Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications
Аутори
Solarz, DariaWitko, Tomasz
Karcz, Robert
Malagurski, Ivana
Ponjavic, Marijana
Levic, Steva
Nesic, Aleksandra
Guzik, Maciej
Savic, Sanja
Nikodinovic-Runic, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be p...otentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.
Извор:
RSC advances, 2023, 13, 34, 24112-24128Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Solarz, Daria AU - Witko, Tomasz AU - Karcz, Robert AU - Malagurski, Ivana AU - Ponjavic, Marijana AU - Levic, Steva AU - Nesic, Aleksandra AU - Guzik, Maciej AU - Savic, Sanja AU - Nikodinovic-Runic, Jasmina PY - 2023 UR - http://www.ncbi.nlm.nih.gov/pubmed/37577093 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6430 AB - Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications. T2 - RSC advances T2 - RSC advancesRSC Adv T1 - Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications EP - 24128 IS - 34 SP - 24112 VL - 13 DO - 10.1039/d3ra03021k ER -
@article{ author = "Solarz, Daria and Witko, Tomasz and Karcz, Robert and Malagurski, Ivana and Ponjavic, Marijana and Levic, Steva and Nesic, Aleksandra and Guzik, Maciej and Savic, Sanja and Nikodinovic-Runic, Jasmina", year = "2023", abstract = "Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.", journal = "RSC advances, RSC advancesRSC Adv", title = "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications", pages = "24128-24112", number = "34", volume = "13", doi = "10.1039/d3ra03021k" }
Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavic, M., Levic, S., Nesic, A., Guzik, M., Savic, S.,& Nikodinovic-Runic, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications. in RSC advances, 13(34), 24112-24128. https://doi.org/10.1039/d3ra03021k
Solarz D, Witko T, Karcz R, Malagurski I, Ponjavic M, Levic S, Nesic A, Guzik M, Savic S, Nikodinovic-Runic J. Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications. in RSC advances. 2023;13(34):24112-24128. doi:10.1039/d3ra03021k .
Solarz, Daria, Witko, Tomasz, Karcz, Robert, Malagurski, Ivana, Ponjavic, Marijana, Levic, Steva, Nesic, Aleksandra, Guzik, Maciej, Savic, Sanja, Nikodinovic-Runic, Jasmina, "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications" in RSC advances, 13, no. 34 (2023):24112-24128, https://doi.org/10.1039/d3ra03021k . .