Insecticidal Activity of Sage (Salvia Officinalis) Essential Oil to Varroa Destructor (Acari: Varroidae) and Apis Mellifera (Hymenoptera: Apidae)
Апстракт
The need to find alternative systems of the fight against Varroa mite without application of chemicals and provide healthy bee products resulted in investigation of application of different plant essences to arthropod control. In order to perceive the sage essential oil (Salvia officinalis) bioactivity, contact residual toxicity of mites and bees was examined in the laboratory conditions. The chemical composition of essential oil was determined by standard GC and GC/MS methods. Different doses of the sage essential oil dissolved in acetone (0.1–10 μl/Petri dish) were applied in Petri dishes and left to dry for 20 minute at a room temperature. Following this period of time, ten honey bees and five adult female mites were added in each Petri dish and they were all maintained in controlled conditions (T = 30°C, Relative humidity = 60%). Survival of examined honey bees and Varroa mites was recorded two times, after 24 h and 48 h. The most prominent toxic effect on the examined Varroa mites... was observed after 24 h and 48 h, with application of 10 μl of sage oil (the average values for dead mite individuals were 3.25 and 3.50, respectively). Recorded biological activities of the oil tested in different doses on both honey bee and Varroa mite revealed opportunity to proceed with further investigation by selecting the most appropriate variants and combinations of the most prominent individual components of the examined sage oil.
Кључне речи:
essential oil / Apis mellifera / Varroa miteИзвор:
International Symposium on Animal Science 2014, 23-25th September 2014, Belgrade, Serbia, 2014Финансирање / пројекти:
- Развој интегрисаних система управљања штетним организмима у биљној производњи са циљем превазилажења резистентности и унапређења квалитета и безбедности хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
- Унапређење и развој хигијенских и технолошких поступака у производњи намирница животињског порекла у циљу добијања квалитетних и безбедних производа конкурентних на светском тржишту (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
Институција/група
Poljoprivredni fakultetTY - CONF AU - Nedić, Nebojša AU - Kostić, M AU - Marković, T AU - Marković, M AU - Jevtić, G AU - Andjelković, B PY - 2014 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5538 AB - The need to find alternative systems of the fight against Varroa mite without application of chemicals and provide healthy bee products resulted in investigation of application of different plant essences to arthropod control. In order to perceive the sage essential oil (Salvia officinalis) bioactivity, contact residual toxicity of mites and bees was examined in the laboratory conditions. The chemical composition of essential oil was determined by standard GC and GC/MS methods. Different doses of the sage essential oil dissolved in acetone (0.1–10 μl/Petri dish) were applied in Petri dishes and left to dry for 20 minute at a room temperature. Following this period of time, ten honey bees and five adult female mites were added in each Petri dish and they were all maintained in controlled conditions (T = 30°C, Relative humidity = 60%). Survival of examined honey bees and Varroa mites was recorded two times, after 24 h and 48 h. The most prominent toxic effect on the examined Varroa mites was observed after 24 h and 48 h, with application of 10 μl of sage oil (the average values for dead mite individuals were 3.25 and 3.50, respectively). Recorded biological activities of the oil tested in different doses on both honey bee and Varroa mite revealed opportunity to proceed with further investigation by selecting the most appropriate variants and combinations of the most prominent individual components of the examined sage oil. C3 - International Symposium on Animal Science 2014, 23-25th September 2014, Belgrade, Serbia T1 - Insecticidal Activity of Sage (Salvia Officinalis) Essential Oil to Varroa Destructor (Acari: Varroidae) and Apis Mellifera (Hymenoptera: Apidae) UR - https://hdl.handle.net/21.15107/rcub_agrospace_5538 ER -
@conference{ author = "Nedić, Nebojša and Kostić, M and Marković, T and Marković, M and Jevtić, G and Andjelković, B", year = "2014", abstract = "The need to find alternative systems of the fight against Varroa mite without application of chemicals and provide healthy bee products resulted in investigation of application of different plant essences to arthropod control. In order to perceive the sage essential oil (Salvia officinalis) bioactivity, contact residual toxicity of mites and bees was examined in the laboratory conditions. The chemical composition of essential oil was determined by standard GC and GC/MS methods. Different doses of the sage essential oil dissolved in acetone (0.1–10 μl/Petri dish) were applied in Petri dishes and left to dry for 20 minute at a room temperature. Following this period of time, ten honey bees and five adult female mites were added in each Petri dish and they were all maintained in controlled conditions (T = 30°C, Relative humidity = 60%). Survival of examined honey bees and Varroa mites was recorded two times, after 24 h and 48 h. The most prominent toxic effect on the examined Varroa mites was observed after 24 h and 48 h, with application of 10 μl of sage oil (the average values for dead mite individuals were 3.25 and 3.50, respectively). Recorded biological activities of the oil tested in different doses on both honey bee and Varroa mite revealed opportunity to proceed with further investigation by selecting the most appropriate variants and combinations of the most prominent individual components of the examined sage oil.", journal = "International Symposium on Animal Science 2014, 23-25th September 2014, Belgrade, Serbia", title = "Insecticidal Activity of Sage (Salvia Officinalis) Essential Oil to Varroa Destructor (Acari: Varroidae) and Apis Mellifera (Hymenoptera: Apidae)", url = "https://hdl.handle.net/21.15107/rcub_agrospace_5538" }
Nedić, N., Kostić, M., Marković, T., Marković, M., Jevtić, G.,& Andjelković, B.. (2014). Insecticidal Activity of Sage (Salvia Officinalis) Essential Oil to Varroa Destructor (Acari: Varroidae) and Apis Mellifera (Hymenoptera: Apidae). in International Symposium on Animal Science 2014, 23-25th September 2014, Belgrade, Serbia. https://hdl.handle.net/21.15107/rcub_agrospace_5538
Nedić N, Kostić M, Marković T, Marković M, Jevtić G, Andjelković B. Insecticidal Activity of Sage (Salvia Officinalis) Essential Oil to Varroa Destructor (Acari: Varroidae) and Apis Mellifera (Hymenoptera: Apidae). in International Symposium on Animal Science 2014, 23-25th September 2014, Belgrade, Serbia. 2014;. https://hdl.handle.net/21.15107/rcub_agrospace_5538 .
Nedić, Nebojša, Kostić, M, Marković, T, Marković, M, Jevtić, G, Andjelković, B, "Insecticidal Activity of Sage (Salvia Officinalis) Essential Oil to Varroa Destructor (Acari: Varroidae) and Apis Mellifera (Hymenoptera: Apidae)" in International Symposium on Animal Science 2014, 23-25th September 2014, Belgrade, Serbia (2014), https://hdl.handle.net/21.15107/rcub_agrospace_5538 .