TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - van Griensven, Leo
AU  - Jakovljevic, Dragica
AU  - Todorovic, Nina
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Vunduk, Jovana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6269
AB  - Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet. These valuable organisms are more than food, they fit in perfectly as a novel market group known as nutra-mycoceuticals. Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry. Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients. This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers, β-glucans. The literature data on their bioavailability, metabolic transformations, preclinical and human clinical research, and safety are discussed. Immunomodulatory mechanisms of polyphenol ingredients are also considered. These molecules present great potential in the design of the new immunity balancer formulations according to their widespread structural diversity. Finally, we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity. © 2022
T2  - Food Science and Human Wellness
T2  - Food Science and Human Wellness
T1  - Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application
EP  - 396
IS  - 2
SP  - 378
VL  - 12
DO  - 10.1016/j.fshw.2022.07.040
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and van Griensven, Leo and Jakovljevic, Dragica and Todorovic, Nina and Wan-Mohtar, Wan Abd Al Qadr Imad and Vunduk, Jovana",
year = "2023",
abstract = "Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet. These valuable organisms are more than food, they fit in perfectly as a novel market group known as nutra-mycoceuticals. Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry. Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients. This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers, β-glucans. The literature data on their bioavailability, metabolic transformations, preclinical and human clinical research, and safety are discussed. Immunomodulatory mechanisms of polyphenol ingredients are also considered. These molecules present great potential in the design of the new immunity balancer formulations according to their widespread structural diversity. Finally, we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity. © 2022",
journal = "Food Science and Human Wellness, Food Science and Human Wellness",
title = "Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application",
pages = "396-378",
number = "2",
volume = "12",
doi = "10.1016/j.fshw.2022.07.040"
}

Kozarski, M., Klaus, A., van Griensven, L., Jakovljevic, D., Todorovic, N., Wan-Mohtar, W. A. A. Q. I.,& Vunduk, J.. (2023). Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application. in Food Science and Human Wellness, 12(2), 378-396.
https://doi.org/10.1016/j.fshw.2022.07.040

Kozarski M, Klaus A, van Griensven L, Jakovljevic D, Todorovic N, Wan-Mohtar WAAQI, Vunduk J. Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application. in Food Science and Human Wellness. 2023;12(2):378-396.
doi:10.1016/j.fshw.2022.07.040 .

Kozarski, Maja, Klaus, Anita, van Griensven, Leo, Jakovljevic, Dragica, Todorovic, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, Vunduk, Jovana, "Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application" in Food Science and Human Wellness, 12, no. 2 (2023):378-396,
https://doi.org/10.1016/j.fshw.2022.07.040 . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Wan‑Mohtar, Wan Abd Al Qadr Imad
AU  - Nikolić, Biljana
AU  - Cvetković, Stefana
AU  - Vunduk, Jovana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5805
AB  - Four types of mycelial extracts were derived from the airlift liquid fermentation (ALF) of Pleurotus flabellatus, namely
exopolysaccharide (EX), endopolysaccharide (EN), hot water (WE), and hot alkali (AE) extracts. Such extracts were screened
for their active components and biological potential. EN proved to be most effective in inhibition of lipid peroxidation
(
EC50 = 1.71 ± 0.02 mg/mL) and in Cupric ion reducing antioxidant capacity (CUPRAC) assay (
EC50 = 2.91 ± 0.01 mg
TE/g). AE exhibited most pronounced ability to chelate ferrous ions (
EC50 = 4.96 ± 0.08 mg/mL) and to scavenge ABTS
radicals (
EC50 = 3.36 ± 0.03 mg TE/g). β-glucans and total phenols contributed most to the chelating ability and quenching
of ABTS radicals. Inhibition of lipid peroxidation correlated best with total glucans, total proteins, and β-glucans. Total
proteins contributed most to CUPRAC antioxidant capacity. Antifungal effect was determined against Candida albicans
ATCC 10231 (MIC: 0.019–0.625 mg/mL; MFC: 0.039–2.5 mg/mL), and towards C. albicans clinical isolate (MIC and MFC:
10.0–20.0 mg/mL). Comparison of cytotoxicity against colorectal carcinoma HCT 116 cells (
IC50: 1.8 ± 0.3–24.6 ± 4.2 mg/
mL) and normal lung MRC-5 fibroblasts (
IC50: 17.0 ± 4.2–42.1 ± 6.1 mg/mL) showed that EN, and especially AE possess
selective anticancer activity (SI values 3.41 and 9.44, respectively). Slight genotoxicity was observed only for AE and EX,
indicating the low risk concerning this feature. Notable antioxidative and anticandidal activities, selective cytotoxicity against
colorectal carcinoma cells, and absence/low genotoxicity pointed out that ALF—cultivated P. flabellatus mycelium could
be considered as a valuable source of bioactive substances.
PB  - Springer Science and Business Media B.V.
T2  - World Journal of Microbiology and Biotechnology
T1  - Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities
IS  - 1
SP  - 17
VL  - 37
DO  - 10.1007/s11274-020-02980-6
ER  - 

