Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation
Samo za registrovane korisnike
2019
Autori
Hassan, Nurul AmirahSupramani, Sugenendran
Sohedein, Mohamad Nor Azzimi
Usuldin, Siti Rokhiyah Ahmad
Klaus, Anita
Ilham, Zul
Chen, Wei-Hsin
Wan-Mohtar, Wan Abd Al Qadr Imad
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p lt 0.05). Temperature and glucose concentration were found to sign...ificantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.
Ključne reči:
Wild-Serbian Ganoderma lucidum / Fungal identification / Exopolysaccharide / Biomass / RSM / Submerged fermentationIzvor:
Biocatalysis and Agricultural Biotechnology, 2019, 21Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- University of Malaya under Ministry of Education Malaysia (Department of Higher Education) [RU003I-2017]
- University of Malaya under Fundamental Research Grant Scheme [FRGS: FP066-2018A]
DOI: 10.1016/j.bcab.2019.101305
ISSN: 1878-8181
WoS: 000500318900029
Scopus: 2-s2.0-85071639793
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Hassan, Nurul Amirah AU - Supramani, Sugenendran AU - Sohedein, Mohamad Nor Azzimi AU - Usuldin, Siti Rokhiyah Ahmad AU - Klaus, Anita AU - Ilham, Zul AU - Chen, Wei-Hsin AU - Wan-Mohtar, Wan Abd Al Qadr Imad PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5007 AB - A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p lt 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains. PB - Elsevier, Amsterdam T2 - Biocatalysis and Agricultural Biotechnology T1 - Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation VL - 21 DO - 10.1016/j.bcab.2019.101305 ER -
@article{ author = "Hassan, Nurul Amirah and Supramani, Sugenendran and Sohedein, Mohamad Nor Azzimi and Usuldin, Siti Rokhiyah Ahmad and Klaus, Anita and Ilham, Zul and Chen, Wei-Hsin and Wan-Mohtar, Wan Abd Al Qadr Imad", year = "2019", abstract = "A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p lt 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.", publisher = "Elsevier, Amsterdam", journal = "Biocatalysis and Agricultural Biotechnology", title = "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation", volume = "21", doi = "10.1016/j.bcab.2019.101305" }
Hassan, N. A., Supramani, S., Sohedein, M. N. A., Usuldin, S. R. A., Klaus, A., Ilham, Z., Chen, W.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology Elsevier, Amsterdam., 21. https://doi.org/10.1016/j.bcab.2019.101305
Hassan NA, Supramani S, Sohedein MNA, Usuldin SRA, Klaus A, Ilham Z, Chen W, Wan-Mohtar WAAQI. Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology. 2019;21. doi:10.1016/j.bcab.2019.101305 .
Hassan, Nurul Amirah, Supramani, Sugenendran, Sohedein, Mohamad Nor Azzimi, Usuldin, Siti Rokhiyah Ahmad, Klaus, Anita, Ilham, Zul, Chen, Wei-Hsin, Wan-Mohtar, Wan Abd Al Qadr Imad, "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation" in Biocatalysis and Agricultural Biotechnology, 21 (2019), https://doi.org/10.1016/j.bcab.2019.101305 . .