POTENTIAL OF CHAMPIGNONS CULTIVATED ON DIGESTED ORGANIC WASTE IN THE PREVENTION AGAINST OXIDATIVE STRESS AND NEURAL DAMAGE

Abstract

Agaricus bisporus also known as champignon or white button mushroom was cultivated on digested organic waste. Its aqueous extract was investigated for the antioxidant potential, which could strengthen the protection of organism against oxidative stress damages. Likewise, acetylcholinesterase (AChE) inhibitory activity was observed. The crucial role of AChE in neural transmission makes it a primary target of a large number of cholinesterase-inhibiting drugs involved in the prevention of neurodegenerative diseases e.g. Alzheimer’s disease (AD). The antioxidant activities were evaluated by in vitro models including, 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging activity, prevention of lipid peroxidation (LPx) in a linoleic acid model system and chelating ability on ferrous ions. Based on the analysis, hot water extracts showed a typical carbohydrate pattern with the presence of polyphenols and small amounts of proteins. With regard to scavenging ability on DPPH radicals, the EC50 value for A. bisporus was 0.89 mg/ml, the EC50 value of the chelating abilities on ferrous ions was 1.38 mg/ml and for inhibition of LPx EC50 value was found to be 1.77 mg/ml. Extract of A. bisporus had a noticeable inhibition towards AChE, IC50=1.02 mg/ml and exerted weak toxicity against healthy human peripheral blood mononuclear cells (PBMC). More than 60% of cell viability was observed at 2 mg/ml. The good antioxidative properties and AChE inhibitory activity of A. bisporus aqueous extract make it suitable for everyday use as an inexpensive dietary supplement.