HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
2019
Authors
Stanković, JovanaNovaković, Miroslav
Tešević, Vele
Ćirić, Ana
Soković, Marina
Zdunić, Gordana
Dajić-Stevanović, Zora
Godjevac, Dejan
Article (Published version)
Metadata
Show full item recordAbstract
This study was performed to determine the main antibacterial compounds of the essential oil (EO) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC-MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, resp...ectively, at 1/2 MIC concentrations.
Keywords:
antimicrobial activity / direct bioautography / antibiofilm / antiquorum potentialSource:
JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 2019, 84, 12, 1355-1365Publisher:
- Srpsko hemijsko društvo, Beograd
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- Morphological, chemical, pharmacological and agronomic characterization of the Pannonian Thyme (Thymus pannonicus All., Lamiaceae), with the purpose of sustainable production in intensive cropping system (RS-MESTD-Technological Development (TD or TR)-31089)
DOI: 10.2298/JSC190513106S
ISSN: 0352-5139
WoS: 000506674800002
Scopus: 2-s2.0-85079538300
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Poljoprivredni fakultetTY - JOUR AU - Stanković, Jovana AU - Novaković, Miroslav AU - Tešević, Vele AU - Ćirić, Ana AU - Soković, Marina AU - Zdunić, Gordana AU - Dajić-Stevanović, Zora AU - Godjevac, Dejan PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4980 AB - This study was performed to determine the main antibacterial compounds of the essential oil (EO) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC-MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations. PB - Srpsko hemijsko društvo, Beograd T2 - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY T1 - HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil EP - 1365 IS - 12 SP - 1355 VL - 84 DO - 10.2298/JSC190513106S ER -
@article{ author = "Stanković, Jovana and Novaković, Miroslav and Tešević, Vele and Ćirić, Ana and Soković, Marina and Zdunić, Gordana and Dajić-Stevanović, Zora and Godjevac, Dejan", year = "2019", abstract = "This study was performed to determine the main antibacterial compounds of the essential oil (EO) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC-MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY", title = "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil", pages = "1365-1355", number = "12", volume = "84", doi = "10.2298/JSC190513106S" }
Stanković, J., Novaković, M., Tešević, V., Ćirić, A., Soković, M., Zdunić, G., Dajić-Stevanović, Z.,& Godjevac, D.. (2019). HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY Srpsko hemijsko društvo, Beograd., 84(12), 1355-1365. https://doi.org/10.2298/JSC190513106S
Stanković J, Novaković M, Tešević V, Ćirić A, Soković M, Zdunić G, Dajić-Stevanović Z, Godjevac D. HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2019;84(12):1355-1365. doi:10.2298/JSC190513106S .
Stanković, Jovana, Novaković, Miroslav, Tešević, Vele, Ćirić, Ana, Soković, Marina, Zdunić, Gordana, Dajić-Stevanović, Zora, Godjevac, Dejan, "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 84, no. 12 (2019):1355-1365, https://doi.org/10.2298/JSC190513106S . .