TY  - JOUR
AU  - Puvača, Nikola
AU  - Ljubojević Pelić, Dragana
AU  - Pelić, Miloš
AU  - Bursić, Vojislava
AU  - Tufarelli, Vincenzo
AU  - Piemontese, Luca
AU  - Vuković, Gorica
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6270
AB  - The aim of this research was to investigate the effects of biofilm on antibiotic resistance of the bacterial isolates present in fish meat and to assess the risk of antibiotic residues for public health. Common carp, silver carp and grass carp fishes were purchased from retail stores for an in vitro biofilm investigation and a drug-resistant pattern determination. In all samples, up to 104 CFU/g of bacteria, such as Escherichia coli, Aeromonas hydrophila, Shewanella putrefaciens, Vibrio spp. and Staphylococcus spp., were observed. Isolates from the samples and their biofilms were subjected to an antibiogram assay using antibiotics such as amoxicillin, ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin, streptomycin, tetracycline and trimethoprim. Obtained results showed that some of the isolates were sensitive to antibiotics and some were resistant. Results of LC-MS/MS analysis showed that antibiotics residues were present in fish samples in the range between 4.9 and 199.4 µg/kg, with a total sum of 417.1 µg/kg. Estimated daily intake (EDI) was established to be 0.274 μg/kg of body weight/day for men and 0.332 μg/kg of body weight/day for women, with an acceptable daily intake (ADI) of 8.5 and 7.0 µg/kg of body weight/day for men and women, respectively. The results of the present study, therefore, highlight the safe consumption of fresh fish. © 2023 by the authors.
T2  - Antibiotics
T2  - Antibiotics
T1  - Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health
IS  - 1
VL  - 12
DO  - 10.3390/antibiotics12010143
ER  - 

@article{
author = "Puvača, Nikola and Ljubojević Pelić, Dragana and Pelić, Miloš and Bursić, Vojislava and Tufarelli, Vincenzo and Piemontese, Luca and Vuković, Gorica",
year = "2023",
abstract = "The aim of this research was to investigate the effects of biofilm on antibiotic resistance of the bacterial isolates present in fish meat and to assess the risk of antibiotic residues for public health. Common carp, silver carp and grass carp fishes were purchased from retail stores for an in vitro biofilm investigation and a drug-resistant pattern determination. In all samples, up to 104 CFU/g of bacteria, such as Escherichia coli, Aeromonas hydrophila, Shewanella putrefaciens, Vibrio spp. and Staphylococcus spp., were observed. Isolates from the samples and their biofilms were subjected to an antibiogram assay using antibiotics such as amoxicillin, ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin, streptomycin, tetracycline and trimethoprim. Obtained results showed that some of the isolates were sensitive to antibiotics and some were resistant. Results of LC-MS/MS analysis showed that antibiotics residues were present in fish samples in the range between 4.9 and 199.4 µg/kg, with a total sum of 417.1 µg/kg. Estimated daily intake (EDI) was established to be 0.274 μg/kg of body weight/day for men and 0.332 μg/kg of body weight/day for women, with an acceptable daily intake (ADI) of 8.5 and 7.0 µg/kg of body weight/day for men and women, respectively. The results of the present study, therefore, highlight the safe consumption of fresh fish. © 2023 by the authors.",
journal = "Antibiotics, Antibiotics",
title = "Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health",
number = "1",
volume = "12",
doi = "10.3390/antibiotics12010143"
}

Puvača, N., Ljubojević Pelić, D., Pelić, M., Bursić, V., Tufarelli, V., Piemontese, L.,& Vuković, G.. (2023). Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health. in Antibiotics, 12(1).
https://doi.org/10.3390/antibiotics12010143

Puvača N, Ljubojević Pelić D, Pelić M, Bursić V, Tufarelli V, Piemontese L, Vuković G. Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health. in Antibiotics. 2023;12(1).
doi:10.3390/antibiotics12010143 .

Puvača, Nikola, Ljubojević Pelić, Dragana, Pelić, Miloš, Bursić, Vojislava, Tufarelli, Vincenzo, Piemontese, Luca, Vuković, Gorica, "Microbial Resistance to Antibiotics and Biofilm Formation of Bacterial Isolates from Different Carp Species and Risk Assessment for Public Health" in Antibiotics, 12, no. 1 (2023),
https://doi.org/10.3390/antibiotics12010143 . .

