TY  - JOUR
AU  - Milićević, Zoran
AU  - Krnjajić, Slobodan
AU  - Stević, Milan
AU  - Ćirković, Jovana
AU  - Jelušić, Aleksandra
AU  - Pucarević, Mira
AU  - Popović, Tatjana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6045
AB  - In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.
PB  - MDPI
T2  - Agriculture (Switzerland)
T1  - Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide
IS  - 3
SP  - 338
VL  - 12
DO  - 10.3390/agriculture12030338
ER  - 

@article{
author = "Milićević, Zoran and Krnjajić, Slobodan and Stević, Milan and Ćirković, Jovana and Jelušić, Aleksandra and Pucarević, Mira and Popović, Tatjana",
year = "2022",
abstract = "In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.",
publisher = "MDPI",
journal = "Agriculture (Switzerland)",
title = "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide",
number = "3",
pages = "338",
volume = "12",
doi = "10.3390/agriculture12030338"
}

Milićević, Z., Krnjajić, S., Stević, M., Ćirković, J., Jelušić, A., Pucarević, M.,& Popović, T.. (2022). Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland)
MDPI., 12(3), 338.
https://doi.org/10.3390/agriculture12030338

Milićević Z, Krnjajić S, Stević M, Ćirković J, Jelušić A, Pucarević M, Popović T. Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland). 2022;12(3):338.
doi:10.3390/agriculture12030338 .

Milićević, Zoran, Krnjajić, Slobodan, Stević, Milan, Ćirković, Jovana, Jelušić, Aleksandra, Pucarević, Mira, Popović, Tatjana, "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide" in Agriculture (Switzerland), 12, no. 3 (2022):338,
https://doi.org/10.3390/agriculture12030338 . .

TY  - JOUR
AU  - Jevremović, Stojan
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Krnjajić, Slobodan
AU  - Ugrenović, Vladan
AU  - Radonjić, Andja
AU  - Kostić, Igor
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4960
AB  - The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans
EP  - 676
IS  - 4
SP  - 665
VL  - 71
DO  - 10.2298/ABS190617049J
ER  - 

@article{
author = "Jevremović, Stojan and Lazarević, Jelica and Kostić, Miroslav and Krnjajić, Slobodan and Ugrenović, Vladan and Radonjić, Andja and Kostić, Igor",
year = "2019",
abstract = "The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans",
pages = "676-665",
number = "4",
volume = "71",
doi = "10.2298/ABS190617049J"
}

Jevremović, S., Lazarević, J., Kostić, M., Krnjajić, S., Ugrenović, V., Radonjić, A.,& Kostić, I.. (2019). Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(4), 665-676.
https://doi.org/10.2298/ABS190617049J

Jevremović S, Lazarević J, Kostić M, Krnjajić S, Ugrenović V, Radonjić A, Kostić I. Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences. 2019;71(4):665-676.
doi:10.2298/ABS190617049J .

Jevremović, Stojan, Lazarević, Jelica, Kostić, Miroslav, Krnjajić, Slobodan, Ugrenović, Vladan, Radonjić, Andja, Kostić, Igor, "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans" in Archives of Biological Sciences, 71, no. 4 (2019):665-676,
https://doi.org/10.2298/ABS190617049J . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Berenji, Janoš
AU  - Krnjajić, Slobodan
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2936
AB  - Sixteen samples of sorghum seed (Sorghum bicolor (L.) Moench.) 'Alba', 'Gold', 'Prima' and 'Reform' were analyzed in the localities of Bački Petrovac and Čantavir in the period 2009-2011. Tipresence of species belonging to the genera Epicoccum, Fusarium, Alternaria, Aspergillus and Penicillium was established in single and mixed infections. From the infected sorghum seed, monosporial cultures identified as Epicoccum nigrum based on morphology, proved their pathogenicity on artificially inoculated sorghum seedlings. Molecular identification was performed by PCR and amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates 291-09 (JQ619838) and 315-09 (JQ619839) exhibited 99-100% nucleotide identity with the sequences of 31 isolates of E. nigrum deposited in the GenBank. It obtained results represent the first detailed characterization of E. nigrum in Serbia. The presence of a large number of phytopathogenic fungi on sorghum seed should be further investigated in order to clarify their relationships and relative significance.
AB  - U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Epicoccum nigrum the new pathogen of sorghum seed in Serbia
T1  - Epicoccum nigrum novi patogen semena sirka u Srbiji
EP  - 166
IS  - 2
SP  - 160
VL  - 49
DO  - 10.5937/ratpov49-1793
ER  - 

