiceta 302,15-381,82x103 CFU/g. U kontrolnoj varijanti broj bakterija iznosio je 17,45x106 CFU/g, gljiva 54,02x103 CFU/g i aktinomiceta 481,91x103 CFU/g. Ovi rezultati ukuzuju na značaj ispitivanja mikrobnog diverziteta na kontaminiranim lokacijama i predstavljaju osnovu za potencijalne aplikacije bioremedijacionih tehnologija na području bivše fabrike 'Rog'.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Microbial diversity of soil contaminated with high content of heavy metals
T1  - Mikrobni diverzitet zemljišta kontaminiranog visokim sadržajem teških metala
EP  - 387
IS  - 3
SP  - 383
VL  - 57
DO  - 10.5937/ZasMat1603383I
ER  - 
@article{
author = "Ilić, Dora and Radić, Danka and Karličić, Vera and Jovičić-Petrović, Jelena and Kiković, Dragan and Lalević, Blažo and Raičević, Vera",
year = "2016",
abstract = "Except of benefits for human population, several industrial activities have a negative consequences i.e. increasing of heavy metal content and changes in diversity of living organisms in environment. The aim of this work was to examinated the influence of high content of heavy metals on microbial diversity of soil. Researches was conducted at locations of former factory 'Rog' (Ljubljana, Republic of Slovenia), by taking of four samples of contaminated soil, while the control was uncontaminated soil sample. Determination of microbial diversity (abundance of bacteria, fungi and actinomycetes) was performed using the standard methodology. The results of research showed that abundance of microorganisms depends on location and presence/absence of contamination. In control treatment abundance of all examinated groups of microorganisms was higher compared with other samples. The bacterial number in contaminated samples was from 1.21 to 6.88x106 CFU/g, the number of fungi was from 1,08 to 30,30x103 CFU/g, while actinomycetes number from 302,15 to 381,82x103 CFU/g. In control treatment, bacterial number was 17,45x106 CFU/g, fungi number 54,02x103 CFU/g and actinomycetes number 481,91x103 CFU/g. These results confirms the importance of microbial diversity research on contaminated locations and potential applications of bioremediation technologies on locations of former factory 'Rog'., Mnogobrojne industrijske aktivnosti, osim koristi za ljudsku populaciju, imale su za posledicu povećanje sadržaja teških metala i promene diverziteta živih organizama u životnoj sredini. Cilj ovog rada bio je ispitivanje uticaja visokih koncentracija teških metala na mikrobni diverzitet zemljišta. Istraživanja su obavljena na području bivše fabrike 'Rog' (Ljubljana, Republika Slovenija), gde su uzeta četiri uzorka kontaminiranog zemljišta, dok je kontrolni uzorak predstavljao nekontaminirano zemljište. Ispitivanje mikrobnog diverziteta (brojnost bakterija, gljiva i aktinomiceta) obavljeno je primenom standardnih metoda. Rezultati istraživanja ukazuju da je brojnost mikroorganizama zavisila od lokacije i prusustva/odsustva kontaminacije. U kontrolnoj varijanti brojnost svih ispitivanih grupa mikroorganizama bila je veća u odnosu na ostale uzorke. Brojnost bakterija u kontaminiranim uzorcima iznosila je od 1,21-6,88x106 CFU/g, gljiva 1,08-30,30x103 CFU/g i aktinomiceta 302,15-381,82x103 CFU/g. U kontrolnoj varijanti broj bakterija iznosio je 17,45x106 CFU/g, gljiva 54,02x103 CFU/g i aktinomiceta 481,91x103 CFU/g. Ovi rezultati ukuzuju na značaj ispitivanja mikrobnog diverziteta na kontaminiranim lokacijama i predstavljaju osnovu za potencijalne aplikacije bioremedijacionih tehnologija na području bivše fabrike 'Rog'.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Microbial diversity of soil contaminated with high content of heavy metals, Mikrobni diverzitet zemljišta kontaminiranog visokim sadržajem teških metala",
pages = "387-383",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603383I"
}
Ilić, D., Radić, D., Karličić, V., Jovičić-Petrović, J., Kiković, D., Lalević, B.,& Raičević, V.. (2016). Microbial diversity of soil contaminated with high content of heavy metals. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 383-387.
https://doi.org/10.5937/ZasMat1603383I
Ilić D, Radić D, Karličić V, Jovičić-Petrović J, Kiković D, Lalević B, Raičević V. Microbial diversity of soil contaminated with high content of heavy metals. in Zaštita materijala. 2016;57(3):383-387.
doi:10.5937/ZasMat1603383I .
Ilić, Dora, Radić, Danka, Karličić, Vera, Jovičić-Petrović, Jelena, Kiković, Dragan, Lalević, Blažo, Raičević, Vera, "Microbial diversity of soil contaminated with high content of heavy metals" in Zaštita materijala, 57, no. 3 (2016):383-387,
https://doi.org/10.5937/ZasMat1603383I . .

