Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.)
Аутори
Terzić, DraganStanisavljević, Rade
Živanović, Tomislav
Tabaković, Marijenka
Trkulja, Nenad
Marković, Jordan
Poštić, Dobrivoj
Štrbanović, Ratibor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In order to have successful breeding, it is necessary to introduce new breeding material constantly and to use it through various types of hybridisation to increase the existing variability. Ten (10) lucerne varieties of different geographic origin were used in the study. Six varieties originated from the Republic of Serbia (Kruševačka 22, Kruševačka 28, NS-Banat ZMS II, NS-Mediana ZMS V, Zaječarska 83 and Čačanka 10), three varieties originated from the Republic of Croatia (Osječka 66, Osječka 88 and Osječka 99) and one variety originated from the Republika Srpska (Banjalučanka). A total of 100 seeds per each of 10 (ten) lucerne varieties were placed in Petri dishes to germinate. The dishes were placed in the seed germination chamber with the altering temperature of 20 oС in the dark for 16 h and 30 oС in the light for 8 h for seven days. The first green leaflets of lucerne seedlings (cotyledons) were used for the DNA extraction. The first and the second axes from the principal coordi...nates analysis accounted for a total of 63.1% of genetic variation, contained in the original dataset. It is clearly observed that the genotype Zaječarska 83 is genetically most distant from other studied lucerne genotypes. These studies confirmed that the observed collection of lucerne varieties is variable enough for the successful breeding process. Using an appropriate breeding model it is possible to breed varieties for certain purposes.
Извор:
Genetika, 2022, 54, 3, 1157-1169Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200010 (Институт за заштиту биља и животну средину, Београд) (RS-MESTD-inst-2020-200010)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200040 (Институт за кукуруз 'Земун поље', Београд-Земун) (RS-MESTD-inst-2020-200040)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200217 (Институт за крмно биље, Крушевац) (RS-MESTD-inst-2020-200217)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200383 (Универзитет у Нишу, Пољопривредни факултет, Крушевац) (RS-MESTD-inst-2020-200383)
URI
https://www.doiserbia.nb.rs/Article.aspx?id=0534-00122203157Thttp://aspace.agrif.bg.ac.rs/handle/123456789/6308
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Terzić, Dragan AU - Stanisavljević, Rade AU - Živanović, Tomislav AU - Tabaković, Marijenka AU - Trkulja, Nenad AU - Marković, Jordan AU - Poštić, Dobrivoj AU - Štrbanović, Ratibor PY - 2022 UR - https://www.doiserbia.nb.rs/Article.aspx?id=0534-00122203157T UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6308 AB - In order to have successful breeding, it is necessary to introduce new breeding material constantly and to use it through various types of hybridisation to increase the existing variability. Ten (10) lucerne varieties of different geographic origin were used in the study. Six varieties originated from the Republic of Serbia (Kruševačka 22, Kruševačka 28, NS-Banat ZMS II, NS-Mediana ZMS V, Zaječarska 83 and Čačanka 10), three varieties originated from the Republic of Croatia (Osječka 66, Osječka 88 and Osječka 99) and one variety originated from the Republika Srpska (Banjalučanka). A total of 100 seeds per each of 10 (ten) lucerne varieties were placed in Petri dishes to germinate. The dishes were placed in the seed germination chamber with the altering temperature of 20 oС in the dark for 16 h and 30 oС in the light for 8 h for seven days. The first green leaflets of lucerne seedlings (cotyledons) were used for the DNA extraction. The first and the second axes from the principal coordinates analysis accounted for a total of 63.1% of genetic variation, contained in the original dataset. It is clearly observed that the genotype Zaječarska 83 is genetically most distant from other studied lucerne genotypes. These studies confirmed that the observed collection of lucerne varieties is variable enough for the successful breeding process. Using an appropriate breeding model it is possible to breed varieties for certain purposes. T2 - Genetika T2 - Genetika T1 - Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.) EP - 1169 IS - 3 SP - 1157 VL - 54 DO - 10.2298/GENSR2203157T ER -
@article{ author = "Terzić, Dragan and Stanisavljević, Rade and Živanović, Tomislav and Tabaković, Marijenka and Trkulja, Nenad and Marković, Jordan and Poštić, Dobrivoj and Štrbanović, Ratibor", year = "2022", abstract = "In order to have successful breeding, it is necessary to introduce new breeding material constantly and to use it through various types of hybridisation to increase the existing variability. Ten (10) lucerne varieties of different geographic origin were used in the study. Six varieties originated from the Republic of Serbia (Kruševačka 22, Kruševačka 28, NS-Banat ZMS II, NS-Mediana ZMS V, Zaječarska 83 and Čačanka 10), three varieties originated from the Republic of Croatia (Osječka 66, Osječka 88 and Osječka 99) and one variety originated from the Republika Srpska (Banjalučanka). A total of 100 seeds per each of 10 (ten) lucerne varieties were placed in Petri dishes to germinate. The dishes were placed in the seed germination chamber with the altering temperature of 20 oС in the dark for 16 h and 30 oС in the light for 8 h for seven days. The first green leaflets of lucerne seedlings (cotyledons) were used for the DNA extraction. The first and the second axes from the principal coordinates analysis accounted for a total of 63.1% of genetic variation, contained in the original dataset. It is clearly observed that the genotype Zaječarska 83 is genetically most distant from other studied lucerne genotypes. These studies confirmed that the observed collection of lucerne varieties is variable enough for the successful breeding process. Using an appropriate breeding model it is possible to breed varieties for certain purposes.", journal = "Genetika, Genetika", title = "Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.)", pages = "1169-1157", number = "3", volume = "54", doi = "10.2298/GENSR2203157T" }
Terzić, D., Stanisavljević, R., Živanović, T., Tabaković, M., Trkulja, N., Marković, J., Poštić, D.,& Štrbanović, R.. (2022). Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.). in Genetika, 54(3), 1157-1169. https://doi.org/10.2298/GENSR2203157T
Terzić D, Stanisavljević R, Živanović T, Tabaković M, Trkulja N, Marković J, Poštić D, Štrbanović R. Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.). in Genetika. 2022;54(3):1157-1169. doi:10.2298/GENSR2203157T .
Terzić, Dragan, Stanisavljević, Rade, Živanović, Tomislav, Tabaković, Marijenka, Trkulja, Nenad, Marković, Jordan, Poštić, Dobrivoj, Štrbanović, Ratibor, "Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.)" in Genetika, 54, no. 3 (2022):1157-1169, https://doi.org/10.2298/GENSR2203157T . .