TY  - JOUR
AU  - Žilić, Sladjana
AU  - Janković, Marijana
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
AU  - Konić-Ristić, Aleksandra
AU  - Hadži-Tašković-Šukalović, Vesna
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4090
AB  - The aim was to determine the effect of steeping and sprouting on wheat grain proteins and the functional consequences in this regard. The solubility of proteins and the polypeptide composition of albumins, globulins, gliadins and glutenins were determined, as well as the content of non-protein nitrogen and free sulfhydryl groups (-SH), and the activity of peroxidase (POD) and lipoxygenase (LOX). In addition, the pasting viscosity of flour and protein bioactivity such as antioxidant capacity and immunoreactivity were evaluated. The increase of non-protein nitrogen and free -SH groups by about 62.09 and 96.7%, respectively, as well as the decrease of albumin + globulin polypeptides with a molecular weight over 85.94 kDa and between 85.94-48.00 kDa by about 34 and 8.7%, respectively, were the most notable changes observed in the flour from whole sprouted wheat that clearly point to the intensive protein hydrolysis. The reduction of disulfide bonds and increased concentrations of free -SH groups significantly modify the visco-elastic properties of gliadins and glutenins causing pasting viscosity reduction. However, sprouted wheat flour could be considered as a potential food ingredient because of its improved antioxidant capacity that is a result of protein hydrolysis inter alia. As protein modification induced by steeping may have beneficial effects on the antigenicity of the glutenin fraction, this kind of wheat pretreatment can represent a putative strategy in the dietary modulation of diseases related to glutenin immunoreactivity, e.g. dermatitis herpetiformis.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Effects of enzyme activities during steeping and sprouting on the solubility and composition of proteins, their bioactivity and relationship with the bread making quality of wheat flour
EP  - 4331
IS  - 10
SP  - 4323
VL  - 7
DO  - 10.1039/c6fo01095d
ER  - 

@article{
author = "Žilić, Sladjana and Janković, Marijana and Barać, Miroljub and Pešić, Mirjana and Konić-Ristić, Aleksandra and Hadži-Tašković-Šukalović, Vesna",
year = "2016",
abstract = "The aim was to determine the effect of steeping and sprouting on wheat grain proteins and the functional consequences in this regard. The solubility of proteins and the polypeptide composition of albumins, globulins, gliadins and glutenins were determined, as well as the content of non-protein nitrogen and free sulfhydryl groups (-SH), and the activity of peroxidase (POD) and lipoxygenase (LOX). In addition, the pasting viscosity of flour and protein bioactivity such as antioxidant capacity and immunoreactivity were evaluated. The increase of non-protein nitrogen and free -SH groups by about 62.09 and 96.7%, respectively, as well as the decrease of albumin + globulin polypeptides with a molecular weight over 85.94 kDa and between 85.94-48.00 kDa by about 34 and 8.7%, respectively, were the most notable changes observed in the flour from whole sprouted wheat that clearly point to the intensive protein hydrolysis. The reduction of disulfide bonds and increased concentrations of free -SH groups significantly modify the visco-elastic properties of gliadins and glutenins causing pasting viscosity reduction. However, sprouted wheat flour could be considered as a potential food ingredient because of its improved antioxidant capacity that is a result of protein hydrolysis inter alia. As protein modification induced by steeping may have beneficial effects on the antigenicity of the glutenin fraction, this kind of wheat pretreatment can represent a putative strategy in the dietary modulation of diseases related to glutenin immunoreactivity, e.g. dermatitis herpetiformis.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Effects of enzyme activities during steeping and sprouting on the solubility and composition of proteins, their bioactivity and relationship with the bread making quality of wheat flour",
pages = "4331-4323",
number = "10",
volume = "7",
doi = "10.1039/c6fo01095d"
}

Žilić, S., Janković, M., Barać, M., Pešić, M., Konić-Ristić, A.,& Hadži-Tašković-Šukalović, V.. (2016). Effects of enzyme activities during steeping and sprouting on the solubility and composition of proteins, their bioactivity and relationship with the bread making quality of wheat flour. in Food & Function
Royal Soc Chemistry, Cambridge., 7(10), 4323-4331.
https://doi.org/10.1039/c6fo01095d

Žilić S, Janković M, Barać M, Pešić M, Konić-Ristić A, Hadži-Tašković-Šukalović V. Effects of enzyme activities during steeping and sprouting on the solubility and composition of proteins, their bioactivity and relationship with the bread making quality of wheat flour. in Food & Function. 2016;7(10):4323-4331.
doi:10.1039/c6fo01095d .

