Isolation and characterization of autochthonous strains of lactic acid bacteria isolated from sjenica cheese
Izolacija i karakterizacija autohtonih sojeva bakterija mlečne kiseline izdvojenih iz sjeničkog sira
Apstrakt
Lactic acid bacteria strains isolated from autochtonous cheeses of Sjenica were researched. In those products, where starters were not added fermentation occurs as a result of natural flora present in the surrounding environment. Forty thermophilic, mesophilic and citrat+ LAB strains were isolated by selective mediums (MRS agar, LM17 agar and LDC agar) from 5 samples of Sjenica-cheese. These strains were exposed to the further analysis and indetification. After examination by Gram test and catalase test, 23 strains of LAB, were selected for further analysis. On the basis of lactose fermentation manner and acid production in 1% reconstituted skim milk, only 6 strains were selected. Determination of theese strains by API 50 CHL and Rapid ID 32 Strep tests, showed that 2 strains belong to the Lactococcus lactis ssp. lactis, 1 strain to the Lactococcus lactis ssp. cremoris, 1 strain to the Lactococcus lactis ssp. lactis bivar. diacetylactis and 2 strains to the Lactobacillus para. paracase...i.
U radu su izolovani i karakterisani sojevi bakterija mlečne kiseline izdvojeni iz sjeničkog sira, proizvedenog autohtonom tehnologijom, bez dodavanja starter kultura. Iz pet uzoraka sjeničkog sira izvršena je izolacija 40 termofilnih, mezofilnih i citrat+ sojeva BMK na odgovarajućim selektivnim podlogama (MRS agar, LM17 agar i LDC agar). Ovi sojevi su dalje podvrgnuti ispitivanju na Gram reakciju i katalaza test, posle čega je izabrano 23 soja za dalja ispitivanja. Na osnovu načina fermentacije laktoze i acidogene sposonosti praćene u 1% rekonstituisanom obranom mleku selekcionisano je samo šest sojeva. Determinacijom ovih sojeva pomoću API CHL 50 i Rapid ID 32 Strep testa, pokazalo se da dva soja pripadaju Lactococcus lactis ssp. lactis, jedan soj Lactococcus lactis ssp. cremoris jedan soj Lactococcus lactis ssp. lactis bivar. diacetylactis i dva soja Lactobacillus para. paracasei vrsti.
Ključne reči:
lactic acid bacteria / Sjenica cheese / bakterije mlečne kiseline / sjenički SirIzvor:
Prehrambena industrija - mleko i mlečni proizvodi, 2003, 14, 1-2, 142-142Izdavač:
- Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Radulović, Zorica AU - Martinović, Aleksandra AU - Obradović, Dragojlo B. PY - 2003 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/662 AB - Lactic acid bacteria strains isolated from autochtonous cheeses of Sjenica were researched. In those products, where starters were not added fermentation occurs as a result of natural flora present in the surrounding environment. Forty thermophilic, mesophilic and citrat+ LAB strains were isolated by selective mediums (MRS agar, LM17 agar and LDC agar) from 5 samples of Sjenica-cheese. These strains were exposed to the further analysis and indetification. After examination by Gram test and catalase test, 23 strains of LAB, were selected for further analysis. On the basis of lactose fermentation manner and acid production in 1% reconstituted skim milk, only 6 strains were selected. Determination of theese strains by API 50 CHL and Rapid ID 32 Strep tests, showed that 2 strains belong to the Lactococcus lactis ssp. lactis, 1 strain to the Lactococcus lactis ssp. cremoris, 1 strain to the Lactococcus lactis ssp. lactis bivar. diacetylactis and 2 strains to the Lactobacillus para. paracasei. AB - U radu su izolovani i karakterisani sojevi bakterija mlečne kiseline izdvojeni iz sjeničkog sira, proizvedenog autohtonom tehnologijom, bez dodavanja starter kultura. Iz pet uzoraka sjeničkog sira izvršena je izolacija 40 termofilnih, mezofilnih i citrat+ sojeva BMK na odgovarajućim selektivnim podlogama (MRS agar, LM17 agar i LDC agar). Ovi sojevi su dalje podvrgnuti ispitivanju na Gram reakciju i katalaza test, posle čega je izabrano 23 soja za dalja ispitivanja. Na osnovu načina fermentacije laktoze i acidogene sposonosti praćene u 1% rekonstituisanom obranom mleku selekcionisano je samo šest sojeva. Determinacijom ovih sojeva