TY  - JOUR
AU  - Sknepnek, Aleksandra
AU  - Pantić, Milena
AU  - Matijasević, Danka
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4775
AB  - Kombucha is a nonalcoholic beverage traditionally made by fermenting black tea using a combination of yeast and acetic acid bacteria (AAB) cultures. Ganoderma lucidum hot water extract (HWE) was used-to our knowledge for the first time-to prepare a novel, health-promoting kombucha product. During the 11-day fermentation, pH, total acidity, and the numbers of yeasts and AAB were monitored. It was found that sweetened G. lucidum HWE was a good medium for yeast and AAB growth. The desired acidity for the beverage was reached on the second day (3 g/L) of the fermentation process; the maximum established acidity was 22.8 +/- 0.42 g/L. Fourier transform infrared analysis revealed that the vacuum-dried beverage is a mixture of various compounds such as polysaccharides, phenols, proteins, and lipids. Total phenolic content of the liquid sample was 4.91 +/- 0.2338 mg gallic acid equivalents/g, whereas the vacuum-dried sample had a smaller amount of phenolics (2.107 +/- 0.228 mg gallic acid equivalents/g). Established half-maximal effective concentrations for DPPH scavenging activity and reducing power were 22.8 +/- 0.17 and 10.61 +/- 0.34 mg/mL, respectively. The antibacterial testing revealed that activity does not originate solely from synthesized acetic acid. The liquid G. lucidum beverage was the most effective against the tested bacteria, with the lowest minimum inhibitory concentration (0.04 mg/ml,) against Staphylococcus epidermidis and Rhodococcus equi, and a minimum bactericidal concentration (0.16 mg/mL) against Bacillus spizizenii, B. cereus, and R. equi. The vacuum-dried sample was less effective, with the lowest minimum bactericidal concentration against the Gram-positive bacteria R. equi (1.875 mg/mL) and against the Gram-negative bacteria Proteus hauseri (30 mg/mL).
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects
EP  - 258
IS  - 3
SP  - 243
VL  - 20
DO  - 10.1615/IntJMedMushrooms.2018025833
ER  - 

@article{
author = "Sknepnek, Aleksandra and Pantić, Milena and Matijasević, Danka and Miletić, Dunja and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2018",
abstract = "Kombucha is a nonalcoholic beverage traditionally made by fermenting black tea using a combination of yeast and acetic acid bacteria (AAB) cultures. Ganoderma lucidum hot water extract (HWE) was used-to our knowledge for the first time-to prepare a novel, health-promoting kombucha product. During the 11-day fermentation, pH, total acidity, and the numbers of yeasts and AAB were monitored. It was found that sweetened G. lucidum HWE was a good medium for yeast and AAB growth. The desired acidity for the beverage was reached on the second day (3 g/L) of the fermentation process; the maximum established acidity was 22.8 +/- 0.42 g/L. Fourier transform infrared analysis revealed that the vacuum-dried beverage is a mixture of various compounds such as polysaccharides, phenols, proteins, and lipids. Total phenolic content of the liquid sample was 4.91 +/- 0.2338 mg gallic acid equivalents/g, whereas the vacuum-dried sample had a smaller amount of phenolics (2.107 +/- 0.228 mg gallic acid equivalents/g). Established half-maximal effective concentrations for DPPH scavenging activity and reducing power were 22.8 +/- 0.17 and 10.61 +/- 0.34 mg/mL, respectively. The antibacterial testing revealed that activity does not originate solely from synthesized acetic acid. The liquid G. lucidum beverage was the most effective against the tested bacteria, with the lowest minimum inhibitory concentration (0.04 mg/ml,) against Staphylococcus epidermidis and Rhodococcus equi, and a minimum bactericidal concentration (0.16 mg/mL) against Bacillus spizizenii, B. cereus, and R. equi. The vacuum-dried sample was less effective, with the lowest minimum bactericidal concentration against the Gram-positive bacteria R. equi (1.875 mg/mL) and against the Gram-negative bacteria Proteus hauseri (30 mg/mL).",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects",
pages = "258-243",
number = "3",
volume = "20",
doi = "10.1615/IntJMedMushrooms.2018025833"
}

