Milijašević, Milan

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  • Milijašević, Milan (3)
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Author's Bibliography

Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese

Vesković-Moračanin, Slavica; Đukić, Dragutin; Velebit, Branko; Borović, Branka; Milijašević, Milan; Stojanova, Monika

(SAGE Publications Inc., 2022)

TY  - JOUR
AU  - Vesković-Moračanin, Slavica
AU  - Đukić, Dragutin
AU  - Velebit, Branko
AU  - Borović, Branka
AU  - Milijašević, Milan
AU  - Stojanova, Monika
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6097
AB  - The aim of this study was to select autochthonous lactic acid bacteria (LAB) isolates with antilisterial activity from Zlatar cheese and to evaluate the ability of selected LAB to control Listeria monocytogenes growth during soft white cheese production. The genotype characterization of isolated LAB (n = 93) was done using PCR method by 16S rRNA sequencing. In this way, the following isolates were detected: Lactococcus lactis ssp. lactis (40 isolates), Enterococcus faecalis (30), Lactobacillus plantarum (12), Leuconostoc mesenteroides ssp. mesenteroides (3) Lactobacillus garvieae (3), Lactobacillus curvatus (2), Lactobacillus casei (1), Enterococcus faecium (1) and Staphylococcus hominis (1). Each isolated LAB was tested for bacteriocin-producing ability. It was determined that two LAB isolates had bactericidal properties: Lactococcus lactis ssp. lactis SRB/ZS/094 and Enterococcus faecalis SRB/ZS/090. Semi-purified of enterococcal bacteriocin (enterocin) was isolated using precipitation procedures with ammonium sulphate. Its properties were determined (strength and range of activities). Isolated enterocin and bacteriocin-producing Lactococcus strain showed significant antimicribial activity against Listeria monocytogenes, but still the inhibition activity of Staphylococcus aurues and Escherichia coli was not detected. Based on the obtained laboratory results, in the second phase of the research, the antilisterial effect of bacteriocin isolated from Enterococcus faecalis SRB/ZS/090 and cells Lactococcus lactis ssp. lactis SRB/ZS/094 were determined, that are added as additives in the production of soft white cheese through five variants. Cheese supplemented with enterocin (E2) had the lowest aerobic mesophilic bacteria count, indicating that enterocin (E2) play an important role for bio-preservation.
PB  - SAGE Publications Inc.
T2  - Food Science and Technology International
T1  - Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese
DO  - 10.1177/10820132221097871
ER  - 
@article{
author = "Vesković-Moračanin, Slavica and Đukić, Dragutin and Velebit, Branko and Borović, Branka and Milijašević, Milan and Stojanova, Monika",
year = "2022",
abstract = "The aim of this study was to select autochthonous lactic acid bacteria (LAB) isolates with antilisterial activity from Zlatar cheese and to evaluate the ability of selected LAB to control Listeria monocytogenes growth during soft white cheese production. The genotype characterization of isolated LAB (n = 93) was done using PCR method by 16S rRNA sequencing. In this way, the following isolates were detected: Lactococcus lactis ssp. lactis (40 isolates), Enterococcus faecalis (30), Lactobacillus plantarum (12), Leuconostoc mesenteroides ssp. mesenteroides (3) Lactobacillus garvieae (3), Lactobacillus curvatus (2), Lactobacillus casei (1), Enterococcus faecium (1) and Staphylococcus hominis (1). Each isolated LAB was tested for bacteriocin-producing ability. It was determined that two LAB isolates had bactericidal properties: Lactococcus lactis ssp. lactis SRB/ZS/094 and Enterococcus faecalis SRB/ZS/090. Semi-purified of enterococcal bacteriocin (enterocin) was isolated using precipitation procedures with ammonium sulphate. Its properties were determined (strength and range of activities). Isolated enterocin and bacteriocin-producing Lactococcus strain showed significant antimicribial activity against Listeria monocytogenes, but still the inhibition activity of Staphylococcus aurues and Escherichia coli was not detected. Based on the obtained laboratory results, in the second phase of the research, the antilisterial effect of bacteriocin isolated from Enterococcus faecalis SRB/ZS/090 and cells Lactococcus lactis ssp. lactis SRB/ZS/094 were determined, that are added as additives in the production of soft white cheese through five variants. Cheese supplemented with enterocin (E2) had the lowest aerobic mesophilic bacteria count, indicating that enterocin (E2) play an important role for bio-preservation.",
publisher = "SAGE Publications Inc.",
journal = "Food Science and Technology International",
title = "Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese",
doi = "10.1177/10820132221097871"
}
Vesković-Moračanin, S., Đukić, D., Velebit, B., Borović, B., Milijašević, M.,& Stojanova, M.. (2022). Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese. in Food Science and Technology International
SAGE Publications Inc...
https://doi.org/10.1177/10820132221097871
Vesković-Moračanin S, Đukić D, Velebit B, Borović B, Milijašević M, Stojanova M. Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese. in Food Science and Technology International. 2022;.
doi:10.1177/10820132221097871 .
Vesković-Moračanin, Slavica, Đukić, Dragutin, Velebit, Branko, Borović, Branka, Milijašević, Milan, Stojanova, Monika, "Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese" in Food Science and Technology International (2022),
https://doi.org/10.1177/10820132221097871 . .
1

Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese

Vesković-Moračanin, Slavica; Dragutin, Đukić; Velebit, Branko; Borović, Branka; Milijašević, Milan; Stojanova, Monika

(2022)

TY  - JOUR
AU  - Vesković-Moračanin, Slavica
AU  - Dragutin, Đukić
AU  - Velebit, Branko
AU  - Borović, Branka
AU  - Milijašević, Milan
AU  - Stojanova, Monika
PY  - 2022
UR  - https://doi.org/10.1177/10820132221097871
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6100
AB  - The aim of this study was to select autochthonous lactic acid bacteria (LAB) isolates with antilisterial activity from Zlatar cheese and to evaluate the ability of selected LAB to control Listeria monocytogenes growth during soft white cheese production. The genotype characterization of isolated LAB (n = 93) was done using PCR method by 16S rRNA sequencing. In this way, the following isolates were detected: Lactococcus lactis ssp. lactis (40 isolates), Enterococcus faecalis (30), Lactobacillus plantarum (12), Leuconostoc mesenteroides ssp. mesenteroides (3) Lactobacillus garvieae (3), Lactobacillus curvatus (2), Lactobacillus casei (1), Enterococcus faecium (1) and Staphylococcus hominis (1). Each isolated LAB was tested for bacteriocin-producing ability. It was determined that two LAB isolates had bactericidal properties: Lactococcus lactis ssp. lactis SRB/ZS/094 and Enterococcus faecalis SRB/ZS/090. Semi-purified of enterococcal bacteriocin (enterocin) was isolated using precipitation procedures with ammonium sulphate. Its properties were determined (strength and range of activities). Isolated enterocin and bacteriocin-producing Lactococcus strain showed significant antimicribial activity against Listeria monocytogenes, but still the inhibition activity of Staphylococcus aurues and Escherichia coli was not detected. Based on the obtained laboratory results, in the second phase of the research, the antilisterial effect of bacteriocin isolated from Enterococcus faecalis SRB/ZS/090 and cells Lactococcus lactis ssp. lactis SRB/ZS/094 were determined, that are added as additives in the production of soft white cheese through five variants. Cheese supplemented with enterocin (E2) had the lowest aerobic mesophilic bacteria count, indicating that enterocin (E2) play an important role for bio-preservation.
T2  - Food Science and Technology International
T2  - Food Science and Technology InternationalFood sci. technol. int.
T1  - Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese
SP  - 10820132221097871
DO  - 10.1177/10820132221097871
ER  - 
@article{
author = "Vesković-Moračanin, Slavica and Dragutin, Đukić and Velebit, Branko and Borović, Branka and Milijašević, Milan and Stojanova, Monika",
year = "2022",
abstract = "The aim of this study was to select autochthonous lactic acid bacteria (LAB) isolates with antilisterial activity from Zlatar cheese and to evaluate the ability of selected LAB to control Listeria monocytogenes growth during soft white cheese production. The genotype characterization of isolated LAB (n = 93) was done using PCR method by 16S rRNA sequencing. In this way, the following isolates were detected: Lactococcus lactis ssp. lactis (40 isolates), Enterococcus faecalis (30), Lactobacillus plantarum (12), Leuconostoc mesenteroides ssp. mesenteroides (3) Lactobacillus garvieae (3), Lactobacillus curvatus (2), Lactobacillus casei (1), Enterococcus faecium (1) and Staphylococcus hominis (1). Each isolated LAB was tested for bacteriocin-producing ability. It was determined that two LAB isolates had bactericidal properties: Lactococcus lactis ssp. lactis SRB/ZS/094 and Enterococcus faecalis SRB/ZS/090. Semi-purified of enterococcal bacteriocin (enterocin) was isolated using precipitation procedures with ammonium sulphate. Its properties were determined (strength and range of activities). Isolated enterocin and bacteriocin-producing Lactococcus strain showed significant antimicribial activity against Listeria monocytogenes, but still the inhibition activity of Staphylococcus aurues and Escherichia coli was not detected. Based on the obtained laboratory results, in the second phase of the research, the antilisterial effect of bacteriocin isolated from Enterococcus faecalis SRB/ZS/090 and cells Lactococcus lactis ssp. lactis SRB/ZS/094 were determined, that are added as additives in the production of soft white cheese through five variants. Cheese supplemented with enterocin (E2) had the lowest aerobic mesophilic bacteria count, indicating that enterocin (E2) play an important role for bio-preservation.",
journal = "Food Science and Technology International, Food Science and Technology InternationalFood sci. technol. int.",
title = "Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese",
pages = "10820132221097871",
doi = "10.1177/10820132221097871"
}
Vesković-Moračanin, S., Dragutin, Đ., Velebit, B., Borović, B., Milijašević, M.,& Stojanova, M.. (2022). Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese. in Food Science and Technology International, 10820132221097871.
https://doi.org/10.1177/10820132221097871
Vesković-Moračanin S, Dragutin Đ, Velebit B, Borović B, Milijašević M, Stojanova M. Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese. in Food Science and Technology International. 2022;:10820132221097871.
doi:10.1177/10820132221097871 .
Vesković-Moračanin, Slavica, Dragutin, Đukić, Velebit, Branko, Borović, Branka, Milijašević, Milan, Stojanova, Monika, "Determination of antilisterial effect of some microbial isolates from traditional zlatar cheese during the fermentation of soft white cheese" in Food Science and Technology International (2022):10820132221097871,
https://doi.org/10.1177/10820132221097871 . .
1

