Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs
Само за регистроване кориснике
2018
Аутори
Vranković, JelenaZivić, Miroslav
Radojević, Andelina
Perić-Mataruga, Vesna
Todorović, Dajana
Marković, Zoran
Živić, Ivana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The current study reports a combined seasonal monitoring of the Crnica River watercourse using physicochemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Ou...r findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream.
Кључне речи:
Trout farm / Gammarus dulensis / Antioxidant enzyme / Trace elements / SeasonИзвор:
Ecotoxicology and Environmental Safety, 2018, 163, 84-95Издавач:
- Academic Press Inc Elsevier Science, San Diego
Финансирање / пројекти:
- Унапређење производних капацитета шарана (Cyprinus carpio L) програмима исхране и селекције (RS-31075)
DOI: 10.1016/j.ecoenv.2018.07.061
ISSN: 0147-6513
PubMed: 30041129
WoS: 000443104400011
Scopus: 2-s2.0-85050109145
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Vranković, Jelena AU - Zivić, Miroslav AU - Radojević, Andelina AU - Perić-Mataruga, Vesna AU - Todorović, Dajana AU - Marković, Zoran AU - Živić, Ivana PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4663 AB - The current study reports a combined seasonal monitoring of the Crnica River watercourse using physicochemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Our findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream. PB - Academic Press Inc Elsevier Science, San Diego T2 - Ecotoxicology and Environmental Safety T1 - Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs EP - 95 SP - 84 VL - 163 DO - 10.1016/j.ecoenv.2018.07.061 ER -
@article{ author = "Vranković, Jelena and Zivić, Miroslav and Radojević, Andelina and Perić-Mataruga, Vesna and Todorović, Dajana and Marković, Zoran and Živić, Ivana", year = "2018", abstract = "The current study reports a combined seasonal monitoring of the Crnica River watercourse using physicochemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Our findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Ecotoxicology and Environmental Safety", title = "Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs", pages = "95-84", volume = "163", doi = "10.1016/j.ecoenv.2018.07.061" }
Vranković, J., Zivić, M., Radojević, A., Perić-Mataruga, V., Todorović, D., Marković, Z.,& Živić, I.. (2018). Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs. in Ecotoxicology and Environmental Safety Academic Press Inc Elsevier Science, San Diego., 163, 84-95. https://doi.org/10.1016/j.ecoenv.2018.07.061
Vranković J, Zivić M, Radojević A, Perić-Mataruga V, Todorović D, Marković Z, Živić I. Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs. in Ecotoxicology and Environmental Safety. 2018;163:84-95. doi:10.1016/j.ecoenv.2018.07.061 .
Vranković, Jelena, Zivić, Miroslav, Radojević, Andelina, Perić-Mataruga, Vesna, Todorović, Dajana, Marković, Zoran, Živić, Ivana, "Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs" in Ecotoxicology and Environmental Safety, 163 (2018):84-95, https://doi.org/10.1016/j.ecoenv.2018.07.061 . .