Sknepnek, A., Pantić, M., Matijasević, D., Miletić, D., Lević, S., Nedović, V.,& Nikšić, M.. (2018). Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 20(3), 243-258.
https://doi.org/10.1615/IntJMedMushrooms.2018025833

Sknepnek A, Pantić M, Matijasević D, Miletić D, Lević S, Nedović V, Nikšić M. Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects. in International Journal of Medicinal Mushrooms. 2018;20(3):243-258.
doi:10.1615/IntJMedMushrooms.2018025833 .

Sknepnek, Aleksandra, Pantić, Milena, Matijasević, Danka, Miletić, Dunja, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects" in International Journal of Medicinal Mushrooms, 20, no. 3 (2018):243-258,
https://doi.org/10.1615/IntJMedMushrooms.2018025833 . .

TY  - JOUR
AU  - Milenković, Jelena
AU  - Hrenović, Jasna
AU  - Matijasević, Danka
AU  - Nikšić, Miomir
AU  - Rajić, Nevenka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4455
AB  - Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A > Cu-NZ ae Cu-A > Zn-NZ >>> Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates
EP  - 20281
IS  - 25
SP  - 20273
VL  - 24
DO  - 10.1007/s11356-017-9643-8
ER  - 

@article{
author = "Milenković, Jelena and Hrenović, Jasna and Matijasević, Danka and Nikšić, Miomir and Rajić, Nevenka",
year = "2017",
abstract = "Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A > Cu-NZ ae Cu-A > Zn-NZ >>> Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates",
pages = "20281-20273",
number = "25",
volume = "24",
doi = "10.1007/s11356-017-9643-8"
}

Milenković, J., Hrenović, J., Matijasević, D., Nikšić, M.,& Rajić, N.. (2017). Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(25), 20273-20281.
https://doi.org/10.1007/s11356-017-9643-8

Milenković J, Hrenović J, Matijasević D, Nikšić M, Rajić N. Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. in Environmental Science and Pollution Research. 2017;24(25):20273-20281.
doi:10.1007/s11356-017-9643-8 .

Milenković, Jelena, Hrenović, Jasna, Matijasević, Danka, Nikšić, Miomir, Rajić, Nevenka, "Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates" in Environmental Science and Pollution Research, 24, no. 25 (2017):20273-20281,
https://doi.org/10.1007/s11356-017-9643-8 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Pantić, Milena
AU  - Matijasević, Danka
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4336
AB  - New bioactive and antimicrobial biomaterials were produced by alginate-mediated biomineralization with Zn-mineral phase. The synthesis procedure is simple, cost-effective and resulted in. two different Zn-mineralized alginate nanocomposites, Zn-carbonate/Zn-alginate and Zn-phosphate/Zn-alginate. The presence of Zn-mineral phase and its type, have significantly affected nanocomposite morphology, stability, total metallic loading and potential to release Zn(II) in physiological environment. Antimicrobial experiments showed that both types of Zn-mineralized nanocomposites exhibit strong antimicrobial effect against Escherichia coli, Staphylococcus aureus and Candida albicans. These results suggest that alginate biomineralization, where minerals are salts of essential metallic ions like Zn(II), represents a'good strategy for designing multifunctional biomaterials for potential biomedical applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites
EP  - 321
SP  - 313
VL  - 165
DO  - 10.1016/j.carbpol.2017.02.064
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Pantić, Milena and Matijasević, Danka and Mitrić, Miodrag and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "New bioactive and antimicrobial biomaterials were produced by alginate-mediated biomineralization with Zn-mineral phase. The synthesis procedure is simple, cost-effective and resulted in. two different Zn-mineralized alginate nanocomposites, Zn-carbonate/Zn-alginate and Zn-phosphate/Zn-alginate. The presence of Zn-mineral phase and its type, have significantly affected nanocomposite morphology, stability, total metallic loading and potential to release Zn(II) in physiological environment. Antimicrobial experiments showed that both types of Zn-mineralized nanocomposites exhibit strong antimicrobial effect against Escherichia coli, Staphylococcus aureus and Candida albicans. These results suggest that alginate biomineralization, where minerals are salts of essential metallic ions like Zn(II), represents a'good strategy for designing multifunctional biomaterials for potential biomedical applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites",
pages = "321-313",
volume = "165",
doi = "10.1016/j.carbpol.2017.02.064"
}

