TY  - JOUR
AU  - Kostić, Aleksandar Ž
AU  - Milinčić, Danijel D.
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Stanojević, Sladjana P.
AU  - Pešić, Mirjana B.
PY  - 2023
UR  - https://www.mdpi.com/2076-3921/12/7/1424
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6406
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
T2  - Antioxidants
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
SP  - 1424
VL  - 12
DO  - 10.3390/antiox12071424
ER  - 

@article{
author = "Kostić, Aleksandar Ž and Milinčić, Danijel D. and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš M. and Tešić, Živoslav Lj. and Stanojević, Sladjana P. and Pešić, Mirjana B.",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
journal = "Antioxidants, Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
pages = "1424",
volume = "12",
doi = "10.3390/antiox12071424"
}

Kostić, A. Ž., Milinčić, D. D., Špirović Trifunović, B., Nedić, N., Gašić, U. M., Tešić, Ž. Lj., Stanojević, S. P.,& Pešić, M. B.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants, 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić AŽ, Milinčić DD, Špirović Trifunović B, Nedić N, Gašić UM, Tešić ŽL, Stanojević SP, Pešić MB. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar Ž, Milinčić, Danijel D., Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš M., Tešić, Živoslav Lj., Stanojević, Sladjana P., Pešić, Mirjana B., "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Dojčinović, Biljana
AU  - Špirović Trifunović, Bojana
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2022
UR  - https://doi.org/10.1080/03601234.2022.2079348
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6109
AB  - The aim of the current research was to determine the content of (potentially) toxic elements and insecticide residues in monofloral sunflower bee-collected pollen. For micro- and trace elements determination Inductively Coupled Plasma Optical Emission (ICP-OES) analytical method was used while insecticide residue content was monitored by applying Liquid Chromatography-Mass Spectrometry (LC-MS/MS) technique. In total, seventeen micro/trace elements were quantified. None of the twenty four examined insecticides were detected above the limit of detection (LOD) which makes studied sunflower bee-collected pollen eco-friendly both to bees and humans. Based on presence of several toxic as well as potentially toxic elements calculations for estimated weekly intakes (EWI), and oral intakes (OI) were made and used for health risk assessment based on the computation of two different health risk quotients (HQ)- acute (HQA) and long-term (HQL). The obtained results proved that all HQ values for adults were negligible or low except in case of HQL value for arsenic (0.32) which can be characterized as medium. However, in case of children much more precaution is needed due to significant HQL risk for arsenic (1.511). The attained data can help to make additional linkage between bee-collected pollen as food ingredients and potential benefits/risks for human health.
T2  - Journal of Environmental Science and Health, Part B
T2  - Journal of Environmental Science and Health, Part B
T1  - Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment
EP  - 8
IS  - n/a
SP  - 1
VL  - n/a
DO  - 10.1080/03601234.2022.2079348
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Dojčinović, Biljana and Špirović Trifunović, Bojana and Milinčić, Danijel D. and Nedić, Nebojša and Stanojević, Sladjana and Pešić, Mirjana",
year = "2022",
abstract = "The aim of the current research was to determine the content of (potentially) toxic elements and insecticide residues in monofloral sunflower bee-collected pollen. For micro- and trace elements determination Inductively Coupled Plasma Optical Emission (ICP-OES) analytical method was used while insecticide residue content was monitored by applying Liquid Chromatography-Mass Spectrometry (LC-MS/MS) technique. In total, seventeen micro/trace elements were quantified. None of the twenty four examined insecticides were detected above the limit of detection (LOD) which makes studied sunflower bee-collected pollen eco-friendly both to bees and humans. Based on presence of several toxic as well as potentially toxic elements calculations for estimated weekly intakes (EWI), and oral intakes (OI) were made and used for health risk assessment based on the computation of two different health risk quotients (HQ)- acute (HQA) and long-term (HQL). The obtained results proved that all HQ values for adults were negligible or low except in case of HQL value for arsenic (0.32) which can be characterized as medium. However, in case of children much more precaution is needed due to significant HQL risk for arsenic (1.511). The attained data can help to make additional linkage between bee-collected pollen as food ingredients and potential benefits/risks for human health.",
journal = "Journal of Environmental Science and Health, Part B, Journal of Environmental Science and Health, Part B",
title = "Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment",
pages = "8-1",
number = "n/a",
volume = "n/a",
doi = "10.1080/03601234.2022.2079348"
}

Kostić, A. Ž., Dojčinović, B., Špirović Trifunović, B., Milinčić, D. D., Nedić, N., Stanojević, S.,& Pešić, M.. (2022). Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment. in Journal of Environmental Science and Health, Part B, n/a(n/a), 1-8.
https://doi.org/10.1080/03601234.2022.2079348

Kostić AŽ, Dojčinović B, Špirović Trifunović B, Milinčić DD, Nedić N, Stanojević S, Pešić M. Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment. in Journal of Environmental Science and Health, Part B. 2022;n/a(n/a):1-8.
doi:10.1080/03601234.2022.2079348 .

Kostić, Aleksandar Ž., Dojčinović, Biljana, Špirović Trifunović, Bojana, Milinčić, Danijel D., Nedić, Nebojša, Stanojević, Sladjana, Pešić, Mirjana, "Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment" in Journal of Environmental Science and Health, Part B, n/a, no. n/a (2022):1-8,
https://doi.org/10.1080/03601234.2022.2079348 . .

TY  - JOUR
AU  - Popović-Djordjević, Jelena
AU  - Špirović-Trifunović, Bojana
AU  - Pećinar, Ilinka
AU  - Fernando Cappa de Oliveira, Luiz
AU  - Krstić, Đurđa
AU  - Mihajlović, Dragana
AU  - Akšić, Milica Fotirić
AU  - Simal-Gandara, Jesus
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6235
AB  - Rosehip (Rosa canina L.) seeds are rich in bioactive compounds and nutrients and hence with a great potential to be employed in production of functional foods. This work aimed to evaluate the fatty acid composition of seed oil from wild and cultivated rosehip collected at different locations in the Republic of Serbia. Unsaturated fatty acids were dominant in majority of seed oil samples, with linoleic (LA), α-linolenic (ALA) and oleic (OA) acids (24.53–46.68 %, 4.73–12.39 % and 3.89–13.82 %, respectively) as the most abundant ones. Based on the analyses of most dominant bands in Raman spectra of seeds (∼1265 and ∼1660 cm-1) characteristic for unsaturated fatty acids, ANOVA revealed significantly higher content in two seed samples (5SW and 10SC). Ratios of UFAs/ SFAs, ω-6/ω-3 and LA/ALA and desirable fatty acids (DFA) indicated that most studied rosehip seed oils showed good quality. Factors such as genetic characteristics and agro-ecological conditions most likely affected FAs composition of seed oils. © 2022 The Authors
T2  - Industrial Crops and Products
T2  - Industrial Crops and Products
T1  - Fatty acids in seed oil of wild and cultivated rosehip (Rosa canina L.) from different locations in Serbia
VL  - 191
DO  - 10.1016/j.indcrop.2022.115797
ER  - 

@article{
author = "Popović-Djordjević, Jelena and Špirović-Trifunović, Bojana and Pećinar, Ilinka and Fernando Cappa de Oliveira, Luiz and Krstić, Đurđa and Mihajlović, Dragana and Akšić, Milica Fotirić and Simal-Gandara, Jesus",
year = "2022",
abstract = "Rosehip (Rosa canina L.) seeds are rich in bioactive compounds and nutrients and hence with a great potential to be employed in production of functional foods. This work aimed to evaluate the fatty acid composition of seed oil from wild and cultivated rosehip collected at different locations in the Republic of Serbia. Unsaturated fatty acids were dominant in majority of seed oil samples, with linoleic (LA), α-linolenic (ALA) and oleic (OA) acids (24.53–46.68 %, 4.73–12.39 % and 3.89–13.82 %, respectively) as the most abundant ones. Based on the analyses of most dominant bands in Raman spectra of seeds (∼1265 and ∼1660 cm-1) characteristic for unsaturated fatty acids, ANOVA revealed significantly higher content in two seed samples (5SW and 10SC). Ratios of UFAs/ SFAs, ω-6/ω-3 and LA/ALA and desirable fatty acids (DFA) indicated that most studied rosehip seed oils showed good quality. Factors such as genetic characteristics and agro-ecological conditions most likely affected FAs composition of seed oils. © 2022 The Authors",
journal = "Industrial Crops and Products, Industrial Crops and Products",
title = "Fatty acids in seed oil of wild and cultivated rosehip (Rosa canina L.) from different locations in Serbia",
volume = "191",
doi = "10.1016/j.indcrop.2022.115797"
}

Popović-Djordjević, J., Špirović-Trifunović, B., Pećinar, I., Fernando Cappa de Oliveira, L., Krstić, Đ., Mihajlović, D., Akšić, M. F.,& Simal-Gandara, J.. (2022). Fatty acids in seed oil of wild and cultivated rosehip (Rosa canina L.) from different locations in Serbia. in Industrial Crops and Products, 191.
https://doi.org/10.1016/j.indcrop.2022.115797

Popović-Djordjević J, Špirović-Trifunović B, Pećinar I, Fernando Cappa de Oliveira L, Krstić Đ, Mihajlović D, Akšić MF, Simal-Gandara J. Fatty acids in seed oil of wild and cultivated rosehip (Rosa canina L.) from different locations in Serbia. in Industrial Crops and Products. 2022;191.
doi:10.1016/j.indcrop.2022.115797 .