@article{
author = "Klaus, Anita and Wan‑Mohtar, Wan Abd Al Qadr Imad and Nikolić, Biljana and Cvetković, Stefana and Vunduk, Jovana",
year = "2021",
abstract = "Four types of mycelial extracts were derived from the airlift liquid fermentation (ALF) of Pleurotus flabellatus, namely
exopolysaccharide (EX), endopolysaccharide (EN), hot water (WE), and hot alkali (AE) extracts. Such extracts were screened
for their active components and biological potential. EN proved to be most effective in inhibition of lipid peroxidation
(
EC50 = 1.71 ± 0.02 mg/mL) and in Cupric ion reducing antioxidant capacity (CUPRAC) assay (
EC50 = 2.91 ± 0.01 mg
TE/g). AE exhibited most pronounced ability to chelate ferrous ions (
EC50 = 4.96 ± 0.08 mg/mL) and to scavenge ABTS
radicals (
EC50 = 3.36 ± 0.03 mg TE/g). β-glucans and total phenols contributed most to the chelating ability and quenching
of ABTS radicals. Inhibition of lipid peroxidation correlated best with total glucans, total proteins, and β-glucans. Total
proteins contributed most to CUPRAC antioxidant capacity. Antifungal effect was determined against Candida albicans
ATCC 10231 (MIC: 0.019–0.625 mg/mL; MFC: 0.039–2.5 mg/mL), and towards C. albicans clinical isolate (MIC and MFC:
10.0–20.0 mg/mL). Comparison of cytotoxicity against colorectal carcinoma HCT 116 cells (
IC50: 1.8 ± 0.3–24.6 ± 4.2 mg/
mL) and normal lung MRC-5 fibroblasts (
IC50: 17.0 ± 4.2–42.1 ± 6.1 mg/mL) showed that EN, and especially AE possess
selective anticancer activity (SI values 3.41 and 9.44, respectively). Slight genotoxicity was observed only for AE and EX,
indicating the low risk concerning this feature. Notable antioxidative and anticandidal activities, selective cytotoxicity against
colorectal carcinoma cells, and absence/low genotoxicity pointed out that ALF—cultivated P. flabellatus mycelium could
be considered as a valuable source of bioactive substances.",
publisher = "Springer Science and Business Media B.V.",
journal = "World Journal of Microbiology and Biotechnology",
title = "Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities",
number = "1",
pages = "17",
volume = "37",
doi = "10.1007/s11274-020-02980-6"
}

Klaus, A., Wan‑Mohtar, W. A. A. Q. I., Nikolić, B., Cvetković, S.,& Vunduk, J.. (2021). Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities. in World Journal of Microbiology and Biotechnology
Springer Science and Business Media B.V.., 37(1), 17.
https://doi.org/10.1007/s11274-020-02980-6

Klaus A, Wan‑Mohtar WAAQI, Nikolić B, Cvetković S, Vunduk J. Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities. in World Journal of Microbiology and Biotechnology. 2021;37(1):17.
doi:10.1007/s11274-020-02980-6 .

