TY  - JOUR
AU  - Vunduk, Jovana
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Mohamad, Shaiful Azuar
AU  - Abd Halim, Nur Hafizati
AU  - Dzomir, Ahmad Zainuri Mohd
AU  - Zizak, Zeljko
AU  - Klaus, Anita
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4940
AB  - Foodborne bacteria biofilms present a major concern for the food industry. Although their numerous biological activities are well established, there is little research to date on the use of polysaccharides of mushroom origin as a possible solution for preventing biofilm formation. Therefore, the aim of this study was to examine the anti adhesion and antibiofilm effects of several types of Pleurotus flabellatus strain Mynuk polysaccharide extracts (PFSMpe), produced by air-lift submerged fermentation, against American Type Culture Collection (ATCC) and clinical strains of foodborne bacteria, as well as the cytotoxicity of these extracts. PFSMpe exhibited antiadhesion activity toward clinical isolates, and the percentage of adhesion inhibition was highest for water (WE) and exopolysaccharide (EXOPE) extracts (> 50%) against Enterococcus faecalis. Selected ATCC strains were more resistant than clinical strains, indicating the antiquorum sensing mechanism of PFSMpe action. Antibiofilm activity was similar to antiadhesion activity and WE showed the strongest effect, again on E. faecalis. Differences in antiadhesion and antibiofilm effects of PFSMpe may be explained by differences in chemical composition, with crude extracts showing greater efficiency due to a synergistic effect. PFSMpe did not exhibit cyctotoxic activity against normal human cell lines. Overall, the findings of this study show that PFSMpe represents a promising novel strategy against bacterial biofilms.
PB  - Elsevier, Amsterdam
T2  - LWT-Food Science and Technology
T1  - Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens
VL  - 112
DO  - 10.1016/j.lwt.2019.05.119
ER  - 

@article{
author = "Vunduk, Jovana and Wan-Mohtar, Wan Abd Al Qadr Imad and Mohamad, Shaiful Azuar and Abd Halim, Nur Hafizati and Dzomir, Ahmad Zainuri Mohd and Zizak, Zeljko and Klaus, Anita",
year = "2019",
abstract = "Foodborne bacteria biofilms present a major concern for the food industry. Although their numerous biological activities are well established, there is little research to date on the use of polysaccharides of mushroom origin as a possible solution for preventing biofilm formation. Therefore, the aim of this study was to examine the anti adhesion and antibiofilm effects of several types of Pleurotus flabellatus strain Mynuk polysaccharide extracts (PFSMpe), produced by air-lift submerged fermentation, against American Type Culture Collection (ATCC) and clinical strains of foodborne bacteria, as well as the cytotoxicity of these extracts. PFSMpe exhibited antiadhesion activity toward clinical isolates, and the percentage of adhesion inhibition was highest for water (WE) and exopolysaccharide (EXOPE) extracts (> 50%) against Enterococcus faecalis. Selected ATCC strains were more resistant than clinical strains, indicating the antiquorum sensing mechanism of PFSMpe action. Antibiofilm activity was similar to antiadhesion activity and WE showed the strongest effect, again on E. faecalis. Differences in antiadhesion and antibiofilm effects of PFSMpe may be explained by differences in chemical composition, with crude extracts showing greater efficiency due to a synergistic effect. PFSMpe did not exhibit cyctotoxic activity against normal human cell lines. Overall, the findings of this study show that PFSMpe represents a promising novel strategy against bacterial biofilms.",
publisher = "Elsevier, Amsterdam",
journal = "LWT-Food Science and Technology",
title = "Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens",
volume = "112",
doi = "10.1016/j.lwt.2019.05.119"
}

Vunduk, J., Wan-Mohtar, W. A. A. Q. I., Mohamad, S. A., Abd Halim, N. H., Dzomir, A. Z. M., Zizak, Z.,& Klaus, A.. (2019). Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens. in LWT-Food Science and Technology
Elsevier, Amsterdam., 112.
https://doi.org/10.1016/j.lwt.2019.05.119

Vunduk J, Wan-Mohtar WAAQI, Mohamad SA, Abd Halim NH, Dzomir AZM, Zizak Z, Klaus A. Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens. in LWT-Food Science and Technology. 2019;112.
doi:10.1016/j.lwt.2019.05.119 .

Vunduk, Jovana, Wan-Mohtar, Wan Abd Al Qadr Imad, Mohamad, Shaiful Azuar, Abd Halim, Nur Hafizati, Dzomir, Ahmad Zainuri Mohd, Zizak, Zeljko, Klaus, Anita, "Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens" in LWT-Food Science and Technology, 112 (2019),
https://doi.org/10.1016/j.lwt.2019.05.119 . .