Characterisation of polypeptide composition of various soya bean genotypes
Karakterizacija polipeptidnog sastava različitih genotipova soje
Апстракт
The polypeptide composition of soluble proteins of three soya bean genotypes - Nena, ZPS-015 and L91-4042, the latter without Kunitz trypsin inhibitor were studied. SDS-polyacrilamid gel electrophoreses and scanning densitometry of the obtained gels were used to estimate the polypeptide composition. Polypeptide analyses indicated that soybean genotypes express significant differences in both, the protein structure and the protein complex. The lowest content of two major storage proteins, β-conglycinin (7S) and glycinin (11S) was detected in the line without Kunitz trypsin inhibitor (19.49% i 42.13% respectively), the highest β-conglycinin content was in the line ZPS-015 (24.60%) and the glycinine content in the line Nena (45.06%). One polypeptide fraction with a molecular weight close to the molecular weight of γ-conglycinin was noticed only in the soybean genotype L91-4042. The various magnitude in the protein complex was observed in the γ-conglycinin content. The differences in the p...rotein content and the protein structure was also observed in minor protein fractions. The difference in the polypeptide composition of the investigated genotypes was related to previous investigations of soya bean cheese production. The solublity of the soybean protein and the 11S:7S ration has strong influence to the mass and the quality of tofu. Very strong negative correlation exists between the 11S:7S ration and the mass of tofu. Understanding the soya bean genotype protein structure could contribute to characterisation of the existing soya bean lines and to selection of the new soya bean lines with specific functional properties. It is very important for soya bean processing and utilisation.
U radu je proučavan polipeptidni sastav rastvorljivih proteina tri genotipa soje - Nena, ZPS-015 i L91-4042. Utvrđivanje polipeptidnog sastava izvršeno je SDS-poliakrilamidnomnom gel elektroforezom i HPLC tehnikom. Polipeptidna analiza je pokazala da sorte soje ispoljavaju značajne genotipske razlike u strukturi proteina i proteinskog kompleksa. Najniži sadržaj dva glavna rezervna proteina soje, β-konglicinina i glicinina detektovan je kod sorte bez Kunitz-ovog tripsin inhibitora (19,49%, odnosno 42,13%) dok je kod sorte ZPS-015 uočen najviši sadržaj β-konglicinin (24,60%), a glicinina je bilo najviše kod genotipa Nena (45.06%). Kod sorte L91-4042 uočena je jedna polipeptidna frakcija čija je molekulska masa bliska γ-konglicininu, a nije prisutna kod ostala dva genotipa. Heterogenost sastava i sadržaja proteinskog kompleksa ispoljen je i kod manje zastupljenih proteinskih frakcija. Okarakterisane genotipske razlike u strukturi proteinskog kompleksa dovedene su u vezu sa ranijim istraži...vanjima veznim za proizvodnju sojinog sira. Utvrđeno je da na prinos i kvalitet sojinog sira utiče rastvorljivost proteina i odnos glicinin: β konglicinin.
Кључне речи:
β-konglicinin / glicinin / polipeptidni sastav / proteini soje / rastvorljivostИзвор:
Journal of Scientific Agricultural Research, 2003, 64, 1-2, 157-165Издавач:
- Savez poljoprivrednih inženjera i tehničara, Beograd
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Pešić, Mirjana AU - Vučelić-Radović, Biljana AU - Barać, Miroljub PY - 2003 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/657 AB - The polypeptide composition of soluble proteins of three soya bean genotypes - Nena, ZPS-015 and L91-4042, the latter without Kunitz trypsin inhibitor were studied. SDS-polyacrilamid gel electrophoreses and scanning densitometry of the obtained gels were used to estimate the polypeptide composition. Polypeptide analyses indicated that soybean genotypes express significant differences in both, the protein structure and the protein complex. The lowest content of two major storage proteins, β-conglycinin (7S) and glycinin (11S) was detected in the line without Kunitz trypsin inhibitor (19.49% i 42.13% respectively), the highest β-conglycinin content was in the line ZPS-015 (24.60%) and the glycinine content in the line Nena (45.06%). One polypeptide fraction with a molecular weight close to the molecular weight of γ-conglycinin was noticed only in the soybean genotype L91-4042. The various magnitude in the protein complex was observed in the γ-conglycinin content. The differences in the protein content and the protein structure was also observed in minor protein fractions. The difference in the polypeptide composition of the investigated genotypes was related to previous investigations of soya bean cheese production. The solublity of the soybean protein and the 11S:7S ration has strong influence to the mass and the quality of tofu. Very strong negative correlation exists between the 11S:7S ration and the mass of tofu. Understanding the soya bean genotype protein structure could contribute to characterisation of the existing soya bean lines and to selection of the new soya bean lines with specific functional properties. It is very important for soya bean processing and utilisation. AB - U radu je proučavan polipeptidni sastav rastvorljivih proteina tri genotipa soje - Nena, ZPS-015 i L91-4042. Utvrđivanje polipeptidnog sastava izvršeno je SDS-poliakrilamidnomnom gel elektroforezom i HPLC tehnikom. Polipeptidna analiza je pokazala da sorte