@article{
author = "Ristić, Danijela and Stanković, Ivana and Vučurović, Ana and Berenji, Janoš and Krnjajić, Slobodan and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
abstract = "Sixteen samples of sorghum seed (Sorghum bicolor (L.) Moench.) 'Alba', 'Gold', 'Prima' and 'Reform' were analyzed in the localities of Bački Petrovac and Čantavir in the period 2009-2011. Tipresence of species belonging to the genera Epicoccum, Fusarium, Alternaria, Aspergillus and Penicillium was established in single and mixed infections. From the infected sorghum seed, monosporial cultures identified as Epicoccum nigrum based on morphology, proved their pathogenicity on artificially inoculated sorghum seedlings. Molecular identification was performed by PCR and amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates 291-09 (JQ619838) and 315-09 (JQ619839) exhibited 99-100% nucleotide identity with the sequences of 31 isolates of E. nigrum deposited in the GenBank. It obtained results represent the first detailed characterization of E. nigrum in Serbia. The presence of a large number of phytopathogenic fungi on sorghum seed should be further investigated in order to clarify their relationships and relative significance., U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Epicoccum nigrum the new pathogen of sorghum seed in Serbia, Epicoccum nigrum novi patogen semena sirka u Srbiji",
pages = "166-160",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1793"
}

Ristić, D., Stanković, I., Vučurović, A., Berenji, J., Krnjajić, S., Krstić, B.,& Bulajić, A.. (2012). Epicoccum nigrum the new pathogen of sorghum seed in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 160-166.
https://doi.org/10.5937/ratpov49-1793

Ristić D, Stanković I, Vučurović A, Berenji J, Krnjajić S, Krstić B, Bulajić A. Epicoccum nigrum the new pathogen of sorghum seed in Serbia. in Ratarstvo i povrtarstvo. 2012;49(2):160-166.
doi:10.5937/ratpov49-1793 .

Ristić, Danijela, Stanković, Ivana, Vučurović, Ana, Berenji, Janoš, Krnjajić, Slobodan, Krstić, Branka, Bulajić, Aleksandra, "Epicoccum nigrum the new pathogen of sorghum seed in Serbia" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):160-166,
https://doi.org/10.5937/ratpov49-1793 . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2314
AB  - In a survey to determine the presence of Phytophthora ramorum in Serbia, ornamentals from garden centers, nurseries, and private and public gardens, as well as imported plant material, were inspected. In total, 577 plant, soil, and potting media samples were tested using various detection methods: lateral flow diagnostic test, enzyme-linked immunosorbent assay, conventional polymerase chain reaction, and isolation, followed by identification based on growth characteristics in culture and morphological features. P. ramorum was not detected in any of the 162 soil or potting media tested by the baiting method. P. ramorum was detected in 12 Rhododendron samples from one private garden in Zemun (City of Belgrade District) exhibiting symptoms of leaf necrosis and blight and petiole necrosis, and in three samples of Pieris spp. from one garden center exhibiting symptoms of leaf necrosis. Eight Phytophthora isolates were obtained from the positive Rhododendron plants and three isolates from Pieris plants, and all were identified as P. ramorum on the basis of their uniform morphological and growth characteristics. P. ramorum conformation was also made by sequencing of the internal transcribed spacer regions for a single isolate taken from one infected rhododendron and one pieris plant. Serbian isolates were determined as A1 mating type, due to formation of a few typical sexual structures when crossed with the A2 mating type of P. cinnamomi and P. cryptogea. Pathogenicity test on non-wounded detached leaves of 19 popular ornamentals, as well as the most frequently imported ones, revealed that 10 host species were susceptible, including Robinia pseudoacacia, which is widely distributed in Serbia. During this study, Cotoneaster horizontalis and C. dammeri were determined to be new experimental hosts of P ramorum. This article provides evidence of P. ramorum introduction into Serbia. Although P. ramorum has not been detected in Serbian production nurseries, its presence outdoors might cause severe damages on susceptible common urban plants in public green and natural ecosystems.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Phytophthora ramorum Occurrence in Ornamentals in Serbia
EP  - 708
IS  - 6
SP  - 703
VL  - 94
DO  - 10.1094/PDIS-94-6-0703
ER  - 