TY  - CONF
AU  - Babić, Jelena
AU  - Petrović, Jelena
AU  - Kartalović, Brankica
AU  - Pelić, Miloš
AU  - Jaksić, Sandra
AU  - Ćirković, Miroslav
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5609
AB  - Republika Srbija nema sopstvenu proizvodnju morskih riba i poseduje svega nekoliko objekata za preradu morske ribe. Sva morska riba se uvozi, samim tim su i sve konzerve od komada i komadića tunja na našem tržištu uvezene. Uvoz konzervi od tunja, kao i svih drugih ribljih proizvoda prilikom ulaska u našu zemlju se mora podvrgnuti kontroli granične veterinarske inspekcije. Odmah nakon prispeća pošiljke na granični prelaz lice odgovorno za pošiljku, pored obrasca zajedničkog veterinarskog ulaznog dokumenta za proizvode, podnosi i zahtev za veterinarsko sanitarni pregled. Svaka pošiljka konzervi od tunja u uvozu u Republiku Srbiju mora biti podvrgnuta pregledu dokumentacije, identifikaciji pošiljke i fizičkom, hemijskom, mikrobiološkom i senzorskom pregledu.
Sadržaj histamina u ribama i proizvodima od riba je pre svega kriterijum bezbednosti hrane obzirom da utiče na zdravlje ljudi, ali je u isto vreme i pokazatelj higijene procesa i svežine proizvoda. Cilj ove studije je da se obezbede informacije o prisustvu histamina u uvezenim konzervama od tunja i da se ukaže na značaj granične inspekcije u kontroli histaminske intoksikacije koja nastaje kao posledica konzumiranja konzervi od tunja sa povišenim sadržajem histamina. 
Tokom jednogodišnje studije (od januara do decembra 2014. godine) ispitano je 97 proizvodnih partija uvezenih konzervi od tunja. Svih 97 proizvodnih partija je uzorkovano tokom granične inspekcije i ispitano u laboratorijama Naučnog instituta za veterinarstvo „Novi Sad“. Ispitivanje sadržaja prisustva histamina u konzervama od tunja je urađeno ELISA metodom sa test kitom HIS-E02 (Immunolab GmbH, Germany).  
Rezultati studije pokazuju da je 3,09 % proizvodnih partija uvezenih konzervi od tunja imalo nezadovoljavajuće povećan nivo sadržaja histamina i da nisu bezbedne za ljudsku ishranu. Sve proizvodne partije kod kojih je dokazan povećan nivo histamina, odnosno koje su proglašene za nebezbedne za ljudsku ishranu su vraćene, a uvoz je zabranjen. Granična inspekcija, tj. ispitivanje pri graničnoj inspekcije predstavlja značajnu preventivnu meru u sprečavanju histaminske intoksikacije kao posledice konzumiranja konzervi od tunja sa povišenim sadržajem histamina.
C3  - 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10
T1  - The Importance Of Border Inspection In Control Histamine Poisoning From Canned Tuna
T1  - Značaj granične inspekcije u kontroli trovanja histaminom iz konzervi od tuna
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5609
ER  - 

@conference{
author = "Babić, Jelena and Petrović, Jelena and Kartalović, Brankica and Pelić, Miloš and Jaksić, Sandra and Ćirković, Miroslav",
year = "2015",
abstract = "Republika Srbija nema sopstvenu proizvodnju morskih riba i poseduje svega nekoliko objekata za preradu morske ribe. Sva morska riba se uvozi, samim tim su i sve konzerve od komada i komadića tunja na našem tržištu uvezene. Uvoz konzervi od tunja, kao i svih drugih ribljih proizvoda prilikom ulaska u našu zemlju se mora podvrgnuti kontroli granične veterinarske inspekcije. Odmah nakon prispeća pošiljke na granični prelaz lice odgovorno za pošiljku, pored obrasca zajedničkog veterinarskog ulaznog dokumenta za proizvode, podnosi i zahtev za veterinarsko sanitarni pregled. Svaka pošiljka konzervi od tunja u uvozu u Republiku Srbiju mora biti podvrgnuta pregledu dokumentacije, identifikaciji pošiljke i fizičkom, hemijskom, mikrobiološkom i senzorskom pregledu.
Sadržaj histamina u ribama i proizvodima od riba je pre svega kriterijum bezbednosti hrane obzirom da utiče na zdravlje ljudi, ali je u isto vreme i pokazatelj higijene procesa i svežine proizvoda. Cilj ove studije je da se obezbede informacije o prisustvu histamina u uvezenim konzervama od tunja i da se ukaže na značaj granične inspekcije u kontroli histaminske intoksikacije koja nastaje kao posledica konzumiranja konzervi od tunja sa povišenim sadržajem histamina. 
Tokom jednogodišnje studije (od januara do decembra 2014. godine) ispitano je 97 proizvodnih partija uvezenih konzervi od tunja. Svih 97 proizvodnih partija je uzorkovano tokom granične inspekcije i ispitano u laboratorijama Naučnog instituta za veterinarstvo „Novi Sad“. Ispitivanje sadržaja prisustva histamina u konzervama od tunja je urađeno ELISA metodom sa test kitom HIS-E02 (Immunolab GmbH, Germany).  
Rezultati studije pokazuju da je 3,09 % proizvodnih partija uvezenih konzervi od tunja imalo nezadovoljavajuće povećan nivo sadržaja histamina i da nisu bezbedne za ljudsku ishranu. Sve proizvodne partije kod kojih je dokazan povećan nivo histamina, odnosno koje su proglašene za nebezbedne za ljudsku ishranu su vraćene, a uvoz je zabranjen. Granična inspekcija, tj. ispitivanje pri graničnoj inspekcije predstavlja značajnu preventivnu meru u sprečavanju histaminske intoksikacije kao posledice konzumiranja konzervi od tunja sa povišenim sadržajem histamina.",
journal = "7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10",
title = "The Importance Of Border Inspection In Control Histamine Poisoning From Canned Tuna, Značaj granične inspekcije u kontroli trovanja histaminom iz konzervi od tuna",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5609"
}

Babić, J., Petrović, J., Kartalović, B., Pelić, M., Jaksić, S.,& Ćirković, M.. (2015). The Importance Of Border Inspection In Control Histamine Poisoning From Canned Tuna. in 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10.
https://hdl.handle.net/21.15107/rcub_agrospace_5609

Babić J, Petrović J, Kartalović B, Pelić M, Jaksić S, Ćirković M. The Importance Of Border Inspection In Control Histamine Poisoning From Canned Tuna. in 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10. 2015;.
https://hdl.handle.net/21.15107/rcub_agrospace_5609 .

Babić, Jelena, Petrović, Jelena, Kartalović, Brankica, Pelić, Miloš, Jaksić, Sandra, Ćirković, Miroslav, "The Importance Of Border Inspection In Control Histamine Poisoning From Canned Tuna" in 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10 (2015),
https://hdl.handle.net/21.15107/rcub_agrospace_5609 .