Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat
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2016
Authors
Vidović, MarijaMorina, Filis
Prokić, Ljiljana
Milić-Komić, Sonja
Zivanović, Bojana
Veljović-Jovanović, Sonja
Article (Published version)
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In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat additio...n stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.
Keywords:
Abscisic acid / Ascorbate-glutathione cycle / Strong light / Hydrogen peroxide / Paraquat / Variegated plantsSource:
Journal of Plant Physiology, 2016, 206, 25-39Publisher:
- Elsevier Gmbh, Munich
Funding / projects:
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
DOI: 10.1016/j.jplph.2016.07.017
ISSN: 0176-1617
PubMed: 27688091
WoS: 000389596500003
Scopus: 2-s2.0-84988719255
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Poljoprivredni fakultetTY - JOUR AU - Vidović, Marija AU - Morina, Filis AU - Prokić, Ljiljana AU - Milić-Komić, Sonja AU - Zivanović, Bojana AU - Veljović-Jovanović, Sonja PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4068 AB - In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed. PB - Elsevier Gmbh, Munich T2 - Journal of Plant Physiology T1 - Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat EP - 39 SP - 25 VL - 206 DO - 10.1016/j.jplph.2016.07.017 ER -
@article{ author = "Vidović, Marija and Morina, Filis and Prokić, Ljiljana and Milić-Komić, Sonja and Zivanović, Bojana and Veljović-Jovanović, Sonja", year = "2016", abstract = "In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100 mu mol m(-2) s(-1) of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H2O2 regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H2O2 in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H2O2 accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H2O2 in signaling were discussed.", publisher = "Elsevier Gmbh, Munich", journal = "Journal of Plant Physiology", title = "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat", pages = "39-25", volume = "206", doi = "10.1016/j.jplph.2016.07.017" }
Vidović, M., Morina, F., Prokić, L., Milić-Komić, S., Zivanović, B.,& Veljović-Jovanović, S.. (2016). Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology Elsevier Gmbh, Munich., 206, 25-39. https://doi.org/10.1016/j.jplph.2016.07.017
Vidović M, Morina F, Prokić L, Milić-Komić S, Zivanović B, Veljović-Jovanović S. Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. in Journal of Plant Physiology. 2016;206:25-39. doi:10.1016/j.jplph.2016.07.017 .
Vidović, Marija, Morina, Filis, Prokić, Ljiljana, Milić-Komić, Sonja, Zivanović, Bojana, Veljović-Jovanović, Sonja, "Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat" in Journal of Plant Physiology, 206 (2016):25-39, https://doi.org/10.1016/j.jplph.2016.07.017 . .