pomoću API CHL 50 i Rapid ID 32 Strep testa, pokazalo se da dva soja pripadaju Lactococcus lactis ssp. lactis, jedan soj Lactococcus lactis ssp. cremoris jedan soj Lactococcus lactis ssp. lactis bivar. diacetylactis i dva soja Lactobacillus para. paracasei vrsti. PB - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd T2 - Prehrambena industrija - mleko i mlečni proizvodi T1 - Isolation and characterization of autochthonous strains of lactic acid bacteria isolated from sjenica cheese T1 - Izolacija i karakterizacija autohtonih sojeva bakterija mlečne kiseline izdvojenih iz sjeničkog sira EP - 142 IS - 1-2 SP - 142 VL - 14 UR - https://hdl.handle.net/21.15107/rcub_agrospace_662 ER -
@article{ author = "Radulović, Zorica and Martinović, Aleksandra and Obradović, Dragojlo B.", year = "2003", abstract = "Lactic acid bacteria strains isolated from autochtonous cheeses of Sjenica were researched. In those products, where starters were not added fermentation occurs as a result of natural flora present in the surrounding environment. Forty thermophilic, mesophilic and citrat+ LAB strains were isolated by selective mediums (MRS agar, LM17 agar and LDC agar) from 5 samples of Sjenica-cheese. These strains were exposed to the further analysis and indetification. After examination by Gram test and catalase test, 23 strains of LAB, were selected for further analysis. On the basis of lactose fermentation manner and acid production in 1% reconstituted skim milk, only 6 strains were selected. Determination of theese strains by API 50 CHL and Rapid ID 32 Strep tests, showed that 2 strains belong to the Lactococcus lactis ssp. lactis, 1 strain to the Lactococcus lactis ssp. cremoris, 1 strain to the Lactococcus lactis ssp. lactis bivar. diacetylactis and 2 strains to the Lactobacillus para. paracasei., U radu su izolovani i karakterisani sojevi bakterija mlečne kiseline izdvojeni iz sjeničkog sira, proizvedenog autohtonom tehnologijom, bez dodavanja starter kultura. Iz pet uzoraka sjeničkog sira izvršena je izolacija 40 termofilnih, mezofilnih i citrat+ sojeva BMK na odgovarajućim selektivnim podlogama (MRS agar, LM17 agar i LDC agar). Ovi sojevi su dalje podvrgnuti ispitivanju na Gram reakciju i katalaza test, posle čega je izabrano 23 soja za dalja ispitivanja. Na osnovu načina fermentacije laktoze i acidogene sposonosti praćene u 1% rekonstituisanom obranom mleku selekcionisano je samo šest sojeva. Determinacijom ovih sojeva pomoću API CHL 50 i Rapid ID 32 Strep testa, pokazalo se da dva soja pripadaju Lactococcus lactis ssp. lactis, jedan soj Lactococcus lactis ssp. cremoris jedan soj Lactococcus lactis ssp. lactis bivar. diacetylactis i dva soja Lactobacillus para. paracasei vrsti.", publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd", journal = "Prehrambena industrija - mleko i mlečni proizvodi", title = "Isolation and characterization of autochthonous strains of lactic acid bacteria isolated from sjenica cheese, Izolacija i karakterizacija autohtonih sojeva bakterija mlečne kiseline izdvojenih iz sjeničkog sira", pages = "142-142", number = "1-2", volume = "14", url = "https://hdl.handle.net/21.15107/rcub_agrospace_662" }
Radulović, Z., Martinović, A.,& Obradović, D. B.. (2003). Isolation and characterization of autochthonous strains of lactic acid bacteria isolated from sjenica cheese. in Prehrambena industrija - mleko i mlečni proizvodi Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Savez hemičara i tehnologa, Beograd., 14(1-2), 142-142. https://hdl.handle.net/21.15107/rcub_agrospace_662
Radulović Z, Martinović A, Obradović DB. Isolation and characterization of autochthonous strains of lactic acid bacteria isolated from sjenica cheese. in Prehrambena industrija - mleko i mlečni proizvodi. 2003;14(1-2):142-142. https://hdl.handle.net/21.15107/rcub_agrospace_662 .
Radulović, Zorica, Martinović, Aleksandra, Obradović, Dragojlo B., "Isolation and characterization of autochthonous strains of lactic acid bacteria isolated from sjenica cheese" in Prehrambena industrija - mleko i mlečni proizvodi, 14, no. 1-2 (2003):142-142, https://hdl.handle.net/21.15107/rcub_agrospace_662 .