TY  - JOUR
AU  - Miletić, Dunja
AU  - Turło, Jadwiga
AU  - Podsadni, Piotr
AU  - Sknepnek, Aleksandra
AU  - Szczepanska, Agnieszka
AU  - Klimaszewska, Marzenna
AU  - Malinowska, Eliza
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5836
AB  - During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor
SP  - 101046
VL  - 42
DO  - 10.1016/j.fbio.2021.101046
ER  - 

@article{
author = "Miletić, Dunja and Turło, Jadwiga and Podsadni, Piotr and Sknepnek, Aleksandra and Szczepanska, Agnieszka and Klimaszewska, Marzenna and Malinowska, Eliza and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
abstract = "During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor",
pages = "101046",
volume = "42",
doi = "10.1016/j.fbio.2021.101046"
}

Miletić, D., Turło, J., Podsadni, P., Sknepnek, A., Szczepanska, A., Klimaszewska, M., Malinowska, E., Lević, S., Nedović, V.,& Nikšić, M.. (2021). Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience
Elsevier Ltd., 42, 101046.
https://doi.org/10.1016/j.fbio.2021.101046

Miletić D, Turło J, Podsadni P, Sknepnek A, Szczepanska A, Klimaszewska M, Malinowska E, Lević S, Nedović V, Nikšić M. Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience. 2021;42:101046.
doi:10.1016/j.fbio.2021.101046 .

Miletić, Dunja, Turło, Jadwiga, Podsadni, Piotr, Sknepnek, Aleksandra, Szczepanska, Agnieszka, Klimaszewska, Marzenna, Malinowska, Eliza, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor" in Food Bioscience, 42 (2021):101046,
https://doi.org/10.1016/j.fbio.2021.101046 . .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Turlo, Jadwiga
AU  - Podsadni, Piotr
AU  - Sknepnek, Aleksandra
AU  - Szczepanska, Agnieszka
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5287
AB  - Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing. Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower. However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order reducing power > DPPH scavenging ability > chelating ability. Due to the high carbohydrate and beta-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities. D-glucose was the main monosaccharide (87.18 +/- 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate. In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity
EP  - 895
IS  - 9
SP  - 885
VL  - 22
DO  - 10.1615/IntJMedMushrooms.2020035877
ER  - 

@article{
author = "Miletić, Dunja and Turlo, Jadwiga and Podsadni, Piotr and Sknepnek, Aleksandra and Szczepanska, Agnieszka and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2020",
abstract = "Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing. Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower. However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order reducing power > DPPH scavenging ability > chelating ability. Due to the high carbohydrate and beta-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities. D-glucose was the main monosaccharide (87.18 +/- 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate. In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity",
pages = "895-885",
number = "9",
volume = "22",
doi = "10.1615/IntJMedMushrooms.2020035877"
}

Miletić, D., Turlo, J., Podsadni, P., Sknepnek, A., Szczepanska, A., Lević, S., Nedović, V.,& Nikšić, M.. (2020). Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 22(9), 885-895.
https://doi.org/10.1615/IntJMedMushrooms.2020035877

Miletić D, Turlo J, Podsadni P, Sknepnek A, Szczepanska A, Lević S, Nedović V, Nikšić M. Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity. in International Journal of Medicinal Mushrooms. 2020;22(9):885-895.
doi:10.1615/IntJMedMushrooms.2020035877 .

Miletić, Dunja, Turlo, Jadwiga, Podsadni, Piotr, Sknepnek, Aleksandra, Szczepanska, Agnieszka, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity" in International Journal of Medicinal Mushrooms, 22, no. 9 (2020):885-895,
https://doi.org/10.1615/IntJMedMushrooms.2020035877 . .