Žilić, Sladjana, Janković, Marijana, Barać, Miroljub, Pešić, Mirjana, Konić-Ristić, Aleksandra, Hadži-Tašković-Šukalović, Vesna, "Effects of enzyme activities during steeping and sprouting on the solubility and composition of proteins, their bioactivity and relationship with the bread making quality of wheat flour" in Food & Function, 7, no. 10 (2016):4323-4331,
https://doi.org/10.1039/c6fo01095d . .

TY  - JOUR
AU  - Žilić, Sladjana
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Dodig, Dejan
AU  - Mladenović-Drinić, Snežana
AU  - Janković, Marijana
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2601
AB  - The classical Osborne wheat proteins fraction (albumin-globulin), as well as several polypeptides from the non-gluten protein fraction using SDS-PAGE analyses were determined in the grain of five bread (T. aestivum L.) and five durum wheat (T. durum Desf.) genotypes. In addition, the activity rate of lipoxygenase (LOX) and peroxidase (POD) enzymes implicated in the antioxidant metabolism was determined. Albumins and globulins in wheat grains were characterized by rich protein pattern - the number of bands varied from 19 to 23 and they were defined by molecular weight 76.4-12.4 kDa. The great differences between bread and durum wheat polypeptide contents were found. Result pointed out that polypeptides with molecular weight of 65.6, 43.5 and 32.9 kDa could be used to differentiate the bread from durum wheat. Significant differences in the LOX and POD activity between and within two wheat species were detected. Present study showed in durum wheat a lower activities of LOX and POD enzymes for about 45 and 22%, respectively, than in bread wheat. Our results could be useful for plant breeders in screening and selecting of better raw materials with high protein quality for the flour, breadmaking and pasta industry.
AB  - Određen je sadržaj albuminsko-globulinske frakcije, kao i polipetida koji ulaze u sastav ovih neglutenskih proteina korišćenjem SDS-PAGE analize u zrnu pet genotipova hlebne pšenice (T. aestivum L.) i pet genotipova durum pšenice(T. durum Desf.). Pored toga, određena je aktivnost oksidativnih enzima, lipoksigenaze (LOX) i peroksidaze (POD), koji su uključeni u metabolizam antioksidanasa.Albumisko- globulinska frakcija proteina zrna pšenice karakterisala se bogatom proteinskom šemom - broj traka na gelu je varirao od 19 do 23, a molekulska masa izolovanih proteina kretala se od 76.4 do 12.4 kDa. Detektovani polipeptidi u zrnu genotipova hlebne i durum pšenice razlikovali se u sadržaju. Rezultati ukazuju da bi se polipeptidi molekulskih masa 65.6, 43.5 i 32.9 kDa mogli koristiti za razlikovanje hlebne i durum pšenice. Između i unutar analiziranih vrsta pšenice detektovana je značajna razlika u aktivnosti enzima LOX i POD. Rezultati pokazuju nižu aktivnost LOX i POD u zrnu durum pšenice za 45, odnosno 22% u odnosu na hlebnu pšenicu.Dobijeni rezultati mogu doprineti selekciji osnovnog materijala visokog kvaliteta proteina za upotrebu u pekarstvu i industriji testenina.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes
T1  - Genetička varijabilnost sadržaja albumina i globulina i aktivnosti lipoksigenaze i peroksidaze između genotipova hlebne i durum pšenice
EP  - 516
IS  - 3
SP  - 503
VL  - 43
DO  - 10.2298/GENSR1103503Z
ER  - 

