Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle
2019
Autori
Maletić, MilanPaprikić, Nevres
Lazarević, Miodrag
Hodzić, Aida
Davidović, Vesna
Stanisić, Ljubodrag
Stanimirović, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) i...n milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.
Ključne reči:
Busha cattle / leptin polymorphsm / milk traits / genetic markersIzvor:
Acta Veterinaria-Beograd, 2019, 69, 2, 153-163Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2478/acve-2019-0012
ISSN: 0567-8315
WoS: 000472614800002
Scopus: 2-s2.0-85068372915
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Maletić, Milan AU - Paprikić, Nevres AU - Lazarević, Miodrag AU - Hodzić, Aida AU - Davidović, Vesna AU - Stanisić, Ljubodrag AU - Stanimirović, Zoran PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5107 AB - Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle EP - 163 IS - 2 SP - 153 VL - 69 DO - 10.2478/acve-2019-0012 ER -
@article{ author = "Maletić, Milan and Paprikić, Nevres and Lazarević, Miodrag and Hodzić, Aida and Davidović, Vesna and Stanisić, Ljubodrag and Stanimirović, Zoran", year = "2019", abstract = "Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle", pages = "163-153", number = "2", volume = "69", doi = "10.2478/acve-2019-0012" }
Maletić, M., Paprikić, N., Lazarević, M., Hodzić, A., Davidović, V., Stanisić, L.,& Stanimirović, Z.. (2019). Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 153-163. https://doi.org/10.2478/acve-2019-0012
Maletić M, Paprikić N, Lazarević M, Hodzić A, Davidović V, Stanisić L, Stanimirović Z. Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd. 2019;69(2):153-163. doi:10.2478/acve-2019-0012 .
Maletić, Milan, Paprikić, Nevres, Lazarević, Miodrag, Hodzić, Aida, Davidović, Vesna, Stanisić, Ljubodrag, Stanimirović, Zoran, "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle" in Acta Veterinaria-Beograd, 69, no. 2 (2019):153-163, https://doi.org/10.2478/acve-2019-0012 . .