Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
Само за регистроване кориснике
2023
Аутори
Kostić-Vuković, JovanaKolarević, Stoimir
Sunjog, Karolina
Subotić, Srđan
Višnjić-Jeftić, Željka
Rašković, Božidar
Poleksić, Vesna
Vuković-Gačić, Branka
Lenhardt, Mirjana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a sign...ificantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Кључне речи:
Comet assay / Erythrocyte morphometry / HepG2 / Histopathology / Metals accumulation / MicronucleusИзвор:
Ecotoxicology, 2023Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Kostić-Vuković, Jovana AU - Kolarević, Stoimir AU - Sunjog, Karolina AU - Subotić, Srđan AU - Višnjić-Jeftić, Željka AU - Rašković, Božidar AU - Poleksić, Vesna AU - Vuković-Gačić, Branka AU - Lenhardt, Mirjana PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6354 AB - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. T2 - Ecotoxicology T2 - Ecotoxicology T1 - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia DO - 10.1007/s10646-023-02663-6 ER -
@article{ author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić-Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana", year = "2023", abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.", journal = "Ecotoxicology, Ecotoxicology", title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia", doi = "10.1007/s10646-023-02663-6" }
Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić-Jeftić, Ž., Rašković, B., Poleksić, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology. https://doi.org/10.1007/s10646-023-02663-6
Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić-Jeftić Ž, Rašković B, Poleksić V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology. 2023;. doi:10.1007/s10646-023-02663-6 .
Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić-Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Ecotoxicology (2023), https://doi.org/10.1007/s10646-023-02663-6 . .