Klaus, Anita, Wan‑Mohtar, Wan Abd Al Qadr Imad, Nikolić, Biljana, Cvetković, Stefana, Vunduk, Jovana, "Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities" in World Journal of Microbiology and Biotechnology, 37, no. 1 (2021):17,
https://doi.org/10.1007/s11274-020-02980-6 . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Nikšić, Miomir
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5083
AB  - Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin's barrier function, and provide a skin-lightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction (HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50 = 1.65 +/- 0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes
EP  - 264
IS  - 2
SP  - 253
VL  - 71
DO  - 10.2298/ABS181217007K
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Wan-Mohtar, Wan Abd Al Qadr Imad and Nikšić, Miomir",
year = "2019",
abstract = "Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin's barrier function, and provide a skin-lightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction (HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50 = 1.65 +/- 0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes",
pages = "264-253",
number = "2",
volume = "71",
doi = "10.2298/ABS181217007K"
}

Kozarski, M., Klaus, A., Jakovljević, D., Todorović, N., Wan-Mohtar, W. A. A. Q. I.,& Nikšić, M.. (2019). Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(2), 253-264.
https://doi.org/10.2298/ABS181217007K

Kozarski M, Klaus A, Jakovljević D, Todorović N, Wan-Mohtar WAAQI, Nikšić M. Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences. 2019;71(2):253-264.
doi:10.2298/ABS181217007K .

Kozarski, Maja, Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, Nikšić, Miomir, "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes" in Archives of Biological Sciences, 71, no. 2 (2019):253-264,
https://doi.org/10.2298/ABS181217007K . .

TY  - JOUR
AU  - Vunduk, Jovana
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Mohamad, Shaiful Azuar
AU  - Abd Halim, Nur Hafizati
AU  - Dzomir, Ahmad Zainuri Mohd
AU  - Zizak, Zeljko
AU  - Klaus, Anita
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4940
AB  - Foodborne bacteria biofilms present a major concern for the food industry. Although their numerous biological activities are well established, there is little research to date on the use of polysaccharides of mushroom origin as a possible solution for preventing biofilm formation. Therefore, the aim of this study was to examine the anti adhesion and antibiofilm effects of several types of Pleurotus flabellatus strain Mynuk polysaccharide extracts (PFSMpe), produced by air-lift submerged fermentation, against American Type Culture Collection (ATCC) and clinical strains of foodborne bacteria, as well as the cytotoxicity of these extracts. PFSMpe exhibited antiadhesion activity toward clinical isolates, and the percentage of adhesion inhibition was highest for water (WE) and exopolysaccharide (EXOPE) extracts (> 50%) against Enterococcus faecalis. Selected ATCC strains were more resistant than clinical strains, indicating the antiquorum sensing mechanism of PFSMpe action. Antibiofilm activity was similar to antiadhesion activity and WE showed the strongest effect, again on E. faecalis. Differences in antiadhesion and antibiofilm effects of PFSMpe may be explained by differences in chemical composition, with crude extracts showing greater efficiency due to a synergistic effect. PFSMpe did not exhibit cyctotoxic activity against normal human cell lines. Overall, the findings of this study show that PFSMpe represents a promising novel strategy against bacterial biofilms.
PB  - Elsevier, Amsterdam
T2  - LWT-Food Science and Technology
T1  - Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens
VL  - 112
DO  - 10.1016/j.lwt.2019.05.119
ER  - 

@article{
author = "Vunduk, Jovana and Wan-Mohtar, Wan Abd Al Qadr Imad and Mohamad, Shaiful Azuar and Abd Halim, Nur Hafizati and Dzomir, Ahmad Zainuri Mohd and Zizak, Zeljko and Klaus, Anita",
year = "2019",
abstract = "Foodborne bacteria biofilms present a major concern for the food industry. Although their numerous biological activities are well established, there is little research to date on the use of polysaccharides of mushroom origin as a possible solution for preventing biofilm formation. Therefore, the aim of this study was to examine the anti adhesion and antibiofilm effects of several types of Pleurotus flabellatus strain Mynuk polysaccharide extracts (PFSMpe), produced by air-lift submerged fermentation, against American Type Culture Collection (ATCC) and clinical strains of foodborne bacteria, as well as the cytotoxicity of these extracts. PFSMpe exhibited antiadhesion activity toward clinical isolates, and the percentage of adhesion inhibition was highest for water (WE) and exopolysaccharide (EXOPE) extracts (> 50%) against Enterococcus faecalis. Selected ATCC strains were more resistant than clinical strains, indicating the antiquorum sensing mechanism of PFSMpe action. Antibiofilm activity was similar to antiadhesion activity and WE showed the strongest effect, again on E. faecalis. Differences in antiadhesion and antibiofilm effects of PFSMpe may be explained by differences in chemical composition, with crude extracts showing greater efficiency due to a synergistic effect. PFSMpe did not exhibit cyctotoxic activity against normal human cell lines. Overall, the findings of this study show that PFSMpe represents a promising novel strategy against bacterial biofilms.",
publisher = "Elsevier, Amsterdam",
journal = "LWT-Food Science and Technology",
title = "Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens",
volume = "112",
doi = "10.1016/j.lwt.2019.05.119"
}