Macrophytes as remediation technology in improving ludas lake sediment

Radić, Danka; Gujaničić, Vera; Petricević, Jelena; Raičević, Vera; Lalević, Blažo; Rudić, Željka; Božić, Mile

(Parlar Scientific Publications (P S P), Freising, 2013)

TY  - JOUR
AU  - Radić, Danka
AU  - Gujaničić, Vera
AU  - Petricević, Jelena
AU  - Raičević, Vera
AU  - Lalević, Blažo
AU  - Rudić, Željka
AU  - Božić, Mile
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3158
AB  - Ludas lake is Pannonian plain Lake settled 4 km from Palic. The lake is characterized by thick sediment layers with high content of phosphorus and nitrogen. These thick layers are the result of accelerated eutrophication and cause lost of biodiversity and disruption of ecosystem. Numerous methods can be used to overcome the problem of accelerated eutrophication (sediment removal, phosphorus inactivation etc.), but those methods have a lot deficiencies. To overcome that, the use of macrophytes to resolve the problem of eutophication becomes more and more popular. The main aim of this paper was to examine the germination possibilities of mustard (Sinapis alba L.), alfalfa (Medicago sativa L.), oat (Avena sativa L.) and lettuce (Lactuca sativa L.) on Ludas's sediment as a substrate. This was used as a pre-screening test for plants suitability for further phytoremediation. The results showed that plant species can germinate and grow in early stages in such a substrate, so they can be recommended for sediment phytoremediation.
PB  - Parlar Scientific Publications (P S P), Freising
T2  - Fresenius Environmental Bulletin
T1  - Macrophytes as remediation technology in improving ludas lake sediment
EP  - 1791
IS  - 6
SP  - 1787
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3158
ER  - 
@article{
author = "Radić, Danka and Gujaničić, Vera and Petricević, Jelena and Raičević, Vera and Lalević, Blažo and Rudić, Željka and Božić, Mile",
year = "2013",
abstract = "Ludas lake is Pannonian plain Lake settled 4 km from Palic. The lake is characterized by thick sediment layers with high content of phosphorus and nitrogen. These thick layers are the result of accelerated eutrophication and cause lost of biodiversity and disruption of ecosystem. Numerous methods can be used to overcome the problem of accelerated eutrophication (sediment removal, phosphorus inactivation etc.), but those methods have a lot deficiencies. To overcome that, the use of macrophytes to resolve the problem of eutophication becomes more and more popular. The main aim of this paper was to examine the germination possibilities of mustard (Sinapis alba L.), alfalfa (Medicago sativa L.), oat (Avena sativa L.) and lettuce (Lactuca sativa L.) on Ludas's sediment as a substrate. This was used as a pre-screening test for plants suitability for further phytoremediation. The results showed that plant species can germinate and grow in early stages in such a substrate, so they can be recommended for sediment phytoremediation.",
publisher = "Parlar Scientific Publications (P S P), Freising",
journal = "Fresenius Environmental Bulletin",
title = "Macrophytes as remediation technology in improving ludas lake sediment",
pages = "1791-1787",
number = "6",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3158"
}
Radić, D., Gujaničić, V., Petricević, J., Raičević, V., Lalević, B., Rudić, Ž.,& Božić, M.. (2013). Macrophytes as remediation technology in improving ludas lake sediment. in Fresenius Environmental Bulletin
Parlar Scientific Publications (P S P), Freising., 22(6), 1787-1791.
https://hdl.handle.net/21.15107/rcub_agrospace_3158
Radić D, Gujaničić V, Petricević J, Raičević V, Lalević B, Rudić Ž, Božić M. Macrophytes as remediation technology in improving ludas lake sediment. in Fresenius Environmental Bulletin. 2013;22(6):1787-1791.
https://hdl.handle.net/21.15107/rcub_agrospace_3158 .
Radić, Danka, Gujaničić, Vera, Petricević, Jelena, Raičević, Vera, Lalević, Blažo, Rudić, Željka, Božić, Mile, "Macrophytes as remediation technology in improving ludas lake sediment" in Fresenius Environmental Bulletin, 22, no. 6 (2013):1787-1791,
https://hdl.handle.net/21.15107/rcub_agrospace_3158 .
5