@article{
author = "Bulajić, Aleksandra and Djekić, Ivana and Jović, Jelena and Krnjajić, Slobodan and Vučurović, Ana and Krstić, Branka",
year = "2010",
abstract = "In a survey to determine the presence of Phytophthora ramorum in Serbia, ornamentals from garden centers, nurseries, and private and public gardens, as well as imported plant material, were inspected. In total, 577 plant, soil, and potting media samples were tested using various detection methods: lateral flow diagnostic test, enzyme-linked immunosorbent assay, conventional polymerase chain reaction, and isolation, followed by identification based on growth characteristics in culture and morphological features. P. ramorum was not detected in any of the 162 soil or potting media tested by the baiting method. P. ramorum was detected in 12 Rhododendron samples from one private garden in Zemun (City of Belgrade District) exhibiting symptoms of leaf necrosis and blight and petiole necrosis, and in three samples of Pieris spp. from one garden center exhibiting symptoms of leaf necrosis. Eight Phytophthora isolates were obtained from the positive Rhododendron plants and three isolates from Pieris plants, and all were identified as P. ramorum on the basis of their uniform morphological and growth characteristics. P. ramorum conformation was also made by sequencing of the internal transcribed spacer regions for a single isolate taken from one infected rhododendron and one pieris plant. Serbian isolates were determined as A1 mating type, due to formation of a few typical sexual structures when crossed with the A2 mating type of P. cinnamomi and P. cryptogea. Pathogenicity test on non-wounded detached leaves of 19 popular ornamentals, as well as the most frequently imported ones, revealed that 10 host species were susceptible, including Robinia pseudoacacia, which is widely distributed in Serbia. During this study, Cotoneaster horizontalis and C. dammeri were determined to be new experimental hosts of P ramorum. This article provides evidence of P. ramorum introduction into Serbia. Although P. ramorum has not been detected in Serbian production nurseries, its presence outdoors might cause severe damages on susceptible common urban plants in public green and natural ecosystems.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Phytophthora ramorum Occurrence in Ornamentals in Serbia",
pages = "708-703",
number = "6",
volume = "94",
doi = "10.1094/PDIS-94-6-0703"
}

Bulajić, A., Djekić, I., Jović, J., Krnjajić, S., Vučurović, A.,& Krstić, B.. (2010). Phytophthora ramorum Occurrence in Ornamentals in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 94(6), 703-708.
https://doi.org/10.1094/PDIS-94-6-0703

Bulajić A, Djekić I, Jović J, Krnjajić S, Vučurović A, Krstić B. Phytophthora ramorum Occurrence in Ornamentals in Serbia. in Plant Disease. 2010;94(6):703-708.
doi:10.1094/PDIS-94-6-0703 .

Bulajić, Aleksandra, Djekić, Ivana, Jović, Jelena, Krnjajić, Slobodan, Vučurović, Ana, Krstić, Branka, "Phytophthora ramorum Occurrence in Ornamentals in Serbia" in Plant Disease, 94, no. 6 (2010):703-708,
https://doi.org/10.1094/PDIS-94-6-0703 . .

TY  - JOUR
AU  - Petrović-Obradović, Olivera
AU  - Vukašinović, Dragana
AU  - Vučetić, Andja
AU  - Milovanović, Predrag
AU  - Krnjajić, Slobodan
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1908
AB  - Research has been conducted on apple aphids, throughout many localities in Serbia, during 2007 and 2008. New invasive species, Spiraea aphid, Aphis spiraecola Patch., (synonym Aphis citricola) was found on apple. It was found in about 30 samples. Key for separation of A. spriaecola from very similar A. pomi was given in this paper. .
AB  - U radu se daje prikaz istraživanja biljnih vašiju jabuke, sprovedenih tokom 2007. i 2008. godine, na više lokaliteta u Srbiji. Utvrđeno je prisustvo nove invazivne štetočine jabuke, zelene vaši spiree, Aphis spiraecola Patch., čiji je sinonim A. citricola - zelena vaš citrusa. Pronađena je u oko 30 uzoraka, često u mešanim kolonijama sa zelenom vaši jabuke. U radu se daje ključ pomoću koga se A. spiraecola može razlikovati od veoma slične vrste, A. pomi.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Aphis spiraecola Patch.: New pest of apple in Serbia
T1  - Aphis spiraecola Patch. - nova štetočina jabuke u Srbiji
EP  - 10
IS  - 1
SP  - 7
VL  - 37
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1908
ER  - 