Vunduk, J., Wan-Mohtar, W. A. A. Q. I., Mohamad, S. A., Abd Halim, N. H., Dzomir, A. Z. M., Zizak, Z.,& Klaus, A.. (2019). Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens. in LWT-Food Science and Technology
Elsevier, Amsterdam., 112.
https://doi.org/10.1016/j.lwt.2019.05.119

Vunduk J, Wan-Mohtar WAAQI, Mohamad SA, Abd Halim NH, Dzomir AZM, Zizak Z, Klaus A. Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens. in LWT-Food Science and Technology. 2019;112.
doi:10.1016/j.lwt.2019.05.119 .

Vunduk, Jovana, Wan-Mohtar, Wan Abd Al Qadr Imad, Mohamad, Shaiful Azuar, Abd Halim, Nur Hafizati, Dzomir, Ahmad Zainuri Mohd, Zizak, Zeljko, Klaus, Anita, "Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens" in LWT-Food Science and Technology, 112 (2019),
https://doi.org/10.1016/j.lwt.2019.05.119 . .

TY  - JOUR
AU  - Hassan, Nurul Amirah
AU  - Supramani, Sugenendran
AU  - Sohedein, Mohamad Nor Azzimi
AU  - Usuldin, Siti Rokhiyah Ahmad
AU  - Klaus, Anita
AU  - Ilham, Zul
AU  - Chen, Wei-Hsin
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5007
AB  - A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p  lt  0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.
PB  - Elsevier, Amsterdam
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation
VL  - 21
DO  - 10.1016/j.bcab.2019.101305
ER  - 

@article{
author = "Hassan, Nurul Amirah and Supramani, Sugenendran and Sohedein, Mohamad Nor Azzimi and Usuldin, Siti Rokhiyah Ahmad and Klaus, Anita and Ilham, Zul and Chen, Wei-Hsin and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p  lt  0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.",
publisher = "Elsevier, Amsterdam",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation",
volume = "21",
doi = "10.1016/j.bcab.2019.101305"
}

Hassan, N. A., Supramani, S., Sohedein, M. N. A., Usuldin, S. R. A., Klaus, A., Ilham, Z., Chen, W.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology
Elsevier, Amsterdam., 21.
https://doi.org/10.1016/j.bcab.2019.101305

Hassan NA, Supramani S, Sohedein MNA, Usuldin SRA, Klaus A, Ilham Z, Chen W, Wan-Mohtar WAAQI. Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology. 2019;21.
doi:10.1016/j.bcab.2019.101305 .

Hassan, Nurul Amirah, Supramani, Sugenendran, Sohedein, Mohamad Nor Azzimi, Usuldin, Siti Rokhiyah Ahmad, Klaus, Anita, Ilham, Zul, Chen, Wei-Hsin, Wan-Mohtar, Wan Abd Al Qadr Imad, "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation" in Biocatalysis and Agricultural Biotechnology, 21 (2019),
https://doi.org/10.1016/j.bcab.2019.101305 . .

