Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia
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2016
Authors
Ivanović, MilanGašić, K.
Prokić, Andjelka
Kuzmanović, Nemanja
Zlatković, Nevena
Obradović, Aleksa
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Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amend...ed with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.
Keywords:
fire blight / streptomycin / kasugamycin / copper sulfate / control / screeningSource:
III Balkan Symposium on Fruit Growing, 2016, 1139, 715-719Publisher:
- Int Soc Horticultural Science, Leuven 1
Funding / projects:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)
DOI: 10.17660/ActaHortic.2016.1139.122
ISSN: 0567-7572
WoS: 000385238300122
Scopus: 2-s2.0-84991490684
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Poljoprivredni fakultetTY - CONF AU - Ivanović, Milan AU - Gašić, K. AU - Prokić, Andjelka AU - Kuzmanović, Nemanja AU - Zlatković, Nevena AU - Obradović, Aleksa PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4098 AB - Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia. PB - Int Soc Horticultural Science, Leuven 1 C3 - III Balkan Symposium on Fruit Growing T1 - Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia EP - 719 SP - 715 VL - 1139 DO - 10.17660/ActaHortic.2016.1139.122 ER -
@conference{ author = "Ivanović, Milan and Gašić, K. and Prokić, Andjelka and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa", year = "2016", abstract = "Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.", publisher = "Int Soc Horticultural Science, Leuven 1", journal = "III Balkan Symposium on Fruit Growing", title = "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia", pages = "719-715", volume = "1139", doi = "10.17660/ActaHortic.2016.1139.122" }
Ivanović, M., Gašić, K., Prokić, A., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2016). Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing Int Soc Horticultural Science, Leuven 1., 1139, 715-719. https://doi.org/10.17660/ActaHortic.2016.1139.122
Ivanović M, Gašić K, Prokić A, Kuzmanović N, Zlatković N, Obradović A. Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing. 2016;1139:715-719. doi:10.17660/ActaHortic.2016.1139.122 .
Ivanović, Milan, Gašić, K., Prokić, Andjelka, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia" in III Balkan Symposium on Fruit Growing, 1139 (2016):715-719, https://doi.org/10.17660/ActaHortic.2016.1139.122 . .