Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces
Samo za registrovane korisnike
2023
Autori
Cvetković, StefanaTenji, Dina
Mitić-Ćulafić, Dragana
Vuletić, Stefana
Ganić, Tea
Djekić, Ilija
Nikolić, Biljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against qu...orum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd
Ključne reči:
Ambient temperature / Antibiofilm effect / Food contact surfaces / Foodborne pathogens / Listeria monocytogenes / Yellow gentianIzvor:
Food Bioscience, 2023, 54Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200125 (Univerzitet u Novom Sadu, Prirodno-matematički fakultet) (RS-MESTD-inst-2020-200125)
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Cvetković, Stefana AU - Tenji, Dina AU - Mitić-Ćulafić, Dragana AU - Vuletić, Stefana AU - Ganić, Tea AU - Djekić, Ilija AU - Nikolić, Biljana PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6377 AB - As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against quorum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd T2 - Food Bioscience T2 - Food Bioscience T1 - Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces VL - 54 DO - 10.1016/j.fbio.2023.102857 ER -
@article{ author = "Cvetković, Stefana and Tenji, Dina and Mitić-Ćulafić, Dragana and Vuletić, Stefana and Ganić, Tea and Djekić, Ilija and Nikolić, Biljana", year = "2023", abstract = "As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against quorum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd", journal = "Food Bioscience, Food Bioscience", title = "Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces", volume = "54", doi = "10.1016/j.fbio.2023.102857" }
Cvetković, S., Tenji, D., Mitić-Ćulafić, D., Vuletić, S., Ganić, T., Djekić, I.,& Nikolić, B.. (2023). Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces. in Food Bioscience, 54. https://doi.org/10.1016/j.fbio.2023.102857
Cvetković S, Tenji D, Mitić-Ćulafić D, Vuletić S, Ganić T, Djekić I, Nikolić B. Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces. in Food Bioscience. 2023;54. doi:10.1016/j.fbio.2023.102857 .
Cvetković, Stefana, Tenji, Dina, Mitić-Ćulafić, Dragana, Vuletić, Stefana, Ganić, Tea, Djekić, Ilija, Nikolić, Biljana, "Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces" in Food Bioscience, 54 (2023), https://doi.org/10.1016/j.fbio.2023.102857 . .