TY  - JOUR
AU  - Supramani, Sugenendran
AU  - Ahmad, Rahayu
AU  - Ilham, Zul
AU  - Annuar, Mohamad Suffian Mohamad
AU  - Klaus, Anita
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5018
AB  - Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p  lt  0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p  lt  0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.
PB  - Amer Inst Mathematical Sciences-Aims, Springfield
T2  - Aims Microbiology
T1  - Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology
EP  - 38
IS  - 1
SP  - 19
VL  - 5
DO  - 10.3934/microbiol.2019.1.19
ER  - 

@article{
author = "Supramani, Sugenendran and Ahmad, Rahayu and Ilham, Zul and Annuar, Mohamad Suffian Mohamad and Klaus, Anita and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p  lt  0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p  lt  0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.",
publisher = "Amer Inst Mathematical Sciences-Aims, Springfield",
journal = "Aims Microbiology",
title = "Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology",
pages = "38-19",
number = "1",
volume = "5",
doi = "10.3934/microbiol.2019.1.19"
}

Supramani, S., Ahmad, R., Ilham, Z., Annuar, M. S. M., Klaus, A.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology. in Aims Microbiology
Amer Inst Mathematical Sciences-Aims, Springfield., 5(1), 19-38.
https://doi.org/10.3934/microbiol.2019.1.19

Supramani S, Ahmad R, Ilham Z, Annuar MSM, Klaus A, Wan-Mohtar WAAQI. Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology. in Aims Microbiology. 2019;5(1):19-38.
doi:10.3934/microbiol.2019.1.19 .

Supramani, Sugenendran, Ahmad, Rahayu, Ilham, Zul, Annuar, Mohamad Suffian Mohamad, Klaus, Anita, Wan-Mohtar, Wan Abd Al Qadr Imad, "Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology" in Aims Microbiology, 5, no. 1 (2019):19-38,
https://doi.org/10.3934/microbiol.2019.1.19 . .

TY  - JOUR
AU  - Supramani, Sugenendran
AU  - Jailani, Nurulhuda
AU  - Ramarao, Kivaandra
AU  - Zain, Nurul Amalina Mohd
AU  - Klaus, Anita
AU  - Ahmad, Rahayu
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4959
AB  - The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 degrees C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day(-1)) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 mu m  lt  d  lt  40.67 mu m) were associated with high DCW while small dispersed pellets (diameter: 11.67 mu m  lt  d  lt  14.67 mu m) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 mu m  lt  d  lt  7.67 mu m) to RBF4 (16.67 mu m  lt  d  lt  18.33 mu m). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day(-1) to 0.108 g/L day(-1). In an FTIR analysis, crude EPS of EGP showed the presence of beta-glucan characteristics at 1075 cm(-1) and 891 cm(-1) wavelengths, similar with European G. lucidum BCCM 31549. In addition, H-1 NMR showed similar beta-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production.
PB  - Elsevier, Amsterdam
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production
VL  - 19
DO  - 10.1016/j.bcab.2019.101118
ER  - 

@article{
author = "Supramani, Sugenendran and Jailani, Nurulhuda and Ramarao, Kivaandra and Zain, Nurul Amalina Mohd and Klaus, Anita and Ahmad, Rahayu and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "The pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 degrees C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day(-1)) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 mu m  lt  d  lt  40.67 mu m) were associated with high DCW while small dispersed pellets (diameter: 11.67 mu m  lt  d  lt  14.67 mu m) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 mu m  lt  d  lt  7.67 mu m) to RBF4 (16.67 mu m  lt  d  lt  18.33 mu m). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day(-1) to 0.108 g/L day(-1). In an FTIR analysis, crude EPS of EGP showed the presence of beta-glucan characteristics at 1075 cm(-1) and 891 cm(-1) wavelengths, similar with European G. lucidum BCCM 31549. In addition, H-1 NMR showed similar beta-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production.",
publisher = "Elsevier, Amsterdam",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production",
volume = "19",
doi = "10.1016/j.bcab.2019.101118"
}

Supramani, S., Jailani, N., Ramarao, K., Zain, N. A. M., Klaus, A., Ahmad, R.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production. in Biocatalysis and Agricultural Biotechnology
Elsevier, Amsterdam., 19.
https://doi.org/10.1016/j.bcab.2019.101118

Supramani S, Jailani N, Ramarao K, Zain NAM, Klaus A, Ahmad R, Wan-Mohtar WAAQI. Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production. in Biocatalysis and Agricultural Biotechnology. 2019;19.
doi:10.1016/j.bcab.2019.101118 .

