THE INFLUENCE OF DIET ON THE CONTENT OF ROUTINE PLANT IN BUCKWHEAT LEAVES

Abstract

Examined the content of the methanol extract routines MeOH dried buckwheat leaves were collected from several sites in the full flowering of plants. Extracts were prepared by healing with return cooler with the addition of methanol p.a. After extraction was performed filtration of extracts, prepared in such extracts was determined by the content of routine HPLC analysis method kvantotativne using external standards (rutin) over the calibration curve. Fagopyri herba drug is described in European Pharmacopceia VI as well as routine alanytical procedure content (Ph.Eur.6.0). Routine biennial examination content in leaves of buckwheat and collection of samples included four sites - Valjevo, Pancevo, Kucevo (Velika Plana) and Surduk. For the purposes of this study was selected local variety of buckwheat dove was grown in the system optimal mineral nutrition of plants with the addition of NPK nutrients hydrogel and zeolite to bind water in the surface layers of soil and prevent its evaporation loss. The content is varied routines and locations. At least it was routine in the locality listovima buckwheat. Surduk 2.78% and most of the experiments in Kucevo in Petrovac 3.71%. Isolated extraction procedure, routine use in the pharmaceutical and food industries.