Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines
Abstract
Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) show...ed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.
Keywords:
Wheat / NILs / glutenins / PDIs / qRT-PCRSource:
Genetika, 2016, 48, 1, 249-269Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
- National Natural Science Foundation of China (NSFC) [31471485]
- Natural Science Foundation of Beijing CityBeijing Natural Science Foundation
- Key Developmental Project of Science and Technology from Beijing Municipal Commission of Education [KZ201410028031]
- China-Australia Cooperation Project from Chinese Ministry of Science and Technology [2013DFG30530]
DOI: 10.2298/GENSR1601249D
ISSN: 0534-0012
WoS: 000376744800021
Scopus: 2-s2.0-84971283680
Collections
Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Dong, Liwei AU - Li, Ning AU - Lu, Xiaobing AU - Prodanović, Slaven AU - Xu, Yanhao AU - Zhang, Wenying AU - Yan, Yueming PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4191 AB - Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines EP - 269 IS - 1 SP - 249 VL - 48 DO - 10.2298/GENSR1601249D ER -
@article{ author = "Dong, Liwei and Li, Ning and Lu, Xiaobing and Prodanović, Slaven and Xu, Yanhao and Zhang, Wenying and Yan, Yueming", year = "2016", abstract = "Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines", pages = "269-249", number = "1", volume = "48", doi = "10.2298/GENSR1601249D" }
Dong, L., Li, N., Lu, X., Prodanović, S., Xu, Y., Zhang, W.,& Yan, Y.. (2016). Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika Društvo genetičara Srbije, Beograd., 48(1), 249-269. https://doi.org/10.2298/GENSR1601249D
Dong L, Li N, Lu X, Prodanović S, Xu Y, Zhang W, Yan Y. Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika. 2016;48(1):249-269. doi:10.2298/GENSR1601249D .
Dong, Liwei, Li, Ning, Lu, Xiaobing, Prodanović, Slaven, Xu, Yanhao, Zhang, Wenying, Yan, Yueming, "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines" in Genetika, 48, no. 1 (2016):249-269, https://doi.org/10.2298/GENSR1601249D . .