Supramani, Sugenendran, Jailani, Nurulhuda, Ramarao, Kivaandra, Zain, Nurul Amalina Mohd, Klaus, Anita, Ahmad, Rahayu, Wan-Mohtar, Wan Abd Al Qadr Imad, "Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production" in Biocatalysis and Agricultural Biotechnology, 19 (2019),
https://doi.org/10.1016/j.bcab.2019.101118 . .

TY  - JOUR
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Klaus, Anita
AU  - Cheng, Acga
AU  - Salis, Shardana Aiga
AU  - Halim-Lim, Sarina Abdul
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4933
AB  - Purpose The purpose of this paper is to identify the strain of oyster mushroom (OM) Pleurotus sapidus cultivated in a local (commercial) farm, and to generate a total quality index (TQI) on the strain using different modified atmosphere packaging (MAP) gas mixtures. Design/methodology/approach A phylogenetic tree was constructed using the MEGA software to identify the specific strain of P. sapidus grown in a local farm. The effects of MAP on fresh fruiting bodies of the identified strain were determined under three conditions: high carbon dioxide packaging (HCP: 20 per cent CO2, 15 per cent O-2), low carbon dioxide packaging (LCP: 2 per cent CO2, 30 per cent O-2) and high nitrogen packaging (HNP: 85 per cent N-2, 15 per cent O-2). All samples were stored at 4 C-o for up to ten days, and subjected to total phenolic content (TPC), colour retention (CR) and sensory analysis. Quality parameters such as chewiness and odour were used to obtain the TQI. Findings From the phylogenetic analysis, a new strain (P. sapidus strain QDR) with 99 per cent similarity to P. sapidus was identified. Among the three MAP treatments, HCP recorded the highest TPC (2.85 mg GAE/g) and CR (60.36) after ten days, although only its CR was significantly different (p lt 0.05) from the control. Feedback from 30 sensory panellists indicated that both HCP and LCP were generally more effective in retaining the colour-odour of OM. The optimum TQI for HCP was obtained based on the observed parameters, which is useful for the large-scale packaging of OM. Originality/value Scientific evidence has revealed that packaging trend for commercially grown OM affects consumer's acceptance.
PB  - Emerald Group Publishing Ltd, Bingley
T2  - British Food Journal
T1  - Total quality index of commercial oyster mushroom Pleurotus sapidus in modified atmosphere packaging
EP  - 1883
IS  - 8
SP  - 1871
VL  - 121
DO  - 10.1108/BFJ-06-2018-0408
ER  - 

@article{
author = "Wan-Mohtar, Wan Abd Al Qadr Imad and Klaus, Anita and Cheng, Acga and Salis, Shardana Aiga and Halim-Lim, Sarina Abdul",
year = "2019",
abstract = "Purpose The purpose of this paper is to identify the strain of oyster mushroom (OM) Pleurotus sapidus cultivated in a local (commercial) farm, and to generate a total quality index (TQI) on the strain using different modified atmosphere packaging (MAP) gas mixtures. Design/methodology/approach A phylogenetic tree was constructed using the MEGA software to identify the specific strain of P. sapidus grown in a local farm. The effects of MAP on fresh fruiting bodies of the identified strain were determined under three conditions: high carbon dioxide packaging (HCP: 20 per cent CO2, 15 per cent O-2), low carbon dioxide packaging (LCP: 2 per cent CO2, 30 per cent O-2) and high nitrogen packaging (HNP: 85 per cent N-2, 15 per cent O-2). All samples were stored at 4 C-o for up to ten days, and subjected to total phenolic content (TPC), colour retention (CR) and sensory analysis. Quality parameters such as chewiness and odour were used to obtain the TQI. Findings From the phylogenetic analysis, a new strain (P. sapidus strain QDR) with 99 per cent similarity to P. sapidus was identified. Among the three MAP treatments, HCP recorded the highest TPC (2.85 mg GAE/g) and CR (60.36) after ten days, although only its CR was significantly different (p lt 0.05) from the control. Feedback from 30 sensory panellists indicated that both HCP and LCP were generally more effective in retaining the colour-odour of OM. The optimum TQI for HCP was obtained based on the observed parameters, which is useful for the large-scale packaging of OM. Originality/value Scientific evidence has revealed that packaging trend for commercially grown OM affects consumer's acceptance.",
publisher = "Emerald Group Publishing Ltd, Bingley",
journal = "British Food Journal",
title = "Total quality index of commercial oyster mushroom Pleurotus sapidus in modified atmosphere packaging",
pages = "1883-1871",
number = "8",
volume = "121",
doi = "10.1108/BFJ-06-2018-0408"
}

