Приказ основних података о документу
Fluorescence analysis of liposomal membranes permeability
Fluorimetrijska analiza permeabilnosti lipozomalnih membrana
dc.creator | Jovanović, Aleksandra A. | |
dc.creator | Balanc, Bojana | |
dc.creator | Djordjević, Verica B. | |
dc.creator | Savikin, Katarina | |
dc.creator | Nedović, Viktor | |
dc.creator | Bugarski, Branko | |
dc.creator | Poklar-Ulrih, Nataša | |
dc.date.accessioned | 2020-12-17T22:45:18Z | |
dc.date.available | 2020-12-17T22:45:18Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0040-2176 | |
dc.identifier.uri | http://aspace.agrif.bg.ac.rs/handle/123456789/5187 | |
dc.description.abstract | The present study provides insight into changes in the liposomal membrane fluidity as a result of variation of structural components (two types of standard phospholipids - 1,2-dipalmitoyl-sn-glycerero3-phosphocholine, i.e. DPPC and 1-palmitoyl-2-oleoylglycerol-3-phosphocholine, i.e. POPC, one type of phospholipid mixture - Lipoid H and sterols - cholesterol and b-sitosterol), as well as variation in sterol concentration (5-50%). Fluorescence spectroscopy (anisotropy, r) has shown that DPPC liposomal membranes were more rigid compared to POPC and Lipoid H lipid bilayers. Furthermore, the increment of sterol concentration has caused a decrease in the membrane fluidity in all liposomal samples. On the other hand, phospholipid bilayers containing b-sitosterol has possessed better fluidity in comparison to their cholesterol parallels. Higher permeability of b-sitosterol liposomal membranes, as well as the beneficial effects of phytosterol on human health, favor the use of b-sitosterol in the preparation of liposomes as components of food, pharmaceutical and cosmetic products. | en |
dc.description.abstract | Prikazana studija daje uvid u promene fluidnosti lipozomalne membrane koje nastaju kao posledica promene strukturnih komponenata lipozoma (dve vrste standardnih fosfolipida - 1,2-dipalmitoil-snglicero-3-fosfoholin, odnosno DPPC i 1-palmitoil-2-oleoil-sn-glicero-3-fosfoholin, odnosno POPC, jedne vrste komercijalne fosfolipidne smeše - Lipoid H i sterola - holesterol i b-sitosterol), kao i promene udela sterola (0-50 mol %). Fluorimetrijska spektrofotometrija (anizotropija, r) pokazala je da su DPPC lipozomalne membrane bile rigidnije u poređenju sa POPC i Lipoid H lipidnim dvoslojima. Pored toga, povećenje koncentracije sterola prouzrokovalo je smanjenje permeabilnosti membrane u svim lipozomima. Sa druge strane, fosfolipidni dvosloji koji su sadržali b-sitosterol pokazali su veću fluidnost u poređenju sa njihovim paralelama sa holesterolom. Bolja permeabilnost b-sitosterol lipozomalnih membrana, kao i blagotvorni uticaji fitosterola na zdravlje ljudi favorizuju primenu b-sitosterola u pripremi lipozoma kao sastavnih delova prehrambenih, farmaceutskih i kozmetičkih proizvoda. | sr |
dc.publisher | Savez inženjera i tehničara Srbije, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Tehnika | |
dc.subject | b-sitosterol | en |
dc.subject | liposomes | en |
dc.subject | permeability | en |
dc.subject | fluorescence spectroscopy | en |
dc.subject | cholesterol | en |
dc.subject | b-sitosterol | sr |
dc.subject | lipozomi | sr |
dc.subject | permeabilnost | sr |
dc.subject | fluorimetrijska spektrofotometrija | sr |
dc.subject | holesterol | sr |
dc.title | Fluorescence analysis of liposomal membranes permeability | en |
dc.title | Fluorimetrijska analiza permeabilnosti lipozomalnih membrana | sr |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.epage | 498 | |
dc.citation.issue | 4 | |
dc.citation.other | 74(4): 493-498 | |
dc.citation.rank | M51 | |
dc.citation.spage | 493 | |
dc.citation.volume | 74 | |
dc.identifier.doi | 10.5937/tehnika1904493J | |
dc.identifier.fulltext | http://aspace.agrif.bg.ac.rs/bitstream/id/3683/5184.pdf | |
dc.type.version | publishedVersion |