A biochemical and proteomic approach to the analysis of tomato mutant fruit growth [Biohemijski i proteomički pristup u analizi rastenja plodova mutanta paradajza]
2021
Преузимање 🢃
Аутори
Marjanović, MilenaJovanović, Zorica
Vucelić Radović, Biljana
Savić, Sladjana
Petrović, Ivana
Stikić, Radmila
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
To assess the effects of ABA deficiency on tomato fruit growth, the ABA mutant flacca was grown in an optimal soil water regime and various analyzes were performed, including morphological (fruit number, diameter and fruit biomass), physiological (duration of growth and fruit growth rate), biochemical (ABA accumulation, enzyme cell wall peroxidase activity) as well as proteomics. The fruit growth analysis showed that the slower fruit growth rate and development resulted in smaller flacca fruits in comparison to the wild-type fruits. The comparison of the temporal dynamics of cell wall peroxidase activity and ABA content in our experiment indicated an opposite relationship during fruit development. Proteomic analysis and the down-regulation of most proteins from carbon and amino acid metabolism, the translation and processing of proteins, energy metabolism and cell wall-related metabolism in the flacca fruits compared to the wild type, indicated reduced metabolic flux which reflected a ...slower fruit growth and development and reduced fruit size in the ABA mutant. These findings also indicated that ABA limited carbon sources, which could be responsible for the reduced fruit growth and size of ABA-deficient tomato fruits. The up-regulation of sulfur and oxygen-evolving enhancer proteins in the flacca fruits implicated the maintenance of photosynthesis in the late expansion phase, which slows down transition to the ripening stage. The majority of antioxidative and stress defence proteins were down-regulated in the flacca fruits, which could be related to the role of ABA in the activity of different antioxidative enzymes as well as in regulating cell wall expansion and the cessation of fruit growth.
Кључне речи:
ABA / cell wall peroxidase / flacca mutantИзвор:
Botanica Serbica, 2021, 45, 1, 71-85Издавач:
- Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
DOI: 10.2298/BOTSERB2101071M
ISSN: 1821-2158
WoS: 000641742900007
Scopus: 2-s2.0-85105030360
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Marjanović, Milena AU - Jovanović, Zorica AU - Vucelić Radović, Biljana AU - Savić, Sladjana AU - Petrović, Ivana AU - Stikić, Radmila PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5847 AB - To assess the effects of ABA deficiency on tomato fruit growth, the ABA mutant flacca was grown in an optimal soil water regime and various analyzes were performed, including morphological (fruit number, diameter and fruit biomass), physiological (duration of growth and fruit growth rate), biochemical (ABA accumulation, enzyme cell wall peroxidase activity) as well as proteomics. The fruit growth analysis showed that the slower fruit growth rate and development resulted in smaller flacca fruits in comparison to the wild-type fruits. The comparison of the temporal dynamics of cell wall peroxidase activity and ABA content in our experiment indicated an opposite relationship during fruit development. Proteomic analysis and the down-regulation of most proteins from carbon and amino acid metabolism, the translation and processing of proteins, energy metabolism and cell wall-related metabolism in the flacca fruits compared to the wild type, indicated reduced metabolic flux which reflected a slower fruit growth and development and reduced fruit size in the ABA mutant. These findings also indicated that ABA limited carbon sources, which could be responsible for the reduced fruit growth and size of ABA-deficient tomato fruits. The up-regulation of sulfur and oxygen-evolving enhancer proteins in the flacca fruits implicated the maintenance of photosynthesis in the late expansion phase, which slows down transition to the ripening stage. The majority of antioxidative and stress defence proteins were down-regulated in the flacca fruits, which could be related to the role of ABA in the activity of different antioxidative enzymes as well as in regulating cell wall expansion and the cessation of fruit growth. PB - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade T2 - Botanica Serbica T1 - A biochemical and proteomic approach to the analysis of tomato mutant fruit growth [Biohemijski i proteomički pristup u analizi rastenja plodova mutanta paradajza] EP - 85 IS - 1 SP - 71 VL - 45 DO - 10.2298/BOTSERB2101071M ER -
@article{ author = "Marjanović, Milena and Jovanović, Zorica and Vucelić Radović, Biljana and Savić, Sladjana and Petrović, Ivana and Stikić, Radmila", year = "2021", abstract = "To assess the effects of ABA deficiency on tomato fruit growth, the ABA mutant flacca was grown in an optimal soil water regime and various analyzes were performed, including morphological (fruit number, diameter and fruit biomass), physiological (duration of growth and fruit growth rate), biochemical (ABA accumulation, enzyme cell wall peroxidase activity) as well as proteomics. The fruit growth analysis showed that the slower fruit growth rate and development resulted in smaller flacca fruits in comparison to the wild-type fruits. The comparison of the temporal dynamics of cell wall peroxidase activity and ABA content in our experiment indicated an opposite relationship during fruit development. Proteomic analysis and the down-regulation of most proteins from carbon and amino acid metabolism, the translation and processing of proteins, energy metabolism and cell wall-related metabolism in the flacca fruits compared to the wild type, indicated reduced metabolic flux which reflected a slower fruit growth and development and reduced fruit size in the ABA mutant. These findings also indicated that ABA limited carbon sources, which could be responsible for the reduced fruit growth and size of ABA-deficient tomato fruits. The up-regulation of sulfur and oxygen-evolving enhancer proteins in the flacca fruits implicated the maintenance of photosynthesis in the late expansion phase, which slows down transition to the ripening stage. The majority of antioxidative and stress defence proteins were down-regulated in the flacca fruits, which could be related to the role of ABA in the activity of different antioxidative enzymes as well as in regulating cell wall expansion and the cessation of fruit growth.", publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade", journal = "Botanica Serbica", title = "A biochemical and proteomic approach to the analysis of tomato mutant fruit growth [Biohemijski i proteomički pristup u analizi rastenja plodova mutanta paradajza]", pages = "85-71", number = "1", volume = "45", doi = "10.2298/BOTSERB2101071M" }
Marjanović, M., Jovanović, Z., Vucelić Radović, B., Savić, S., Petrović, I.,& Stikić, R.. (2021). A biochemical and proteomic approach to the analysis of tomato mutant fruit growth [Biohemijski i proteomički pristup u analizi rastenja plodova mutanta paradajza]. in Botanica Serbica Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 45(1), 71-85. https://doi.org/10.2298/BOTSERB2101071M
Marjanović M, Jovanović Z, Vucelić Radović B, Savić S, Petrović I, Stikić R. A biochemical and proteomic approach to the analysis of tomato mutant fruit growth [Biohemijski i proteomički pristup u analizi rastenja plodova mutanta paradajza]. in Botanica Serbica. 2021;45(1):71-85. doi:10.2298/BOTSERB2101071M .
Marjanović, Milena, Jovanović, Zorica, Vucelić Radović, Biljana, Savić, Sladjana, Petrović, Ivana, Stikić, Radmila, "A biochemical and proteomic approach to the analysis of tomato mutant fruit growth [Biohemijski i proteomički pristup u analizi rastenja plodova mutanta paradajza]" in Botanica Serbica, 45, no. 1 (2021):71-85, https://doi.org/10.2298/BOTSERB2101071M . .