Wan-Mohtar, W. A. A. Q. I., Klaus, A., Cheng, A., Salis, S. A.,& Halim-Lim, S. A.. (2019). Total quality index of commercial oyster mushroom Pleurotus sapidus in modified atmosphere packaging. in British Food Journal
Emerald Group Publishing Ltd, Bingley., 121(8), 1871-1883.
https://doi.org/10.1108/BFJ-06-2018-0408

Wan-Mohtar WAAQI, Klaus A, Cheng A, Salis SA, Halim-Lim SA. Total quality index of commercial oyster mushroom Pleurotus sapidus in modified atmosphere packaging. in British Food Journal. 2019;121(8):1871-1883.
doi:10.1108/BFJ-06-2018-0408 .

Wan-Mohtar, Wan Abd Al Qadr Imad, Klaus, Anita, Cheng, Acga, Salis, Shardana Aiga, Halim-Lim, Sarina Abdul, "Total quality index of commercial oyster mushroom Pleurotus sapidus in modified atmosphere packaging" in British Food Journal, 121, no. 8 (2019):1871-1883,
https://doi.org/10.1108/BFJ-06-2018-0408 . .

TY  - JOUR
AU  - Hanafiah, Zarimah Mohd
AU  - Mohtar, Wan Hanna Melini Wan
AU  - Abu Hasan, Hassimi
AU  - Jensen, Henriette Stokbro
AU  - Klaus, Anita
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4956
AB  - The fluctuation of domestic wastewater characteristic inhibits the current conventional microbial-based treatment. The bioremediation fungi has received attention and reported to be an effective alternative to treat industrial wastewater. Similar efficient performance is envisaged for domestic wastewater whereby assessed performance of fungi for varying carbon-to-nitrogen ratios in domestic wastewater is crucial. Thus, the performance of pre-grown wild-Serbian Ganoderma lucidum mycelial pellets (GLMPs) was evaluated on four different synthetic domestic wastewaters under different conditions of initial pH (pH 4, 5, and 7) and chemical oxygen demand (COD) to nitrogen (COD/N) ratio of 3.6:1, 7.1:1, 14.2:1, and 17.8:1 (C3.6N1, C7.1N1, C14.2N1, and C17.8N1). The COD/N ratios with a constant concentration of ammonia-nitrogen (NH3-N) were chosen on the basis of the urban domestic wastewater characteristics sampled at the inlet basin of a sewage treatment plant (STP). The parameters of pH, COD, and NH3-N were measured periodically during the experiment. The wild-Serbian GLMPs efficiently removed the pollutants from the synthetic sewage. The COD/N ratio of C17.8N1 wastewater had the best COD and NH3-N removal, as compared to the lower COD/N ratio, and the shortest treatment time was obtained in an acidic environment at pH 4. The highest percentage for COD and NH3-N removal achieved was 96.0% and 93.2%, respectively. The results proved that the mycelium of GLMP has high potential in treating domestic wastewater, particularly at high organic content as a naturally sustainable bioremediation system.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor
VL  - 9
DO  - 10.1038/s41598-019-52493-y
ER  - 

