Identification of Rhizoctonia spp. using polyclonal antiserum in ebia

Abstract

A polyclonal antiserum (As-1) raised against total mycelial antigens of binu-cleate Rhizoctonia AG-I was used in EBIA in order to determine antigenic properties and serological relationship of some binucleate and multinucleate Rhizoctonia spp. After separation by electrophoresis and based on the reactions with antiserum As-1, it has been established that a total of 18 protein fractions show antigenic properties (14.4-94kDa). The number and patterns of protein bands differed in binucleate and multinucleate Rhizoctonia isolates and their anastomosis groups (AG). Protein fraction designated as number 8 was common to most of the isolates but the profiles of the remaining bands pointed out serological differences. The results show that the use of polyclonal antiserums can be helpful in detection of different Rhizoctonia spp., but, what is more important, when a polyclonal antiserum is used in EBIA, it is possible to differentiate anastomosis groups of binucleate or multinucleate Rhizoctonia spp.

Antigeni kompleks i serološki međuodnosi odabranih dvojedarnih i višejedarnih izolata Rhizoctonia spp. ispitivani su, primenom EBIA metode, na osnovu reaktivnosti sa antiserumom pripremljenim na ukupne proteine micelije izolata dvojedarne Rhizoctonia AG-I. Elektroforetskim razdvajanjem utvrđeno je da antigena svojstva poseduje ukupno 18 različitih proteinskih komponenti ispitivanih izolata (14,4 - 94 kDa). Ustanovljenje različit broj i raspored proteinskih traka kako kod dvojedarnih i višejedarnih izolata, tako i kod ana-stomoznih grupa (AG). Proteinska frakcija obeležena sa 8 bila je zajednička za najveći broj ispitivanih izolata Rhizoctonia spp., dok je profil ostalih traka ukazao na značajne serološke razlike. Obavljena ispitivanja su pokazala da se primenom poliklonalnog antiseruma mogu detektovati različite Rhizoctonia spp., kao i da se primenom poliklonalnog antiseruma u EBIA metodi mogu identifikovati anastomozne grupe dvojedarnih odnosno višejedarnih Rhizoctonia spp.