Popović-Djordjević, Jelena, Špirović-Trifunović, Bojana, Pećinar, Ilinka, Fernando Cappa de Oliveira, Luiz, Krstić, Đurđa, Mihajlović, Dragana, Akšić, Milica Fotirić, Simal-Gandara, Jesus, "Fatty acids in seed oil of wild and cultivated rosehip (Rosa canina L.) from different locations in Serbia" in Industrial Crops and Products, 191 (2022),
https://doi.org/10.1016/j.indcrop.2022.115797 . .

TY  - JOUR
AU  - Barać, Miroljub
AU  - Vučić, Tanja
AU  - Špirović-Trifunović, Bojana
AU  - Barać, Nevena
AU  - Smiljanić, Milenko
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6165
AB  - Kajmak is a specific dairy product with long term tradition of manufacture in Serbia and few South-East European countries. It is manufactured from the thin layer formed on the surface of hot milk during long cooling process. It may be consumed as a fresh, immediately after manufacture, or after a maturation period, as a ripened Kajmak. Kajmak is ripened usually up to 30 days at 15-180C or stored in refrigerator at 40C. In this work we investigated the effects of ripening of Kajmak in refrigerator at 40C and at 160C up to 28 days on protein profiles, fatty acid profiles and health fatty indices. Ripening temperature significantly affects protein and fatty acid profiles of Kajmak. Ripening at low temperature induces slow proteolysis, the change of fatty acid profile and health fatty acid indices. At low temperature the most susceptible to proteolysis was β-casein. Due to similar fatty acid profiles, these samples were characterized with high health fatty acid indices such as atherogenicity index (3.81-4.03) and the thrombogenicity index (4.62-4.95). Ripening at 160C induces complete hydrolysis of κ-CN, significant decrease of αs-and β-CN content and improvement of health fatty indices. © 2022, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.
T2  - Food Science and Technology (Brazil)
T2  - Food Science and Technology (Brazil)
T1  - Protein and fatty acid profiles of Kajmak ripened at two different temperatures
VL  - 42
DO  - 10.1590/fst.63322
ER  - 

@article{
author = "Barać, Miroljub and Vučić, Tanja and Špirović-Trifunović, Bojana and Barać, Nevena and Smiljanić, Milenko",
year = "2022",
abstract = "Kajmak is a specific dairy product with long term tradition of manufacture in Serbia and few South-East European countries. It is manufactured from the thin layer formed on the surface of hot milk during long cooling process. It may be consumed as a fresh, immediately after manufacture, or after a maturation period, as a ripened Kajmak. Kajmak is ripened usually up to 30 days at 15-180C or stored in refrigerator at 40C. In this work we investigated the effects of ripening of Kajmak in refrigerator at 40C and at 160C up to 28 days on protein profiles, fatty acid profiles and health fatty indices. Ripening temperature significantly affects protein and fatty acid profiles of Kajmak. Ripening at low temperature induces slow proteolysis, the change of fatty acid profile and health fatty acid indices. At low temperature the most susceptible to proteolysis was β-casein. Due to similar fatty acid profiles, these samples were characterized with high health fatty acid indices such as atherogenicity index (3.81-4.03) and the thrombogenicity index (4.62-4.95). Ripening at 160C induces complete hydrolysis of κ-CN, significant decrease of αs-and β-CN content and improvement of health fatty indices. © 2022, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.",
journal = "Food Science and Technology (Brazil), Food Science and Technology (Brazil)",
title = "Protein and fatty acid profiles of Kajmak ripened at two different temperatures",
volume = "42",
doi = "10.1590/fst.63322"
}

Barać, M., Vučić, T., Špirović-Trifunović, B., Barać, N.,& Smiljanić, M.. (2022). Protein and fatty acid profiles of Kajmak ripened at two different temperatures. in Food Science and Technology (Brazil), 42.
https://doi.org/10.1590/fst.63322

Barać M, Vučić T, Špirović-Trifunović B, Barać N, Smiljanić M. Protein and fatty acid profiles of Kajmak ripened at two different temperatures. in Food Science and Technology (Brazil). 2022;42.
doi:10.1590/fst.63322 .

Barać, Miroljub, Vučić, Tanja, Špirović-Trifunović, Bojana, Barać, Nevena, Smiljanić, Milenko, "Protein and fatty acid profiles of Kajmak ripened at two different temperatures" in Food Science and Technology (Brazil), 42 (2022),
https://doi.org/10.1590/fst.63322 . .

TY  - JOUR
AU  - Udovički, Božidar
AU  - Stanković, Slavica
AU  - Tomić, Nikola
AU  - Đekić, Ilija
AU  - Šmigić, Nada
AU  - Špirović Trifunović, Bojana
AU  - Milićević, Dragan
AU  - Rajković, Andreja
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5975
AB  - This study investigated the effectiveness of the ultraviolet irradiation of the C region (UV–C) for the decontamination of Aspergillus flavus and aflatoxin B1 (AFB1) on artificially inoculated maize and peanut using innovative decontamination equipment that supports vibrations to achieve semi-fluidization of the grain/kernel material and getting equal irradiation of all surfaces of irradiated foods. UV irradiation is a non-thermal alternative to thermal processes commonly used in food processing with a well-known effect on AFB1 degradation. Samples of maize and peanut were exposed to UV-C irradiation with a total dose in the range of 1080–8370 mJ/cm2. Analysis by Tracker and ImageJ software confirmed the even distribution of irradiation on all surfaces during the entire duration of exposure. The highest reduction of A. flavus count was observed after ten days of incubation and irradiation treatment delivering a dose of 8370 mJ/cm2 achieving A. flavus count reduction of 4.4 log CFU/g in maize and 3.1 log CFU/g in peanut. Depending on the treatment, AFB1 reduction level in maize ranged from 17 to 43% and in peanut ranged from 14 to 51%. Sensory and physical testing of the peanut samples showed only minimal changes in the evaluated characteristics caused by different levels of the UV-C treatment. Presented results demonstrate a potential for the use of the presented approach as an effective reduction strategy for both A. flavus and AFB1 in maize and peanut.
PB  - Elsevier Ltd
T2  - Food Control
T1  - Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment
VL  - 134
DO  - 10.1016/j.foodcont.2021.108691
ER  - 

@article{
author = "Udovički, Božidar and Stanković, Slavica and Tomić, Nikola and Đekić, Ilija and Šmigić, Nada and Špirović Trifunović, Bojana and Milićević, Dragan and Rajković, Andreja",
year = "2022",
abstract = "This study investigated the effectiveness of the ultraviolet irradiation of the C region (UV–C) for the decontamination of Aspergillus flavus and aflatoxin B1 (AFB1) on artificially inoculated maize and peanut using innovative decontamination equipment that supports vibrations to achieve semi-fluidization of the grain/kernel material and getting equal irradiation of all surfaces of irradiated foods. UV irradiation is a non-thermal alternative to thermal processes commonly used in food processing with a well-known effect on AFB1 degradation. Samples of maize and peanut were exposed to UV-C irradiation with a total dose in the range of 1080–8370 mJ/cm2. Analysis by Tracker and ImageJ software confirmed the even distribution of irradiation on all surfaces during the entire duration of exposure. The highest reduction of A. flavus count was observed after ten days of incubation and irradiation treatment delivering a dose of 8370 mJ/cm2 achieving A. flavus count reduction of 4.4 log CFU/g in maize and 3.1 log CFU/g in peanut. Depending on the treatment, AFB1 reduction level in maize ranged from 17 to 43% and in peanut ranged from 14 to 51%. Sensory and physical testing of the peanut samples showed only minimal changes in the evaluated characteristics caused by different levels of the UV-C treatment. Presented results demonstrate a potential for the use of the presented approach as an effective reduction strategy for both A. flavus and AFB1 in maize and peanut.",
publisher = "Elsevier Ltd",
journal = "Food Control",
title = "Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment",
volume = "134",
doi = "10.1016/j.foodcont.2021.108691"
}

Udovički, B., Stanković, S., Tomić, N., Đekić, I., Šmigić, N., Špirović Trifunović, B., Milićević, D.,& Rajković, A.. (2022). Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment. in Food Control
Elsevier Ltd., 134.
https://doi.org/10.1016/j.foodcont.2021.108691

Udovički B, Stanković S, Tomić N, Đekić I, Šmigić N, Špirović Trifunović B, Milićević D, Rajković A. Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment. in Food Control. 2022;134.
doi:10.1016/j.foodcont.2021.108691 .