@article{
author = "Žilić, Sladjana and Barać, Miroljub and Pešić, Mirjana and Hadži-Tašković-Šukalović, Vesna and Dodig, Dejan and Mladenović-Drinić, Snežana and Janković, Marijana",
year = "2011",
abstract = "The classical Osborne wheat proteins fraction (albumin-globulin), as well as several polypeptides from the non-gluten protein fraction using SDS-PAGE analyses were determined in the grain of five bread (T. aestivum L.) and five durum wheat (T. durum Desf.) genotypes. In addition, the activity rate of lipoxygenase (LOX) and peroxidase (POD) enzymes implicated in the antioxidant metabolism was determined. Albumins and globulins in wheat grains were characterized by rich protein pattern - the number of bands varied from 19 to 23 and they were defined by molecular weight 76.4-12.4 kDa. The great differences between bread and durum wheat polypeptide contents were found. Result pointed out that polypeptides with molecular weight of 65.6, 43.5 and 32.9 kDa could be used to differentiate the bread from durum wheat. Significant differences in the LOX and POD activity between and within two wheat species were detected. Present study showed in durum wheat a lower activities of LOX and POD enzymes for about 45 and 22%, respectively, than in bread wheat. Our results could be useful for plant breeders in screening and selecting of better raw materials with high protein quality for the flour, breadmaking and pasta industry., Određen je sadržaj albuminsko-globulinske frakcije, kao i polipetida koji ulaze u sastav ovih neglutenskih proteina korišćenjem SDS-PAGE analize u zrnu pet genotipova hlebne pšenice (T. aestivum L.) i pet genotipova durum pšenice(T. durum Desf.). Pored toga, određena je aktivnost oksidativnih enzima, lipoksigenaze (LOX) i peroksidaze (POD), koji su uključeni u metabolizam antioksidanasa.Albumisko- globulinska frakcija proteina zrna pšenice karakterisala se bogatom proteinskom šemom - broj traka na gelu je varirao od 19 do 23, a molekulska masa izolovanih proteina kretala se od 76.4 do 12.4 kDa. Detektovani polipeptidi u zrnu genotipova hlebne i durum pšenice razlikovali se u sadržaju. Rezultati ukazuju da bi se polipeptidi molekulskih masa 65.6, 43.5 i 32.9 kDa mogli koristiti za razlikovanje hlebne i durum pšenice. Između i unutar analiziranih vrsta pšenice detektovana je značajna razlika u aktivnosti enzima LOX i POD. Rezultati pokazuju nižu aktivnost LOX i POD u zrnu durum pšenice za 45, odnosno 22% u odnosu na hlebnu pšenicu.Dobijeni rezultati mogu doprineti selekciji osnovnog materijala visokog kvaliteta proteina za upotrebu u pekarstvu i industriji testenina.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes, Genetička varijabilnost sadržaja albumina i globulina i aktivnosti lipoksigenaze i peroksidaze između genotipova hlebne i durum pšenice",
pages = "516-503",
number = "3",
volume = "43",
doi = "10.2298/GENSR1103503Z"
}

Žilić, S., Barać, M., Pešić, M., Hadži-Tašković-Šukalović, V., Dodig, D., Mladenović-Drinić, S.,& Janković, M.. (2011). Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes. in Genetika
Društvo genetičara Srbije, Beograd., 43(3), 503-516.
https://doi.org/10.2298/GENSR1103503Z

Žilić S, Barać M, Pešić M, Hadži-Tašković-Šukalović V, Dodig D, Mladenović-Drinić S, Janković M. Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes. in Genetika. 2011;43(3):503-516.
doi:10.2298/GENSR1103503Z .

Žilić, Sladjana, Barać, Miroljub, Pešić, Mirjana, Hadži-Tašković-Šukalović, Vesna, Dodig, Dejan, Mladenović-Drinić, Snežana, Janković, Marijana, "Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes" in Genetika, 43, no. 3 (2011):503-516,
https://doi.org/10.2298/GENSR1103503Z . .

