TY  - CONF
AU  - Stojićević, Aleksandra
AU  - Alimpić, Ana
AU  - Pantić, Milena
AU  - Antić, Mališa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6628
AB  - Basil (Ocimum basilicum L.) has a long traditional use as spice in both folk and conventional medicine. Basil extracts (BEs) as well as essential oil (EO) represents a rich source of phytochemicals, many of which possess diverse biological activities. The objectives of this study were to assess antioxidant and antibacterial activities of BEs and EO, obtained from plants cultivated in Republic of Serbia. EO was isolated by hydrodistillation, while BEs were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. In prepared BEs total phenolic (TPC) and total flavonoid content (TFC) were determined spectrophotometrically. Antioxidant activity was evaluated by DPPH and FRAP assays. A higher TPC was obtained using 70% ethanol, while 96% ethanol proved to be more effective for the extraction of flavonoids. In both antioxidant assays, BEs showed remarkable antioxidant properties compared to EO. Also, regardless the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. Both antioxidant assays strongly correlated with TPC than with TFC. For antibacterial activity assay, minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. Typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes). Opposite to results of antioxidant activity, EO expressed superior antibacterial activity. Based on the obtained results, it can be concluded that basil represents powerful source of biologically active components which can express strong antioxidant and antibacterial activity.
C3  - 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland
T1  - Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts
DO  - 10.3390/ECMC2022-13482
ER  - 

@conference{
author = "Stojićević, Aleksandra and Alimpić, Ana and Pantić, Milena and Antić, Mališa",
year = "2022",
abstract = "Basil (Ocimum basilicum L.) has a long traditional use as spice in both folk and conventional medicine. Basil extracts (BEs) as well as essential oil (EO) represents a rich source of phytochemicals, many of which possess diverse biological activities. The objectives of this study were to assess antioxidant and antibacterial activities of BEs and EO, obtained from plants cultivated in Republic of Serbia. EO was isolated by hydrodistillation, while BEs were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. In prepared BEs total phenolic (TPC) and total flavonoid content (TFC) were determined spectrophotometrically. Antioxidant activity was evaluated by DPPH and FRAP assays. A higher TPC was obtained using 70% ethanol, while 96% ethanol proved to be more effective for the extraction of flavonoids. In both antioxidant assays, BEs showed remarkable antioxidant properties compared to EO. Also, regardless the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. Both antioxidant assays strongly correlated with TPC than with TFC. For antibacterial activity assay, minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. Typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes). Opposite to results of antioxidant activity, EO expressed superior antibacterial activity. Based on the obtained results, it can be concluded that basil represents powerful source of biologically active components which can express strong antioxidant and antibacterial activity.",
journal = "8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland",
title = "Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts",
doi = "10.3390/ECMC2022-13482"
}

Stojićević, A., Alimpić, A., Pantić, M.,& Antić, M.. (2022). Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts. in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland.
https://doi.org/10.3390/ECMC2022-13482

Stojićević A, Alimpić A, Pantić M, Antić M. Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts. in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland. 2022;.
doi:10.3390/ECMC2022-13482 .

Stojićević, Aleksandra, Alimpić, Ana, Pantić, Milena, Antić, Mališa, "Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts" in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland (2022),
https://doi.org/10.3390/ECMC2022-13482 . .