Udovički, Božidar, Stanković, Slavica, Tomić, Nikola, Đekić, Ilija, Šmigić, Nada, Špirović Trifunović, Bojana, Milićević, Dragan, Rajković, Andreja, "Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment" in Food Control, 134 (2022),
https://doi.org/10.1016/j.foodcont.2021.108691 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5886
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - MDPI AG
T2  - Antioxidants
T1  - Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen
IS  - 7
SP  - 1091
VL  - 10
DO  - 10.3390/antiox10071091
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen",
number = "7",
pages = "1091",
volume = "10",
doi = "10.3390/antiox10071091"
}

Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants
MDPI AG., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - JOUR
AU  - Šćepanović, Maja
AU  - Sarić-Krsmanović, Marija
AU  - Šoštarčić, Valentina
AU  - Brijačak, Ema
AU  - Lakić, Josip
AU  - Špirović Trifunović, Bojana
AU  - Gajić Umiljendić, Jelena
AU  - Radivojević, Ljiljana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5839
AB  - Several cover crops (CCs) exert allelopathic effects that suppress weed growth. The aim of the present study was to evaluate the effects of aqueous extracts containing different concentrations [0, 0.5, 1, 2.5, 5, 7.5 and 10% (w/v)] of Brassicaceae CCs (Sinapis alba, Raphanus sativus, Camellina sativa) and of the CCs Fagopyrum esculentum and Guizotia abyssinica on germination and early growth of Ambrosia artemisiifolia L. The allelopathic effects were species and concentration-dependent. C. sativa, for example, caused the greatest potential to inhibit germination, shoot, radicle length and fresh seedling weight, whereas S. alba and R. sativus inhibited germination and early growth of A. artemisiifolia only at concentrations ≥ 7.5%. In contrast, no inhibition was observed when aqueous extracts of F. escultneum and G. abyssinica were added at any of tested concentration. Liquid chro-matography-tandem mass spectrometry detected 15 phenolic compounds in Brassicaceae CCs with the highest content (µg/g) of vanillin (48.8), chlorogenic acid (1057), vanilic acid (79), caffeic acid (102.5) and syringic acid (27.3) in C. sativa. Our results suggest that C. sativa is the most allelopathic CCs and that the fruits of C. sativa are the plant organs richest in allelochemicals.
PB  - MDPI AG
T2  - Plants
T1  - Inhibitory Effects of Brassicaceae Cover Crop on Ambrosia artemisiifolia Germination and Early Growth
IS  - 4
SP  - 794
VL  - 10
DO  - 10.3390/plants10040794
ER  - 

@article{
author = "Šćepanović, Maja and Sarić-Krsmanović, Marija and Šoštarčić, Valentina and Brijačak, Ema and Lakić, Josip and Špirović Trifunović, Bojana and Gajić Umiljendić, Jelena and Radivojević, Ljiljana",
year = "2021",
abstract = "Several cover crops (CCs) exert allelopathic effects that suppress weed growth. The aim of the present study was to evaluate the effects of aqueous extracts containing different concentrations [0, 0.5, 1, 2.5, 5, 7.5 and 10% (w/v)] of Brassicaceae CCs (Sinapis alba, Raphanus sativus, Camellina sativa) and of the CCs Fagopyrum esculentum and Guizotia abyssinica on germination and early growth of Ambrosia artemisiifolia L. The allelopathic effects were species and concentration-dependent. C. sativa, for example, caused the greatest potential to inhibit germination, shoot, radicle length and fresh seedling weight, whereas S. alba and R. sativus inhibited germination and early growth of A. artemisiifolia only at concentrations ≥ 7.5%. In contrast, no inhibition was observed when aqueous extracts of F. escultneum and G. abyssinica were added at any of tested concentration. Liquid chro-matography-tandem mass spectrometry detected 15 phenolic compounds in Brassicaceae CCs with the highest content (µg/g) of vanillin (48.8), chlorogenic acid (1057), vanilic acid (79), caffeic acid (102.5) and syringic acid (27.3) in C. sativa. Our results suggest that C. sativa is the most allelopathic CCs and that the fruits of C. sativa are the plant organs richest in allelochemicals.",
publisher = "MDPI AG",
journal = "Plants",
title = "Inhibitory Effects of Brassicaceae Cover Crop on Ambrosia artemisiifolia Germination and Early Growth",
number = "4",
pages = "794",
volume = "10",
doi = "10.3390/plants10040794"
}

Šćepanović, M., Sarić-Krsmanović, M., Šoštarčić, V., Brijačak, E., Lakić, J., Špirović Trifunović, B., Gajić Umiljendić, J.,& Radivojević, L.. (2021). Inhibitory Effects of Brassicaceae Cover Crop on Ambrosia artemisiifolia Germination and Early Growth. in Plants
MDPI AG., 10(4), 794.
https://doi.org/10.3390/plants10040794

Šćepanović M, Sarić-Krsmanović M, Šoštarčić V, Brijačak E, Lakić J, Špirović Trifunović B, Gajić Umiljendić J, Radivojević L. Inhibitory Effects of Brassicaceae Cover Crop on Ambrosia artemisiifolia Germination and Early Growth. in Plants. 2021;10(4):794.
doi:10.3390/plants10040794 .

Šćepanović, Maja, Sarić-Krsmanović, Marija, Šoštarčić, Valentina, Brijačak, Ema, Lakić, Josip, Špirović Trifunović, Bojana, Gajić Umiljendić, Jelena, Radivojević, Ljiljana, "Inhibitory Effects of Brassicaceae Cover Crop on Ambrosia artemisiifolia Germination and Early Growth" in Plants, 10, no. 4 (2021):794,
https://doi.org/10.3390/plants10040794 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Špirović Trifunović, Bojana D.
AU  - Stanojević, Sladjana P.
AU  - Lević, Steva
AU  - Nedić, Nebojša
AU  - Nedović, Viktor
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5823
AB  - The aim of this study was to examine nutritional (total lipid, carbohydrate, protein and ash content, fatty acids and protein profile) and techno-functional properties of monofloral bee-collected sunflower pollen (Helianthus annuus L.). The content of water, total protein, carbohydrate, lipid and ash was 24.99, 14.36, 82.01, 1.62 and 2.01 g/100g DW, respectively, with the total energy value of 400.06 kcal/100 g DW. The fatty acid profile revealed the presence of five fatty acids with the stearic acid as dominant one (31.4%) followed by α-linoleic (20.7%), pentadecanoic (18.2%), heneicosanoic (17.1%) and palmitic (12.5%) acids. FTIR analysis of bee pollen grains confirmed the presence of the main pollen chemical constituents such as proteins, water, carbohydrates and lipids, but also the presence of sporopollenin and polyphenols. Raman spectroscopy analysis indicated that the surface of pollen grains was rich in carotenoids. Low protein (3.64 g/100g DW) but high carbohydrate (77.09 g/100 g DW) solubility of bee pollen was observed. The good emulsifying properties (ESI, 19.98 min; EAI, 80.54 m2/g) and excellent oil (2.43 g/g DW) but poor water (0.87 g/g DW) absorption capacities were also registered. On the other hand, the sunflower bee pollen did not show foaming properties. These findings indicate to its applicability as useful nutritional, lipophilic and anti-foaming food ingredients.
PB  - United Arab Emirates University
T2  - Emirates Journal of Food and Agriculture
T1  - Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen
EP  - 777
IS  - 11
SP  - 768
VL  - 32
DO  - 10.9755/ejfa.2020.v32.i11.2188
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Špirović Trifunović, Bojana D. and Stanojević, Sladjana P. and Lević, Steva and Nedić, Nebojša and Nedović, Viktor and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2020",
abstract = "The aim of this study was to examine nutritional (total lipid, carbohydrate, protein and ash content, fatty acids and protein profile) and techno-functional properties of monofloral bee-collected sunflower pollen (Helianthus annuus L.). The content of water, total protein, carbohydrate, lipid and ash was 24.99, 14.36, 82.01, 1.62 and 2.01 g/100g DW, respectively, with the total energy value of 400.06 kcal/100 g DW. The fatty acid profile revealed the presence of five fatty acids with the stearic acid as dominant one (31.4%) followed by α-linoleic (20.7%), pentadecanoic (18.2%), heneicosanoic (17.1%) and palmitic (12.5%) acids. FTIR analysis of bee pollen grains confirmed the presence of the main pollen chemical constituents such as proteins, water, carbohydrates and lipids, but also the presence of sporopollenin and polyphenols. Raman spectroscopy analysis indicated that the surface of pollen grains was rich in carotenoids. Low protein (3.64 g/100g DW) but high carbohydrate (77.09 g/100 g DW) solubility of bee pollen was observed. The good emulsifying properties (ESI, 19.98 min; EAI, 80.54 m2/g) and excellent oil (2.43 g/g DW) but poor water (0.87 g/g DW) absorption capacities were also registered. On the other hand, the sunflower bee pollen did not show foaming properties. These findings indicate to its applicability as useful nutritional, lipophilic and anti-foaming food ingredients.",
publisher = "United Arab Emirates University",
journal = "Emirates Journal of Food and Agriculture",
title = "Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen",
pages = "777-768",
number = "11",
volume = "32",
doi = "10.9755/ejfa.2020.v32.i11.2188"
}

Kostić, A. Ž., Milinčić, D. D., Špirović Trifunović, B. D., Stanojević, S. P., Lević, S., Nedić, N., Nedović, V., Tešić, Ž. Lj.,& Pešić, M. B.. (2020). Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen. in Emirates Journal of Food and Agriculture
United Arab Emirates University., 32(11), 768-777.
https://doi.org/10.9755/ejfa.2020.v32.i11.2188

Kostić AŽ, Milinčić DD, Špirović Trifunović BD, Stanojević SP, Lević S, Nedić N, Nedović V, Tešić ŽL, Pešić MB. Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen. in Emirates Journal of Food and Agriculture. 2020;32(11):768-777.
doi:10.9755/ejfa.2020.v32.i11.2188 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Špirović Trifunović, Bojana D., Stanojević, Sladjana P., Lević, Steva, Nedić, Nebojša, Nedović, Viktor, Tešić, Živoslav Lj., Pešić, Mirjana B., "Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen" in Emirates Journal of Food and Agriculture, 32, no. 11 (2020):768-777,
https://doi.org/10.9755/ejfa.2020.v32.i11.2188 . .