TY  - CONF
AU  - Savić, Milena
AU  - Podsadni, Piotr
AU  - Bamburowicz-Klimkowska, Magdalena
AU  - Klimaszewska, Marzenna
AU  - Szutowski, Miroslaw
AU  - Nedovic, Viktor
AU  - Jadwiga, Turlo
AU  - Nikšić, Miomir
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6618
AB  - The aim of this study was to investigate the potential influence of selenized yeast (Sel Plex, Alltech Inc., Lexington, USA) on chemical composition and flavour of solid state grown mushroom Pleurotus ostreatus. Amino acid composition that influences the flavour of selenium-enriched P.ostreatus P80 (137.84 ppm of selenium in d.w.) and non enriched cultivated strains with particular emphasis on selenomethionine was determined by HPLC method, after complete hydrolysis. Volatile flavour compounds of mushroom cultures were analyzed by GC-MS using Headspace sampler. In mushrooms with high selenium content, selenium in the form of L-selenomethionine was present. High selenium concentration in fruit body did not significantly change the amino acid composition of mushrooms. The major amino acids of fruit body sample were glutamic acid, alanine, aspartic acid and tryptophan. In the fruit body of P.ostreatus P80 control, 30 volatile compounds were detected, and in selenium enriched sample number of detected compounds was 25. Compounds that were detected in control sample, but not in eriched fruit body are 2-amino-N-ethylpropanamide, 2-methylpropyl pentan-2-yl sulfite, 2,3-pentanedione, heptan-2-one, pentan-1-ol, 2-methyldihydrofuran-3(2H)-one,1-hydroxyacetone, 3-hydroxy-2-butanon, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine and benzaldehyde. Compounds that were detected in enriched P.ostreatus P80, but not in control are acetone, ethylacetate, 2-propanol, acetonitrile, 2-methylbenzaldehyde and 2-hydroxyethylmethacrylate. Selenized yeast presents good source of selenium for selenium enriched mushroom production with good flavour, which is probably safety for consumption.
PB  - University of Novi Sad Institute of Food Technology
C3  - II International Congress “Food Technology, Quality and Safety”
T1  - Selenized yeast in production of selenium enriched Pleurotus ostreatus mushroom with good flavour
EP  - 520
SP  - 516
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6618
ER  - 

@conference{
author = "Savić, Milena and Podsadni, Piotr and Bamburowicz-Klimkowska, Magdalena and Klimaszewska, Marzenna and Szutowski, Miroslaw and Nedovic, Viktor and Jadwiga, Turlo and Nikšić, Miomir",
year = "2014",
abstract = "The aim of this study was to investigate the potential influence of selenized yeast (Sel Plex, Alltech Inc., Lexington, USA) on chemical composition and flavour of solid state grown mushroom Pleurotus ostreatus. Amino acid composition that influences the flavour of selenium-enriched P.ostreatus P80 (137.84 ppm of selenium in d.w.) and non enriched cultivated strains with particular emphasis on selenomethionine was determined by HPLC method, after complete hydrolysis. Volatile flavour compounds of mushroom cultures were analyzed by GC-MS using Headspace sampler. In mushrooms with high selenium content, selenium in the form of L-selenomethionine was present. High selenium concentration in fruit body did not significantly change the amino acid composition of mushrooms. The major amino acids of fruit body sample were glutamic acid, alanine, aspartic acid and tryptophan. In the fruit body of P.ostreatus P80 control, 30 volatile compounds were detected, and in selenium enriched sample number of detected compounds was 25. Compounds that were detected in control sample, but not in eriched fruit body are 2-amino-N-ethylpropanamide, 2-methylpropyl pentan-2-yl sulfite, 2,3-pentanedione, heptan-2-one, pentan-1-ol, 2-methyldihydrofuran-3(2H)-one,1-hydroxyacetone, 3-hydroxy-2-butanon, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine and benzaldehyde. Compounds that were detected in enriched P.ostreatus P80, but not in control are acetone, ethylacetate, 2-propanol, acetonitrile, 2-methylbenzaldehyde and 2-hydroxyethylmethacrylate. Selenized yeast presents good source of selenium for selenium enriched mushroom production with good flavour, which is probably safety for consumption.",
publisher = "University of Novi Sad Institute of Food Technology",
journal = "II International Congress “Food Technology, Quality and Safety”",
title = "Selenized yeast in production of selenium enriched Pleurotus ostreatus mushroom with good flavour",
pages = "520-516",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6618"
}

Savić, M., Podsadni, P., Bamburowicz-Klimkowska, M., Klimaszewska, M., Szutowski, M., Nedovic, V., Jadwiga, T.,& Nikšić, M.. (2014). Selenized yeast in production of selenium enriched Pleurotus ostreatus mushroom with good flavour. in II International Congress “Food Technology, Quality and Safety”
University of Novi Sad Institute of Food Technology., 516-520.
https://hdl.handle.net/21.15107/rcub_agrospace_6618

Savić M, Podsadni P, Bamburowicz-Klimkowska M, Klimaszewska M, Szutowski M, Nedovic V, Jadwiga T, Nikšić M. Selenized yeast in production of selenium enriched Pleurotus ostreatus mushroom with good flavour. in II International Congress “Food Technology, Quality and Safety”. 2014;:516-520.
https://hdl.handle.net/21.15107/rcub_agrospace_6618 .

Savić, Milena, Podsadni, Piotr, Bamburowicz-Klimkowska, Magdalena, Klimaszewska, Marzenna, Szutowski, Miroslaw, Nedovic, Viktor, Jadwiga, Turlo, Nikšić, Miomir, "Selenized yeast in production of selenium enriched Pleurotus ostreatus mushroom with good flavour" in II International Congress “Food Technology, Quality and Safety” (2014):516-520,
https://hdl.handle.net/21.15107/rcub_agrospace_6618 .