@article{
author = "Petrović-Obradović, Olivera and Vukašinović, Dragana and Vučetić, Andja and Milovanović, Predrag and Krnjajić, Slobodan",
year = "2009",
abstract = "Research has been conducted on apple aphids, throughout many localities in Serbia, during 2007 and 2008. New invasive species, Spiraea aphid, Aphis spiraecola Patch., (synonym Aphis citricola) was found on apple. It was found in about 30 samples. Key for separation of A. spriaecola from very similar A. pomi was given in this paper. ., U radu se daje prikaz istraživanja biljnih vašiju jabuke, sprovedenih tokom 2007. i 2008. godine, na više lokaliteta u Srbiji. Utvrđeno je prisustvo nove invazivne štetočine jabuke, zelene vaši spiree, Aphis spiraecola Patch., čiji je sinonim A. citricola - zelena vaš citrusa. Pronađena je u oko 30 uzoraka, često u mešanim kolonijama sa zelenom vaši jabuke. U radu se daje ključ pomoću koga se A. spiraecola može razlikovati od veoma slične vrste, A. pomi.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Aphis spiraecola Patch.: New pest of apple in Serbia, Aphis spiraecola Patch. - nova štetočina jabuke u Srbiji",
pages = "10-7",
number = "1",
volume = "37",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1908"
}

Petrović-Obradović, O., Vukašinović, D., Vučetić, A., Milovanović, P.,& Krnjajić, S.. (2009). Aphis spiraecola Patch.: New pest of apple in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 37(1), 7-10.
https://hdl.handle.net/21.15107/rcub_agrospace_1908

Petrović-Obradović O, Vukašinović D, Vučetić A, Milovanović P, Krnjajić S. Aphis spiraecola Patch.: New pest of apple in Serbia. in Biljni lekar. 2009;37(1):7-10.
https://hdl.handle.net/21.15107/rcub_agrospace_1908 .

Petrović-Obradović, Olivera, Vukašinović, Dragana, Vučetić, Andja, Milovanović, Predrag, Krnjajić, Slobodan, "Aphis spiraecola Patch.: New pest of apple in Serbia" in Biljni lekar, 37, no. 1 (2009):7-10,
https://hdl.handle.net/21.15107/rcub_agrospace_1908 .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2047
AB  - In a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Incidence and Distribution of Iris yellow spot virus on Onion in Serbia
EP  - 982
IS  - 10
SP  - 976
VL  - 93
DO  - 10.1094/PDIS-93-10-0976
ER  - 

@article{
author = "Bulajić, Aleksandra and Djekić, Ivana and Jović, Jelena and Krnjajić, Slobodan and Vučurović, Ana and Krstić, Branka",
year = "2009",
abstract = "In a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia",
pages = "982-976",
number = "10",
volume = "93",
doi = "10.1094/PDIS-93-10-0976"
}

Bulajić, A., Djekić, I., Jović, J., Krnjajić, S., Vučurović, A.,& Krstić, B.. (2009). Incidence and Distribution of Iris yellow spot virus on Onion in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 93(10), 976-982.
https://doi.org/10.1094/PDIS-93-10-0976

Bulajić A, Djekić I, Jović J, Krnjajić S, Vučurović A, Krstić B. Incidence and Distribution of Iris yellow spot virus on Onion in Serbia. in Plant Disease. 2009;93(10):976-982.
doi:10.1094/PDIS-93-10-0976 .

