Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout
Аутори
Gruber-Dorninger, ChristianeKillinger, Manuela
Höbartner-Gußl, Andreas
Rosen, Roy
Doupovec, Barbara
Aleschko, Markus
Schwartz-Zimmermann, Heidi
Greitbauer, Oliver
Marković, Zoran
Stanković, Marko
Schöndorfer, Karin
Vukmirovic, Djuro
Wein, Silvia
Schatzmayr, Dian
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The estrogenic mycotoxin zearalenone (ZEN) is a common contaminant of animal feed. Effective strategies for the inactivation of ZEN in feed are required. The ZEN-degrading enzyme zearalenone hydrolase ZenA (EC 3.1.1.-, commercial name ZENzyme®, BIOMIN Holding GmbH, Getzersdorf, Austria) converts ZEN to hydrolyzed ZEN (HZEN), thereby enabling a strong reduction in estrogenicity. In this study, we investigated the efficacy of ZenA added to feed to degrade ZEN in the gastrointestinal tract of three monogastric animal species, i.e., pigs, chickens, and rainbow trout. For each species, groups of animals received (i) feed contaminated with ZEN (chickens: 400 µg/kg, pigs: 200 µg/kg, rainbow trout: 2000 µg/kg), (ii) feed contaminated with ZEN and supplemented with ZenA, or (iii) uncontaminated feed. To investigate the fate of dietary ZEN in the gastrointestinal tract in the presence and absence of ZenA, concentrations of ZEN and ZEN metabolites were analyzed in digesta of chickens and rainbow ...trout and in feces of pigs. Upon ZenA administration, concentrations of ZEN were significantly decreased and concentrations of the degradation product HZEN were significantly increased in digesta/feces of each investigated animal species, indicating degradation of ZEN by ZenA in the gastrointestinal tract. Moreover, upon addition of ZenA to the diet, the concentration of the highly estrogenic ZEN metabolite α-ZEL was significantly reduced in feces of pigs. In conclusion, ZenA was effective in degrading ZEN to HZEN in the gastrointestinal tract of chickens, pigs, and rainbow trout, and counteracted formation of α-ZEL in pigs. Therefore, ZenA could find application as a ZEN-degrading feed additive for these animal species. © 2023 by the authors.
Кључне речи:
chicken / degradation / enzyme / feed additive / fish / gastrointestinal / hydrolase / rainbow trout / swine / zearalenoneИзвор:
Toxins, 2023, 15, 1Институција/група
Poljoprivredni fakultetTY - JOUR AU - Gruber-Dorninger, Christiane AU - Killinger, Manuela AU - Höbartner-Gußl, Andreas AU - Rosen, Roy AU - Doupovec, Barbara AU - Aleschko, Markus AU - Schwartz-Zimmermann, Heidi AU - Greitbauer, Oliver AU - Marković, Zoran AU - Stanković, Marko AU - Schöndorfer, Karin AU - Vukmirovic, Djuro AU - Wein, Silvia AU - Schatzmayr, Dian PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6272 AB - The estrogenic mycotoxin zearalenone (ZEN) is a common contaminant of animal feed. Effective strategies for the inactivation of ZEN in feed are required. The ZEN-degrading enzyme zearalenone hydrolase ZenA (EC 3.1.1.-, commercial name ZENzyme®, BIOMIN Holding GmbH, Getzersdorf, Austria) converts ZEN to hydrolyzed ZEN (HZEN), thereby enabling a strong reduction in estrogenicity. In this study, we investigated the efficacy of ZenA added to feed to degrade ZEN in the gastrointestinal tract of three monogastric animal species, i.e., pigs, chickens, and rainbow trout. For each species, groups of animals received (i) feed contaminated with ZEN (chickens: 400 µg/kg, pigs: 200 µg/kg, rainbow trout: 2000 µg/kg), (ii) feed contaminated with ZEN and supplemented with ZenA, or (iii) uncontaminated feed. To investigate the fate of dietary ZEN in the gastrointestinal tract in the presence and absence of ZenA, concentrations of ZEN and ZEN metabolites were analyzed in digesta of chickens and rainbow trout and in feces of pigs. Upon ZenA administration, concentrations of ZEN were significantly decreased and concentrations of the degradation product HZEN were significantly increased in digesta/feces of each investigated animal species, indicating