TY  - JOUR
AU  - Cvetković, Stefana
AU  - Tenji, Dina
AU  - Mitić-Ćulafić, Dragana
AU  - Vuletić, Stefana
AU  - Ganić, Tea
AU  - Djekić, Ilija
AU  - Nikolić, Biljana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6377
AB  - As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against quorum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd
T2  - Food Bioscience
T2  - Food Bioscience
T1  - Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces
VL  - 54
DO  - 10.1016/j.fbio.2023.102857
ER  - 

@article{
author = "Cvetković, Stefana and Tenji, Dina and Mitić-Ćulafić, Dragana and Vuletić, Stefana and Ganić, Tea and Djekić, Ilija and Nikolić, Biljana",
year = "2023",
abstract = "As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against quorum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd",
journal = "Food Bioscience, Food Bioscience",
title = "Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces",
volume = "54",
doi = "10.1016/j.fbio.2023.102857"
}

Cvetković, S., Tenji, D., Mitić-Ćulafić, D., Vuletić, S., Ganić, T., Djekić, I.,& Nikolić, B.. (2023). Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces. in Food Bioscience, 54.
https://doi.org/10.1016/j.fbio.2023.102857

Cvetković S, Tenji D, Mitić-Ćulafić D, Vuletić S, Ganić T, Djekić I, Nikolić B. Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces. in Food Bioscience. 2023;54.
doi:10.1016/j.fbio.2023.102857 .

Cvetković, Stefana, Tenji, Dina, Mitić-Ćulafić, Dragana, Vuletić, Stefana, Ganić, Tea, Djekić, Ilija, Nikolić, Biljana, "Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces" in Food Bioscience, 54 (2023),
https://doi.org/10.1016/j.fbio.2023.102857 . .