TY  - JOUR
AU  - Vasić, Dušan
AU  - Paunović, Dragana
AU  - Špirović-Trifunović, Bojana
AU  - Miladinović, Jelena
AU  - Vujošević, Lazar
AU  - Djinović, Dušica
AU  - Popović-Djordjević, Jelena
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5452
AB  - Rosehip is a pseudo-fruit of the rose plant, one of the most widespread wild species of the Rosa genus in Serbia. Due to its nutritional and sensory properties, rosehip is widely utilized for the production of jams, marmalades, juices, teas, etc. On the other hand, rosehip seeds are waste material from the food industry, which represent are rich source of fatty acids. The aim of this study was to assess the fatty acids profile of rosehip seeds by applying two extraction methods: ultrasound-assisted extraction combined with organic solvent extraction (UAE/OSCE) and organic solvent conventional extraction (OSCE). The identification and quantification of fatty acids (FA), in the form of methyl esters, were performed by gas chromatography with a flame ionization detector (GC/FID). Based on the obtained results, it was observed that the composition of fatty acids and their relative amounts were influenced by the applied method, as well as by the solvent-to-sample ratio. The analysis revealed the presence of unsaturated fatty acids (UFA) as the dominant ones in most studied samples, whereas the most abundant fatty acids were, in descending order, stearic acid (48.11%), linoleic acid (35.38%), palmitoleic acid (33.78%) and eicosadienoic acid (30.57%).
AB  - Šipurak je pseudo-plod biljke ruže, jedne od najrasprostranjenijih divljih vrsta iz roda Rosa u Srbiji. Zahvaljujući nutritivnim i senzornim svojstvima, šipurak ima široku primenu u proizvodnji džemova, marmelada, sokova, čajeva itd. Semenke šipurka su otpadni materijal u prehrambenoj industriji koji predstavlja dobar izvor masnih kiselina. Cilj ovog rada bio je da se proceni profil masnih kiselina u semenkama šipurka primenom dve metode ekstrakcije: ultrazvučne ekstrakcije u kombinaciji sa ekstrakcijom organskim rastvaračem (UZE/KEOR) i konvencionalne ekstrakcije organskim rastvaračem (KEOR). Identifikacija masnih kiselina (MK), u formi metil estara, izvršena je metodom gasne hromatografije sa plameno-jonizujućim detektorom (GC/FID). Na osnovu dobijenih rezultata, uočeno je da su primenjene metode ekstrakcije, kao i odnos rastvarača i mase uzorka, imali uticaj na sastav masnih kiselina i njihovu relativnu obilnost. Nezasićene masne kiseline (NMK) identifikovane su kao dominantne u većini ispitivanih uzoraka. Najzastupljenije masne kiseline su bile stearinska (48,11%), linolna (35,38%), palmitoleinska (33,78%) i eikosadienoinska kiselina (30,57%).
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Fatty acid composition of rosehip seed oil
EP  - 49
IS  - 49
SP  - 45
VL  - 25
DO  - 10.5937/AASer2049045V
ER  - 

@article{
author = "Vasić, Dušan and Paunović, Dragana and Špirović-Trifunović, Bojana and Miladinović, Jelena and Vujošević, Lazar and Djinović, Dušica and Popović-Djordjević, Jelena",
year = "2020",
abstract = "Rosehip is a pseudo-fruit of the rose plant, one of the most widespread wild species of the Rosa genus in Serbia. Due to its nutritional and sensory properties, rosehip is widely utilized for the production of jams, marmalades, juices, teas, etc. On the other hand, rosehip seeds are waste material from the food industry, which represent are rich source of fatty acids. The aim of this study was to assess the fatty acids profile of rosehip seeds by applying two extraction methods: ultrasound-assisted extraction combined with organic solvent extraction (UAE/OSCE) and organic solvent conventional extraction (OSCE). The identification and quantification of fatty acids (FA), in the form of methyl esters, were performed by gas chromatography with a flame ionization detector (GC/FID). Based on the obtained results, it was observed that the composition of fatty acids and their relative amounts were influenced by the applied method, as well as by the solvent-to-sample ratio. The analysis revealed the presence of unsaturated fatty acids (UFA) as the dominant ones in most studied samples, whereas the most abundant fatty acids were, in descending order, stearic acid (48.11%), linoleic acid (35.38%), palmitoleic acid (33.78%) and eicosadienoic acid (30.57%)., Šipurak je pseudo-plod biljke ruže, jedne od najrasprostranjenijih divljih vrsta iz roda Rosa u Srbiji. Zahvaljujući nutritivnim i senzornim svojstvima, šipurak ima široku primenu u proizvodnji džemova, marmelada, sokova, čajeva itd. Semenke šipurka su otpadni materijal u prehrambenoj industriji koji predstavlja dobar izvor masnih kiselina. Cilj ovog rada bio je da se proceni profil masnih kiselina u semenkama šipurka primenom dve metode ekstrakcije: ultrazvučne ekstrakcije u kombinaciji sa ekstrakcijom organskim rastvaračem (UZE/KEOR) i konvencionalne ekstrakcije organskim rastvaračem (KEOR). Identifikacija masnih kiselina (MK), u formi metil estara, izvršena je metodom gasne hromatografije sa plameno-jonizujućim detektorom (GC/FID). Na osnovu dobijenih rezultata, uočeno je da su primenjene metode ekstrakcije, kao i odnos rastvarača i mase uzorka, imali uticaj na sastav masnih kiselina i njihovu relativnu obilnost. Nezasićene masne kiseline (NMK) identifikovane su kao dominantne u većini ispitivanih uzoraka. Najzastupljenije masne kiseline su bile stearinska (48,11%), linolna (35,38%), palmitoleinska (33,78%) i eikosadienoinska kiselina (30,57%).",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Fatty acid composition of rosehip seed oil",
pages = "49-45",
number = "49",
volume = "25",
doi = "10.5937/AASer2049045V"
}

Vasić, D., Paunović, D., Špirović-Trifunović, B., Miladinović, J., Vujošević, L., Djinović, D.,& Popović-Djordjević, J.. (2020). Fatty acid composition of rosehip seed oil. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 25(49), 45-49.
https://doi.org/10.5937/AASer2049045V

Vasić D, Paunović D, Špirović-Trifunović B, Miladinović J, Vujošević L, Djinović D, Popović-Djordjević J. Fatty acid composition of rosehip seed oil. in Acta agriculturae Serbica. 2020;25(49):45-49.
doi:10.5937/AASer2049045V .

Vasić, Dušan, Paunović, Dragana, Špirović-Trifunović, Bojana, Miladinović, Jelena, Vujošević, Lazar, Djinović, Dušica, Popović-Djordjević, Jelena, "Fatty acid composition of rosehip seed oil" in Acta agriculturae Serbica, 25, no. 49 (2020):45-49,
https://doi.org/10.5937/AASer2049045V . .

TY  - JOUR
AU  - Milincić, Danijel D.
AU  - Vojinović, Uroš
AU  - Kostić, Aleksandar
AU  - Pešić, Mirjana
AU  - Špirović-Trifunović, Bojana
AU  - Brkić, Dragica
AU  - Stević, Milan
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja S.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5371
AB  - The aim of this study was to investigate the bioaccessibility of pesticide residues in blueberries (com-mercial and sample from controlled field trial) from Serbia, involving the presence of a complex food matrix and to assess the potential risk to human health. The presence of nine active substances (azox-ystrobin, boscalid, fludioxonil, cyprodinil, pyrimethanil, pyridaben, pyriproxyfen, acetamiprid and thia-metoxam) in initial blueberry samples was determined in concentration range from 5.15 mu g/kg for thiametoxam to 187 mu g/kg for azoxystrobin. Clothianidin, metabolite of thiametoxam, was not detected in any blueberry sample. However, after in vitro digestion, the content of initially detected pesticides residues was significantly decreased or it was below limit of quantification resulting in the total bio-accessibility of about 15%. Azoxystrobin, pyrimethanil and fludioxonil was quantified in digestive juice at concentrations which were about 81%, 37% and 10% less than the inital concentration, respectively. The presence of food matrix during digestion of blueberries even more severely reduced concentration of pesticide residues (total bioaccessibility was about 7%) compared to digestion without the food matrix. Only azoxystrobin was quantified after digestion with food matrix in concentration of 27 mu g/kg in sample from controlled field trial and detected in two commercial samples but below the limit of quantification. Furthermore, chronic risk assessment indicated that risk is acceptable for the health of different human subpopulation groups. The current study on pesticides residues, most commonly applied on blueberries, provides for the first time an insight into their bioaccessibility under conditions that mimic physiological environment of human digestive tract.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix
VL  - 252
DO  - 10.1016/j.chemosphere.2020.126568
ER  - 

