Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis
Samo za registrovane korisnike
2015
Autori
Ristivojević, PetarTrifković, Jelena
Gašić, Uroš M.
Andrić, Filip Lj.
Nedić, Nebojša
Tešić, Živoslav
Milojković-Opsenica, Dušanka M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
IntroductionPropolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee-product exhibits numerous biological activities, including anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, immunostimulating and anti-tumour effects. As the chemical composition and biological activity of propolis depend on its botanical and geographical origin, searching for new bioactive substances in various types of propolis from unexplored regions is of great importance. ObjectiveThe aim of this study is the evaluation of the phenolic profile of poplar propolis samples in order to characterise Serbian propolis, to identify possible new constituents and to specify the phenolic components relevant for differentiation of poplar propolis samples into two subgroups through simultaneous analysis of poplar bud extracts. MethodsEthanolic extracts of propolis and poplar buds were comprehensively analysed using ultrahi...gh-performance liquid chromatography coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser together with chemometric methods. ResultsExtensive fingerprint analysis of Serbian propolis was achieved for the first time. Seventy-five phenolic compounds were detected. Eight of them were identified in propolis for the first time. Pattern-recognition methods applied to the content of ten quantified phenolics verified the existence of two subgroups of propolis, with galangin, chrysin and pinocembrin as the most influential distinguishing factors. ConclusionThe phenolic composition of the analysed propolis samples confirm their affiliation to the European poplar type propolis and the existence of two subgroups according to botanical origin.
Ključne reči:
Pattern-recognition methods / phenolic profile / UHPLC-LTQ / Orbitrap / MS / MS / poplar type propolisIzvor:
Phytochemical Analysis, 2015, 26, 2, 127-136Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (RS-MESTD-Basic Research (BR or ON)-172017)
- Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research (EU-FP7-256716)
DOI: 10.1002/pca.2544
ISSN: 0958-0344
PubMed: 25376949
WoS: 000349963400005
Scopus: 2-s2.0-84923096010
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Ristivojević, Petar AU - Trifković, Jelena AU - Gašić, Uroš M. AU - Andrić, Filip Lj. AU - Nedić, Nebojša AU - Tešić, Živoslav AU - Milojković-Opsenica, Dušanka M. PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3815 AB - IntroductionPropolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee-product exhibits numerous biological activities, including anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, immunostimulating and anti-tumour effects. As the chemical composition and biological activity of propolis depend on its botanical and geographical origin, searching for new bioactive substances in various types of propolis from unexplored regions is of great importance. ObjectiveThe aim of this study is the evaluation of the phenolic profile of poplar propolis samples in order to characterise Serbian propolis, to identify possible new constituents and to specify the phenolic components relevant for differentiation of poplar propolis samples into two subgroups through simultaneous analysis of poplar bud extracts. MethodsEthanolic extracts of propolis and poplar buds were comprehensively analysed using ultrahigh-performance liquid chromatography coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser together with chemometric methods. ResultsExtensive fingerprint analysis of Serbian propolis was achieved for the first time. Seventy-five phenolic compounds were detected. Eight of them were identified in propolis for the first time. Pattern-recognition methods applied to the content of ten quantified phenolics verified the existence of two subgroups of propolis, with galangin, chrysin and pinocembrin as the most influential distinguishing factors. ConclusionThe phenolic composition of the analysed propolis samples confirm their affiliation to the European poplar type propolis and the existence of two subgroups according to botanical origin. PB - Wiley, Hoboken T2 - Phytochemical Analysis T1 - Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis EP - 136 IS - 2 SP - 127 VL - 26 DO - 10.1002/pca.2544 ER -
@article{ author = "Ristivojević, Petar and Trifković, Jelena and Gašić, Uroš M. and Andrić, Filip Lj. and Nedić, Nebojša and Tešić, Živoslav and Milojković-Opsenica, Dušanka M.", year = "2015", abstract = "IntroductionPropolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee-product exhibits numerous biological activities, including anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, immunostimulating and anti-tumour effects. As the chemical composition and biological activity of propolis depend on its botanical and geographical origin, searching for new bioactive substances in various types of propolis from unexplored regions is of great importance. ObjectiveThe aim of this study is the evaluation of the phenolic profile of poplar propolis samples in order to characterise Serbian propolis, to identify possible new constituents and to specify the phenolic components relevant for differentiation of poplar propolis samples into two subgroups through simultaneous analysis of poplar bud extracts. MethodsEthanolic extracts of propolis and poplar buds were comprehensively analysed using ultrahigh-performance liquid chromatography coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser together with chemometric methods. ResultsExtensive fingerprint analysis of Serbian propolis was achieved for the first time. Seventy-five phenolic compounds were detected. Eight of them were identified in propolis for the first time. Pattern-recognition methods applied to the content of ten quantified phenolics verified the existence of two subgroups of propolis, with galangin, chrysin and pinocembrin as the most influential distinguishing factors. ConclusionThe phenolic composition of the analysed propolis samples confirm their affiliation to the European poplar type propolis and the existence of two subgroups according to botanical origin.", publisher = "Wiley, Hoboken", journal = "Phytochemical Analysis", title = "Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis", pages = "136-127", number = "2", volume = "26", doi = "10.1002/pca.2544" }
Ristivojević, P., Trifković, J., Gašić, U. M., Andrić, F. Lj., Nedić, N., Tešić, Ž.,& Milojković-Opsenica, D. M.. (2015). Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. in Phytochemical Analysis Wiley, Hoboken., 26(2), 127-136. https://doi.org/10.1002/pca.2544
Ristivojević P, Trifković J, Gašić UM, Andrić FL, Nedić N, Tešić Ž, Milojković-Opsenica DM. Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. in Phytochemical Analysis. 2015;26(2):127-136. doi:10.1002/pca.2544 .
Ristivojević, Petar, Trifković, Jelena, Gašić, Uroš M., Andrić, Filip Lj., Nedić, Nebojša, Tešić, Živoslav, Milojković-Opsenica, Dušanka M., "Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis" in Phytochemical Analysis, 26, no. 2 (2015):127-136, https://doi.org/10.1002/pca.2544 . .