@article{
author = "Hanafiah, Zarimah Mohd and Mohtar, Wan Hanna Melini Wan and Abu Hasan, Hassimi and Jensen, Henriette Stokbro and Klaus, Anita and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "The fluctuation of domestic wastewater characteristic inhibits the current conventional microbial-based treatment. The bioremediation fungi has received attention and reported to be an effective alternative to treat industrial wastewater. Similar efficient performance is envisaged for domestic wastewater whereby assessed performance of fungi for varying carbon-to-nitrogen ratios in domestic wastewater is crucial. Thus, the performance of pre-grown wild-Serbian Ganoderma lucidum mycelial pellets (GLMPs) was evaluated on four different synthetic domestic wastewaters under different conditions of initial pH (pH 4, 5, and 7) and chemical oxygen demand (COD) to nitrogen (COD/N) ratio of 3.6:1, 7.1:1, 14.2:1, and 17.8:1 (C3.6N1, C7.1N1, C14.2N1, and C17.8N1). The COD/N ratios with a constant concentration of ammonia-nitrogen (NH3-N) were chosen on the basis of the urban domestic wastewater characteristics sampled at the inlet basin of a sewage treatment plant (STP). The parameters of pH, COD, and NH3-N were measured periodically during the experiment. The wild-Serbian GLMPs efficiently removed the pollutants from the synthetic sewage. The COD/N ratio of C17.8N1 wastewater had the best COD and NH3-N removal, as compared to the lower COD/N ratio, and the shortest treatment time was obtained in an acidic environment at pH 4. The highest percentage for COD and NH3-N removal achieved was 96.0% and 93.2%, respectively. The results proved that the mycelium of GLMP has high potential in treating domestic wastewater, particularly at high organic content as a naturally sustainable bioremediation system.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor",
volume = "9",
doi = "10.1038/s41598-019-52493-y"
}

Hanafiah, Z. M., Mohtar, W. H. M. W., Abu Hasan, H., Jensen, H. S., Klaus, A.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor. in Scientific Reports
Nature Publishing Group, London., 9.
https://doi.org/10.1038/s41598-019-52493-y

Hanafiah ZM, Mohtar WHMW, Abu Hasan H, Jensen HS, Klaus A, Wan-Mohtar WAAQI. Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor. in Scientific Reports. 2019;9.
doi:10.1038/s41598-019-52493-y .

Hanafiah, Zarimah Mohd, Mohtar, Wan Hanna Melini Wan, Abu Hasan, Hassimi, Jensen, Henriette Stokbro, Klaus, Anita, Wan-Mohtar, Wan Abd Al Qadr Imad, "Performance of wild-Serbian Ganoderma lucidum mycelium in treating synthetic sewage loading using batch bioreactor" in Scientific Reports, 9 (2019),
https://doi.org/10.1038/s41598-019-52493-y . .

TY  - JOUR
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Viegelmann, Christina
AU  - Klaus, Anita
AU  - Lim, Sarina Abdul Halim
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4379
AB  - Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 mu g/mL to produce 0.45 mu M of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.
PB  - Korean Society Food Science & Technology-Kosfost, Seoul
T2  - Food Science and Biotechnology
T1  - Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum
EP  - 165
IS  - 1
SP  - 159
VL  - 26
DO  - 10.1007/s10068-017-0021-6
ER  - 

@article{
author = "Wan-Mohtar, Wan Abd Al Qadr Imad and Viegelmann, Christina and Klaus, Anita and Lim, Sarina Abdul Halim",
year = "2017",
abstract = "Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 mu g/mL to produce 0.45 mu M of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.",
publisher = "Korean Society Food Science & Technology-Kosfost, Seoul",
journal = "Food Science and Biotechnology",
title = "Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum",
pages = "165-159",
number = "1",
volume = "26",
doi = "10.1007/s10068-017-0021-6"
}

Wan-Mohtar, W. A. A. Q. I., Viegelmann, C., Klaus, A.,& Lim, S. A. H.. (2017). Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum. in Food Science and Biotechnology
Korean Society Food Science & Technology-Kosfost, Seoul., 26(1), 159-165.
https://doi.org/10.1007/s10068-017-0021-6

Wan-Mohtar WAAQI, Viegelmann C, Klaus A, Lim SAH. Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum. in Food Science and Biotechnology. 2017;26(1):159-165.
doi:10.1007/s10068-017-0021-6 .

Wan-Mohtar, Wan Abd Al Qadr Imad, Viegelmann, Christina, Klaus, Anita, Lim, Sarina Abdul Halim, "Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum" in Food Science and Biotechnology, 26, no. 1 (2017):159-165,
https://doi.org/10.1007/s10068-017-0021-6 . .