Malagurski, I., Lević, S., Pantić, M., Matijasević, D., Mitrić, M., Pavlović, V.,& Dimitrijević-Branković, S.. (2017). Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 165, 313-321.
https://doi.org/10.1016/j.carbpol.2017.02.064

Malagurski I, Lević S, Pantić M, Matijasević D, Mitrić M, Pavlović V, Dimitrijević-Branković S. Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites. in Carbohydrate Polymers. 2017;165:313-321.
doi:10.1016/j.carbpol.2017.02.064 .

Malagurski, Ivana, Lević, Steva, Pantić, Milena, Matijasević, Danka, Mitrić, Miodrag, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites" in Carbohydrate Polymers, 165 (2017):313-321,
https://doi.org/10.1016/j.carbpol.2017.02.064 . .

TY  - JOUR
AU  - Matijasević, Danka
AU  - Pantić, Milena
AU  - Rašković, Božidar
AU  - Pavlović, Vladimir
AU  - Miletić, Dunja
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4017
AB  - The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis
VL  - 7
DO  - 10.3389/fmicb.2016.01226
ER  - 

@article{
author = "Matijasević, Danka and Pantić, Milena and Rašković, Božidar and Pavlović, Vladimir and Miletić, Dunja and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2016",
abstract = "The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis",
volume = "7",
doi = "10.3389/fmicb.2016.01226"
}

Matijasević, D., Pantić, M., Rašković, B., Pavlović, V., Miletić, D., Sknepnek, A.,& Nikšić, M.. (2016). The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.01226

Matijasević D, Pantić M, Rašković B, Pavlović V, Miletić D, Sknepnek A, Nikšić M. The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.01226 .

Matijasević, Danka, Pantić, Milena, Rašković, Božidar, Pavlović, Vladimir, Miletić, Dunja, Sknepnek, Aleksandra, Nikšić, Miomir, "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.01226 . .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Nedović, Viktor
AU  - Lević, Steva
AU  - Matijasević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4157
AB  - Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC)  lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Innovative Food Science & Emerging Technologies
T1  - Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds
EP  - 8
SP  - 1
VL  - 34
DO  - 10.1016/j.ifset.2015.12.028
ER  - 

@article{
author = "Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Nedović, Viktor and Lević, Steva and Matijasević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2016",
abstract = "Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC)  lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Innovative Food Science & Emerging Technologies",
title = "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds",
pages = "8-1",
volume = "34",
doi = "10.1016/j.ifset.2015.12.028"
}

Miletić, D., Pantić, M., Pavlović, V., Nedović, V., Lević, S., Matijasević, D., Sknepnek, A.,& Nikšić, M.. (2016). Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies
Elsevier Sci Ltd, Oxford., 34, 1-8.
https://doi.org/10.1016/j.ifset.2015.12.028

Miletić D, Pantić M, Pavlović V, Nedović V, Lević S, Matijasević D, Sknepnek A, Nikšić M. Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies. 2016;34:1-8.
doi:10.1016/j.ifset.2015.12.028 .

Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Nedović, Viktor, Lević, Steva, Matijasević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds" in Innovative Food Science & Emerging Technologies, 34 (2016):1-8,
https://doi.org/10.1016/j.ifset.2015.12.028 . .

