Towards antimicrobial yet bioactive Cu-alginate hydrogels
Samo za registrovane korisnike
2016
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effec...ts against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.
Ključne reči:
copper / alginate / mechanical property / antibacterial activity / cartilage tissue engineeringIzvor:
Biomedical Materials, 2016, 11, 3Izdavač:
- IOP Publishing Ltd, Bristol
Finansiranje / projekti:
- Sinteza, razvoj tehnologija dobijanja i primena nanostrukturnih multifunkcionalnih materijala definisanih svojstava (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45019)
- Ministry of Education, Science and Technological Development of the Republic of Serbia [Eureka E!6749]
DOI: 10.1088/1748-6041/11/3/035015
ISSN: 1748-6041
PubMed: 27305176
WoS: 000378167700016
Scopus: 2-s2.0-84977645625
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Madzovska-Malagurski, I. AU - Vukašinović-Sekulić, M. AU - Kostić, D. AU - Lević, Steva PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4181 AB - The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants. PB - IOP Publishing Ltd, Bristol T2 - Biomedical Materials T1 - Towards antimicrobial yet bioactive Cu-alginate hydrogels IS - 3 VL - 11 DO - 10.1088/1748-6041/11/3/035015 ER -
@article{ author = "Madzovska-Malagurski, I. and Vukašinović-Sekulić, M. and Kostić, D. and Lević, Steva", year = "2016", abstract = "The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.", publisher = "IOP Publishing Ltd, Bristol", journal = "Biomedical Materials", title = "Towards antimicrobial yet bioactive Cu-alginate hydrogels", number = "3", volume = "11", doi = "10.1088/1748-6041/11/3/035015" }
Madzovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D.,& Lević, S.. (2016). Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials IOP Publishing Ltd, Bristol., 11(3). https://doi.org/10.1088/1748-6041/11/3/035015
Madzovska-Malagurski I, Vukašinović-Sekulić M, Kostić D, Lević S. Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials. 2016;11(3). doi:10.1088/1748-6041/11/3/035015 .
Madzovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D., Lević, Steva, "Towards antimicrobial yet bioactive Cu-alginate hydrogels" in Biomedical Materials, 11, no. 3 (2016), https://doi.org/10.1088/1748-6041/11/3/035015 . .