TY  - JOUR
AU  - Madzovska-Malagurski, I.
AU  - Vukašinović-Sekulić, M.
AU  - Kostić, D.
AU  - Lević, Steva
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4181
AB  - The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.
PB  - IOP Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Towards antimicrobial yet bioactive Cu-alginate hydrogels
IS  - 3
VL  - 11
DO  - 10.1088/1748-6041/11/3/035015
ER  - 

@article{
author = "Madzovska-Malagurski, I. and Vukašinović-Sekulić, M. and Kostić, D. and Lević, Steva",
year = "2016",
abstract = "The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Towards antimicrobial yet bioactive Cu-alginate hydrogels",
number = "3",
volume = "11",
doi = "10.1088/1748-6041/11/3/035015"
}

Madzovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D.,& Lević, S.. (2016). Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials
IOP Publishing Ltd, Bristol., 11(3).
https://doi.org/10.1088/1748-6041/11/3/035015

Madzovska-Malagurski I, Vukašinović-Sekulić M, Kostić D, Lević S. Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials. 2016;11(3).
doi:10.1088/1748-6041/11/3/035015 .

Madzovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D., Lević, Steva, "Towards antimicrobial yet bioactive Cu-alginate hydrogels" in Biomedical Materials, 11, no. 3 (2016),
https://doi.org/10.1088/1748-6041/11/3/035015 . .

TY  - JOUR
AU  - Lujić, J.
AU  - Matavulj, M.
AU  - Poleksić, Vesna
AU  - Rašković, Božidar
AU  - Marinović, Z.
AU  - Kostić, D.
AU  - Miljanović, B.
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3792
AB  - The study evaluated the effects of waterborne pollutants from the Tami River on gill histology and possible differences in gill reaction patterns between three freshwater fish species, pike Esox lucius L. 1758, pike-perch Sander lucioperca (L. 1758) and wels catfish Silurus glanis L. 1758 from the Tami River. Gills from analysed fish species showed moderate to intense histopathological alterations. The most frequent progressive alteration was hyperplasia of epithelium, whereas the most frequent regressive alteration was epithelial lifting. Circulatory disturbances were most often manifested in the form of hyperaemia. During comparative analysis, differences in gill indices, reaction and alteration indices, as well as in gill and filament prevalence between analysed species, were observed. Although all analysed fish species did show both progressive and regressive alterations, there was a significant difference in the level of expression of these reaction patterns. Gill index obtained for pike clearly stands out as the lowest. Wels catfish showed the highest progressive reaction index, significantly higher in comparison with the other two species (P lt 0.05), while pike-perch showed the highest regressive reaction index, also significantly higher in comparison with the other species (P lt 0.001). These results may implicate species-specific gill reactions and thus present a useful tool for better understanding toxic mechanisms of various pollutants.
PB  - Wiley-Blackwell, Hoboken
T2  - Anatomia Histologia Embryologia
T1  - Gill Reaction to Pollutants from the Tamis River in Three Freshwater Fish Species, Esox lucius L. 1758, Sander lucioperca (L. 1758) and Silurus glanis L. 1758: A Comparative Study
EP  - 137
IS  - 2
SP  - 128
VL  - 44
DO  - 10.1111/ahe.12119
ER  - 

@article{
author = "Lujić, J. and Matavulj, M. and Poleksić, Vesna and Rašković, Božidar and Marinović, Z. and Kostić, D. and Miljanović, B.",
year = "2015",
abstract = "The study evaluated the effects of waterborne pollutants from the Tami River on gill histology and possible differences in gill reaction patterns between three freshwater fish species, pike Esox lucius L. 1758, pike-perch Sander lucioperca (L. 1758) and wels catfish Silurus glanis L. 1758 from the Tami River. Gills from analysed fish species showed moderate to intense histopathological alterations. The most frequent progressive alteration was hyperplasia of epithelium, whereas the most frequent regressive alteration was epithelial lifting. Circulatory disturbances were most often manifested in the form of hyperaemia. During comparative analysis, differences in gill indices, reaction and alteration indices, as well as in gill and filament prevalence between analysed species, were observed. Although all analysed fish species did show both progressive and regressive alterations, there was a significant difference in the level of expression of these reaction patterns. Gill index obtained for pike clearly stands out as the lowest. Wels catfish showed the highest progressive reaction index, significantly higher in comparison with the other two species (P lt 0.05), while pike-perch showed the highest regressive reaction index, also significantly higher in comparison with the other species (P lt 0.001). These results may implicate species-specific gill reactions and thus present a useful tool for better understanding toxic mechanisms of various pollutants.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Anatomia Histologia Embryologia",
title = "Gill Reaction to Pollutants from the Tamis River in Three Freshwater Fish Species, Esox lucius L. 1758, Sander lucioperca (L. 1758) and Silurus glanis L. 1758: A Comparative Study",
pages = "137-128",
number = "2",
volume = "44",
doi = "10.1111/ahe.12119"
}

Lujić, J., Matavulj, M., Poleksić, V., Rašković, B., Marinović, Z., Kostić, D.,& Miljanović, B.. (2015). Gill Reaction to Pollutants from the Tamis River in Three Freshwater Fish Species, Esox lucius L. 1758, Sander lucioperca (L. 1758) and Silurus glanis L. 1758: A Comparative Study. in Anatomia Histologia Embryologia
Wiley-Blackwell, Hoboken., 44(2), 128-137.
https://doi.org/10.1111/ahe.12119

Lujić J, Matavulj M, Poleksić V, Rašković B, Marinović Z, Kostić D, Miljanović B. Gill Reaction to Pollutants from the Tamis River in Three Freshwater Fish Species, Esox lucius L. 1758, Sander lucioperca (L. 1758) and Silurus glanis L. 1758: A Comparative Study. in Anatomia Histologia Embryologia. 2015;44(2):128-137.
doi:10.1111/ahe.12119 .

Lujić, J., Matavulj, M., Poleksić, Vesna, Rašković, Božidar, Marinović, Z., Kostić, D., Miljanović, B., "Gill Reaction to Pollutants from the Tamis River in Three Freshwater Fish Species, Esox lucius L. 1758, Sander lucioperca (L. 1758) and Silurus glanis L. 1758: A Comparative Study" in Anatomia Histologia Embryologia, 44, no. 2 (2015):128-137,
https://doi.org/10.1111/ahe.12119 . .