Changes in the proximate and fatty acid composition in carp meat during the semi intensive farming

Trbović, Dejana; Marković, Zoran; Petronijević, Radivoj; Milijašević, Milan; Spirić, Danka; Vranić, Danijela; Spirić, Aurelija

(Institut za higijenu i tehnologiju mesa, Beograd, 2013)

TY  - JOUR
AU  - Trbović, Dejana
AU  - Marković, Zoran
AU  - Petronijević, Radivoj
AU  - Milijašević, Milan
AU  - Spirić, Danka
AU  - Vranić, Danijela
AU  - Spirić, Aurelija
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3215
AB  - The aim of this study was to examine and evaluate the proximate composition and fatty acid profiles of carp (Cyprinus carpio) during rearing in the semi-intensive farming conditions, supplementary fed extruded feed. Carp at the age of two years was submitted to trials, from spring to autumn, at the fish farm 'Ečka' AD. Samples of carp were collected in April, June, September and October. The protein content in fish sampled in September was significantly different from the protein content in fish sampled in April, June and October (p  lt  0.001), (17.48%, 17.27%, 18.28% and 17.26%, respectively). The quantities of total lipids slightly increased (2.25%, 2.37%, 3.02% and 4.72%, respectively) with the increase of the fish weight (598 g, 874 g, 1439 g and 1984 g, respectively), but significant increases occurred between September and October (p  lt  0.001). The moisture content decreased (79.55%, 78.86%, 77.46% and 75.72%, respectively). Principal Component Analysis (PCA) and Linear Discrimination Analysis (LDA) indicated that there were significant changes in the fatty acid composition of carp during growth. Starting from April to October the quantities of fatty acids were as follows: SFA (saturated fatty acids) - 28.47%, 28.97%, 24.86% and 23.66%, respectively; MUFA (monounsaturated fatty acids) - 38.57%, 40.52%, 41.68% and 42.43%, respectively; PUFA (polyunsaturated fatty acids) - 32.53%, 30.49%, 31.53% and 32.55%, respectively. The additional feeding of carp with the extruded feed influenced the increase in quantities of MUFA and n-6 PUFA (24.98%, 22.86%, 26.96% and 27.99%, respectively), and the decrease in quantities of the nutritionally important n-3 PUFA (5.13%, 6.59%, 4.57% and 4.57%, respectively). The highest n-3/n-6 ratio was obtained in June (0.30) and the lowest in October (0.16), indicating that the applied extruded feed was rich in n-6 and poor in n-3 PUFA. PCA and LDA have shown that significant changes in the fatty acid composition of carp during the breeding occurred. Separation of the carp according to the sampling period was achieved by the LDA analysis, which is consistent with the type of ingested food.
AB  - Cilj ovog rada bio je ispitivanje osnovnog hemijskog i masnokiselinskog sastava šarana (Cyprinus carpio) u toku uzgoja u poluintenzivnom sistemu uz prihranjivanje ribe ekstrudiranom hranom, kao i statistička evaluacija dobijenih rezultata. Eksperiment je realizovan od proleća do jeseni, na ribarskom gazdinstvu 'Ečka'AD, a korišćen je dvogodišnji šaran u nasadu za dvogodišnji. Uzorci šarana su uzimani u toku aprila, juna, septembra i oktobra meseca. Sadržaj proteina u šaranu koji je uzorkovan u septembru značajno se razlikovao od sadržaja proteina u šaranu koji je uzorkovan u aprilu, junu i oktobru (p  lt  0,001), (17,48%, 17,27%, 18,28% i 17,26%, respektivno). Količine ukupnih lipida su blago rasle (2,25%, 2,37%, 3,02% i 4,72%, respektivno) sa povećanjem mase ribe (598 g, 874 g, 1439 g i 1984 g, respektivno), a između septembra i oktobra došlo je do značajnog povećanja ukupnih lipida (p  lt 0,001). Sadržaj vlage se smanjivao (79,55%, 78,86%, 77,46% i 