Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification
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2019
Authors
Bjelogrlić, SnežanaTodorović, Tamara R.
Kojić, Milan
Sencanski, Milan
Nikolić, Milan
Visnjevac, Aleksandar
Araskov, Jovana
Miljković, Marija
Muller, Christian D.
Filipović, Nenad
Article (Published version)
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Anticancer activity of Pd complexes 1-5 with bidentate N-heteroaromatic hydrazone ligands was investigated on human acute monocytic leukemia (THP-1; cells in a suspension) and human mammary adenocarcinoma (MCF-7; two-dimensional layer and three-dimensional spheroid tumor model) cell lines. For the Pd(II) complexes with condensation products of ethyl hydrazainoacetate and quinoline-8-carboxaldehyde (complex 1) and 2-for-mylpyridine (complex 3), for which apoptosis was determined as a mechanism of anticancer activity, further investigation revealed that they arrest the cell cycle in G0/G1 phase, induce generation of reactive oxygen species and inhibit Topoisomerase I in vitro. In silico studies corroborate experimental findings that these complexes show topoisomerase inhibition activity in the micromolar range and indicate binding to a DNA's minor groove as another potential target. Based on the results obtained by circular dichroism and fluorescence spectroscopy measurements, the most a...ctive complexes are suitable to be delivered to a blood stream via human serum albumin.
Keywords:
Pd(II) complexes / Hydrazones / Apoptosis / Topoisomerase I inhibition / DNA interactions / HSA interactionsSource:
Journal of Inorganic Biochemistry, 2019, 199Publisher:
- Elsevier Science Inc, New York
Funding / projects:
- Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids (RS-172055)
- COSTEuropean Cooperation in Science and Technology (COST) [CA15135, CA16119]
DOI: 10.1016/j.jinorgbio.2019.110758
ISSN: 0162-0134
PubMed: 31299379
WoS: 000488146900009
Scopus: 2-s2.0-85068530825
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Poljoprivredni fakultetTY - JOUR AU - Bjelogrlić, Snežana AU - Todorović, Tamara R. AU - Kojić, Milan AU - Sencanski, Milan AU - Nikolić, Milan AU - Visnjevac, Aleksandar AU - Araskov, Jovana AU - Miljković, Marija AU - Muller, Christian D. AU - Filipović, Nenad PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4916 AB - Anticancer activity of Pd complexes 1-5 with bidentate N-heteroaromatic hydrazone ligands was investigated on human acute monocytic leukemia (THP-1; cells in a suspension) and human mammary adenocarcinoma (MCF-7; two-dimensional layer and three-dimensional spheroid tumor model) cell lines. For the Pd(II) complexes with condensation products of ethyl hydrazainoacetate and quinoline-8-carboxaldehyde (complex 1) and 2-for-mylpyridine (complex 3), for which apoptosis was determined as a mechanism of anticancer activity, further investigation revealed that they arrest the cell cycle in G0/G1 phase, induce generation of reactive oxygen species and inhibit Topoisomerase I in vitro. In silico studies corroborate experimental findings that these complexes show topoisomerase inhibition activity in the micromolar range and indicate binding to a DNA's minor groove as another potential target. Based on the results obtained by circular dichroism and fluorescence spectroscopy measurements, the most active complexes are suitable to be delivered to a blood stream via human serum albumin. PB - Elsevier Science Inc, New York T2 - Journal of Inorganic Biochemistry T1 - Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification VL - 199 DO - 10.1016/j.jinorgbio.2019.110758 ER -
@article{ author = "Bjelogrlić, Snežana and Todorović, Tamara R. and Kojić, Milan and Sencanski, Milan and Nikolić, Milan and Visnjevac, Aleksandar and Araskov, Jovana and Miljković, Marija and Muller, Christian D. and Filipović, Nenad", year = "2019", abstract = "Anticancer activity of Pd complexes 1-5 with bidentate N-heteroaromatic hydrazone ligands was investigated on human acute monocytic leukemia (THP-1; cells in a suspension) and human mammary adenocarcinoma (MCF-7; two-dimensional layer and three-dimensional spheroid tumor model) cell lines. For the Pd(II) complexes with condensation products of ethyl hydrazainoacetate and quinoline-8-carboxaldehyde (complex 1) and 2-for-mylpyridine (complex 3), for which apoptosis was determined as a mechanism of anticancer activity, further investigation revealed that they arrest the cell cycle in G0/G1 phase, induce generation of reactive oxygen species and inhibit Topoisomerase I in vitro. In silico studies corroborate experimental findings that these complexes show topoisomerase inhibition activity in the micromolar range and indicate binding to a DNA's minor groove as another potential target. Based on the results obtained by circular dichroism and fluorescence spectroscopy measurements, the most active complexes are suitable to be delivered to a blood stream via human serum albumin.", publisher = "Elsevier Science Inc, New York", journal = "Journal of Inorganic Biochemistry", title = "Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification", volume = "199", doi = "10.1016/j.jinorgbio.2019.110758" }
Bjelogrlić, S., Todorović, T. R., Kojić, M., Sencanski, M., Nikolić, M., Visnjevac, A., Araskov, J., Miljković, M., Muller, C. D.,& Filipović, N.. (2019). Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification. in Journal of Inorganic Biochemistry Elsevier Science Inc, New York., 199. https://doi.org/10.1016/j.jinorgbio.2019.110758
Bjelogrlić S, Todorović TR, Kojić M, Sencanski M, Nikolić M, Visnjevac A, Araskov J, Miljković M, Muller CD, Filipović N. Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification. in Journal of Inorganic Biochemistry. 2019;199. doi:10.1016/j.jinorgbio.2019.110758 .
Bjelogrlić, Snežana, Todorović, Tamara R., Kojić, Milan, Sencanski, Milan, Nikolić, Milan, Visnjevac, Aleksandar, Araskov, Jovana, Miljković, Marija, Muller, Christian D., Filipović, Nenad, "Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification" in Journal of Inorganic Biochemistry, 199 (2019), https://doi.org/10.1016/j.jinorgbio.2019.110758 . .