soje ispoljavaju značajne genotipske razlike u strukturi proteina i proteinskog kompleksa. Najniži sadržaj dva glavna rezervna proteina soje, β-konglicinina i glicinina detektovan je kod sorte bez Kunitz-ovog tripsin inhibitora (19,49%, odnosno 42,13%) dok je kod sorte ZPS-015 uočen najviši sadržaj β-konglicinin (24,60%), a glicinina je bilo najviše kod genotipa Nena (45.06%). Kod sorte L91-4042 uočena je jedna polipeptidna frakcija čija je molekulska masa bliska γ-konglicininu, a nije prisutna kod ostala dva genotipa. Heterogenost sastava i sadržaja proteinskog kompleksa ispoljen je i kod manje zastupljenih proteinskih frakcija. Okarakterisane genotipske razlike u strukturi proteinskog kompleksa dovedene su u vezu sa ranijim istraživanjima veznim za proizvodnju sojinog sira. Utvrđeno je da na prinos i kvalitet sojinog sira utiče rastvorljivost proteina i odnos glicinin: β konglicinin. PB - Savez poljoprivrednih inženjera i tehničara, Beograd T2 - Journal of Scientific Agricultural Research T1 - Characterisation of polypeptide composition of various soya bean genotypes T1 - Karakterizacija polipeptidnog sastava različitih genotipova soje EP - 165 IS - 1-2 SP - 157 VL - 64 UR - https://hdl.handle.net/21.15107/rcub_agrospace_657 ER -
@article{ author = "Pešić, Mirjana and Vučelić-Radović, Biljana and Barać, Miroljub", year = "2003", abstract = "The polypeptide composition of soluble proteins of three soya bean genotypes - Nena, ZPS-015 and L91-4042, the latter without Kunitz trypsin inhibitor were studied. SDS-polyacrilamid gel electrophoreses and scanning densitometry of the obtained gels were used to estimate the polypeptide composition. Polypeptide analyses indicated that soybean genotypes express significant differences in both, the protein structure and the protein complex. The lowest content of two major storage proteins, β-conglycinin (7S) and glycinin (11S) was detected in the line without Kunitz trypsin inhibitor (19.49% i 42.13% respectively), the highest β-conglycinin content was in the line ZPS-015 (24.60%) and the glycinine content in the line Nena (45.06%). One polypeptide fraction with a molecular weight close to the molecular weight of γ-conglycinin was noticed only in the soybean genotype L91-4042. The various magnitude in the protein complex was observed in the γ-conglycinin content. The differences in the protein content and the protein structure was also observed in minor protein fractions. The difference in the polypeptide composition of the investigated genotypes was related to previous investigations of soya bean cheese production. The solublity of the soybean protein and the 11S:7S ration has strong influence to the mass and the quality of tofu. Very strong negative correlation exists between the 11S:7S ration and the mass of tofu. Understanding the soya bean genotype protein structure could contribute to characterisation of the existing soya bean lines and to selection of the new soya bean lines with specific functional properties. It is very important for soya bean processing and utilisation., U radu je proučavan polipeptidni sastav rastvorljivih proteina tri genotipa soje - Nena, ZPS-015 i L91-4042. Utvrđivanje polipeptidnog sastava izvršeno je SDS-poliakrilamidnomnom gel elektroforezom i HPLC tehnikom. Polipeptidna analiza je pokazala da sorte soje ispoljavaju značajne genotipske razlike u strukturi proteina i proteinskog kompleksa. Najniži sadržaj dva glavna rezervna proteina soje, β-konglicinina i glicinina detektovan je kod sorte bez Kunitz-ovog tripsin inhibitora (19,49%, odnosno 42,13%) dok je kod sorte ZPS-015 uočen najviši sadržaj β-konglicinin (24,60%), a glicinina je bilo najviše kod genotipa Nena (45.06%). Kod sorte L91-4042 uočena je jedna polipeptidna frakcija čija je molekulska masa bliska γ-konglicininu, a nije prisutna kod ostala dva genotipa. Heterogenost sastava i sadržaja proteinskog kompleksa ispoljen je i kod manje zastupljenih proteinskih frakcija. Okarakterisane genotipske razlike u strukturi proteinskog kompleksa dovedene su u vezu sa ranijim istraživanjima veznim za proizvodnju sojinog sira. Utvrđeno je da na prinos i kvalitet sojinog sira utiče rastvorljivost proteina i odnos glicinin: β konglicinin.", publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd", journal = "Journal of Scientific Agricultural Research", title = "Characterisation of polypeptide composition of various soya bean genotypes, Karakterizacija polipeptidnog sastava različitih genotipova soje", pages = "165-157", number = "1-2", volume = "64", url = "https://hdl.handle.net/21.15107/rcub_agrospace_657" }
Pešić, M., Vučelić-Radović, B.,& Barać, M.. (2003). Characterisation of polypeptide composition of various soya bean genotypes. in Journal of Scientific Agricultural Research Savez poljoprivrednih inženjera i tehničara, Beograd., 64(1-2), 157-165. https://hdl.handle.net/21.15107/rcub_agrospace_657
Pešić M, Vučelić-Radović B, Barać M. Characterisation of polypeptide composition of various soya bean genotypes. in Journal of Scientific Agricultural Research. 2003;64(1-2):157-165. https://hdl.handle.net/21.15107/rcub_agrospace_657 .
Pešić, Mirjana, Vučelić-Radović, Biljana, Barać, Miroljub, "Characterisation of polypeptide composition of various soya bean genotypes" in Journal of Scientific Agricultural Research, 64, no. 1-2 (2003):157-165, https://hdl.handle.net/21.15107/rcub_agrospace_657 .