TY  - JOUR
AU  - Jevtić, Sanja
AU  - Arcon, Iztok
AU  - Recnik, Aleksander
AU  - Babić, Biljana M.
AU  - Mazaj, Matjai
AU  - Pavlović, Jelena
AU  - Matijasević, Danka
AU  - Nikšić, Miomir
AU  - Rajić, Nevenka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3395
AB  - Se(IV) and Se(VI) anions are the dominant species of Se existing in aqueous systems. In this study, the iron(III)-modified natural zeolitic tuff (Fe-CLI) from the Serbian deposit Zlatokop has been investigated as an adsorbent for the Se oxyanions. Fe-CLI shows adsorption activity for both Se(IV) and Se(VI) which decreases with increasing pH. The adsorption capacity of Fe-CLI is found to be higher for Se(IV) than for Se(VI). Kinetics data follow the pseudo-second-order model and the obtained parameters k indicate that the rates of adsorption and desorption are higher for Se(VI). Extended X-ray absorption fine structure (EXAFS) and X-ray absorption near edge structure (XANES) analyses reveal that Se is bound at the zeolite surface forming not only the Se-O-Fe but also Se-O-Si bonds. The adsorption mechanism depends of the type of oxo ions. Samples of zeolitic tuff which contain Se were tested as soil supplements for the cultivation of Pleurotus ostreatus mushrooms. The fungus adsorbed the inorganic Se from zeolitic tuff transforming it to a more valuable organically bound form.
PB  - Elsevier, Amsterdam
T2  - Microporous and Mesoporous Materials
T1  - The iron(III)-modified natural zeolitic tuff as an adsorbent and carrier for selenium oxyanions
EP  - 100
SP  - 92
VL  - 197
DO  - 10.1016/j.micromeso.2014.06.008
ER  - 

@article{
author = "Jevtić, Sanja and Arcon, Iztok and Recnik, Aleksander and Babić, Biljana M. and Mazaj, Matjai and Pavlović, Jelena and Matijasević, Danka and Nikšić, Miomir and Rajić, Nevenka",
year = "2014",
abstract = "Se(IV) and Se(VI) anions are the dominant species of Se existing in aqueous systems. In this study, the iron(III)-modified natural zeolitic tuff (Fe-CLI) from the Serbian deposit Zlatokop has been investigated as an adsorbent for the Se oxyanions. Fe-CLI shows adsorption activity for both Se(IV) and Se(VI) which decreases with increasing pH. The adsorption capacity of Fe-CLI is found to be higher for Se(IV) than for Se(VI). Kinetics data follow the pseudo-second-order model and the obtained parameters k indicate that the rates of adsorption and desorption are higher for Se(VI). Extended X-ray absorption fine structure (EXAFS) and X-ray absorption near edge structure (XANES) analyses reveal that Se is bound at the zeolite surface forming not only the Se-O-Fe but also Se-O-Si bonds. The adsorption mechanism depends of the type of oxo ions. Samples of zeolitic tuff which contain Se were tested as soil supplements for the cultivation of Pleurotus ostreatus mushrooms. The fungus adsorbed the inorganic Se from zeolitic tuff transforming it to a more valuable organically bound form.",
publisher = "Elsevier, Amsterdam",
journal = "Microporous and Mesoporous Materials",
title = "The iron(III)-modified natural zeolitic tuff as an adsorbent and carrier for selenium oxyanions",
pages = "100-92",
volume = "197",
doi = "10.1016/j.micromeso.2014.06.008"
}

Jevtić, S., Arcon, I., Recnik, A., Babić, B. M., Mazaj, M., Pavlović, J., Matijasević, D., Nikšić, M.,& Rajić, N.. (2014). The iron(III)-modified natural zeolitic tuff as an adsorbent and carrier for selenium oxyanions. in Microporous and Mesoporous Materials
Elsevier, Amsterdam., 197, 92-100.
https://doi.org/10.1016/j.micromeso.2014.06.008

Jevtić S, Arcon I, Recnik A, Babić BM, Mazaj M, Pavlović J, Matijasević D, Nikšić M, Rajić N. The iron(III)-modified natural zeolitic tuff as an adsorbent and carrier for selenium oxyanions. in Microporous and Mesoporous Materials. 2014;197:92-100.
doi:10.1016/j.micromeso.2014.06.008 .

