Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia
Identifikacija virusa infektivnih za običnu tikvu (Cucurbita pepo L.) u Jugoslaviji
2002
Autori
Krstić, BrankaBerenji, Janoš
Dukić, Nataša
Vico, Ivana
Katis, Nikolaos I.
Papavassiliou, Chryssa
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepo)grown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1). Virus-infected plants showed different symptoms (Table 2 and Figures 1-4). Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 1 (WMV-1), watermelon mosaic potyvirus 2 (WMV-2) and squash mosaic comovirus (SqMV) were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4). The most prevalent viruses inf...ecting pumpkins were ZYMV (62%) and CMV (58%). WMV-2 was extremely rare.
Cilj ovih istraživanja bio je da se identifikuju najvažniji virusi tikava (Cucurbita pepo L) gajenih u Srbiji. Uzorci biljnog materijala uljane tikve-golice, uljane tikve sa ljuskom, tikvice za jelo, patisona i cukinija koji su bili zaraženi virusima, sakupljeni su u tri lokaliteta sredinom jula 2001. godine (tab. 1). Biljke zaražene virusima pokazivale su različite simptome (tab. 2 i sl. 1-4). Tačna determinacija virusa samo na osnovu simptoma nije moguća zbog varijabilnosti samih simptoma. Zaraženi uzorci su testirani biotestom kao i primenom dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antitela na Cucumber mosaic cucomovirus (CMV) Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus 2 (WMV-2), Watermelon mosaic potyvirus 1 (WMV-1) i Squash mosaic comovirus (SqMV). U 50 ispitanih uzoraka detektovan je jedan ili dva virusa (tab. 3 i 4). Preovlađujući virusi tikava bili su ZYMV (62%) i CMV (58%). WMV-2 je detektovan u veoma malom broju uzoraka.
Ključne reči:
Cucurbita pepo / pumpkins / plant viruses / zucchini yellow mosaic potyvirus / cucumber mosaic cucumovirus / watermelon mosaic potyvirus 1 / watermelon mosaic potyvirus 2 / biotest / ELISA / EBIAIzvor:
Zbornik Matice srpske za prirodne nauke, 2002, 103, 67-79Izdavač:
- Matica srpska, Novi Sad
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Krstić, Branka AU - Berenji, Janoš AU - Dukić, Nataša AU - Vico, Ivana AU - Katis, Nikolaos I. AU - Papavassiliou, Chryssa PY - 2002 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/468 AB - This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepo)grown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1). Virus-infected plants showed different symptoms (Table 2 and Figures 1-4). Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 1 (WMV-1), watermelon mosaic potyvirus 2 (WMV-2) and squash mosaic comovirus (SqMV) were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4). The most prevalent viruses infecting pumpkins were ZYMV (62%) and CMV (58%). WMV-2 was extremely rare. AB - Cilj ovih istraživanja bio je da se identifikuju najvažniji virusi tikava (Cucurbita pepo L) gajenih u Srbiji. Uzorci biljnog materijala uljane tikve-golice, uljane tikve sa ljuskom, tikvice za jelo, patisona i cukinija koji su bili zaraženi virusima, sakupljeni su u tri lokaliteta sredinom jula 2001. godine (tab. 1). Biljke zaražene virusima pokazivale su različite simptome (tab. 2 i sl. 1-4). Tačna determinacija virusa samo na osnovu simptoma nije moguća zbog varijabilnosti samih simptoma. Zaraženi uzorci su testirani biotestom kao i primenom dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antitela na Cucumber mosaic cucomovirus (CMV) Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus 2 (WMV-2), Watermelon mosaic potyvirus 1 (WMV-1) i Squash mosaic comovirus (SqMV). U 50 ispitanih uzoraka detektovan je jedan ili dva virusa (tab. 3 i 4). Preovlađujući virusi tikava bili su ZYMV (62%) i CMV (58%). WMV-2 je detektovan u veoma malom broju uzoraka. PB - Matica srpska, Novi Sad T2 - Zbornik Matice srpske za prirodne nauke T1 - Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia T1 - Identifikacija virusa infektivnih za običnu tikvu (Cucurbita pepo L.) u Jugoslaviji EP - 79 IS - 103 SP - 67 UR - https://hdl.handle.net/21.15107/rcub_agrospace_468 ER -
@article{ author = "Krstić, Branka and Berenji, Janoš and Dukić, Nataša and Vico, Ivana and Katis, Nikolaos I. and Papavassiliou, Chryssa", year = "2002", abstract = "This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepo)grown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1). Virus-infected plants showed different symptoms (Table 2 and Figures 1-4). Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 1 (WMV-1), watermelon mosaic potyvirus 2 (WMV-2) and squash mosaic comovirus (SqMV) were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4). The most prevalent viruses infecting pumpkins were ZYMV (62%) and CMV (58%). WMV-2 was extremely rare., Cilj ovih istraživanja bio je da se identifikuju najvažniji virusi tikava (Cucurbita pepo L) gajenih u Srbiji. Uzorci biljnog materijala uljane tikve-golice, uljane tikve sa ljuskom, tikvice za jelo, patisona i cukinija koji su bili zaraženi virusima, sakupljeni su u tri lokaliteta sredinom jula 2001. godine (tab. 1). Biljke zaražene virusima pokazivale su različite simptome (tab. 2 i sl. 1-4). Tačna determinacija virusa samo na osnovu simptoma nije moguća zbog varijabilnosti samih simptoma. Zaraženi uzorci su testirani biotestom kao i primenom dve serološke metode, ELISA i EBIA korišćenjem poliklonalnih antitela na Cucumber mosaic cucomovirus (CMV) Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus 2 (WMV-2), Watermelon mosaic potyvirus 1 (WMV-1) i Squash mosaic comovirus (SqMV). U 50 ispitanih uzoraka detektovan je jedan ili dva virusa (tab. 3 i 4). Preovlađujući virusi tikava bili su ZYMV (62%) i CMV (58%). WMV-2 je detektovan u veoma malom broju uzoraka.", publisher = "Matica srpska, Novi Sad", journal = "Zbornik Matice srpske za prirodne nauke", title = "Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia, Identifikacija virusa infektivnih za običnu tikvu (Cucurbita pepo L.) u Jugoslaviji", pages = "79-67", number = "103", url = "https://hdl.handle.net/21.15107/rcub_agrospace_468" }
Krstić, B., Berenji, J., Dukić, N., Vico, I., Katis, N. I.,& Papavassiliou, C.. (2002). Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia. in Zbornik Matice srpske za prirodne nauke Matica srpska, Novi Sad.(103), 67-79. https://hdl.handle.net/21.15107/rcub_agrospace_468
Krstić B, Berenji J, Dukić N, Vico I, Katis NI, Papavassiliou C. Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia. in Zbornik Matice srpske za prirodne nauke. 2002;(103):67-79. https://hdl.handle.net/21.15107/rcub_agrospace_468 .
Krstić, Branka, Berenji, Janoš, Dukić, Nataša, Vico, Ivana, Katis, Nikolaos I., Papavassiliou, Chryssa, "Identification of viruses infecting pumpkins (Cucurbita pepo L.) in Yugoslavia" in Zbornik Matice srpske za prirodne nauke, no. 103 (2002):67-79, https://hdl.handle.net/21.15107/rcub_agrospace_468 .