The possibility of using macrophytes in lake Palić sediment remediation

Petričević, J.; Gujaničić, Vera; Radić, Danka; Lalević, Blažo; Božić, M.; Rudić, Željka; Raičević, Vera

(Srpsko biološko društvo, Beograd, i dr., 2012)

TY  - JOUR
AU  - Petričević, J.
AU  - Gujaničić, Vera
AU  - Radić, Danka
AU  - Lalević, Blažo
AU  - Božić, M.
AU  - Rudić, Željka
AU  - Raičević, Vera
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3068
AB  - Lake Palić is a typical, shallow Pannonian plain lake, with thick sediment layers and high content of phosphorus and nitrogen. The thick layers are the result of accelerated eutrophication, and cause biodiversity loss and disruption of the ecosystem. Numerous methods can be used to overcome the problem of accelerated eutrophication, (sediment removal, phosphorus inactivation etc.). However, these methods have many deficiencies. Lately, using macrophytes as a way to resolve this problem is becoming more and more popular. The aim of this work was to examine the germination possibilities of white mustard (Sinapis alba L.), alfalfa (Medicago sativa L.), oats (Avena sativa L.) and lettuce (Lactuca sativa L.) on Lake Palić’s sediment, as a prescreening test for their suitability for further phytoremediation. The results show that plant species can germinate and grow in early stages in such substrates, so they can be recommended for sediment phytoremediation.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The possibility of using macrophytes in lake Palić sediment remediation
EP  - 1486
IS  - 4
SP  - 1481
VL  - 64
DO  - 10.2298/ABS1204481P
ER  - 
@article{
author = "Petričević, J. and Gujaničić, Vera and Radić, Danka and Lalević, Blažo and Božić, M. and Rudić, Željka and Raičević, Vera",
year = "2012",
abstract = "Lake Palić is a typical, shallow Pannonian plain lake, with thick sediment layers and high content of phosphorus and nitrogen. The thick layers are the result of accelerated eutrophication, and cause biodiversity loss and disruption of the ecosystem. Numerous methods can be used to overcome the problem of accelerated eutrophication, (sediment removal, phosphorus inactivation etc.). However, these methods have many deficiencies. Lately, using macrophytes as a way to resolve this problem is becoming more and more popular. The aim of this work was to examine the germination possibilities of white mustard (Sinapis alba L.), alfalfa (Medicago sativa L.), oats (Avena sativa L.) and lettuce (Lactuca sativa L.) on Lake Palić’s sediment, as a prescreening test for their suitability for further phytoremediation. The results show that plant species can germinate and grow in early stages in such substrates, so they can be recommended for sediment phytoremediation.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The possibility of using macrophytes in lake Palić sediment remediation",
pages = "1486-1481",
number = "4",
volume = "64",
doi = "10.2298/ABS1204481P"
}
Petričević, J., Gujaničić, V., Radić, D., Lalević, B., Božić, M., Rudić, Ž.,& Raičević, V.. (2012). The possibility of using macrophytes in lake Palić sediment remediation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1481-1486.
https://doi.org/10.2298/ABS1204481P
Petričević J, Gujaničić V, Radić D, Lalević B, Božić M, Rudić Ž, Raičević V. The possibility of using macrophytes in lake Palić sediment remediation. in Archives of Biological Sciences. 2012;64(4):1481-1486.
doi:10.2298/ABS1204481P .
Petričević, J., Gujaničić, Vera, Radić, Danka, Lalević, Blažo, Božić, M., Rudić, Željka, Raičević, Vera, "The possibility of using macrophytes in lake Palić sediment remediation" in Archives of Biological Sciences, 64, no. 4 (2012):1481-1486,
https://doi.org/10.2298/ABS1204481P . .
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