TY  - JOUR
AU  - Sipsas, Vasilios
AU  - Kolokythas, George
AU  - Kourkoutas, Yiannis
AU  - Plessas, Stavros
AU  - Nedović, Viktor
AU  - Kanellaki, Maria
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2050
AB  - A freeze-dried immobilized biocatalyst produced by immobilization of Saccharomyces cerevisiae AXAZ-1 yeast cells on gluten pellets and subsequent freeze-drying was used in a multistage fixed-bed tower (MFBT) bioreactor for batch and continuous wine-making. The MFBT bioreactor resulted in higher alcohol productivity compared to fermentations carried out in a packed bed (PB) bioreactor and showed an important operational stability and no decrease in activity, even at low fermentation temperature (5 degrees C) and after storage for 6 months at 4 degrees C. The production of amyl alcohols proved to be temperature dependent and was significantly reduced at low temperatures. Re-activation of the freeze-dried immobilized cells after storage for 6 months resulted in further decreased content of amyl alcohols. The SPME GC/MS analysis of volatile compounds revealed no significant differences in the wines produced by MFBT and PB bioreactors, while the preliminary sensory evaluation ascertained the overall improved quality of the produced wines. Potential industrial application of MFBT bioreactor is also assessed and discussed.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Food Engineering
T1  - Comparative study of batch and continuous multi-stage fixed-bed tower (MFBT) bioreactor during wine-making using freeze-dried immobilized cells
EP  - 503
IS  - 4
SP  - 495
VL  - 90
DO  - 10.1016/j.jfoodeng.2008.07.016
ER  - 

@article{
author = "Sipsas, Vasilios and Kolokythas, George and Kourkoutas, Yiannis and Plessas, Stavros and Nedović, Viktor and Kanellaki, Maria",
year = "2009",
abstract = "A freeze-dried immobilized biocatalyst produced by immobilization of Saccharomyces cerevisiae AXAZ-1 yeast cells on gluten pellets and subsequent freeze-drying was used in a multistage fixed-bed tower (MFBT) bioreactor for batch and continuous wine-making. The MFBT bioreactor resulted in higher alcohol productivity compared to fermentations carried out in a packed bed (PB) bioreactor and showed an important operational stability and no decrease in activity, even at low fermentation temperature (5 degrees C) and after storage for 6 months at 4 degrees C. The production of amyl alcohols proved to be temperature dependent and was significantly reduced at low temperatures. Re-activation of the freeze-dried immobilized cells after storage for 6 months resulted in further decreased content of amyl alcohols. The SPME GC/MS analysis of volatile compounds revealed no significant differences in the wines produced by MFBT and PB bioreactors, while the preliminary sensory evaluation ascertained the overall improved quality of the produced wines. Potential industrial application of MFBT bioreactor is also assessed and discussed.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Food Engineering",
title = "Comparative study of batch and continuous multi-stage fixed-bed tower (MFBT) bioreactor during wine-making using freeze-dried immobilized cells",
pages = "503-495",
number = "4",
volume = "90",
doi = "10.1016/j.jfoodeng.2008.07.016"
}

Sipsas, V., Kolokythas, G., Kourkoutas, Y., Plessas, S., Nedović, V.,& Kanellaki, M.. (2009). Comparative study of batch and continuous multi-stage fixed-bed tower (MFBT) bioreactor during wine-making using freeze-dried immobilized cells. in Journal of Food Engineering
Elsevier Sci Ltd, Oxford., 90(4), 495-503.
https://doi.org/10.1016/j.jfoodeng.2008.07.016

Sipsas V, Kolokythas G, Kourkoutas Y, Plessas S, Nedović V, Kanellaki M. Comparative study of batch and continuous multi-stage fixed-bed tower (MFBT) bioreactor during wine-making using freeze-dried immobilized cells. in Journal of Food Engineering. 2009;90(4):495-503.
doi:10.1016/j.jfoodeng.2008.07.016 .

Sipsas, Vasilios, Kolokythas, George, Kourkoutas, Yiannis, Plessas, Stavros, Nedović, Viktor, Kanellaki, Maria, "Comparative study of batch and continuous multi-stage fixed-bed tower (MFBT) bioreactor during wine-making using freeze-dried immobilized cells" in Journal of Food Engineering, 90, no. 4 (2009):495-503,
https://doi.org/10.1016/j.jfoodeng.2008.07.016 . .

TY  - CONF
AU  - Manojlović, Verica
AU  - Agouridis, Nikos
AU  - Kopsahelis, Nikos
AU  - Kanellaki, Maria
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1640
AB  - The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of ethanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.
PB  - 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
C3  - Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
T1  - Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor
EP  - 334
SP  - 327
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1640
ER  - 

@conference{
author = "Manojlović, Verica and Agouridis, Nikos and Kopsahelis, Nikos and Kanellaki, Maria and Bugarski, Branko and Nedović, Viktor",
year = "2008",
abstract = "The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of ethanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.",
publisher = "2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists",
journal = "Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20",
title = "Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor",
pages = "334-327",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1640"
}

Manojlović, V., Agouridis, N., Kopsahelis, N., Kanellaki, M., Bugarski, B.,& Nedović, V.. (2008). Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists., 2, 327-334.
https://hdl.handle.net/21.15107/rcub_agrospace_1640

Manojlović V, Agouridis N, Kopsahelis N, Kanellaki M, Bugarski B, Nedović V. Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20. 2008;2:327-334.
https://hdl.handle.net/21.15107/rcub_agrospace_1640 .

Manojlović, Verica, Agouridis, Nikos, Kopsahelis, Nikos, Kanellaki, Maria, Bugarski, Branko, Nedović, Viktor, "Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor" in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2 (2008):327-334,
https://hdl.handle.net/21.15107/rcub_agrospace_1640 .