Bulajić, Aleksandra, Djekić, Ivana, Jović, Jelena, Krnjajić, Slobodan, Vučurović, Ana, Krstić, Branka, "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia" in Plant Disease, 93, no. 10 (2009):976-982,
https://doi.org/10.1094/PDIS-93-10-0976 . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Djekić, Ilija
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2007
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Pathology
T1  - First report of Phytophthora ramorum on Rhododendron sp in Serbia
EP  - 804
IS  - 4
SP  - 804
VL  - 58
DO  - 10.1111/j.1365-3059.2009.02033.x
ER  - 

@article{
author = "Bulajić, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Djekić, Ilija and Krstić, Branka",
year = "2009",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Pathology",
title = "First report of Phytophthora ramorum on Rhododendron sp in Serbia",
pages = "804-804",
number = "4",
volume = "58",
doi = "10.1111/j.1365-3059.2009.02033.x"
}

Bulajić, A., Jović, J., Krnjajić, S., Djekić, I.,& Krstić, B.. (2009). First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology
Wiley-Blackwell, Hoboken., 58(4), 804-804.
https://doi.org/10.1111/j.1365-3059.2009.02033.x

Bulajić A, Jović J, Krnjajić S, Djekić I, Krstić B. First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology. 2009;58(4):804-804.
doi:10.1111/j.1365-3059.2009.02033.x .

Bulajić, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Djekić, Ilija, Krstić, Branka, "First report of Phytophthora ramorum on Rhododendron sp in Serbia" in Plant Pathology, 58, no. 4 (2009):804-804,
https://doi.org/10.1111/j.1365-3059.2009.02033.x . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Petrov, M.
AU  - Djekić, Ilija
AU  - Krstić, Branka
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1814
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia
EP  - 1247
IS  - 8
SP  - 1247
VL  - 92
DO  - 10.1094/PDIS-92-8-1247A
ER  - 

@article{
author = "Bulajić, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Petrov, M. and Djekić, Ilija and Krstić, Branka",
year = "2008",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia",
pages = "1247-1247",
number = "8",
volume = "92",
doi = "10.1094/PDIS-92-8-1247A"
}

Bulajić, A., Jović, J., Krnjajić, S., Petrov, M., Djekić, I.,& Krstić, B.. (2008). First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 92(8), 1247-1247.
https://doi.org/10.1094/PDIS-92-8-1247A

Bulajić A, Jović J, Krnjajić S, Petrov M, Djekić I, Krstić B. First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease. 2008;92(8):1247-1247.
doi:10.1094/PDIS-92-8-1247A .

Bulajić, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Petrov, M., Djekić, Ilija, Krstić, Branka, "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia" in Plant Disease, 92, no. 8 (2008):1247-1247,
https://doi.org/10.1094/PDIS-92-8-1247A . .

TY  - JOUR
AU  - Djekić, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Krstić, Branka
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Berenji, Janoš
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1779
AB  - Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. .
AB  - Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Frequency and molecular detection of cucumber mosaic virus in tobacco crops
T1  - Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana
EP  - 82
IS  - 81
SP  - 70
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1779
ER  - 

@article{
author = "Djekić, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Krstić, Branka and Jović, Jelena and Krnjajić, Slobodan and Berenji, Janoš",
year = "2008",
abstract = "Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. ., Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Frequency and molecular detection of cucumber mosaic virus in tobacco crops, Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana",
pages = "82-70",
number = "81",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1779"
}

Djekić, I., Bulajić, A., Vučurović, A., Krstić, B., Jović, J., Krnjajić, S.,& Berenji, J.. (2008). Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 40(81), 70-82.
https://hdl.handle.net/21.15107/rcub_agrospace_1779

Djekić I, Bulajić A, Vučurović A, Krstić B, Jović J, Krnjajić S, Berenji J. Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje. 2008;40(81):70-82.
https://hdl.handle.net/21.15107/rcub_agrospace_1779 .

Djekić, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Krstić, Branka, Jović, Jelena, Krnjajić, Slobodan, Berenji, Janoš, "Frequency and molecular detection of cucumber mosaic virus in tobacco crops" in Bilten za hmelj, sirak i lekovito bilje, 40, no. 81 (2008):70-82,
https://hdl.handle.net/21.15107/rcub_agrospace_1779 .