Morphological and molecular identification of Eutypa lata on grapevine in Serbia
Само за регистроване кориснике
2019
Аутори
Zivković, SanjaVasić, Tanja
Ivanović, Milan
Jevremović, Darko
Marković, Jordan
Trkulja, Vojislav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions.... Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.
Кључне речи:
Vitis vinifera L / Eutypa lata / Dieback / PCRИзвор:
Journal of Plant Diseases and Protection, 2019, 126, 5, 479-483Издавач:
- Springer Heidelberg, Heidelberg
DOI: 10.1007/s41348-019-00238-4
ISSN: 1861-3829
WoS: 000491548600014
Scopus: 2-s2.0-85068128514
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Zivković, Sanja AU - Vasić, Tanja AU - Ivanović, Milan AU - Jevremović, Darko AU - Marković, Jordan AU - Trkulja, Vojislav PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4958 AB - During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata. PB - Springer Heidelberg, Heidelberg T2 - Journal of Plant Diseases and Protection T1 - Morphological and molecular identification of Eutypa lata on grapevine in Serbia EP - 483 IS - 5 SP - 479 VL - 126 DO - 10.1007/s41348-019-00238-4 ER -
@article{ author = "Zivković, Sanja and Vasić, Tanja and Ivanović, Milan and Jevremović, Darko and Marković, Jordan and Trkulja, Vojislav", year = "2019", abstract = "During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.", publisher = "Springer Heidelberg, Heidelberg", journal = "Journal of Plant Diseases and Protection", title = "Morphological and molecular identification of Eutypa lata on grapevine in Serbia", pages = "483-479", number = "5", volume = "126", doi = "10.1007/s41348-019-00238-4" }
Zivković, S., Vasić, T., Ivanović, M., Jevremović, D., Marković, J.,& Trkulja, V.. (2019). Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection Springer Heidelberg, Heidelberg., 126(5), 479-483. https://doi.org/10.1007/s41348-019-00238-4
Zivković S, Vasić T, Ivanović M, Jevremović D, Marković J, Trkulja V. Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection. 2019;126(5):479-483. doi:10.1007/s41348-019-00238-4 .
Zivković, Sanja, Vasić, Tanja, Ivanović, Milan, Jevremović, Darko, Marković, Jordan, Trkulja, Vojislav, "Morphological and molecular identification of Eutypa lata on grapevine in Serbia" in Journal of Plant Diseases and Protection, 126, no. 5 (2019):479-483, https://doi.org/10.1007/s41348-019-00238-4 . .