@article{
author = "Milincić, Danijel D. and Vojinović, Uroš and Kostić, Aleksandar and Pešić, Mirjana and Špirović-Trifunović, Bojana and Brkić, Dragica and Stević, Milan and Kojić, Milan and Stanisavljević, Nemanja S.",
year = "2020",
abstract = "The aim of this study was to investigate the bioaccessibility of pesticide residues in blueberries (com-mercial and sample from controlled field trial) from Serbia, involving the presence of a complex food matrix and to assess the potential risk to human health. The presence of nine active substances (azox-ystrobin, boscalid, fludioxonil, cyprodinil, pyrimethanil, pyridaben, pyriproxyfen, acetamiprid and thia-metoxam) in initial blueberry samples was determined in concentration range from 5.15 mu g/kg for thiametoxam to 187 mu g/kg for azoxystrobin. Clothianidin, metabolite of thiametoxam, was not detected in any blueberry sample. However, after in vitro digestion, the content of initially detected pesticides residues was significantly decreased or it was below limit of quantification resulting in the total bio-accessibility of about 15%. Azoxystrobin, pyrimethanil and fludioxonil was quantified in digestive juice at concentrations which were about 81%, 37% and 10% less than the inital concentration, respectively. The presence of food matrix during digestion of blueberries even more severely reduced concentration of pesticide residues (total bioaccessibility was about 7%) compared to digestion without the food matrix. Only azoxystrobin was quantified after digestion with food matrix in concentration of 27 mu g/kg in sample from controlled field trial and detected in two commercial samples but below the limit of quantification. Furthermore, chronic risk assessment indicated that risk is acceptable for the health of different human subpopulation groups. The current study on pesticides residues, most commonly applied on blueberries, provides for the first time an insight into their bioaccessibility under conditions that mimic physiological environment of human digestive tract.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix",
volume = "252",
doi = "10.1016/j.chemosphere.2020.126568"
}

Milincić, D. D., Vojinović, U., Kostić, A., Pešić, M., Špirović-Trifunović, B., Brkić, D., Stević, M., Kojić, M.,& Stanisavljević, N. S.. (2020). In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 252.
https://doi.org/10.1016/j.chemosphere.2020.126568

Milincić DD, Vojinović U, Kostić A, Pešić M, Špirović-Trifunović B, Brkić D, Stević M, Kojić M, Stanisavljević NS. In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix. in Chemosphere. 2020;252.
doi:10.1016/j.chemosphere.2020.126568 .

Milincić, Danijel D., Vojinović, Uroš, Kostić, Aleksandar, Pešić, Mirjana, Špirović-Trifunović, Bojana, Brkić, Dragica, Stević, Milan, Kojić, Milan, Stanisavljević, Nemanja S., "In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix" in Chemosphere, 252 (2020),
https://doi.org/10.1016/j.chemosphere.2020.126568 . .

TY  - JOUR
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar
AU  - Špirović-Trifunović, Bojana
AU  - Tešić, Živoslav
AU  - Tosti, Tomislav B.
AU  - Dramicanin, Aleksandra M.
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5392
AB  - The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly w-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value
EP  - 319
IS  - 3
SP  - 305
VL  - 85
DO  - 10.2298/JSC190713117M
ER  - 

@article{
author = "Milincić, Danijel D. and Kostić, Aleksandar and Špirović-Trifunović, Bojana and Tešić, Živoslav and Tosti, Tomislav B. and Dramicanin, Aleksandra M. and Barać, Miroljub and Pešić, Mirjana",
year = "2020",
abstract = "The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly w-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value",
pages = "319-305",
number = "3",
volume = "85",
doi = "10.2298/JSC190713117M"
}

Milincić, D. D., Kostić, A., Špirović-Trifunović, B., Tešić, Ž., Tosti, T. B., Dramicanin, A. M., Barać, M.,& Pešić, M.. (2020). Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 85(3), 305-319.
https://doi.org/10.2298/JSC190713117M

Milincić DD, Kostić A, Špirović-Trifunović B, Tešić Ž, Tosti TB, Dramicanin AM, Barać M, Pešić M. Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2020;85(3):305-319.
doi:10.2298/JSC190713117M .

Milincić, Danijel D., Kostić, Aleksandar, Špirović-Trifunović, Bojana, Tešić, Živoslav, Tosti, Tomislav B., Dramicanin, Aleksandra M., Barać, Miroljub, Pešić, Mirjana, "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 85, no. 3 (2020):305-319,
https://doi.org/10.2298/JSC190713117M . .

TY  - THES
AU  - Špirović-Trifunović, Bojana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/61
AB  - A fast, selective and sensitive reversed-phased liquid chromatography with tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous quantification of 41 pesticide residues and ochratoxin A (ОТА) in grapes and wine. For increasing sensitivity of mass spectrometer multiple reaction monitoring (MRM) was transferred into time segmented method, dynamic multiple reaction monitoring (dMRM). Grape and wine extraction and purification for both, pesticide residues and OTA, were performed with a modified Quick Easy Cheap Effective Rugged and Safe (QuEChERS) method. For OTA LC-MS/MS analysis acetonitrile extract was directly injected into system, and for pesticide analysis extracts were purified with dispersive solid phase extraction for pigmented samples (black grapes and red wine) and for fruits and vegetables (white grapes and wine). MS/MS conditions were optimized in order to increase selectivity, selecting the corresponding product ions (precursor-to-fragment m/z 404 → 239 and m/z 404 → m/z 221) for quantification and identification. For pesticide residues, MS/MS aquisition parameters, SRM transitions, fragment and colision energies, from database were used. For method validation, the performance of the method was assessed and compared with the European Commission (EC) Regulations 401/2006 for OTA and with the SANTE/11813/2017 document for pesticide residues, by studying the linearity, limit of detection (LOD), limit of quantification (LOQ), matrix effect, accuracy and precision. Linearity for quantification pesticide residues in grapes and wine was performed in mobile phase and in sample matrixes (0.005 – 0.500 mg/kg) and by standard addition (0.010 – 0.200 mg/kg). Correlation coefficients for most pesticides, in all four matrixes, were over 0.99. Linearity for OTA quantification in grapes was performed in acetonitrile and in the sample matrixes (0.001 – 0.050 μg/ml) and in acetonitrile and in wine matrixes (0.0005 – 0.010 μg/ml).
AB  - Razvijena je brza, selektivna i osetljiva tečno-hromatografska metoda sa masenom spektrometrijom (LC-MS/MS) za istovremeno kvantitativno određivanje ostataka 41 aktivne supstance pesticida i ohratoksina A (OTA) u grožđu i vinu. Zbog povećanja osetljivosti masenog spektrometra za ispitivane analite postavljena multirezidualna metoda (MRM) je prebačena u vremenski segmentisanu metodu, tzv. metodu u dinamičkom modu (dMRM). Ekstrakcija i prečišćavanje analita iz uzoraka grožđa i vina su rađene modifikovanom Quick Easy Cheap Effective Rugged and Safe (QuEChERS) metodom. Za analizu OTA acetonitrilni ekstrakt je direktno analiziran LC-MS/MS tehnikom, dok je prečišćavanje ekstrakta za analizu ostataka rađeno pomoću kolona za disperziju na čvrstoj fazi za pigmentisane uzorke (crno grožđe i crveno vino) i za voće i povrće (belo grožđe i belo vino). Za detekciju OTA uslovi masenog spektrometra su optimizovani u cilju povećanja selektivnosti izborom jona sa najosetljivijim odgovorom (m/z 404  239 i m/z 404  221) za kvantifikaciju i identifikaciju. Za detekciju aktivnih supstanci pesticida akvizicioni parametri masenog spektrometra, SRM prelazi, fragmentacione energije i kolizione energije su preuzete iz baze podataka. U cilju razvoja metode ispitani su parametri linearnost odgovora detektora, granica detekcije (LOD), granica merenja (LOQ), matriks efekat, tačnost, preciznost i pouzdanost, a dobijene vrednosti su upoređene sa kriterijumima Uredbe 401/2006 za OTA i SANTE/11813/2017 dokumenta za aktivne supstance pesticida. Linearnost metode za ostatake u grožđu i vinu je ispitivana u mobilnoj fazi i matriksima uzoraka (0,005 do 0,500 mg/kg) i metodom standardnog dodatka (0,010 - 0,200 mg/kg)...
PB  - Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Simultaneous determination of ochratoxin a and pesticide residues in grapes and wine by LC-MS/MS
T1  - Istovremeno određivanje ohratoksina A i ostataka pesticida u grožđu i vinu LC-MS/MS tehnikom
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11478
ER  - 

