TY  - CONF
AU  - Golubović-Ćurguz, Vesna
AU  - Dunisijević-Bojović, Danijela
AU  - Dukić, Matilda
AU  - Raičević, Vera
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4625
AB  - Phytoremediation and phytostabilisation of abandon open miming sites is very important process for rehabilitation of land and watercourses to an acceptable standard. Seed germination and seedling establishment in post-mining sites is of great importance for early succession and restoration patterns. Several of early colonizers are tree species, but they could be very important elements of early successions and influence dynamic of early revegetation in disturbed areas. Spontaneous colonization of mining spoils is difficult due to extremely difficult condition such as extremely low or high pH, mineral soil, low water capacity and nutrient deficiency. Besides this, high concentration and solubility of toxic metals in mining areas could be the most limiting factor for plant establishment. We analyzed parameters of seed germination and seedling growth of early successional tree species (two broad leaf and two pine species species against): Ailanthus altissima, Acer negundo, Pinus silvestris, Picea abies in several experimental systems with three different concentration of toxic metals (Pb, Cd, Zn) to assess influence of metal toxicity in early phase of plant development. Lead in applied concentrations (25, 50 and 100 mu M) did not affect significantly the seed germination percentage in Ailanthus altissima and Acer negundo while cadmium in highest concentrations (100 mu M) caused reduction of the germination percentage, germination index and vigor index in both species. Analysis of the parameters of early seedling development indicates the most pronounced toxic effects of cadmium. In Ailanthus altissima Zn treatments caused significant inhibition of shoot growth and repress development of assimilating organs in concentrations of 25, 100 and 250 mu M. In concentration of 25 mu M Zn caused an inhibition of the root growth. The spruce and Scot pine seeds which were developed on substrates with addition of heavy metals solutions showed significant tolerance to presence of all metals, but percentage of germinating seeds depended on the type of metal and its concentration. The seed of P. abies demonstrated higher tolerance to presence of heavy metals in lower concentrations, then seed of Pinus sylvestris and germinated in all variants. In the conditions of highest concentrations, the seeds of both species did not sprout regardless of the type of metal. The P. abies seedlings were very tolerant to the presence of all metals. The highest concentrations of cadmium had a significant influence on the decrease of the number of the seedlings which survived, as well as on the decrease of biomass in comparison to lead. Zinc had the least adverse effect on the growth and survival of seedlings. P. sylvestris seedlings did not exhibit the same tolerance for the presence of heavy metals.
PB  - Catena Verlag, Reiskirchen
C3  - Soil and Water Resources Protection in the Changing Environment
T1  - Seed germination and seedling growth of Ailanthus altissima, Acer negundo, Pinus silvestris, and Picea abies in elevated concentrations of Zn, Pb and Cd under laboratory conditions
EP  - 199
SP  - 188
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4625
ER  - 

@conference{
author = "Golubović-Ćurguz, Vesna and Dunisijević-Bojović, Danijela and Dukić, Matilda and Raičević, Vera",
year = "2018",
abstract = "Phytoremediation and phytostabilisation of abandon open miming sites is very important process for rehabilitation of land and watercourses to an acceptable standard. Seed germination and seedling establishment in post-mining sites is of great importance for early succession and restoration patterns. Several of early colonizers are tree species, but they could be very important elements of early successions and influence dynamic of early revegetation in disturbed areas. Spontaneous colonization of mining spoils is difficult due to extremely difficult condition such as extremely low or high pH, mineral soil, low water capacity and nutrient deficiency. Besides this, high concentration and solubility of toxic metals in mining areas could be the most limiting factor for plant establishment. We analyzed parameters of seed germination and seedling growth of early successional tree species (two broad leaf and two pine species species against): Ailanthus altissima, Acer negundo, Pinus silvestris, Picea abies in several experimental systems with three different concentration of toxic metals (Pb, Cd, Zn) to assess influence of metal toxicity in early phase of plant development. Lead in applied concentrations (25, 50 and 100 mu M) did not affect significantly the seed germination percentage in Ailanthus altissima and Acer negundo while cadmium in highest concentrations (100 mu M) caused reduction of the germination percentage, germination index and vigor index in both species. Analysis of the parameters of early seedling development indicates the most pronounced toxic effects of cadmium. In Ailanthus altissima Zn treatments caused significant inhibition of shoot growth and repress development of assimilating organs in concentrations of 25, 100 and 250 mu M. In concentration of 25 mu M Zn caused an inhibition of the root growth. The spruce and Scot pine seeds which were developed on substrates with addition of heavy metals solutions showed significant tolerance to presence of all metals, but percentage of germinating seeds depended on the type of metal and its concentration. The seed of P. abies demonstrated higher tolerance to presence of heavy metals in lower concentrations, then seed of Pinus sylvestris and germinated in all variants. In the conditions of highest concentrations, the seeds of both species did not sprout regardless of the type of metal. The P. abies seedlings were very tolerant to the presence of all metals. The highest concentrations of cadmium had a significant influence on the decrease of the number of the seedlings which survived, as well as on the decrease of biomass in comparison to lead. Zinc had the least adverse effect on the growth and survival of seedlings. P. sylvestris seedlings did not exhibit the same tolerance for the presence of heavy metals.",
publisher = "Catena Verlag, Reiskirchen",
journal = "Soil and Water Resources Protection in the Changing Environment",
title = "Seed germination and seedling growth of Ailanthus altissima, Acer negundo, Pinus silvestris, and Picea abies in elevated concentrations of Zn, Pb and Cd under laboratory conditions",
pages = "199-188",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4625"
}