75,72%, respektivno). Analiza glavnih komponenti (Principal Component Analysis, PCA) i diskriminaciona linearna analiza (Linear Discrimination Analysis, LDA) ukazuju da je u toku perioda rasta ribe došlo do značajnih promena u sastavu masnih kiselina. U periodu istraživanja, od aprila do oktobra, količine masnih kiselina su bile sledeće: ZMK (zasićene masne kiseline) - 28,47%, 28,97%, 24,86% i 23,66%, respektivno; MNMK (mononezasićene masne kiseline) - 38,57%, 40,52%, 41,68% i 42,43%, respektivno, PNMK (polinezasićene masne kiseline) - 32,.53% , 30,49, 31,53% i 32,55%%, respektivno. Prihranjivanje šarana ekstrudiranom hranom uticalo je na porast količina MNMK i n-6 PNMK (24,98%, 22,86%, 26,96% i 27,99%, respektivno), kao i na smanjenje količina nutritivno važnih n-3 PNMK (5,13, 6,59%%, 4,57% i 4,57%, respektivno). Najveći odnos n-3/n-6 masnih kiselina dobijen je u junu (0,30), a najmanji u oktobru (0,16), što ukazuje da je ekstrudirana hrana koja je na ribnjaku korišćena bila bogata sa n-6 i siromašna sa n-3 PNMK. PCA i LDA su pokazale da je došlo do značajnih promena u sastavu masnih kiselina šarana tokom uzgoja. LDA analizom postignuto je razdvajanje šarana prema periodu uzorkovanja, a što je u korelaciji sa vrstom unete hrane.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Changes in the proximate and fatty acid composition in carp meat during the semi intensive farming
T1  - Promene hemijskog i masnokiselinskog sastava mesa šarana u toku poluintenzivnog uzgoja
EP  - 47
IS  - 1
SP  - 39
VL  - 54
DO  - 10.5937/tehmesa1301039T
ER  - 
@article{
author = "Trbović, Dejana and Marković, Zoran and Petronijević, Radivoj and Milijašević, Milan and Spirić, Danka and Vranić, Danijela and Spirić, Aurelija",
year = "2013",
abstract = "The aim of this study was to examine and evaluate the proximate composition and fatty acid profiles of carp (Cyprinus carpio) during rearing in the semi-intensive farming conditions, supplementary fed extruded feed. Carp at the age of two years was submitted to trials, from spring to autumn, at the fish farm 'Ečka' AD. Samples of carp were collected in April, June, September and October. The protein content in fish sampled in September was significantly different from the protein content in fish sampled in April, June and October (p  lt  0.001), (17.48%, 17.27%, 18.28% and 17.26%, respectively). The quantities of total lipids slightly increased (2.25%, 2.37%, 3.02% and 4.72%, respectively) with the increase of the fish weight (598 g, 874 g, 1439 g and 1984 g, respectively), but significant increases occurred between September and October (p  lt  0.001). The moisture content decreased (79.55%, 78.86%, 77.46% and 75.72%, respectively). Principal Component Analysis (PCA) and Linear Discrimination Analysis (LDA) indicated that there were significant changes in the fatty acid composition of carp during growth. Starting from April to October the quantities of fatty acids were as follows: SFA (saturated fatty acids) - 28.47%, 28.97%, 24.86% and 23.66%, respectively; MUFA (monounsaturated fatty acids) - 38.57%, 40.52%, 41.68% and 42.43%, respectively; PUFA (polyunsaturated fatty acids) - 32.53%, 30.49%, 31.53% and 32.55%, respectively. The additional feeding of carp with the extruded feed influenced the increase in quantities of MUFA and n-6 PUFA (24.98%, 22.86%, 26.96% and 27.99%, respectively), and the decrease in quantities of the nutritionally important n-3 PUFA (5.13%, 6.59%, 4.57% and 4.57%, respectively). The highest n-3/n-6 ratio was obtained in June (0.30) and the lowest in October (0.16), indicating that the applied extruded feed was rich in n-6 and poor in n-3 PUFA. PCA and LDA have