@phdthesis{
author = "Špirović-Trifunović, Bojana",
year = "2019",
abstract = "A fast, selective and sensitive reversed-phased liquid chromatography with tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous quantification of 41 pesticide residues and ochratoxin A (ОТА) in grapes and wine. For increasing sensitivity of mass spectrometer multiple reaction monitoring (MRM) was transferred into time segmented method, dynamic multiple reaction monitoring (dMRM). Grape and wine extraction and purification for both, pesticide residues and OTA, were performed with a modified Quick Easy Cheap Effective Rugged and Safe (QuEChERS) method. For OTA LC-MS/MS analysis acetonitrile extract was directly injected into system, and for pesticide analysis extracts were purified with dispersive solid phase extraction for pigmented samples (black grapes and red wine) and for fruits and vegetables (white grapes and wine). MS/MS conditions were optimized in order to increase selectivity, selecting the corresponding product ions (precursor-to-fragment m/z 404 → 239 and m/z 404 → m/z 221) for quantification and identification. For pesticide residues, MS/MS aquisition parameters, SRM transitions, fragment and colision energies, from database were used. For method validation, the performance of the method was assessed and compared with the European Commission (EC) Regulations 401/2006 for OTA and with the SANTE/11813/2017 document for pesticide residues, by studying the linearity, limit of detection (LOD), limit of quantification (LOQ), matrix effect, accuracy and precision. Linearity for quantification pesticide residues in grapes and wine was performed in mobile phase and in sample matrixes (0.005 – 0.500 mg/kg) and by standard addition (0.010 – 0.200 mg/kg). Correlation coefficients for most pesticides, in all four matrixes, were over 0.99. Linearity for OTA quantification in grapes was performed in acetonitrile and in the sample matrixes (0.001 – 0.050 μg/ml) and in acetonitrile and in wine matrixes (0.0005 – 0.010 μg/ml)., Razvijena je brza, selektivna i osetljiva tečno-hromatografska metoda sa masenom spektrometrijom (LC-MS/MS) za istovremeno kvantitativno određivanje ostataka 41 aktivne supstance pesticida i ohratoksina A (OTA) u grožđu i vinu. Zbog povećanja osetljivosti masenog spektrometra za ispitivane analite postavljena multirezidualna metoda (MRM) je prebačena u vremenski segmentisanu metodu, tzv. metodu u dinamičkom modu (dMRM). Ekstrakcija i prečišćavanje analita iz uzoraka grožđa i vina su rađene modifikovanom Quick Easy Cheap Effective Rugged and Safe (QuEChERS) metodom. Za analizu OTA acetonitrilni ekstrakt je direktno analiziran LC-MS/MS tehnikom, dok je prečišćavanje ekstrakta za analizu ostataka rađeno pomoću kolona za disperziju na čvrstoj fazi za pigmentisane uzorke (crno grožđe i crveno vino) i za voće i povrće (belo grožđe i belo vino). Za detekciju OTA uslovi masenog spektrometra su optimizovani u cilju povećanja selektivnosti izborom jona sa najosetljivijim odgovorom (m/z 404  239 i m/z 404  221) za kvantifikaciju i identifikaciju. Za detekciju aktivnih supstanci pesticida akvizicioni parametri masenog spektrometra, SRM prelazi, fragmentacione energije i kolizione energije su preuzete iz baze podataka. U cilju razvoja metode ispitani su parametri linearnost odgovora detektora, granica detekcije (LOD), granica merenja (LOQ), matriks efekat, tačnost, preciznost i pouzdanost, a dobijene vrednosti su upoređene sa kriterijumima Uredbe 401/2006 za OTA i SANTE/11813/2017 dokumenta za aktivne supstance pesticida. Linearnost metode za ostatake u grožđu i vinu je ispitivana u mobilnoj fazi i matriksima uzoraka (0,005 do 0,500 mg/kg) i metodom standardnog dodatka (0,010 - 0,200 mg/kg)...",
publisher = "Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Simultaneous determination of ochratoxin a and pesticide residues in grapes and wine by LC-MS/MS, Istovremeno određivanje ohratoksina A i ostataka pesticida u grožđu i vinu LC-MS/MS tehnikom",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11478"
}

Špirović-Trifunović, B.. (2019). Simultaneous determination of ochratoxin a and pesticide residues in grapes and wine by LC-MS/MS. 
Univerzitet u Beogradu, Poljoprivredni fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_11478

Špirović-Trifunović B. Simultaneous determination of ochratoxin a and pesticide residues in grapes and wine by LC-MS/MS. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11478 .

Špirović-Trifunović, Bojana, "Simultaneous determination of ochratoxin a and pesticide residues in grapes and wine by LC-MS/MS" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11478 .

TY  - JOUR
AU  - Barać, Miroljub
AU  - Kresojević, Mirjana
AU  - Špirović-Trifunović, Bojana
AU  - Pešić, Mirjana
AU  - Vučić, Tanja
AU  - Kostić, Aleksandar
AU  - Despotović, Saša
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4739
AB  - This study deals with fatty acid profiles and mineral contents of traditional Serbian white cheeses in brine. Study covered four Serbian traditional white brined cheeses: Zlatar cheese, Sjenica cheese, Svrljig cheese and Homolje cheese prepared from raw cow and ovine milk. Fatty acid profiles of traditional cheeses were qualitatively and quantitatively different. The content of saturated fatty acids was in the range of 65.97 %-76.61 % (cow cheeses) and 69.68 %-74.52 % (ovine cheeses). Unsaturated fatty acids range of cow and ovine cheeses was 23.39 %-34.03 % and 25.48 %-30.08 %, respectively. Depending on the type of cheese, the ratio of polyunsaturated fatty acids was 1.66 %-11.03 %. The lowest content of saturated fatty acids was observed in Sjenica cow cheeses. Thus, this type of traditional cheeses had the most favorable health indices including atherogenicity index (1.89), thrombogenicity index (1.37) and desirable fatty acid ratio (46.34 %). Mineral content of traditional cheeses was also different. The ratio Ca:P was in the range of 0.70-1.33. The highest level of Ca, P, K, Mg, Zn, Cu and Cr was detected in Sjenica cheeses prepared from cow milk. The absence of Cd was observed in all cheeses.
PB  - Croatian Dairy Union, Zagreb
T2  - Mljekarstvo
T1  - Fatty acid profiles and mineral content of Serbian traditional white brined cheeses
EP  - 45
IS  - 1
SP  - 37
VL  - 68
DO  - 10.15567/mljekarstvo.2018.0105
ER  - 

@article{
author = "Barać, Miroljub and Kresojević, Mirjana and Špirović-Trifunović, Bojana and Pešić, Mirjana and Vučić, Tanja and Kostić, Aleksandar and Despotović, Saša",
year = "2018",
abstract = "This study deals with fatty acid profiles and mineral contents of traditional Serbian white cheeses in brine. Study covered four Serbian traditional white brined cheeses: Zlatar cheese, Sjenica cheese, Svrljig cheese and Homolje cheese prepared from raw cow and ovine milk. Fatty acid profiles of traditional cheeses were qualitatively and quantitatively different. The content of saturated fatty acids was in the range of 65.97 %-76.61 % (cow cheeses) and 69.68 %-74.52 % (ovine cheeses). Unsaturated fatty acids range of cow and ovine cheeses was 23.39 %-34.03 % and 25.48 %-30.08 %, respectively. Depending on the type of cheese, the ratio of polyunsaturated fatty acids was 1.66 %-11.03 %. The lowest content of saturated fatty acids was observed in Sjenica cow cheeses. Thus, this type of traditional cheeses had the most favorable health indices including atherogenicity index (1.89), thrombogenicity index (1.37) and desirable fatty acid ratio (46.34 %). Mineral content of traditional cheeses was also different. The ratio Ca:P was in the range of 0.70-1.33. The highest level of Ca, P, K, Mg, Zn, Cu and Cr was detected in Sjenica cheeses prepared from cow milk. The absence of Cd was observed in all cheeses.",
publisher = "Croatian Dairy Union, Zagreb",
journal = "Mljekarstvo",
title = "Fatty acid profiles and mineral content of Serbian traditional white brined cheeses",
pages = "45-37",
number = "1",
volume = "68",
doi = "10.15567/mljekarstvo.2018.0105"
}

Barać, M., Kresojević, M., Špirović-Trifunović, B., Pešić, M., Vučić, T., Kostić, A.,& Despotović, S.. (2018). Fatty acid profiles and mineral content of Serbian traditional white brined cheeses. in Mljekarstvo
Croatian Dairy Union, Zagreb., 68(1), 37-45.
https://doi.org/10.15567/mljekarstvo.2018.0105

Barać M, Kresojević M, Špirović-Trifunović B, Pešić M, Vučić T, Kostić A, Despotović S. Fatty acid profiles and mineral content of Serbian traditional white brined cheeses. in Mljekarstvo. 2018;68(1):37-45.
doi:10.15567/mljekarstvo.2018.0105 .