degradation of ZEN by ZenA in the gastrointestinal tract. Moreover, upon addition of ZenA to the diet, the concentration of the highly estrogenic ZEN metabolite α-ZEL was significantly reduced in feces of pigs. In conclusion, ZenA was effective in degrading ZEN to HZEN in the gastrointestinal tract of chickens, pigs, and rainbow trout, and counteracted formation of α-ZEL in pigs. Therefore, ZenA could find application as a ZEN-degrading feed additive for these animal species. © 2023 by the authors. T2 - Toxins T2 - Toxins T1 - Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout IS - 1 VL - 15 DO - 10.3390/toxins15010048 ER -
@article{ author = "Gruber-Dorninger, Christiane and Killinger, Manuela and Höbartner-Gußl, Andreas and Rosen, Roy and Doupovec, Barbara and Aleschko, Markus and Schwartz-Zimmermann, Heidi and Greitbauer, Oliver and Marković, Zoran and Stanković, Marko and Schöndorfer, Karin and Vukmirovic, Djuro and Wein, Silvia and Schatzmayr, Dian", year = "2023", abstract = "The estrogenic mycotoxin zearalenone (ZEN) is a common contaminant of animal feed. Effective strategies for the inactivation of ZEN in feed are required. The ZEN-degrading enzyme zearalenone hydrolase ZenA (EC 3.1.1.-, commercial name ZENzyme®, BIOMIN Holding GmbH, Getzersdorf, Austria) converts ZEN to hydrolyzed ZEN (HZEN), thereby enabling a strong reduction in estrogenicity. In this study, we investigated the efficacy of ZenA added to feed to degrade ZEN in the gastrointestinal tract of three monogastric animal species, i.e., pigs, chickens, and rainbow trout. For each species, groups of animals received (i) feed contaminated with ZEN (chickens: 400 µg/kg, pigs: 200 µg/kg, rainbow trout: 2000 µg/kg), (ii) feed contaminated with ZEN and supplemented with ZenA, or (iii) uncontaminated feed. To investigate the fate of dietary ZEN in the gastrointestinal tract in the presence and absence of ZenA, concentrations of ZEN and ZEN metabolites were analyzed in digesta of chickens and rainbow trout and in feces of pigs. Upon ZenA administration, concentrations of ZEN were significantly decreased and concentrations of the degradation product HZEN were significantly increased in digesta/feces of each investigated animal species, indicating degradation of ZEN by ZenA in the gastrointestinal tract. Moreover, upon addition of ZenA to the diet, the concentration of the highly estrogenic ZEN metabolite α-ZEL was significantly reduced in feces of pigs. In conclusion, ZenA was effective in degrading ZEN to HZEN in the gastrointestinal tract of chickens, pigs, and rainbow trout, and counteracted formation of α-ZEL in pigs. Therefore, ZenA could find application as a ZEN-degrading feed additive for these animal species. © 2023 by the authors.", journal = "Toxins, Toxins", title = "Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout", number = "1", volume = "15", doi = "10.3390/toxins15010048" }
Gruber-Dorninger, C., Killinger, M., Höbartner-Gußl, A., Rosen, R., Doupovec, B., Aleschko, M., Schwartz-Zimmermann, H., Greitbauer, O., Marković, Z., Stanković, M., Schöndorfer, K., Vukmirovic, D., Wein, S.,& Schatzmayr, D.. (2023). Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout. in Toxins, 15(1). https://doi.org/10.3390/toxins15010048
Gruber-Dorninger C, Killinger M, Höbartner-Gußl A, Rosen R, Doupovec B, Aleschko M, Schwartz-Zimmermann H, Greitbauer O, Marković Z, Stanković M, Schöndorfer K, Vukmirovic D, Wein S, Schatzmayr D. Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout. in Toxins. 2023;15(1). doi:10.3390/toxins15010048 .
Gruber-Dorninger, Christiane, Killinger, Manuela, Höbartner-Gußl, Andreas, Rosen, Roy, Doupovec, Barbara, Aleschko, Markus, Schwartz-Zimmermann, Heidi, Greitbauer, Oliver, Marković, Zoran, Stanković, Marko, Schöndorfer, Karin, Vukmirovic, Djuro, Wein, Silvia, Schatzmayr, Dian, "Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout" in Toxins, 15, no. 1 (2023), https://doi.org/10.3390/toxins15010048 . .