shown that significant changes in the fatty acid composition of carp during the breeding occurred. Separation of the carp according to the sampling period was achieved by the LDA analysis, which is consistent with the type of ingested food., Cilj ovog rada bio je ispitivanje osnovnog hemijskog i masnokiselinskog sastava šarana (Cyprinus carpio) u toku uzgoja u poluintenzivnom sistemu uz prihranjivanje ribe ekstrudiranom hranom, kao i statistička evaluacija dobijenih rezultata. Eksperiment je realizovan od proleća do jeseni, na ribarskom gazdinstvu 'Ečka'AD, a korišćen je dvogodišnji šaran u nasadu za dvogodišnji. Uzorci šarana su uzimani u toku aprila, juna, septembra i oktobra meseca. Sadržaj proteina u šaranu koji je uzorkovan u septembru značajno se razlikovao od sadržaja proteina u šaranu koji je uzorkovan u aprilu, junu i oktobru (p  lt  0,001), (17,48%, 17,27%, 18,28% i 17,26%, respektivno). Količine ukupnih lipida su blago rasle (2,25%, 2,37%, 3,02% i 4,72%, respektivno) sa povećanjem mase ribe (598 g, 874 g, 1439 g i 1984 g, respektivno), a između septembra i oktobra došlo je do značajnog povećanja ukupnih lipida (p  lt 0,001). Sadržaj vlage se smanjivao (79,55%, 78,86%, 77,46% i 75,72%, respektivno). Analiza glavnih komponenti (Principal Component Analysis, PCA) i diskriminaciona linearna analiza (Linear Discrimination Analysis, LDA) ukazuju da je u toku perioda rasta ribe došlo do značajnih promena u sastavu masnih kiselina. U periodu istraživanja, od aprila do oktobra, količine masnih kiselina su bile sledeće: ZMK (zasićene masne kiseline) - 28,47%, 28,97%, 24,86% i 23,66%, respektivno; MNMK (mononezasićene masne kiseline) - 38,57%, 40,52%, 41,68% i 42,43%, respektivno, PNMK (polinezasićene masne kiseline) - 32,.53% , 30,49, 31,53% i 32,55%%, respektivno. Prihranjivanje šarana ekstrudiranom hranom uticalo je na porast količina MNMK i n-6 PNMK (24,98%, 22,86%, 26,96% i 27,99%, respektivno), kao i na smanjenje količina nutritivno važnih n-3 PNMK (5,13, 6,59%%, 4,57% i 4,57%, respektivno). Najveći odnos n-3/n-6 masnih kiselina dobijen je u junu (0,30), a najmanji u oktobru (0,16), što ukazuje da je ekstrudirana hrana koja je na ribnjaku korišćena bila bogata sa n-6 i siromašna sa n-3 PNMK. PCA i LDA su pokazale da je došlo do značajnih promena u sastavu masnih kiselina šarana tokom uzgoja. LDA analizom postignuto je razdvajanje šarana prema periodu uzorkovanja, a što je u korelaciji sa vrstom unete hrane.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Changes in the proximate and fatty acid composition in carp meat during the semi intensive farming, Promene hemijskog i masnokiselinskog sastava mesa šarana u toku poluintenzivnog uzgoja",
pages = "47-39",
number = "1",
volume = "54",
doi = "10.5937/tehmesa1301039T"
}
Trbović, D., Marković, Z., Petronijević, R., Milijašević, M., Spirić, D., Vranić, D.,& Spirić, A.. (2013). Changes in the proximate and fatty acid composition in carp meat during the semi intensive farming. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 54(1), 39-47.
https://doi.org/10.5937/tehmesa1301039T
Trbović D, Marković Z, Petronijević R, Milijašević M, Spirić D, Vranić D, Spirić A. Changes in the proximate and fatty acid composition in carp meat during the semi intensive farming. in Tehnologija mesa. 2013;54(1):39-47.
doi:10.5937/tehmesa1301039T .
Trbović, Dejana, Marković, Zoran, Petronijević, Radivoj, Milijašević, Milan, Spirić, Danka, Vranić, Danijela, Spirić, Aurelija, "Changes in the proximate and fatty acid composition in carp meat during the semi intensive farming" in Tehnologija mesa, 54, no. 1 (2013):39-47,
https://doi.org/10.5937/tehmesa1301039T . .
4