Golubović-Ćurguz, V., Dunisijević-Bojović, D., Dukić, M.,& Raičević, V.. (2018). Seed germination and seedling growth of Ailanthus altissima, Acer negundo, Pinus silvestris, and Picea abies in elevated concentrations of Zn, Pb and Cd under laboratory conditions. in Soil and Water Resources Protection in the Changing Environment
Catena Verlag, Reiskirchen., 45, 188-199.
https://hdl.handle.net/21.15107/rcub_agrospace_4625

Golubović-Ćurguz V, Dunisijević-Bojović D, Dukić M, Raičević V. Seed germination and seedling growth of Ailanthus altissima, Acer negundo, Pinus silvestris, and Picea abies in elevated concentrations of Zn, Pb and Cd under laboratory conditions. in Soil and Water Resources Protection in the Changing Environment. 2018;45:188-199.
https://hdl.handle.net/21.15107/rcub_agrospace_4625 .

Golubović-Ćurguz, Vesna, Dunisijević-Bojović, Danijela, Dukić, Matilda, Raičević, Vera, "Seed germination and seedling growth of Ailanthus altissima, Acer negundo, Pinus silvestris, and Picea abies in elevated concentrations of Zn, Pb and Cd under laboratory conditions" in Soil and Water Resources Protection in the Changing Environment, 45 (2018):188-199,
https://hdl.handle.net/21.15107/rcub_agrospace_4625 .

TY  - JOUR
AU  - Skocajić, Dragana M.
AU  - Nesić, Marija M.
AU  - Nonić, Marina Z.
AU  - Fotirić-Akšić, Milica
AU  - Grbić, Mihailo N.
AU  - Dukić, Matilda S.
AU  - Sijacić-Nikolić, Mirjana T.
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4490
AB  - Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.
PB  - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
T2  - NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
T1  - In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks
EP  - 399
IS  - 2
SP  - 392
VL  - 45
DO  - 10.15835/nbha45210899
ER  - 

@article{
author = "Skocajić, Dragana M. and Nesić, Marija M. and Nonić, Marina Z. and Fotirić-Akšić, Milica and Grbić, Mihailo N. and Dukić, Matilda S. and Sijacić-Nikolić, Mirjana T.",
year = "2017",
abstract = "Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.",
publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca",
journal = "NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA",
title = "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks",
pages = "399-392",
number = "2",
volume = "45",
doi = "10.15835/nbha45210899"
}

Skocajić, D. M., Nesić, M. M., Nonić, M. Z., Fotirić-Akšić, M., Grbić, M. N., Dukić, M. S.,& Sijacić-Nikolić, M. T.. (2017). In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 45(2), 392-399.
https://doi.org/10.15835/nbha45210899

Skocajić DM, Nesić MM, Nonić MZ, Fotirić-Akšić M, Grbić MN, Dukić MS, Sijacić-Nikolić MT. In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA. 2017;45(2):392-399.
doi:10.15835/nbha45210899 .

Skocajić, Dragana M., Nesić, Marija M., Nonić, Marina Z., Fotirić-Akšić, Milica, Grbić, Mihailo N., Dukić, Matilda S., Sijacić-Nikolić, Mirjana T., "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks" in NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, 45, no. 2 (2017):392-399,
https://doi.org/10.15835/nbha45210899 . .