TY  - CONF
AU  - Stojićević, Aleksandra
AU  - Alimpić Aradski, Ana
AU  - Pantić, Milena
AU  - Pantelić, Nebojša
AU  - Rabrenović, Biljana
AU  - Duletić-Laušević, Sonja
AU  - Antić, Mališa
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6630
AB  - Medicinal plants represent a rich source of phytochemicals, many of which possess diverse biological activities, including antioxidants. The objectives of this study were to assess antioxidant, antibacterial and anticancer activities of savory (Satureja montana L.) ethanolic extracts and to monitor the changes of oxidative status during long-term storage conditions of the cold pressed sunflower oil (CPSU) with the addition of extracts at various concentrations.  Extracts were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. Antioxidant activity was evaluated by DPPH and ꞵ-carotene bleaching assays. In both assays, extracts showed remarkable antioxidative properties which were equal or stronger than those of BHT and BHA. Also, regardless of the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. For antibacterial activity assay, minimal inhibitory concentrations (MIC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes) with MIC=1,25–10 μg/mL and 0,0781–0,3125 μg/mL, respectively. As results obtained by MTT test, the extracts didn’t show anticancer activity (IC50>100 μg/L) against tested tumor cell lines: PC-3 (prostate), HT-29 (colon) and HeLa (cervix) in the applied concentration range. For investigation of oxidative stability of CPSU, only 70% ethanol extracts were used. Samples of CPSU (with addition of 250, 500 and 1000 ppm of extracts), as well as control samples (with addition of 200 ppm of BHT and CPSU without any additives), were stored at room temperature for six months. In order to determine the level of oxidative changes, samples were analyzed for peroxide, anisidine value and conjugated dienes and trienes. Based on obtained results, it can be concluded that savory extracts are powerful natural antioxidants that can delay the oxidative deterioration of CPSU and whose effectiveness is comparable to the synthetic antioxidant BHT.
PB  - University of Belgrade
C3  - UniFood Conference
T1  - Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6630
ER  - 

@conference{
author = "Stojićević, Aleksandra and Alimpić Aradski, Ana and Pantić, Milena and Pantelić, Nebojša and Rabrenović, Biljana and Duletić-Laušević, Sonja and Antić, Mališa and Nikšić, Miomir",
year = "2021",
abstract = "Medicinal plants represent a rich source of phytochemicals, many of which possess diverse biological activities, including antioxidants. The objectives of this study were to assess antioxidant, antibacterial and anticancer activities of savory (Satureja montana L.) ethanolic extracts and to monitor the changes of oxidative status during long-term storage conditions of the cold pressed sunflower oil (CPSU) with the addition of extracts at various concentrations.  Extracts were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. Antioxidant activity was evaluated by DPPH and ꞵ-carotene bleaching assays. In both assays, extracts showed remarkable antioxidative properties which were equal or stronger than those of BHT and BHA. Also, regardless of the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. For antibacterial activity assay, minimal inhibitory concentrations (MIC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes) with MIC=1,25–10 μg/mL and 0,0781–0,3125 μg/mL, respectively. As results obtained by MTT test, the extracts didn’t show anticancer activity (IC50>100 μg/L) against tested tumor cell lines: PC-3 (prostate), HT-29 (colon) and HeLa (cervix) in the applied concentration range. For investigation of oxidative stability of CPSU, only 70% ethanol extracts were used. Samples of CPSU (with addition of 250, 500 and 1000 ppm of extracts), as well as control samples (with addition of 200 ppm of BHT and CPSU without any additives), were stored at room temperature for six months. In order to determine the level of oxidative changes, samples were analyzed for peroxide, anisidine value and conjugated dienes and trienes. Based on obtained results, it can be concluded that savory extracts are powerful natural antioxidants that can delay the oxidative deterioration of CPSU and whose effectiveness is comparable to the synthetic antioxidant BHT.",
publisher = "University of Belgrade",
journal = "UniFood Conference",
title = "Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6630"
}

Stojićević, A., Alimpić Aradski, A., Pantić, M., Pantelić, N., Rabrenović, B., Duletić-Laušević, S., Antić, M.,& Nikšić, M.. (2021). Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions. in UniFood Conference
University of Belgrade., 101.
https://hdl.handle.net/21.15107/rcub_agrospace_6630

Stojićević A, Alimpić Aradski A, Pantić M, Pantelić N, Rabrenović B, Duletić-Laušević S, Antić M, Nikšić M. Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions. in UniFood Conference. 2021;:101.
https://hdl.handle.net/21.15107/rcub_agrospace_6630 .

Stojićević, Aleksandra, Alimpić Aradski, Ana, Pantić, Milena, Pantelić, Nebojša, Rabrenović, Biljana, Duletić-Laušević, Sonja, Antić, Mališa, Nikšić, Miomir, "Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions" in UniFood Conference (2021):101,
https://hdl.handle.net/21.15107/rcub_agrospace_6630 .