Detecting population admixture in honey bees of Serbia
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2014
Authors
Nedić, NebojšaFrancis, Roy Mathew
Stanisavljević, Ljubiša
Pihler, Ivan
Kezić, Nikola
Bendixen, Christian
Kryger, Per
Article (Published version)
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Honey bee workers were sampled across the Serbian territory during 2009-2010 from mostly non-migratory apiaries to determine the population structure of these bees using morphometric, genetic, and spatial information. A total of 134 bees were sampled, of which 77 were analysed using classical wing morphometrics and 122 bees were successfully analysed using 24 DNA microsatellite markers. A combination of methods including multivariate statistics and assignment tests (frequency-based and Bayesian) revealed the honey bees of this region to resemble the subspecies Apt mellifera macedonica, Apis mellifera carnica or hybrids of these two subspecies. Based on Bayesian assignment ('Structure') and spatial PCA, honey bees within the Serbian territory were composed of 56%-58% A. m. carica and 42%-44% A. m. macedonica. Spatial analysis showed the existence of a north-west to south-east dine in genetic differentiation. The bees in the north-west resemble A. m. carnica, while the bees in the south-...east of the country are more similar to A. m. macedonica. Thus, the extent of A. m. macedonica within Serbia was greater than previously estimated. We define a line of hybridisation between A. m. carnica and A. m. macedonica within our study area. The dine of differentiation was still evident using a combination of genetic and spatial information, in spite of beekeeping activities including transhumance and breeding efforts.
Keywords:
DNA microsatellites / classical wing morphometry / population structure / adegenet / spatial PCA / admixture / Apis mellifera L. / SerbiaSource:
Journal of Apicultural Research, 2014, 53, 2, 303-313Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-46009)
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-43001)
DOI: 10.3896/IBRA.1.53.2.12
ISSN: 0021-8839
WoS: 000337932500013
Scopus: 2-s2.0-84901786930
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Poljoprivredni fakultetTY - JOUR AU - Nedić, Nebojša AU - Francis, Roy Mathew AU - Stanisavljević, Ljubiša AU - Pihler, Ivan AU - Kezić, Nikola AU - Bendixen, Christian AU - Kryger, Per PY - 2014 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3415 AB - Honey bee workers were sampled across the Serbian territory during 2009-2010 from mostly non-migratory apiaries to determine the population structure of these bees using morphometric, genetic, and spatial information. A total of 134 bees were sampled, of which 77 were analysed using classical wing morphometrics and 122 bees were successfully analysed using 24 DNA microsatellite markers. A combination of methods including multivariate statistics and assignment tests (frequency-based and Bayesian) revealed the honey bees of this region to resemble the subspecies Apt mellifera macedonica, Apis mellifera carnica or hybrids of these two subspecies. Based on Bayesian assignment ('Structure') and spatial PCA, honey bees within the Serbian territory were composed of 56%-58% A. m. carica and 42%-44% A. m. macedonica. Spatial analysis showed the existence of a north-west to south-east dine in genetic differentiation. The bees in the north-west resemble A. m. carnica, while the bees in the south-east of the country are more similar to A. m. macedonica. Thus, the extent of A. m. macedonica within Serbia was greater than previously estimated. We define a line of hybridisation between A. m. carnica and A. m. macedonica within our study area. The dine of differentiation was still evident using a combination of genetic and spatial information, in spite of beekeeping activities including transhumance and breeding efforts. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Apicultural Research T1 - Detecting population admixture in honey bees of Serbia EP - 313 IS - 2 SP - 303 VL - 53 DO - 10.3896/IBRA.1.53.2.12 ER -
@article{ author = "Nedić, Nebojša and Francis, Roy Mathew and Stanisavljević, Ljubiša and Pihler, Ivan and Kezić, Nikola and Bendixen, Christian and Kryger, Per", year = "2014", abstract = "Honey bee workers were sampled across the Serbian territory during 2009-2010 from mostly non-migratory apiaries to determine the population structure of these bees using morphometric, genetic, and spatial information. A total of 134 bees were sampled, of which 77 were analysed using classical wing morphometrics and 122 bees were successfully analysed using 24 DNA microsatellite markers. A combination of methods including multivariate statistics and assignment tests (frequency-based and Bayesian) revealed the honey bees of this region to resemble the subspecies Apt mellifera macedonica, Apis mellifera carnica or hybrids of these two subspecies. Based on Bayesian assignment ('Structure') and spatial PCA, honey bees within the Serbian territory were composed of 56%-58% A. m. carica and 42%-44% A. m. macedonica. Spatial analysis showed the existence of a north-west to south-east dine in genetic differentiation. The bees in the north-west resemble A. m. carnica, while the bees in the south-east of the country are more similar to A. m. macedonica. Thus, the extent of A. m. macedonica within Serbia was greater than previously estimated. We define a line of hybridisation between A. m. carnica and A. m. macedonica within our study area. The dine of differentiation was still evident using a combination of genetic and spatial information, in spite of beekeeping activities including transhumance and breeding efforts.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Apicultural Research", title = "Detecting population admixture in honey bees of Serbia", pages = "313-303", number = "2", volume = "53", doi = "10.3896/IBRA.1.53.2.12" }
Nedić, N., Francis, R. M., Stanisavljević, L., Pihler, I., Kezić, N., Bendixen, C.,& Kryger, P.. (2014). Detecting population admixture in honey bees of Serbia. in Journal of Apicultural Research Taylor & Francis Ltd, Abingdon., 53(2), 303-313. https://doi.org/10.3896/IBRA.1.53.2.12
Nedić N, Francis RM, Stanisavljević L, Pihler I, Kezić N, Bendixen C, Kryger P. Detecting population admixture in honey bees of Serbia. in Journal of Apicultural Research. 2014;53(2):303-313. doi:10.3896/IBRA.1.53.2.12 .
Nedić, Nebojša, Francis, Roy Mathew, Stanisavljević, Ljubiša, Pihler, Ivan, Kezić, Nikola, Bendixen, Christian, Kryger, Per, "Detecting population admixture in honey bees of Serbia" in Journal of Apicultural Research, 53, no. 2 (2014):303-313, https://doi.org/10.3896/IBRA.1.53.2.12 . .