TY  - JOUR
AU  - Savić, Sladjana
AU  - Stikić, Radmila
AU  - Vučelić-Radović, Biljana
AU  - Bogicević, Biljana
AU  - Jovanović, Zorica
AU  - Hadži-Tašković-Šukalović, Vesna
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1812
AB  - The effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on tomato fruit growth and cell wall peroxidase activity in tomato exocarp were investigated in growth chamber conditions. The RDI treatment was 50% of water given to fully irrigated (FI) plants and the PRD treatment was 50% of water of FI plants applied to one half of the root system while the other half dried down, with irrigation shifted when soil water content of the dry side decreased 15-20%. RDI significantly reduced fruit diameter, though PRD reduced fresh weight while having no significant effect on fruit diameter. The activity of peroxidase was significantly higher in RDI and PRD treated plants compared to those of FI. Differences between RDI and PRD were expressed on temporal basis. In the fruits of RDI treated plants peroxidase activity began to increase in the phase when fruit growth started to decline with the peak of enzyme activity of 6.1 HRPEU g(-1) FW reached in the phase of mature green fruits when fruit growth rate was minimal. Increase of peroxidase activity in PRD fruits coincided with the ripening phase and the peak of enzyme activity (5.3 HRPEU g(-1) FW) was measured at the end of fruit ripening. These data potentially identified contrasting and different roles of tomato exocarp cell wall peroxidase in RDI and PRD treated plants. In RDI treated plants peroxidase may have a role in restricting fruit growth rate, although the increase in enzyme activity during ripening of PRD treated fruit pointed out that peroxidase may also control fruit maturation by inducing more rapid process.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits
EP  - 20
IS  - 1
SP  - 15
VL  - 117
DO  - 10.1016/j.scienta.2008.03.009
ER  - 

@article{
author = "Savić, Sladjana and Stikić, Radmila and Vučelić-Radović, Biljana and Bogicević, Biljana and Jovanović, Zorica and Hadži-Tašković-Šukalović, Vesna",
year = "2008",
abstract = "The effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on tomato fruit growth and cell wall peroxidase activity in tomato exocarp were investigated in growth chamber conditions. The RDI treatment was 50% of water given to fully irrigated (FI) plants and the PRD treatment was 50% of water of FI plants applied to one half of the root system while the other half dried down, with irrigation shifted when soil water content of the dry side decreased 15-20%. RDI significantly reduced fruit diameter, though PRD reduced fresh weight while having no significant effect on fruit diameter. The activity of peroxidase was significantly higher in RDI and PRD treated plants compared to those of FI. Differences between RDI and PRD were expressed on temporal basis. In the fruits of RDI treated plants peroxidase activity began to increase in the phase when fruit growth started to decline with the peak of enzyme activity of 6.1 HRPEU g(-1) FW reached in the phase of mature green fruits when fruit growth rate was minimal. Increase of peroxidase activity in PRD fruits coincided with the ripening phase and the peak of enzyme activity (5.3 HRPEU g(-1) FW) was measured at the end of fruit ripening. These data potentially identified contrasting and different roles of tomato exocarp cell wall peroxidase in RDI and PRD treated plants. In RDI treated plants peroxidase may have a role in restricting fruit growth rate, although the increase in enzyme activity during ripening of PRD treated fruit pointed out that peroxidase may also control fruit maturation by inducing more rapid process.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits",
pages = "20-15",
number = "1",
volume = "117",
doi = "10.1016/j.scienta.2008.03.009"
}

Savić, S., Stikić, R., Vučelić-Radović, B., Bogicević, B., Jovanović, Z.,& Hadži-Tašković-Šukalović, V.. (2008). Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits. in Scientia Horticulturae
Elsevier, Amsterdam., 117(1), 15-20.
https://doi.org/10.1016/j.scienta.2008.03.009

Savić S, Stikić R, Vučelić-Radović B, Bogicević B, Jovanović Z, Hadži-Tašković-Šukalović V. Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits. in Scientia Horticulturae. 2008;117(1):15-20.
doi:10.1016/j.scienta.2008.03.009 .

Savić, Sladjana, Stikić, Radmila, Vučelić-Radović, Biljana, Bogicević, Biljana, Jovanović, Zorica, Hadži-Tašković-Šukalović, Vesna, "Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits" in Scientia Horticulturae, 117, no. 1 (2008):15-20,
https://doi.org/10.1016/j.scienta.2008.03.009 . .