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Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor
dc.creator | Miletić, Dunja | |
dc.creator | Turło, Jadwiga | |
dc.creator | Podsadni, Piotr | |
dc.creator | Sknepnek, Aleksandra | |
dc.creator | Szczepanska, Agnieszka | |
dc.creator | Klimaszewska, Marzenna | |
dc.creator | Malinowska, Eliza | |
dc.creator | Lević, Steva | |
dc.creator | Nedović, Viktor | |
dc.creator | Nikšić, Miomir | |
dc.date.accessioned | 2021-04-26T08:37:41Z | |
dc.date.available | 2021-04-26T08:37:41Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2212-4292 | |
dc.identifier.uri | http://aspace.agrif.bg.ac.rs/handle/123456789/5836 | |
dc.description.abstract | During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier Ltd | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200116/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Food Bioscience | sr |
dc.subject | Antibacterial activity | sr |
dc.subject | Antioxidative activity | sr |
dc.subject | Exopolysaccharides | sr |
dc.subject | Mushrooms | sr |
dc.subject | Selenium | sr |
dc.subject | Submerged cultivation | sr |
dc.title | Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.citation.rank | M21 | |
dc.citation.spage | 101046 | |
dc.citation.volume | 42 | |
dc.identifier.doi | 10.1016/j.fbio.2021.101046 | |
dc.identifier.scopus | 2-s2.0-85104050605 | |
dc.identifier.wos | 000687781400010 | |
dc.type.version | publishedVersion | sr |