Mushrooms as food and supplements - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production
Само за регистроване кориснике
2018
Аутори
Miletić, DunjaPantić, Milena
Lević, Steva
Matijašević, Danka
Sknepnek, Aleksandra
Pavlović, Vladimir
Nedović, Viktor
Nikšić, Miomir
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other
activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting
body and mycelia are recognized as potential functional food and dietary supplement. On the
market, there are already many products based on the fungi extracts. For certain types of
mushrooms, submerged production of bioactive compounds stands out as the best method from
the standpoint of industrial applications. Mushrooms are becoming an attractive functional food
and overview about type of food suitable for enrichment with this valuable compounds were
given.
The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate
bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth
(exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential.
During fermentation residual glucose was quan...tified by HPLC. Mycelia products were
characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of
isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative
bacteria strains using broth microdilution method. Results showed that after 48h glucose
concentration started to decrease and after day seven slowed down, when process was
terminated saving energy and time. Applied gentle stirring during cultivation provided constant
oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy
pellets with long, branched and anastomosed hyphae of high density were formed. In prepared
mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex
chemical composition of methanol extracts comparing to EPS was noted. That might be the
explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol
extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample
showed significant activity against both Gram-positive and Gram-negative strains. Based on
obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were
more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising
process for bioactive compounds production.
Кључне речи:
Antibacterial activity / Submerged cultivation / Coriolus versicolor / Exopolysaccharides / MyceliumИзвор:
2nd B-fost 2018 Congress 15-17 October Yerevan, 2018Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
Институција/група
Poljoprivredni fakultetTY - CONF AU - Miletić, Dunja AU - Pantić, Milena AU - Lević, Steva AU - Matijašević, Danka AU - Sknepnek, Aleksandra AU - Pavlović, Vladimir AU - Nedović, Viktor AU - Nikšić, Miomir PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6833 AB - Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting body and mycelia are recognized as potential functional food and dietary supplement. On the market, there are already many products based on the fungi extracts. For certain types of mushrooms, submerged production of bioactive compounds stands out as the best method from the standpoint of industrial applications. Mushrooms are becoming an attractive functional food and overview about type of food suitable for enrichment with this valuable compounds were given. The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth (exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential. During fermentation residual glucose was quantified by HPLC. Mycelia products were characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative bacteria strains using broth microdilution method. Results showed that after 48h glucose concentration started to decrease and after day seven slowed down, when process was terminated saving energy and time. Applied gentle stirring during cultivation provided constant oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy pellets with long, branched and anastomosed hyphae of high density were formed. In prepared mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex chemical composition of methanol extracts comparing to EPS was noted. That might be the explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample showed significant activity against both Gram-positive and Gram-negative strains. Based on obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising process for bioactive compounds production. C3 - 2nd B-fost 2018 Congress 15-17 October Yerevan T1 - Mushrooms as food and supplements - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production UR - https://hdl.handle.net/21.15107/rcub_agrospace_6833 ER -
@conference{ author = "Miletić, Dunja and Pantić, Milena and Lević, Steva and Matijašević, Danka and Sknepnek, Aleksandra and Pavlović, Vladimir and Nedović, Viktor and Nikšić, Miomir", year = "2018", abstract = "Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting body and mycelia are recognized as potential functional food and dietary supplement. On the market, there are already many products based on the fungi extracts. For certain types of mushrooms, submerged production of bioactive compounds stands out as the best method from the standpoint of industrial applications. Mushrooms are becoming an attractive functional food and overview about type of food suitable for enrichment with this valuable compounds were given. The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth (exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential. During fermentation residual glucose was quantified by HPLC. Mycelia products were characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative bacteria strains using broth microdilution method. Results showed that after 48h glucose concentration started to decrease and after day seven slowed down, when process was terminated saving energy and time. Applied gentle stirring during cultivation provided constant oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy pellets with long, branched and anastomosed hyphae of high density were formed. In prepared mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex chemical composition of methanol extracts comparing to EPS was noted. That might be the explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample showed significant activity against both Gram-positive and Gram-negative strains. Based on obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising process for bioactive compounds production.", journal = "2nd B-fost 2018 Congress 15-17 October Yerevan", title = "Mushrooms as food and supplements - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6833" }
Miletić, D., Pantić, M., Lević, S., Matijašević, D., Sknepnek, A., Pavlović, V., Nedović, V.,& Nikšić, M.. (2018). Mushrooms as food and supplements - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production. in 2nd B-fost 2018 Congress 15-17 October Yerevan. https://hdl.handle.net/21.15107/rcub_agrospace_6833
Miletić D, Pantić M, Lević S, Matijašević D, Sknepnek A, Pavlović V, Nedović V, Nikšić M. Mushrooms as food and supplements - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production. in 2nd B-fost 2018 Congress 15-17 October Yerevan. 2018;. https://hdl.handle.net/21.15107/rcub_agrospace_6833 .
Miletić, Dunja, Pantić, Milena, Lević, Steva, Matijašević, Danka, Sknepnek, Aleksandra, Pavlović, Vladimir, Nedović, Viktor, Nikšić, Miomir, "Mushrooms as food and supplements - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production" in 2nd B-fost 2018 Congress 15-17 October Yerevan (2018), https://hdl.handle.net/21.15107/rcub_agrospace_6833 .