Jevtić, Sanja, Arcon, Iztok, Recnik, Aleksander, Babić, Biljana M., Mazaj, Matjai, Pavlović, Jelena, Matijasević, Danka, Nikšić, Miomir, Rajić, Nevenka, "The iron(III)-modified natural zeolitic tuff as an adsorbent and carrier for selenium oxyanions" in Microporous and Mesoporous Materials, 197 (2014):92-100,
https://doi.org/10.1016/j.micromeso.2014.06.008 . .

TY  - JOUR
AU  - Savić, Milena
AU  - Andjelković, I.
AU  - Duvnjak, Dunja
AU  - Matijasević, Danka
AU  - Avramović, Aleksandra
AU  - Nikšić, Miomir
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3061
AB  - The activity of organic selenium against pathogenic molds and its use as a potential selenium source in the production of enriched mushrooms were examined. The effect of commercial selenized yeast on mycelia growth was examined using a method with mycelia disks and a well diffusion method. For mushroom enrichment, different concentrations of selenium were added to a growth substrate. The results presented in this paper suggest that the most suitable concentration of selenized yeast that inhibits the growth of the mycopathogenic molds is 70-100 mg/kg of selenium. With the addition of this concentration to the substrate, mushroom fruit bodies will uptake a high level of selenium, about 100 μg/g for Pleurotus spp., and 200 μg/g for Agaricus bisporus in dry weight of the mushroom. Thereby a double effect in the cultivation of mushrooms is achieved.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.
EP  - 1463
IS  - 4
SP  - 1455
VL  - 64
DO  - 10.2298/ABS1204455S
ER  - 

@article{
author = "Savić, Milena and Andjelković, I. and Duvnjak, Dunja and Matijasević, Danka and Avramović, Aleksandra and Nikšić, Miomir",
year = "2012",
abstract = "The activity of organic selenium against pathogenic molds and its use as a potential selenium source in the production of enriched mushrooms were examined. The effect of commercial selenized yeast on mycelia growth was examined using a method with mycelia disks and a well diffusion method. For mushroom enrichment, different concentrations of selenium were added to a growth substrate. The results presented in this paper suggest that the most suitable concentration of selenized yeast that inhibits the growth of the mycopathogenic molds is 70-100 mg/kg of selenium. With the addition of this concentration to the substrate, mushroom fruit bodies will uptake a high level of selenium, about 100 μg/g for Pleurotus spp., and 200 μg/g for Agaricus bisporus in dry weight of the mushroom. Thereby a double effect in the cultivation of mushrooms is achieved.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.",
pages = "1463-1455",
number = "4",
volume = "64",
doi = "10.2298/ABS1204455S"
}

Savić, M., Andjelković, I., Duvnjak, D., Matijasević, D., Avramović, A.,& Nikšić, M.. (2012). The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1455-1463.
https://doi.org/10.2298/ABS1204455S

Savić M, Andjelković I, Duvnjak D, Matijasević D, Avramović A, Nikšić M. The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.. in Archives of Biological Sciences. 2012;64(4):1455-1463.
doi:10.2298/ABS1204455S .

Savić, Milena, Andjelković, I., Duvnjak, Dunja, Matijasević, Danka, Avramović, Aleksandra, Nikšić, Miomir, "The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp." in Archives of Biological Sciences, 64, no. 4 (2012):1455-1463,
https://doi.org/10.2298/ABS1204455S . .