Barać, Miroljub, Kresojević, Mirjana, Špirović-Trifunović, Bojana, Pešić, Mirjana, Vučić, Tanja, Kostić, Aleksandar, Despotović, Saša, "Fatty acid profiles and mineral content of Serbian traditional white brined cheeses" in Mljekarstvo, 68, no. 1 (2018):37-45,
https://doi.org/10.15567/mljekarstvo.2018.0105 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Mačukanović-Jocić, Marina
AU  - Špirović-Trifunović, Bojana
AU  - Vukašinović, Ivana
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4421
AB  - The aim of this work was to identify and quantify fatty acids presented in pollen samples collected from six different Serbian maize hybrids by GC capillary method. Due to great importance of fatty acids as food component potential nutritional value of maize pollen as food supplement in human diet was determined. It has been shown that pollen is a great source of different fatty acids, especially unsaturated fatty acids. In total, twenty eight fatty acids were quantify - the most abundant saturated FAs were palmitic and henicosanoic acid; the most prevalent monounsaturated FAs were oleic, elaidic and cis-10-heptadecenoic acid. Linoleic and cis-11,14-eicosadienoic acid were the most abundant polyunsaturated fatty acid. Also, it was found that FAs composition was significantly influenced by the type of maize hybrids. According to nutritional recommendations, four of six pollen samples had good nutritional quality with unsaturated/saturated fatty acids ratio higher than 1.6, but there is unsatisfied distribution of omega-6 and omega-3 fatty acids as the most important type of unsaturated fatty acids.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Cereal Science
T1  - Fatty acids of maize pollen - Quantification, nutritional and morphological evaluation
EP  - 185
SP  - 180
VL  - 77
DO  - 10.1016/j.jcs.2017.08.004
ER  - 

@article{
author = "Kostić, Aleksandar and Mačukanović-Jocić, Marina and Špirović-Trifunović, Bojana and Vukašinović, Ivana and Pavlović, Vladimir and Pešić, Mirjana",
year = "2017",
abstract = "The aim of this work was to identify and quantify fatty acids presented in pollen samples collected from six different Serbian maize hybrids by GC capillary method. Due to great importance of fatty acids as food component potential nutritional value of maize pollen as food supplement in human diet was determined. It has been shown that pollen is a great source of different fatty acids, especially unsaturated fatty acids. In total, twenty eight fatty acids were quantify - the most abundant saturated FAs were palmitic and henicosanoic acid; the most prevalent monounsaturated FAs were oleic, elaidic and cis-10-heptadecenoic acid. Linoleic and cis-11,14-eicosadienoic acid were the most abundant polyunsaturated fatty acid. Also, it was found that FAs composition was significantly influenced by the type of maize hybrids. According to nutritional recommendations, four of six pollen samples had good nutritional quality with unsaturated/saturated fatty acids ratio higher than 1.6, but there is unsatisfied distribution of omega-6 and omega-3 fatty acids as the most important type of unsaturated fatty acids.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Cereal Science",
title = "Fatty acids of maize pollen - Quantification, nutritional and morphological evaluation",
pages = "185-180",
volume = "77",
doi = "10.1016/j.jcs.2017.08.004"
}

Kostić, A., Mačukanović-Jocić, M., Špirović-Trifunović, B., Vukašinović, I., Pavlović, V.,& Pešić, M.. (2017). Fatty acids of maize pollen - Quantification, nutritional and morphological evaluation. in Journal of Cereal Science
Academic Press Ltd- Elsevier Science Ltd, London., 77, 180-185.
https://doi.org/10.1016/j.jcs.2017.08.004

Kostić A, Mačukanović-Jocić M, Špirović-Trifunović B, Vukašinović I, Pavlović V, Pešić M. Fatty acids of maize pollen - Quantification, nutritional and morphological evaluation. in Journal of Cereal Science. 2017;77:180-185.
doi:10.1016/j.jcs.2017.08.004 .

Kostić, Aleksandar, Mačukanović-Jocić, Marina, Špirović-Trifunović, Bojana, Vukašinović, Ivana, Pavlović, Vladimir, Pešić, Mirjana, "Fatty acids of maize pollen - Quantification, nutritional and morphological evaluation" in Journal of Cereal Science, 77 (2017):180-185,
https://doi.org/10.1016/j.jcs.2017.08.004 . .

TY  - JOUR
AU  - Tamaš, Nenad
AU  - Dojnov, Biljana
AU  - Margetić, Aleksandra
AU  - Vujcić, Miroslava
AU  - Špirović, Bojana
AU  - Miletić, Novica
AU  - Stević, Milan
AU  - Vujcić, Zoran
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3785
AB  - Introduction. Aphis pomi (De Geer) has developed resistance to organophosphate and carbamate insecticides, as a result of long-term application of these insecticides in conventional apple orchards. For many years, the only mechanism of resistance identified in aphids was overproduction of insecticide-detoxifying esterases. Materials and methods. Insecticide resistance of A. pomi, collected from two conventional apple orchards (localities of Radmilovac-RA and Bela Crkva-BC) and one organic apple orchard (locality of Surcin-SU), was tested by bioassays and biochemical assays. Results and discussion. Compared with LC50 values for the susceptible population (organic orchard), both populations from the conventional orchards were highly resistant to pirimicarb (234.5 and 52.9 times) and moderately resistant to dimethoate (10.7 and 9.0 times). Increased esterase activity was determined in these two resistant aphid populations. Each of them also produced one esterase isoform more than the susceptible population, when 1-naphthyl acetate was used as a substrate for zymographic detection; when 2-naphthyl acetate was used as a substrate, only one resistant population produced two new esterase isoforms. In one of the resistant populations acetylcholinesterase (AChE) was significantly less inhibited by pirimicarb than in the other resistant population and the susceptible population, which indicates that this population developed another resistance mechanism-Modification of AChE (MACE). Conclusion. Detoxification of insecticides by the metabolic resistance mechanism of esterase enzymes and mechanism of modification of AChE was proven in one aphid population (RA). The other population (BC) has developed only metabolic resistance (enhanced metabolism by esterases), without modification of the insecticide target site (AChE). Development of insecticide resistance was caused by long-term application of acetylcholinesterase inhibitors (organophosphates and carbamates) in these conventional orchards.
PB  - Edp Sciences S A, Les Ulis Cedex A
T2  - Fruits
T1  - Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)
EP  - 142
IS  - 3
SP  - 135
VL  - 70
DO  - 10.1051/fruits/2015005
ER  - 

@article{
author = "Tamaš, Nenad and Dojnov, Biljana and Margetić, Aleksandra and Vujcić, Miroslava and Špirović, Bojana and Miletić, Novica and Stević, Milan and Vujcić, Zoran",
year = "2015",
abstract = "Introduction. Aphis pomi (De Geer) has developed resistance to organophosphate and carbamate insecticides, as a result of long-term application of these insecticides in conventional apple orchards. For many years, the only mechanism of resistance identified in aphids was overproduction of insecticide-detoxifying esterases. Materials and methods. Insecticide resistance of A. pomi, collected from two conventional apple orchards (localities of Radmilovac-RA and Bela Crkva-BC) and one organic apple orchard (locality of Surcin-SU), was tested by bioassays and biochemical assays. Results and discussion. Compared with LC50 values for the susceptible population (organic orchard), both populations from the conventional orchards were highly resistant to pirimicarb (234.5 and 52.9 times) and moderately resistant to dimethoate (10.7 and 9.0 times). Increased esterase activity was determined in these two resistant aphid populations. Each of them also produced one esterase isoform more than the susceptible population, when 1-naphthyl acetate was used as a substrate for zymographic detection; when 2-naphthyl acetate was used as a substrate, only one resistant population produced two new esterase isoforms. In one of the resistant populations acetylcholinesterase (AChE) was significantly less inhibited by pirimicarb than in the other resistant population and the susceptible population, which indicates that this population developed another resistance mechanism-Modification of AChE (MACE). Conclusion. Detoxification of insecticides by the metabolic resistance mechanism of esterase enzymes and mechanism of modification of AChE was proven in one aphid population (RA). The other population (BC) has developed only metabolic resistance (enhanced metabolism by esterases), without modification of the insecticide target site (AChE). Development of insecticide resistance was caused by long-term application of acetylcholinesterase inhibitors (organophosphates and carbamates) in these conventional orchards.",
publisher = "Edp Sciences S A, Les Ulis Cedex A",
journal = "Fruits",
title = "Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)",
pages = "142-135",
number = "3",
volume = "70",
doi = "10.1051/fruits/2015005"
}

Tamaš, N., Dojnov, B., Margetić, A., Vujcić, M., Špirović, B., Miletić, N., Stević, M.,& Vujcić, Z.. (2015). Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae). in Fruits
Edp Sciences S A, Les Ulis Cedex A., 70(3), 135-142.
https://doi.org/10.1051/fruits/2015005

Tamaš N, Dojnov B, Margetić A, Vujcić M, Špirović B, Miletić N, Stević M, Vujcić Z. Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae). in Fruits. 2015;70(3):135-142.
doi:10.1051/fruits/2015005 .

Tamaš, Nenad, Dojnov, Biljana, Margetić, Aleksandra, Vujcić, Miroslava, Špirović, Bojana, Miletić, Novica, Stević, Milan, Vujcić, Zoran, "Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)" in Fruits, 70, no. 3 (2015):135-142,
https://doi.org/10.1051/fruits/2015005 . .

