Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia
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2022
Authors
Stanković, IvanaZečević, Katarina
Delibašić, Goran
Jović, Jelena
Toševski, Ivo
Krstić, Branka
Article (Published version)
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Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected
grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in
Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing
reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed
that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp
that confrmed the presence of GRSPaV. No amplifcation products were recorded in samples prepared from basal leaves
or petioles. A serological assay confrmed the presence of GRSPaV in all tested mature cane samples. For further analyses,
the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially
sequenced and six sequence varia...nts were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage.
This is the frst report of GRSPaV present in Serbia. Further monitoring and in
Keywords:
Grapevine / Grapevine rupestris stem pitting-associated virus / Multiplex RT-PCR / CP gene / Phylogenetic analysisSource:
Journal of Plant Diseases and Protection, 2022, 1, 1Publisher:
- Springer
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-MESTD-inst-2020-200010)
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Poljoprivredni fakultetTY - JOUR AU - Stanković, Ivana AU - Zečević, Katarina AU - Delibašić, Goran AU - Jović, Jelena AU - Toševski, Ivo AU - Krstić, Branka PY - 2022 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6155 AB - Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confrmed the presence of GRSPaV. No amplifcation products were recorded in samples prepared from basal leaves or petioles. A serological assay confrmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the frst report of GRSPaV present in Serbia. Further monitoring and in PB - Springer T2 - Journal of Plant Diseases and Protection T1 - Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia IS - 1 VL - 1 DO - 10.1007/s41348-022-00656-x ER -
@article{ author = "Stanković, Ivana and Zečević, Katarina and Delibašić, Goran and Jović, Jelena and Toševski, Ivo and Krstić, Branka", year = "2022", abstract = "Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confrmed the presence of GRSPaV. No amplifcation products were recorded in samples prepared from basal leaves or petioles. A serological assay confrmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the frst report of GRSPaV present in Serbia. Further monitoring and in", publisher = "Springer", journal = "Journal of Plant Diseases and Protection", title = "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia", number = "1", volume = "1", doi = "10.1007/s41348-022-00656-x" }
Stanković, I., Zečević, K., Delibašić, G., Jović, J., Toševski, I.,& Krstić, B.. (2022). Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in Journal of Plant Diseases and Protection Springer., 1(1). https://doi.org/10.1007/s41348-022-00656-x
Stanković I, Zečević K, Delibašić G, Jović J, Toševski I, Krstić B. Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in Journal of Plant Diseases and Protection. 2022;1(1). doi:10.1007/s41348-022-00656-x .
Stanković, Ivana, Zečević, Katarina, Delibašić, Goran, Jović, Jelena, Toševski, Ivo, Krstić, Branka, "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia" in Journal of Plant Diseases and Protection, 1, no. 1 (2022), https://doi.org/10.1007/s41348-022-00656-x . .