TY  - JOUR
AU  - Miletić, Dunja
AU  - Turło, Jadwiga
AU  - Podsadni, Piotr
AU  - Sknepnek, Aleksandra
AU  - Szczepanska, Agnieszka
AU  - Klimaszewska, Marzenna
AU  - Malinowska, Eliza
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5836
AB  - During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor
SP  - 101046
VL  - 42
DO  - 10.1016/j.fbio.2021.101046
ER  - 

@article{
author = "Miletić, Dunja and Turło, Jadwiga and Podsadni, Piotr and Sknepnek, Aleksandra and Szczepanska, Agnieszka and Klimaszewska, Marzenna and Malinowska, Eliza and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
abstract = "During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor",
pages = "101046",
volume = "42",
doi = "10.1016/j.fbio.2021.101046"
}

Miletić, D., Turło, J., Podsadni, P., Sknepnek, A., Szczepanska, A., Klimaszewska, M., Malinowska, E., Lević, S., Nedović, V.,& Nikšić, M.. (2021). Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience
Elsevier Ltd., 42, 101046.
https://doi.org/10.1016/j.fbio.2021.101046

Miletić D, Turło J, Podsadni P, Sknepnek A, Szczepanska A, Klimaszewska M, Malinowska E, Lević S, Nedović V, Nikšić M. Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience. 2021;42:101046.
doi:10.1016/j.fbio.2021.101046 .

Miletić, Dunja, Turło, Jadwiga, Podsadni, Piotr, Sknepnek, Aleksandra, Szczepanska, Agnieszka, Klimaszewska, Marzenna, Malinowska, Eliza, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor" in Food Bioscience, 42 (2021):101046,
https://doi.org/10.1016/j.fbio.2021.101046 . .