TY  - JOUR
AU  - Špirović-Trifunović, Bojana
AU  - Vuković, Gorica
AU  - Jovanović-Radovanov, Katarina
AU  - Bursić, Vojislava P.
AU  - Meseldžija, Maja U.
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3902
AB  - Linuron is a selective herbicide used for the control of broadleaf weeds. Its mode of action is the inhibition of photosynthesis. The QuEChERS method for extraction of linuron residues from chamomile was used. The LC-MS/MS method was used for determination of linuron residues. Its linearity was studied in a range of 0.025-0.50 μg/ml using matrix-matched calibration, and the determination coefficient (R2) was higher than 0.99. Blank chamomile samples were spiked with linuron solution at three concentration levels yielding recoveries of over 90%. The internal standard added in all samples was isoproturon-d6. There were no linuron residues in chamomile flowers, while the residues ranged from 0.010 to 0.040 mg/kg in the flower stalk samples.
AB  - Linuron je selektivni herbicid, koji se koristi za suzbijanje širokolisnih korova. Njegov mehanizam delovanja je inhibicija fotosinteze (fotosistema II). Za ekstrakciju ostataka linurona iz uzoraka kamilice korišćena je QuEChERS metoda. Određivanje nivoa ostataka linurona vršeno je tečnom hromatografijom sa masenim spektroskopijom. Linearnost metode je ispitivana u opsegu koncentracija od 0.025 - 0.50 μg/ml, korišćenjem metode kalibracije u matriksu, pri čemu je koeficijent određivanja (R2) bio veći od 0.99. Tačnost metode je ispitivana obogaćivanjem kontrolnih uzoraka kamilice na tri koncentraciona nivoa. Prinos ekstrakcije je bio preko 90 %. Interni standard korišćen za analizu je bio izoproturon-D6. U cvetu kamilice nisu nađeni ostaci linurona, dok su u uzorcima drške bili u opsegu od 0.010-0.040 mg/kg.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Determination of linuron in chamomile by LC-MS/MS using the QuEchERS extraction method
T1  - Određivanje linurona u kamilici LC-MS/MS tehnikom i QuEchERS metodom ekstrakcije
EP  - 121
IS  - 2
SP  - 115
VL  - 30
DO  - 10.2298/pif1502115S
ER  - 

@article{
author = "Špirović-Trifunović, Bojana and Vuković, Gorica and Jovanović-Radovanov, Katarina and Bursić, Vojislava P. and Meseldžija, Maja U.",
year = "2015",
abstract = "Linuron is a selective herbicide used for the control of broadleaf weeds. Its mode of action is the inhibition of photosynthesis. The QuEChERS method for extraction of linuron residues from chamomile was used. The LC-MS/MS method was used for determination of linuron residues. Its linearity was studied in a range of 0.025-0.50 μg/ml using matrix-matched calibration, and the determination coefficient (R2) was higher than 0.99. Blank chamomile samples were spiked with linuron solution at three concentration levels yielding recoveries of over 90%. The internal standard added in all samples was isoproturon-d6. There were no linuron residues in chamomile flowers, while the residues ranged from 0.010 to 0.040 mg/kg in the flower stalk samples., Linuron je selektivni herbicid, koji se koristi za suzbijanje širokolisnih korova. Njegov mehanizam delovanja je inhibicija fotosinteze (fotosistema II). Za ekstrakciju ostataka linurona iz uzoraka kamilice korišćena je QuEChERS metoda. Određivanje nivoa ostataka linurona vršeno je tečnom hromatografijom sa masenim spektroskopijom. Linearnost metode je ispitivana u opsegu koncentracija od 0.025 - 0.50 μg/ml, korišćenjem metode kalibracije u matriksu, pri čemu je koeficijent određivanja (R2) bio veći od 0.99. Tačnost metode je ispitivana obogaćivanjem kontrolnih uzoraka kamilice na tri koncentraciona nivoa. Prinos ekstrakcije je bio preko 90 %. Interni standard korišćen za analizu je bio izoproturon-D6. U cvetu kamilice nisu nađeni ostaci linurona, dok su u uzorcima drške bili u opsegu od 0.010-0.040 mg/kg.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Determination of linuron in chamomile by LC-MS/MS using the QuEchERS extraction method, Određivanje linurona u kamilici LC-MS/MS tehnikom i QuEchERS metodom ekstrakcije",
pages = "121-115",
number = "2",
volume = "30",
doi = "10.2298/pif1502115S"
}

Špirović-Trifunović, B., Vuković, G., Jovanović-Radovanov, K., Bursić, V. P.,& Meseldžija, M. U.. (2015). Determination of linuron in chamomile by LC-MS/MS using the QuEchERS extraction method. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 30(2), 115-121.
https://doi.org/10.2298/pif1502115S

Špirović-Trifunović B, Vuković G, Jovanović-Radovanov K, Bursić VP, Meseldžija MU. Determination of linuron in chamomile by LC-MS/MS using the QuEchERS extraction method. in Pesticidi i fitomedicina. 2015;30(2):115-121.
doi:10.2298/pif1502115S .

Špirović-Trifunović, Bojana, Vuković, Gorica, Jovanović-Radovanov, Katarina, Bursić, Vojislava P., Meseldžija, Maja U., "Determination of linuron in chamomile by LC-MS/MS using the QuEchERS extraction method" in Pesticidi i fitomedicina, 30, no. 2 (2015):115-121,
https://doi.org/10.2298/pif1502115S . .

TY  - JOUR
AU  - Bursić, Vojislava
AU  - Lazić, Sanja
AU  - Zeremski, Tijana
AU  - Vuković, Gorica
AU  - Špirović, Bojana
AU  - Pucarević, Mira
AU  - Stojanović, Tijana
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2915
AB  - The programmes for pesticide residues monitoring in food which are continually carried out in the world ensure that the consumers are not exposed to pesticide residues as well as that only the registered compounds are used in the production. All the member countries of the EU are obliged to observe the official control of pesticide residues in food, in accordance with the Regulations EC 396/2005. At present, in the Republic of Serbia, the programme for monitoring the pesticide residues in food is being prepared as the results of numerous analyses of random samples of fruit, in the market over the past years justify the introduction of such a programme.
AB  - Programi praćenja ostataka pesticida u hrani koji se kontinuirano sprovode u svetu osiguravaju potrošače da ne budu izloženi nepoželjnom nivou ostataka pesticida, kao i da se u proizvodnji koriste samo registrovana jedinjenja. Sve zemlje članice EU su dužne da sprovode zvaničnu kontrolu prisustva ostataka pesticida u hrani, u saglasnosti sa Regulativom EC 396/2005. U Republici Srbiji je u pripremi monitoring program za ostatke pesticida u hrani, a rezultati analiza većeg broja slučajno izabranih uzoraka voća, na našem tržištu, tokom proteklih godina ukazuju na opravdanost uvođenja ovakvog programa.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Monitoring of pesticide residues in fruit
T1  - Praćenje ostataka pesticida u voću
EP  - 430
IS  - 5
SP  - 420
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2915
ER  - 

@article{
author = "Bursić, Vojislava and Lazić, Sanja and Zeremski, Tijana and Vuković, Gorica and Špirović, Bojana and Pucarević, Mira and Stojanović, Tijana",
year = "2012",
abstract = "The programmes for pesticide residues monitoring in food which are continually carried out in the world ensure that the consumers are not exposed to pesticide residues as well as that only the registered compounds are used in the production. All the member countries of the EU are obliged to observe the official control of pesticide residues in food, in accordance with the Regulations EC 396/2005. At present, in the Republic of Serbia, the programme for monitoring the pesticide residues in food is being prepared as the results of numerous analyses of random samples of fruit, in the market over the past years justify the introduction of such a programme., Programi praćenja ostataka pesticida u hrani koji se kontinuirano sprovode u svetu osiguravaju potrošače da ne budu izloženi nepoželjnom nivou ostataka pesticida, kao i da se u proizvodnji koriste samo registrovana jedinjenja. Sve zemlje članice EU su dužne da sprovode zvaničnu kontrolu prisustva ostataka pesticida u hrani, u saglasnosti sa Regulativom EC 396/2005. U Republici Srbiji je u pripremi monitoring program za ostatke pesticida u hrani, a rezultati analiza većeg broja slučajno izabranih uzoraka voća, na našem tržištu, tokom proteklih godina ukazuju na opravdanost uvođenja ovakvog programa.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Monitoring of pesticide residues in fruit, Praćenje ostataka pesticida u voću",
pages = "430-420",
number = "5",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2915"
}

Bursić, V., Lazić, S., Zeremski, T., Vuković, G., Špirović, B., Pucarević, M.,& Stojanović, T.. (2012). Monitoring of pesticide residues in fruit. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 40(5), 420-430.
https://hdl.handle.net/21.15107/rcub_agrospace_2915

Bursić V, Lazić S, Zeremski T, Vuković G, Špirović B, Pucarević M, Stojanović T. Monitoring of pesticide residues in fruit. in Biljni lekar. 2012;40(5):420-430.
https://hdl.handle.net/21.15107/rcub_agrospace_2915 .

Bursić, Vojislava, Lazić, Sanja, Zeremski, Tijana, Vuković, Gorica, Špirović, Bojana, Pucarević, Mira, Stojanović, Tijana, "Monitoring of pesticide residues in fruit" in Biljni lekar, 40, no. 5 (2012):420-430,
https://hdl.handle.net/21.15107/rcub_agrospace_2915 .