Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination
Samo za registrovane korisnike
2012
Autori
Šmigić, NadaRajković, Andreja
Arneborg, Nils
Siegumfeldt, Henrik
Devlieghere, Frank
Nielsen, Dennis Sandris
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.
Ključne reči:
Escherichia coli O157:H7 / Chlorine dioxide / Sublethal injury / Intracellular pHIzvor:
Food Analytical Methods, 2012, 5, 3, 327-331Izdavač:
- Springer, New York
Finansiranje / projekti:
- European CommissionEuropean CommissionEuropean Commission Joint Research Centre [FOOD-CT-2005-007081]
DOI: 10.1007/s12161-011-9295-0
ISSN: 1936-9751
WoS: 000303466800002
Scopus: 2-s2.0-84860474116
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Šmigić, Nada AU - Rajković, Andreja AU - Arneborg, Nils AU - Siegumfeldt, Henrik AU - Devlieghere, Frank AU - Nielsen, Dennis Sandris PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3024 AB - The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased. PB - Springer, New York T2 - Food Analytical Methods T1 - Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination EP - 331 IS - 3 SP - 327 VL - 5 DO - 10.1007/s12161-011-9295-0 ER -
@article{ author = "Šmigić, Nada and Rajković, Andreja and Arneborg, Nils and Siegumfeldt, Henrik and Devlieghere, Frank and Nielsen, Dennis Sandris", year = "2012", abstract = "The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.", publisher = "Springer, New York", journal = "Food Analytical Methods", title = "Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination", pages = "331-327", number = "3", volume = "5", doi = "10.1007/s12161-011-9295-0" }
Šmigić, N., Rajković, A., Arneborg, N., Siegumfeldt, H., Devlieghere, F.,& Nielsen, D. S.. (2012). Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination. in Food Analytical Methods Springer, New York., 5(3), 327-331. https://doi.org/10.1007/s12161-011-9295-0
Šmigić N, Rajković A, Arneborg N, Siegumfeldt H, Devlieghere F, Nielsen DS. Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination. in Food Analytical Methods. 2012;5(3):327-331. doi:10.1007/s12161-011-9295-0 .
Šmigić, Nada, Rajković, Andreja, Arneborg, Nils, Siegumfeldt, Henrik, Devlieghere, Frank, Nielsen, Dennis Sandris, "Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination" in Food Analytical Methods, 5, no. 3 (2012):327-331, https://doi.org/10.1007/s12161-011-9295-0 . .