Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
Само за регистроване кориснике
2017
Аутори
Bosnjak-Neumuller, JasnaRadaković, Milena
Djelić, Ninoslav
Vuković-Gačić, Branka
Dajić-Stevanović, Zora
Kolarević, Stoimir
Misić, Danijela
Stanković, Milan
Knežević-Vukčević, Jelena
Spremo-Potparević, Biljana
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro usin...g the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).
Кључне речи:
triiodothyronine / DNA damage / Nepeta rtanjensis / phenolic compounds and antigenotoxic activityИзвор:
Pakistan journal of pharmaceutical sciences, 2017, 30, 2, 625-634Издавач:
- NLM (Medline)
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Bosnjak-Neumuller, Jasna AU - Radaković, Milena AU - Djelić, Ninoslav AU - Vuković-Gačić, Branka AU - Dajić-Stevanović, Zora AU - Kolarević, Stoimir AU - Misić, Danijela AU - Stanković, Milan AU - Knežević-Vukčević, Jelena AU - Spremo-Potparević, Biljana AU - Stanimirović, Zoran PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4504 AB - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS). PB - NLM (Medline) T2 - Pakistan journal of pharmaceutical sciences T1 - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes EP - 634 IS - 2 SP - 625 VL - 30 UR - https://hdl.handle.net/21.15107/rcub_agrospace_4504 ER -
@article{ author = "Bosnjak-Neumuller, Jasna and Radaković, Milena and Djelić, Ninoslav and Vuković-Gačić, Branka and Dajić-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knežević-Vukčević, Jelena and Spremo-Potparević, Biljana and Stanimirović, Zoran", year = "2017", abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).", publisher = "NLM (Medline)", journal = "Pakistan journal of pharmaceutical sciences", title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes", pages = "634-625", number = "2", volume = "30", url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504" }
Bosnjak-Neumuller, J., Radaković, M., Djelić, N., Vuković-Gačić, B., Dajić-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knežević-Vukčević, J., Spremo-Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences NLM (Medline)., 30(2), 625-634. https://hdl.handle.net/21.15107/rcub_agrospace_4504
Bosnjak-Neumuller J, Radaković M, Djelić N, Vuković-Gačić B, Dajić-Stevanović Z, Kolarević S, Misić D, Stanković M, Knežević-Vukčević J, Spremo-Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences. 2017;30(2):625-634. https://hdl.handle.net/21.15107/rcub_agrospace_4504 .
Bosnjak-Neumuller, Jasna, Radaković, Milena, Djelić, Ninoslav, Vuković-Gačić, Branka, Dajić-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knežević-Vukčević, Jelena, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan journal of pharmaceutical sciences, 30, no. 2 (2017):625-634, https://hdl.handle.net/21.15107/rcub_agrospace_4504 .