Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers
2013
Authors
Ahsyee, Salem R.Al-Sloge, O.
Ćalić, Irena
Branković, Gordana
Zorić, M.
Momirović, Una
Vasiljević, Sanja
Šurlan-Momirović, Gordana
Article (Published version)
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Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.
Keywords:
Alfalfa / genetic diversity / RAPDSource:
Archives of Biological Sciences, 2013, 65, 2, 595-602Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
- Department of Genetics of Biology Research Center in Tripoli
- Increasing the market significance of forage crops by breeding and optimizing seed production technology (RS-31024)
DOI: 10.2298/ABS1302595A
ISSN: 0354-4664
WoS: 000318826000023
Scopus: 2-s2.0-84876956906
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Poljoprivredni fakultetTY - JOUR AU - Ahsyee, Salem R. AU - Al-Sloge, O. AU - Ćalić, Irena AU - Branković, Gordana AU - Zorić, M. AU - Momirović, Una AU - Vasiljević, Sanja AU - Šurlan-Momirović, Gordana PY - 2013 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3169 AB - Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers EP - 602 IS - 2 SP - 595 VL - 65 DO - 10.2298/ABS1302595A ER -
@article{ author = "Ahsyee, Salem R. and Al-Sloge, O. and Ćalić, Irena and Branković, Gordana and Zorić, M. and Momirović, Una and Vasiljević, Sanja and Šurlan-Momirović, Gordana", year = "2013", abstract = "Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers", pages = "602-595", number = "2", volume = "65", doi = "10.2298/ABS1302595A" }
Ahsyee, S. R., Al-Sloge, O., Ćalić, I., Branković, G., Zorić, M., Momirović, U., Vasiljević, S.,& Šurlan-Momirović, G.. (2013). Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 65(2), 595-602. https://doi.org/10.2298/ABS1302595A
Ahsyee SR, Al-Sloge O, Ćalić I, Branković G, Zorić M, Momirović U, Vasiljević S, Šurlan-Momirović G. Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences. 2013;65(2):595-602. doi:10.2298/ABS1302595A .
Ahsyee, Salem R., Al-Sloge, O., Ćalić, Irena, Branković, Gordana, Zorić, M., Momirović, Una, Vasiljević, Sanja, Šurlan-Momirović, Gordana, "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers" in Archives of Biological Sciences, 65, no. 2 (2013):595-602, https://doi.org/10.2298/ABS1302595A . .